Rorug05G0213400

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005123
Physical Location & Seq
Reverse (-)
21901636 .. 21902127
492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0213400.1

Sequence Viewer

Length: 492 bp
ATGGATATCCATGAGGATGGTAATGAAATATTACATGACAAGCATCATCTTCTTCCGGGCTACAGGTTCTGCCCCTTGGAGGAGGAACTAATCCTCCGCTATCTATGTCCCAAGGTCAAAGGAGAAGAGGTACCCGGTGAAGACTATTTGATTTTCAACTCCAATTTGTACGGTGAACAAGAGCCTTGGGACATATGGGACATGTACAAAGAAAGACGAAAGAACGACTTGAGGTTCAACAAAGATCTCTACTTCTTCACTCAACGAAAGAAGATGACTGTGAATGCCTCCCGGAAGGGCCGATCTATCGGAAGCGGCACCTGGAAAGGGGGGGACGCTGCCAAGAACGTATACGCAAGTAACATTGTTGTAGGTTACAGGAAAAGATTTACTTATGAGAAAAAGGGGTCCGAGCAACATGGCAGATGGATCATGCTCGAGTTCGAACTTGATCACTCGCAAGTAGGCATGCTTGACCAACAAGTAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

163

Amino Acids

19.3

Weight (kDa)

6.6

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 19 - 150 6.7e-25 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 130
AccB1I GGYRCC 2 cut(s) 130, 317
AccI GTMKAC 1 cut(s) 351
AciI CCGC 2 cut(s) 97, 315
AclWI GGATC 1 cut(s) 437
AfaI GTAC 3 cut(s) 132, 170, 206
AfiI CCNNNNNNNGG 2 cut(s) 79, 327
AflIII ACRYGT 1 cut(s) 201
AgsI TTSAA 2 cut(s) 157, 238
AjnI CCWGG 1 cut(s) 320
AloI GAACNNNNNNTCC 2 cut(s) 78, 110
AlwI GGATC 1 cut(s) 437
AlwNI CAGNNNCTG 1 cut(s) 69
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 1 cut(s) 298
ApeKI GCWGC 1 cut(s) 338
Asp718I GGTACC 1 cut(s) 130
AspS9I GGNCC 2 cut(s) 298, 408
AsuC2I CCSGG 3 cut(s) 57, 135, 292
AsuHPI GGTGA 2 cut(s) 149, 185
AsuII TTCGAA 1 cut(s) 444
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 1 cut(s) 408
BanI GGYRCC 2 cut(s) 130, 317
BbsI GAAGAC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 325
BccI CCATC 2 cut(s) 11, 420
BciT130I CCWGG 1 cut(s) 322
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 3 cut(s) 57, 135, 292
BfmI CTRYAG 1 cut(s) 61
BglII AGATCT 1 cut(s) 244
BisI GCNGC 2 cut(s) 316, 339
BlsI GCNGC 2 cut(s) 317, 340
Bme1390I CCNGG 4 cut(s) 57, 135, 292, 322
Bme18I GGWCC 1 cut(s) 408
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 2 cut(s) 298, 408
BmiI GGNNCC 3 cut(s) 132, 319, 409
BmrFI CCNGG 4 cut(s) 57, 135, 292, 322
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 250
BpuMI CCSGG 3 cut(s) 57, 135, 292
BsaJI CCNNGG 3 cut(s) 75, 111, 185
BsaXI ACNNNNNCTCC 4 cut(s) 78, 108, 114, 144
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 327
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 3 cut(s) 75, 111, 185
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 79, 327
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 325
BshFI GGCC 1 cut(s) 300
BshNI GGYRCC 2 cut(s) 130, 317
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 3 cut(s) 56, 135, 292
BslFI GGGAC 4 cut(s) 93, 203, 212, 347
BslI CCNNNNNNNGG 2 cut(s) 79, 327
BsmFI GGGAC 4 cut(s) 93, 203, 212, 347
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 1 cut(s) 300
BsoBI CYCGRG 1 cut(s) 437
Bsp119I TTCGAA 1 cut(s) 444
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 4 cut(s) 244, 302, 429, 451
BspACI CCGC 2 cut(s) 97, 315
BspANI GGCC 1 cut(s) 300
BspLI GGNNCC 3 cut(s) 132, 319, 409
BspPI GGATC 1 cut(s) 437
BspT104I TTCGAA 1 cut(s) 444
BspT107I GGYRCC 2 cut(s) 130, 317
BsrGI TGTACA 1 cut(s) 204
BssECI CCNNGG 3 cut(s) 75, 111, 185
BssMI GATC 4 cut(s) 244, 302, 429, 451
BssNAI GTATAC 1 cut(s) 352
BssT1I CCWWGG 3 cut(s) 75, 111, 185
Bst1107I GTATAC 1 cut(s) 352
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 2 cut(s) 173, 280
Bst6I CTCTTC 1 cut(s) 120
BstAUI TGTACA 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 470
BstF5I GGATG 1 cut(s) 22
BstKTI GATC 4 cut(s) 247, 305, 432, 454
BstMBI GATC 4 cut(s) 244, 302, 429, 451
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 2 cut(s) 205, 472
BstSCI CCNGG 4 cut(s) 55, 133, 290, 320
BstSFI CTRYAG 1 cut(s) 61
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 244
BstXI CCANNNNNNTGG 1 cut(s) 17
BstYI RGATCY 1 cut(s) 244
BstZ17I GTATAC 1 cut(s) 352
BsuRI GGCC 1 cut(s) 300
BtsCI GGATG 1 cut(s) 22
Cac8I GCNNGC 1 cut(s) 470
CaiI CAGNNNCTG 1 cut(s) 69
Cfr13I GGNCC 2 cut(s) 298, 408
CseI GACGC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 131, 169, 205
CviAII CATG 6 cut(s) 11, 35, 202, 419, 433, 469
CviJI RGCY 3 cut(s) 60, 184, 300
CviKI_1 RGCY 3 cut(s) 60, 184, 300
CviQI GTAC 3 cut(s) 131, 169, 205
DpnI GATC 4 cut(s) 246, 304, 431, 453
DpnII GATC 4 cut(s) 244, 302, 429, 451
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 3 cut(s) 75, 111, 185
Eco32I GATATC 1 cut(s) 7
Eco47I GGWCC 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 320
EcoRV GATATC 1 cut(s) 7
EcoT14I CCWWGG 3 cut(s) 75, 111, 185
ErhI CCWWGG 3 cut(s) 75, 111, 185
FaeI CATG 6 cut(s) 14, 38, 205, 422, 436, 472
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 376, 408
FaqI GGGAC 4 cut(s) 93, 203, 212, 347
FatI CATG 6 cut(s) 10, 34, 201, 418, 432, 468
FauNDI CATATG 1 cut(s) 194
FbaI TGATCA 1 cut(s) 451
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 2 cut(s) 316, 339
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 2 cut(s) 316, 339
GluI GCNGC 2 cut(s) 316, 339
HaeIII GGCC 1 cut(s) 300
HapII CCGG 3 cut(s) 56, 135, 292
HgaI GACGC 1 cut(s) 344
Hin1II CATG 6 cut(s) 14, 38, 205, 422, 436, 472
HpaII CCGG 3 cut(s) 56, 135, 292
HphI GGTGA 2 cut(s) 149, 185
Hpy166II GTNNAC 2 cut(s) 176, 352
Hpy188I TCNGA 2 cut(s) 311, 412
Hpy8I GTNNAC 2 cut(s) 176, 352
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 2 cut(s) 173, 280
HpyCH4IV ACGT 1 cut(s) 348
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 6 cut(s) 14, 38, 205, 422, 436, 472
KpnI GGTACC 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 4 cut(s) 244, 302, 429, 451
LpnPI CCDG 7 cut(s) 49, 69, 148, 305, 307, 334, 364
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 52
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 359, 374
MalI GATC 4 cut(s) 246, 304, 431, 453
MboI GATC 4 cut(s) 244, 302, 429, 451
MboII GAAGA 6 cut(s) 41, 44, 137, 152, 247, 283
MflI RGATCY 1 cut(s) 244
MluCI AATT 1 cut(s) 163
MnlI CCTC 7 cut(s) 7, 73, 76, 104, 121, 225, 298
MseI TTAA 1 cut(s) 490
MslI CAYNNNNRTG 1 cut(s) 15
MspI CCGG 3 cut(s) 56, 135, 292
MspR9I CCNGG 4 cut(s) 57, 135, 292, 322
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 322
NciI CCSGG 3 cut(s) 57, 135, 292
NdeI CATATG 1 cut(s) 194
NdeII GATC 4 cut(s) 244, 302, 429, 451
NlaIII CATG 6 cut(s) 14, 38, 205, 422, 436, 472
NlaIV GGNNCC 3 cut(s) 132, 319, 409
NspI RCATGY 2 cut(s) 205, 472
NspV TTCGAA 1 cut(s) 444
PaeI GCATGC 1 cut(s) 472
PaeR7I CTCGAG 1 cut(s) 437
PciI ACATGT 1 cut(s) 201
PctI GAATGC 1 cut(s) 289
PfoI TCCNGGA 1 cut(s) 290
PkrI GCNGC 2 cut(s) 317, 340
PscI ACATGT 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 3 cut(s) 132, 319, 409
PspPI GGNCC 2 cut(s) 298, 408
PspXI VCTCGAGB 1 cut(s) 437
PstNI CAGNNNCTG 1 cut(s) 69
PsuI RGATCY 1 cut(s) 244
RsaI GTAC 3 cut(s) 132, 170, 206
RsaNI GTAC 3 cut(s) 131, 169, 205
RseI CAYNNNNRTG 1 cut(s) 15
SaqAI TTAA 1 cut(s) 490
SatI GCNGC 2 cut(s) 316, 339
Sau3AI GATC 4 cut(s) 244, 302, 429, 451
Sau96I GGNCC 2 cut(s) 298, 408
ScrFI CCNGG 4 cut(s) 57, 135, 292, 322
SetI ASST 7 cut(s) 68, 117, 132, 236, 323, 351, 376
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 1 cut(s) 61
Sfr274I CTCGAG 1 cut(s) 437
SfuI TTCGAA 1 cut(s) 444
SinI GGWCC 1 cut(s) 408
SlaI CTCGAG 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 15
SmlI CTYRAG 2 cut(s) 229, 437
SmoI CTYRAG 2 cut(s) 229, 437
SphI GCATGC 1 cut(s) 472
Sse9I AATT 1 cut(s) 163
SsiI CCGC 2 cut(s) 97, 315
SspI AATATT 1 cut(s) 30
StyD4I CCNGG 4 cut(s) 55, 133, 290, 320
StyI CCWWGG 3 cut(s) 75, 111, 185
TaaI ACNGT 2 cut(s) 173, 280
TaiI ACGT 1 cut(s) 351
TaqI TCGA 2 cut(s) 438, 444
TasI AATT 1 cut(s) 163
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 318
Tru1I TTAA 1 cut(s) 490
Tru9I TTAA 1 cut(s) 490
TseI GCWGC 1 cut(s) 338
TspDTI ATGAA 1 cut(s) 39
VpaK11BI GGWCC 1 cut(s) 408
XceI RCATGY 2 cut(s) 205, 472
XhoI CTCGAG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.