Rorug04G0359000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005117
Physical Location & Seq
Reverse (-)
53172323 .. 53176539
4217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0359000.1

Sequence Viewer

Length: 1530 bp
ATGGCCGAATTTAGGGTTTACAACTCGATGACGCAGCAGAAGGAGGTCTTCAAGCCCAACGTTCCCGGCGAGGTCAGTATGTACGTCTGCGGCGTCACCGCCTACGATTTCAGCCACATCGGCCACGCCCGCGCCGCCGTCAACTTTGATGTCCTCTTCAGATACTTAACGGAGCTGGGTTATAAGGTCACATACGTGAGGAACTTTACCGATGTTGATGACAAGATAATTCATAAAGCAAATGAGATTGGGGAAGATCCATTAAGTTTAAGTAATCGTTTTTGTCAAGAATATCTCAATGACATGGGAGATCTCCAATGCCTCACTCCAACCTATCAGCCGCGTGTTTCTGATCACATGGAACAGATAATAGATATGATAAAACAGATCATCAACAATGACTATGCCTACGTAGTTGATGGAGATGTATTCTTTGCTGTTGAGAAATTCCCAAATTATGGGCAGTTATCTGGACAAAAGTTGGAACATATTAGGGCGGGTGAACGAGTTTCTGTTGATTCAAGAAAACGCAATCCTGCAGACTTTGCTTTGTGGAAGTCTGCAAAGCCTGGAGAGCCAAGTTGGGACAGCCCTTGGGGACTAGGAAGGCCGGGGTGGCACATAGAATGCAGTGCCATGAGTGCTCATTATCTTACGTTCAAGTTTGATATTCACGGTGGTGGTATTGACTTGATTTTTCCACATCATGAAAATGAGATTGCTCAAAGTGGTGCTGCATACCAGGAGGGCTGTGTCAGTTATTGGTTGCACAACGGTCATGTTACTAATAATAATGAGAAAATGTCAAAATCATTGGGAAACTTTTTTACAATTCGTGAGATTACTGAACGGTATCATCCCCTGTCTTTGAGATACTTCTTGTTAAGTGCACACTATAGAACTCCTCTCAATTATAGTGTCTCCCAGCTGGAAGGTGCATCGGATGCAGTGTATTACATATATAATACTTTACAAGAATGTGAGGATGCTTTATCTCCATTTCAAGATGGGGCCCAAAAGGAAGGTGCAGAACAAAATGGTAGAACAGTTAGAATTACTCAAGAAGCTGAAGTATTGATCAGTAAGTTAAAGAAGGAATTTGAGGCTAAAATGTCTGATGACTTGAACTCTGCAAACATATTGACCGGTGCTTTTCAAGATGCCTTGAGATTTATAAACACATCTTTGAAACCACTCAAGAAGCATCCGAAGCAACAGCAATTATACAAGTCACTAGTTGAAACAGTGAAAGCTGTTAAAAAAGTTCTTGACATTTTGGGTTTGCTATCCTCCCTTACATACTCTGAGGTAGTGCAGCAATTGAAGGCTAAGGCGTTGAAGAGGGCTGGACTTTCTGAAGATGACGTGCTGCGTACAATTGCAGACAGGACATTAGCAAGGAAAAACAAGGATTATGCAAAAAGCGATCAGATCAGAGTCGAAATGGAGGAAAAGGGTATTCAAATCATGGATGTGGGCAATGAGAGTATCTGGAGACCTCGTGTCCCAAAAGAACCAGAACAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

509

Amino Acids

58.01

Weight (kDa)

6.05

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1e PF01406 17 - 313 4e-130 tRNA synthetases class I (C) catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 183, 1175
AccB7I CCANNNNNTGG 1 cut(s) 458
AccII CGCG 2 cut(s) 132, 343
AciI CCGC 6 cut(s) 90, 99, 130, 135, 341, 497
AclI AACGTT 1 cut(s) 60
AclWI GGATC 1 cut(s) 251
AcoI YGGCCR 2 cut(s) 3, 121
AcsI RAATTY 3 cut(s) 8, 446, 1097
AcuI CTGAAG 3 cut(s) 142, 1089, 1377
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 2 cut(s) 83, 1375
AfiI CCNNNNNNNGG 2 cut(s) 12, 458
AgeI ACCGGT 1 cut(s) 1145
AhdI GACNNNNNGTC 1 cut(s) 1502
AhlI ACTAGT 1 cut(s) 1234
AjiI CACGTC 1 cut(s) 1366
AjnI CCWGG 2 cut(s) 568, 741
AleI CACNNNNGTG 2 cut(s) 194, 678
AluBI AGCT 4 cut(s) 175, 928, 1067, 1253
AluI AGCT 4 cut(s) 175, 928, 1067, 1253
Alw21I GWGCWC 2 cut(s) 646, 892
Alw26I GTCTC 2 cut(s) 925, 1489
Alw44I GTGCAC 1 cut(s) 888
AlwI GGATC 1 cut(s) 251
AoxI GGCC 4 cut(s) 3, 121, 608, 1011
ApaI GGGCCC 1 cut(s) 1015
ApaLI GTGCAC 1 cut(s) 888
ApeKI GCWGC 4 cut(s) 34, 734, 1315, 1369
ApoI RAATTY 3 cut(s) 8, 446, 1097
AsiGI ACCGGT 1 cut(s) 1145
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 2 cut(s) 1011, 1012
AsuC2I CCSGG 2 cut(s) 66, 612
AsuHPI GGTGA 2 cut(s) 88, 512
BaeGI GKGCMC 2 cut(s) 892, 1015
BanII GRGCYC 1 cut(s) 1015
BauI CACGAG 1 cut(s) 1500
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 2 cut(s) 646, 892
BbvI GCAGC 4 cut(s) 46, 721, 1327, 1356
BccI CCATC 2 cut(s) 413, 1001
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 2 cut(s) 570, 743
BclI TGATCA 2 cut(s) 352, 1077
BcnI CCSGG 2 cut(s) 66, 612
BcoDI GTCTC 2 cut(s) 925, 1489
BcuI ACTAGT 1 cut(s) 1234
BfaI CTAG 2 cut(s) 602, 1235
BfmI CTRYAG 2 cut(s) 537, 895
BglI GCCNNNNNGGC 2 cut(s) 120, 616
BglII AGATCT 1 cut(s) 310
BisI GCNGC 7 cut(s) 35, 91, 135, 341, 735, 1316, 1370
BlsI GCNGC 7 cut(s) 36, 92, 136, 342, 736, 1317, 1371
Bme1390I CCNGG 4 cut(s) 66, 570, 612, 743
BmeRI GACNNNNNGTC 1 cut(s) 1502
BmgBI CACGTC 1 cut(s) 1366
BmgT120I GGNCC 2 cut(s) 1011, 1012
BmiI GGNNCC 2 cut(s) 1012, 1013
BmrFI CCNGG 4 cut(s) 66, 570, 612, 743
BmsI GCATC 5 cut(s) 934, 947, 976, 1150, 1213
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 2 cut(s) 591, 1513
Bpu10I CCTNAGC 1 cut(s) 1329
BpuEI CTTGAG 3 cut(s) 1044, 1181, 1186
BpuMI CCSGG 2 cut(s) 66, 612
BsaAI YACGTR 2 cut(s) 196, 412
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 1489
BsaJI CCNNGG 2 cut(s) 593, 611
BsaWI WCCGGW 1 cut(s) 1145
BsaXI ACNNNNNCTCC 2 cut(s) 1486, 1516
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 458
Bse118I RCCGGY 1 cut(s) 1145
Bse3DI GCAATG 1 cut(s) 1486
BseBI CCWGG 2 cut(s) 570, 743
BseDI CCNNGG 2 cut(s) 593, 611
BseGI GGATG 5 cut(s) 856, 949, 991, 1204, 1477
BseLI CCNNNNNNNGG 2 cut(s) 12, 458
BseMI GCAATG 1 cut(s) 1486
BseMII CTCAG 1 cut(s) 1296
BseRI GAGGAG 1 cut(s) 894
BseSI GKGCMC 2 cut(s) 892, 1015
BseXI GCAGC 4 cut(s) 46, 721, 1327, 1356
BseYI CCCAGC 2 cut(s) 175, 924
BsgI GTGCAG 2 cut(s) 1047, 1334
Bsh1236I CGCG 2 cut(s) 132, 343
BshFI GGCC 4 cut(s) 5, 123, 610, 1013
BshTI ACCGGT 1 cut(s) 1145
BsiHKAI GWGCWC 2 cut(s) 646, 892
BsiSI CCGG 3 cut(s) 66, 611, 1146
BslFI GGGAC 3 cut(s) 599, 612, 1490
BslI CCNNNNNNNGG 2 cut(s) 12, 458
BsmAI GTCTC 2 cut(s) 925, 1489
BsmFI GGGAC 3 cut(s) 599, 612, 1490
BsmI GAATGC 1 cut(s) 632
BsnI GGCC 4 cut(s) 5, 123, 610, 1013
Bso31I GGTCTC 1 cut(s) 1489
Bsp120I GGGCCC 1 cut(s) 1011
Bsp1286I GDGCHC 3 cut(s) 646, 892, 1015
Bsp143I GATC 7 cut(s) 256, 310, 352, 387, 1077, 1426, 1431
BspACI CCGC 6 cut(s) 90, 99, 130, 135, 341, 497
BspANI GGCC 4 cut(s) 5, 123, 610, 1013
BspCNI CTCAG 1 cut(s) 1297
BspFNI CGCG 2 cut(s) 132, 343
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 2 cut(s) 1012, 1013
BspMAI CTGCAG 1 cut(s) 541
BspPI GGATC 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 1489
BsrDI GCAATG 1 cut(s) 1486
BsrFI RCCGGY 1 cut(s) 1145
BssAI RCCGGY 1 cut(s) 1145
BssECI CCNNGG 2 cut(s) 593, 611
BssMI GATC 7 cut(s) 256, 310, 352, 387, 1077, 1426, 1431
BssNI GRCGYC 1 cut(s) 93
BssSI CACGAG 1 cut(s) 1500
BssT1I CCWWGG 1 cut(s) 593
Bst2BI CACGAG 1 cut(s) 1500
Bst2UI CCWGG 2 cut(s) 570, 743
Bst4CI ACNGT 5 cut(s) 677, 776, 852, 1048, 1246
Bst6I CTCTTC 2 cut(s) 161, 1334
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 2 cut(s) 545, 944
BstBAI YACGTR 2 cut(s) 196, 412
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 2 cut(s) 1305, 1329
BstF5I GGATG 5 cut(s) 856, 949, 991, 1204, 1477
BstFNI CGCG 2 cut(s) 132, 343
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 7 cut(s) 259, 313, 355, 390, 1080, 1429, 1434
BstMAI GTCTC 2 cut(s) 925, 1489
BstMBI GATC 7 cut(s) 256, 310, 352, 387, 1077, 1426, 1431
BstMWI GCNNNNNNNGC 9 cut(s) 120, 129, 134, 545, 574, 616, 641, 944, 1210
BstNI CCWGG 2 cut(s) 570, 743
BstSCI CCNGG 4 cut(s) 64, 568, 610, 741
BstSFI CTRYAG 2 cut(s) 537, 895
BstSLI GKGCMC 2 cut(s) 892, 1015
BstSNI TACGTA 1 cut(s) 412
BstUI CGCG 2 cut(s) 132, 343
BstV1I GCAGC 4 cut(s) 46, 721, 1327, 1356
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 2 cut(s) 256, 310
BstYI RGATCY 2 cut(s) 256, 310
BsuRI GGCC 4 cut(s) 5, 123, 610, 1013
BtrI CACGTC 1 cut(s) 1366
BtsCI GGATG 5 cut(s) 856, 949, 991, 1204, 1477
BtsI GCAGTG 2 cut(s) 637, 954
BtsIMutI CAGTG 3 cut(s) 637, 954, 1251
Cac8I GCNNGC 1 cut(s) 130
CciI TCATGA 1 cut(s) 706
CfoI GCGC 1 cut(s) 134
Cfr10I RCCGGY 1 cut(s) 1145
Cfr13I GGNCC 2 cut(s) 1011, 1012
CseI GACGC 2 cut(s) 40, 82
Csp6I GTAC 2 cut(s) 82, 1374
CspAI ACCGGT 1 cut(s) 1145
CviAII CATG 6 cut(s) 304, 358, 637, 707, 779, 1468
CviQI GTAC 2 cut(s) 82, 1374
DdeI CTNAG 2 cut(s) 1305, 1329
DpnI GATC 7 cut(s) 258, 312, 354, 389, 1079, 1428, 1433
DpnII GATC 7 cut(s) 256, 310, 352, 387, 1077, 1426, 1431
DriI GACNNNNNGTC 1 cut(s) 1502
EaeI YGGCCR 2 cut(s) 3, 121
Eam1104I CTCTTC 2 cut(s) 161, 1334
Eam1105I GACNNNNNGTC 1 cut(s) 1502
EarI CTCTTC 2 cut(s) 161, 1334
Eco105I TACGTA 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 593
Eco24I GRGCYC 1 cut(s) 1015
Eco31I GGTCTC 1 cut(s) 1489
Eco57I CTGAAG 3 cut(s) 142, 1089, 1377
EcoO109I RGGNCCY 1 cut(s) 1011
EcoRII CCWGG 2 cut(s) 568, 741
EcoT14I CCWWGG 1 cut(s) 593
EcoT38I GRGCYC 1 cut(s) 1015
ErhI CCWWGG 1 cut(s) 593
FaeI CATG 6 cut(s) 307, 361, 640, 710, 782, 1471
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FaqI GGGAC 3 cut(s) 599, 612, 1490
FatI CATG 6 cut(s) 303, 357, 636, 706, 778, 1467
FauI CCCGC 2 cut(s) 137, 490
FbaI TGATCA 2 cut(s) 352, 1077
Fnu4HI GCNGC 7 cut(s) 35, 91, 135, 341, 735, 1316, 1370
FokI GGATG 5 cut(s) 843, 956, 998, 1191, 1484
FriOI GRGCYC 1 cut(s) 1015
Fsp4HI GCNGC 7 cut(s) 35, 91, 135, 341, 735, 1316, 1370
FspBI CTAG 2 cut(s) 602, 1235
GlaI GCGC 1 cut(s) 133
GluI GCNGC 7 cut(s) 35, 91, 135, 341, 735, 1316, 1370
GsaI CCCAGC 2 cut(s) 179, 928
GsuI CTGGAG 2 cut(s) 591, 1513
HaeIII GGCC 4 cut(s) 5, 123, 610, 1013
HapII CCGG 3 cut(s) 66, 611, 1146
HgaI GACGC 2 cut(s) 40, 82
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 6 cut(s) 307, 361, 640, 710, 782, 1471
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 2 cut(s) 518, 1437
HpaII CCGG 3 cut(s) 66, 611, 1146
HphI GGTGA 2 cut(s) 88, 512
Hpy166II GTNNAC 4 cut(s) 19, 142, 503, 890
Hpy188I TCNGA 9 cut(s) 161, 352, 943, 1117, 1209, 1306, 1357, 1431, 1436
Hpy8I GTNNAC 4 cut(s) 19, 142, 503, 890
HpyAV CCTTC 6 cut(s) 34, 600, 926, 1016, 1087, 1318
HpyCH4III ACNGT 5 cut(s) 677, 776, 852, 1048, 1246
HpyCH4IV ACGT 6 cut(s) 60, 84, 195, 411, 656, 1365
HpyF10VI GCNNNNNNNGC 9 cut(s) 120, 129, 134, 545, 574, 616, 641, 944, 1210
HpyF3I CTNAG 2 cut(s) 1305, 1329
HpySE526I ACGT 6 cut(s) 60, 84, 195, 411, 656, 1365
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 6 cut(s) 307, 361, 640, 710, 782, 1471
HspAI GCGC 1 cut(s) 132
Ksp22I TGATCA 2 cut(s) 352, 1077
Kzo9I GATC 7 cut(s) 256, 310, 352, 387, 1077, 1426, 1431
LmnI GCTCC 1 cut(s) 172
Lsp1109I GCAGC 4 cut(s) 46, 721, 1327, 1356
LweI GCATC 5 cut(s) 934, 947, 976, 1150, 1213
MaeI CTAG 2 cut(s) 602, 1235
MaeII ACGT 6 cut(s) 60, 84, 195, 411, 656, 1365
MaeIII GTNAC 4 cut(s) 94, 187, 781, 1230
MalI GATC 7 cut(s) 258, 312, 354, 389, 1079, 1428, 1433
MboI GATC 7 cut(s) 256, 310, 352, 387, 1077, 1426, 1431
MboII GAAGA 5 cut(s) 40, 148, 266, 1351, 1370
MfeI CAATTG 2 cut(s) 1319, 1377
MflI RGATCY 2 cut(s) 256, 310
MhlI GDGCHC 3 cut(s) 646, 892, 1015
MlyI GAGTC 1 cut(s) 1446
MmeI TCCRAC 2 cut(s) 353, 462
MseI TTAA 6 cut(s) 167, 263, 269, 884, 1088, 1257
MslI CAYNNNNRTG 4 cut(s) 194, 678, 711, 1472
MspA1I CMGCKG 1 cut(s) 928
MspI CCGG 3 cut(s) 66, 611, 1146
MspR9I CCNGG 4 cut(s) 66, 570, 612, 743
MunI CAATTG 2 cut(s) 1319, 1377
Mva1269I GAATGC 1 cut(s) 632
MvaI CCWGG 2 cut(s) 570, 743
MvnI CGCG 2 cut(s) 132, 343
MwoI GCNNNNNNNGC 9 cut(s) 120, 129, 134, 545, 574, 616, 641, 944, 1210
NciI CCSGG 2 cut(s) 66, 612
NdeII GATC 7 cut(s) 256, 310, 352, 387, 1077, 1426, 1431
NlaIII CATG 6 cut(s) 307, 361, 640, 710, 782, 1471
NlaIV GGNNCC 2 cut(s) 1012, 1013
NmuCI GTSAC 3 cut(s) 94, 187, 1230
OliI CACNNNNGTG 2 cut(s) 194, 678
PagI TCATGA 1 cut(s) 706
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 90
PctI GAATGC 1 cut(s) 632
PfeI GAWTC 1 cut(s) 518
PflMI CCANNNNNTGG 1 cut(s) 458
PinAI ACCGGT 1 cut(s) 1145
PkrI GCNGC 7 cut(s) 36, 92, 136, 342, 736, 1317, 1371
PleI GAGTC 1 cut(s) 1445
PpsI GAGTC 1 cut(s) 1445
Ppu21I YACGTR 2 cut(s) 196, 412
PsiI TTATAA 2 cut(s) 183, 1175
Psp1406I AACGTT 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 568, 741
PspFI CCCAGC 2 cut(s) 175, 924
PspGI CCWGG 2 cut(s) 568, 741
PspN4I GGNNCC 2 cut(s) 1012, 1013
PspOMI GGGCCC 1 cut(s) 1011
PspPI GGNCC 2 cut(s) 1011, 1012
PstI CTGCAG 1 cut(s) 541
PsuI RGATCY 2 cut(s) 256, 310
PvuII CAGCTG 1 cut(s) 928
RsaI GTAC 2 cut(s) 83, 1375
RsaNI GTAC 2 cut(s) 82, 1374
RseI CAYNNNNRTG 4 cut(s) 194, 678, 711, 1472
SaqAI TTAA 6 cut(s) 167, 263, 269, 884, 1088, 1257
SatI GCNGC 7 cut(s) 35, 91, 135, 341, 735, 1316, 1370
Sau3AI GATC 7 cut(s) 256, 310, 352, 387, 1077, 1426, 1431
Sau96I GGNCC 2 cut(s) 1011, 1012
SchI GAGTC 1 cut(s) 1446
ScrFI CCNGG 4 cut(s) 66, 570, 612, 743
SduI GDGCHC 3 cut(s) 646, 892, 1015
SfaNI GCATC 5 cut(s) 934, 947, 976, 1150, 1213
SfcI CTRYAG 2 cut(s) 537, 895
SmiMI CAYNNNNRTG 4 cut(s) 194, 678, 711, 1472
SmlI CTYRAG 3 cut(s) 1059, 1165, 1196
SmoI CTYRAG 3 cut(s) 1059, 1165, 1196
SnaBI TACGTA 1 cut(s) 412
SpeI ACTAGT 1 cut(s) 1234
SsiI CCGC 6 cut(s) 90, 99, 130, 135, 341, 497
SspMI CTAG 2 cut(s) 602, 1235
StyD4I CCNGG 4 cut(s) 64, 568, 610, 741
StyI CCWWGG 1 cut(s) 593
TaaI ACNGT 5 cut(s) 677, 776, 852, 1048, 1246
TaiI ACGT 6 cut(s) 63, 87, 198, 414, 659, 1368
TaqI TCGA 2 cut(s) 26, 1440
TauI GCSGC 3 cut(s) 93, 137, 343
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 6 cut(s) 167, 263, 269, 884, 1088, 1257
Tru9I TTAA 6 cut(s) 167, 263, 269, 884, 1088, 1257
TscAI CASTG 3 cut(s) 637, 954, 1251
TseFI GTSAC 3 cut(s) 94, 187, 1230
TseI GCWGC 4 cut(s) 34, 734, 1315, 1369
Tsp45I GTSAC 3 cut(s) 94, 187, 1230
TspDTI ATGAA 2 cut(s) 221, 723
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 3 cut(s) 637, 954, 1251
Van91I CCANNNNNTGG 1 cut(s) 458
VneI GTGCAC 1 cut(s) 888
XapI RAATTY 3 cut(s) 8, 446, 1097
XspI CTAG 2 cut(s) 602, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.