Rorug05G0178000

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006724
Physical Location & Seq
Reverse (-)
16617878 .. 16619172
1295 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0178000.1

Sequence Viewer

Length: 585 bp
ATGGACGAGTTGCATCAGGCAGCTTCAGCATATTACAACAACGGGTCGCCTGAGATTCAGCAACTAGCATGGAGCTTCTTCCAATCCATGGACACAAACTGCGACGGCCAAGTTGACTGGAACGAGTTCTCTGCATTTCTCTACCAAAGCGGCAACCAATGGATCAGCCCCAGCTTCTTCAACGATCTGGACCGGAACCACGACGGTGCTCTCGATTTCATGGAAGTTCTCACGTTTTACTATATCATCAAGACTAGGAGCTTCTGGTGCAGCGTCTGCGGGATTTGTCTCACGGGGCTCTACTTCACTTGTGTTGAGTGCTTTGACAACGTGAACTCAAACACTTACGATCTTTGTCCCAGATGCTATGAGTCGAAGCCGGAAAGGCAAAGGTATAACCAGCAGAGTGATCACCATCATGACTACTTTATGGATAACCATATGTTGCTCAGATCAAAGAGAGGAGGATATGGTTCTGCAAACCTGGCCATAGATTCACCTGGAGGTGGACCAGGACGGGTATCGTCCCTAAACTATGCTGCCAAGGCCAATTTGATACCCGAACTCTCAAAAGTATCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

22.18

Weight (kDa)

4.95

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 21 - 81 1.4e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 2 cut(s) 150, 279
AclWI GGATC 1 cut(s) 170
AcoI YGGCCR 2 cut(s) 106, 486
AcuI CTGAAG 1 cut(s) 9
AfiI CCNNNNNNNGG 2 cut(s) 88, 506
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 3 cut(s) 483, 499, 511
AleI CACNNNNGTG 1 cut(s) 204
AluBI AGCT 4 cut(s) 23, 75, 174, 261
AluI AGCT 4 cut(s) 23, 75, 174, 261
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 276
AoxI GGCC 3 cut(s) 106, 486, 546
ApeKI GCWGC 3 cut(s) 20, 270, 539
Asp700I GAANNNNTTC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 190, 509
AsuHPI GGTGA 2 cut(s) 404, 489
AvaII GGWCC 2 cut(s) 190, 509
BalI TGGCCA 1 cut(s) 488
BanII GRGCYC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 3 cut(s) 32, 282, 526
BccI CCATC 1 cut(s) 423
BceAI ACGGC 1 cut(s) 121
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 3 cut(s) 485, 501, 513
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 2 cut(s) 65, 255
BglI GCCNNNNNGGC 1 cut(s) 385
BisI GCNGC 4 cut(s) 21, 151, 271, 540
BlsI GCNGC 4 cut(s) 22, 152, 272, 541
Bme1390I CCNGG 3 cut(s) 485, 501, 513
Bme18I GGWCC 2 cut(s) 190, 509
BmgT120I GGNCC 2 cut(s) 190, 509
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 3 cut(s) 485, 501, 513
BmsI GCATC 2 cut(s) 22, 353
BpmI CTGGAG 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 414
BsaJI CCNNGG 2 cut(s) 87, 543
BsaWI WCCGGW 1 cut(s) 192
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 506
Bse1I ACTGG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 3 cut(s) 485, 501, 513
BseDI CCNNGG 2 cut(s) 87, 543
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 88, 506
BseMII CTCAG 2 cut(s) 42, 463
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 477
BseXI GCAGC 3 cut(s) 32, 282, 526
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 289
BshFI GGCC 3 cut(s) 108, 488, 548
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 2 cut(s) 193, 380
BslFI GGGAC 2 cut(s) 342, 511
BslI CCNNNNNNNGG 2 cut(s) 88, 506
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 2 cut(s) 342, 511
BsnI GGCC 3 cut(s) 108, 488, 548
Bsp1286I GDGCHC 2 cut(s) 211, 300
Bsp143I GATC 5 cut(s) 162, 184, 349, 409, 452
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 2 cut(s) 150, 279
BspANI GGCC 3 cut(s) 108, 488, 548
BspCNI CTCAG 2 cut(s) 43, 462
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 1 cut(s) 170
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 87, 543
BssMI GATC 5 cut(s) 162, 184, 349, 409, 452
BssT1I CCWWGG 2 cut(s) 87, 543
Bst2UI CCWGG 3 cut(s) 485, 501, 513
Bst4CI ACNGT 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 51, 449
BstDSI CCRYGG 1 cut(s) 87
BstKTI GATC 5 cut(s) 165, 187, 352, 412, 455
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 5 cut(s) 162, 184, 349, 409, 452
BstMWI GCNNNNNNNGC 6 cut(s) 26, 267, 276, 385, 485, 545
BstNI CCWGG 3 cut(s) 485, 501, 513
BstSCI CCNGG 3 cut(s) 483, 499, 511
BstV1I GCAGC 3 cut(s) 32, 282, 526
BsuRI GGCC 3 cut(s) 108, 488, 548
BtgI CCRYGG 1 cut(s) 87
CaiI CAGNNNCTG 1 cut(s) 276
CciI TCATGA 1 cut(s) 418
Cfr13I GGNCC 2 cut(s) 190, 509
CseI GACGC 1 cut(s) 262
CviAII CATG 4 cut(s) 69, 88, 220, 419
DdeI CTNAG 2 cut(s) 51, 449
DpnI GATC 5 cut(s) 164, 186, 351, 411, 454
DpnII GATC 5 cut(s) 162, 184, 349, 409, 452
EaeI YGGCCR 2 cut(s) 106, 486
Eco130I CCWWGG 2 cut(s) 87, 543
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 190, 509
Eco57I CTGAAG 1 cut(s) 9
EcoRII CCWGG 3 cut(s) 483, 499, 511
EcoT14I CCWWGG 2 cut(s) 87, 543
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 2 cut(s) 87, 543
FaeI CATG 4 cut(s) 72, 91, 223, 422
FaqI GGGAC 2 cut(s) 342, 511
FatI CATG 4 cut(s) 68, 87, 219, 418
FauI CCCGC 1 cut(s) 272
FauNDI CATATG 1 cut(s) 441
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 4 cut(s) 21, 151, 271, 540
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 4 cut(s) 21, 151, 271, 540
FspBI CTAG 2 cut(s) 65, 255
GluI GCNGC 4 cut(s) 21, 151, 271, 540
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 3 cut(s) 108, 488, 548
HapII CCGG 2 cut(s) 193, 380
HgaI GACGC 1 cut(s) 262
Hin1II CATG 4 cut(s) 72, 91, 223, 422
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 3 cut(s) 55, 371, 494
HpaII CCGG 2 cut(s) 193, 380
HphI GGTGA 2 cut(s) 404, 489
Hpy166II GTNNAC 3 cut(s) 115, 334, 509
Hpy188I TCNGA 1 cut(s) 452
Hpy188III TCNNGA 4 cut(s) 188, 212, 250, 419
Hpy8I GTNNAC 3 cut(s) 115, 334, 509
Hpy99I CGWCG 2 cut(s) 107, 206
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 2 cut(s) 233, 330
HpyCH4V TGCA 4 cut(s) 13, 134, 270, 479
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 267, 276, 385, 485, 545
HpyF3I CTNAG 2 cut(s) 51, 449
HpySE526I ACGT 2 cut(s) 233, 330
Hsp92II CATG 4 cut(s) 72, 91, 223, 422
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 5 cut(s) 162, 184, 349, 409, 452
LmnI GCTCC 2 cut(s) 72, 258
Lsp1109I GCAGC 3 cut(s) 32, 282, 526
LweI GCATC 2 cut(s) 22, 353
MaeI CTAG 2 cut(s) 65, 255
MaeII ACGT 2 cut(s) 233, 330
MalI GATC 5 cut(s) 164, 186, 351, 411, 454
MboI GATC 5 cut(s) 162, 184, 349, 409, 452
MboII GAAGA 2 cut(s) 70, 169
MhlI GDGCHC 2 cut(s) 211, 300
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 1 cut(s) 550
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 380
MnlI CCTC 3 cut(s) 455, 458, 497
Mox20I TGGCCA 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 488
MslI CAYNNNNRTG 2 cut(s) 204, 417
Msp20I TGGCCA 1 cut(s) 488
MspI CCGG 2 cut(s) 193, 380
MspR9I CCNGG 3 cut(s) 485, 501, 513
MvaI CCWGG 3 cut(s) 485, 501, 513
MwoI GCNNNNNNNGC 6 cut(s) 26, 267, 276, 385, 485, 545
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 441
NdeII GATC 5 cut(s) 162, 184, 349, 409, 452
NlaIII CATG 4 cut(s) 72, 91, 223, 422
NlaIV GGNNCC 1 cut(s) 197
OliI CACNNNNGTG 1 cut(s) 204
PagI TCATGA 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 55, 494
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 4 cut(s) 22, 152, 272, 541
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp6I CCWGG 3 cut(s) 483, 499, 511
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 3 cut(s) 483, 499, 511
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 2 cut(s) 190, 509
PstNI CAGNNNCTG 1 cut(s) 276
RseI CAYNNNNRTG 2 cut(s) 204, 417
SatI GCNGC 4 cut(s) 21, 151, 271, 540
Sau3AI GATC 5 cut(s) 162, 184, 349, 409, 452
Sau96I GGNCC 2 cut(s) 190, 509
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 3 cut(s) 485, 501, 513
SduI GDGCHC 2 cut(s) 211, 300
SfaNI GCATC 2 cut(s) 22, 353
SinI GGWCC 2 cut(s) 190, 509
SmiMI CAYNNNNRTG 2 cut(s) 204, 417
Sse9I AATT 1 cut(s) 550
SsiI CCGC 2 cut(s) 150, 279
SspMI CTAG 2 cut(s) 65, 255
StyD4I CCNGG 3 cut(s) 483, 499, 511
StyI CCWWGG 2 cut(s) 87, 543
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 2 cut(s) 236, 333
TaqI TCGA 2 cut(s) 213, 374
TasI AATT 1 cut(s) 550
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 55, 494
TseI GCWGC 3 cut(s) 20, 270, 539
TspDTI ATGAA 1 cut(s) 208
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 190, 509
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 2 cut(s) 65, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.