Rorug05G0213600

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005123
Physical Location & Seq
Forward (+)
21912142 .. 21914265
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0213600.1

Sequence Viewer

Length: 2124 bp
ATGAAGACTGACGATTTCGACAATGATCAGGCTGGAACACAAATGGTTCAGGCTCAGAATGACTCGACCGAAAAGGCATTCAGAGACACGGAGGTGCTGCCATGGCACAAGCAAATAACTGTGAGGTCTATGGTCACAAGTTTTTTTCTTAGCATCATCTTCAACTTCATCGTGTGCAAGCTCAATCTCACCACTGGTGTAATACCATCTCTAAATGTCGCGGCGGGGCTGCTGGGATTCGCAATCATGAAAGGATACACTGCCTTACTTGACAAGTTAGGTCTGTTGAAGCAGCCTTTCACTCGTCAAGAAAACACAGTGATTCAAACCTGTGTTGTGGCCTCATCTGGGATAGCCTTTAGCAGCGGCATGGGGAGTTATTTACTAGGGATGAGCCCTCTAGTAGCTGCTCAGGGAGATGCAGGCAACACTCTAATCAACATCAAGAGGCTTTCGATTCCGTGGATGACAGGATATCTCTTCACAGTCAGCTTCGTTGGGCTGTTTTCAATGATACCCTTGAGAAAGATGATGATCATTGATAGAAAACTAACGTACCCAAGTGGAACAGCAACGGCGCACCTCATCAACAGCTTCCACACCCCCAAAGGAGCCAAGAAGGCAGCGAGACAGGTTGCTGTTCTGTTCAAGAGCTGCTGCGGCAGCTTTGCGTGGTCCTTTTTTCAGTGGTTTTTCGTGGCTTCGGATGGCTGTGGGTTTGCTAGCTTTCCAATTTTCGGTCTCAAAGCCTACAAGCAGAGGTTTTACTTCGACTTCTCAACCACATATATTGGTTGTGGAATGATCACCTCTTACATGGTGAACGTATCCATGCTCGTTGGAGCTGTGATATCATGGGGAATCATGTGGCCTTTGATTGAACAAAAGAAAGGTATCTGGTATGGTGATCATCTTTCTGCGAGTAGTCTTCATGGAATCCAAGGTTACAGGGTCTTTATTTCCATAGCAATGATCCTTGGTGATGGTCTATACCATGTTATGTACCATGCTGGTTATGCGGTTTTCATTATCATCAAAAGTCGGTTCAGCAATAAACCAGAAGAAAAGAAAGTAGAGTTGTCATCACCGGCACCTCTACCATTAACCAATATGGATGCTGGATTGCGCTCATCAGAAACTGAACTGAGTGAAAACTACGACGAGCAACGGAGAACTGAGTACTTCTTGAAAGACCAAATTCCGCGCCGGTTTGCTGTCTGTGGCTACGTTGCTCTTGCAGCCATATCGACTATTGTCCTTCCATTCGTGTTTCACCAACTAAAATGGTATTATGTTGTGGCAGCTTACGTCATCGCTCCTGTTCTGGCCTTCTGCAATGCCTATGGGTGCGGCCTCACAGATTGGTCCCTCGCTTCCAACTACGGCAAAATCGCAATCCTGATCTTCAGTGCTTGTGTTGGCCCAGAAAAAGGTGGTGTCATTGCTGGCCTCGCTTCATGTGGTGTAATGATGAGCATTGTCTCAACAGCCTCGGATCTCATGCAAGACTTCAAGACTGGATATCTAACTCTCGCGTCTCCTCGATCCATGTTCGTTAGCCAAGTTTTCGGGACAGCCATGGGGTGTGTCATGTCGCCTTTGACATTCTGGTTCTTCTACAAAGCTTACCGAGTTGGCGATCCAACGGGCTCGTATCCTGCACCCTATGGCCTAATGTACCGTGGAATAGCACTCCTCGGAGTGGAGGGCTTTGGCTCACTCCCCAAACACTGCGTTGCACTTGCAGTCTCCTTTTTCGCATTTGCCATTGCCATTAACGTAGTAACCGAGCTTTTGAAGCGTTACGAGACCAAGTACAAGATCTACAGATTTGTTCCAAATGCAATGGCCATGGCAGTCCCGTTCTATCTCGGCTCCTACTTCGTGATTGACATGTGCATAGGAAGCTTGATCCTTTTCGGCTGGAAGTTATGGAACAAACAACAGGCTGAGGATTTAGCACCGGCGGTTGCATCTGGTCTCATTTGTGGCGAGTCTATGTGGGGTGTTCCAGCTGCGATCCTATCTCTTGCCAATGTCAAAGCACCAATATGCATGAAGTTCCTCTCTTACTCAGACAATGATAGAGTTGATTCCTTTTTATCTTCATCAGGATCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

707

Amino Acids

77.78

Weight (kDa)

8.98

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 38 - 675 1.4e-152 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1090
AccII CGCG 3 cut(s) 221, 1204, 1535
AciI CCGC 8 cut(s) 221, 224, 366, 660, 1019, 1202, 1350, 1967
AclWI GGATC 7 cut(s) 967, 1503, 1539, 1634, 1906, 2014, 2122
AcoI YGGCCR 1 cut(s) 1848
AcsI RAATTY 1 cut(s) 1197
AcuI CTGAAG 1 cut(s) 1390
AfaI GTAC 5 cut(s) 557, 1004, 1181, 1679, 1817
AfiI CCNNNNNNNGG 4 cut(s) 348, 737, 1430, 1702
AflIII ACRYGT 1 cut(s) 1893
AgsI TTSAA 9 cut(s) 163, 289, 326, 510, 649, 881, 1189, 1513, 1798
AleI CACNNNNGTG 1 cut(s) 92
Alw26I GTCTC 8 cut(s) 78, 622, 746, 1486, 1542, 1753, 1802, 1985
AlwI GGATC 7 cut(s) 967, 1503, 1539, 1634, 1906, 2014, 2122
AlwNI CAGNNNCTG 1 cut(s) 1139
AoxI GGCC 8 cut(s) 339, 869, 1326, 1351, 1420, 1447, 1669, 1848
ApoI RAATTY 1 cut(s) 1197
AspLEI GCGC 3 cut(s) 580, 1128, 1206
AspS9I GGNCC 3 cut(s) 675, 1365, 1421
AsuHPI GGTGA 7 cut(s) 181, 799, 832, 917, 992, 1077, 1265
AsuNHI GCTAGC 1 cut(s) 722
AvaII GGWCC 2 cut(s) 675, 1365
BaeI ACNNNNGTAYC 2 cut(s) 539, 572
BalI TGGCCA 1 cut(s) 1850
BanI GGYRCC 1 cut(s) 1090
BanII GRGCYC 2 cut(s) 398, 1652
BbsI GAAGAC 2 cut(s) 11, 920
BbvCI CCTCAGC 1 cut(s) 1950
BccI CCATC 3 cut(s) 214, 701, 977
BceAI ACGGC 2 cut(s) 591, 1399
BcgI CGANNNNNNTGC 2 cut(s) 1227, 1261
BciVI GTATCC 3 cut(s) 248, 838, 1665
BclI TGATCA 4 cut(s) 25, 534, 804, 907
BcoDI GTCTC 8 cut(s) 78, 622, 746, 1486, 1542, 1753, 1802, 1985
BfaI CTAG 3 cut(s) 386, 401, 723
BfmI CTRYAG 1 cut(s) 1825
BfuI GTATCC 3 cut(s) 248, 838, 1665
BglI GCCNNNNNGGC 1 cut(s) 620
BglII AGATCT 1 cut(s) 1821
BmcAI AGTACT 1 cut(s) 1181
Bme18I GGWCC 2 cut(s) 675, 1365
BmgT120I GGNCC 3 cut(s) 675, 1365, 1421
BmiI GGNNCC 4 cut(s) 613, 1092, 1367, 1876
BmsI GCATC 4 cut(s) 162, 409, 1105, 1982
BmtI GCTAGC 1 cut(s) 726
BoxI GACNNNNGTC 1 cut(s) 1253
BpiI GAAGAC 2 cut(s) 11, 920
Bpu10I CCTNAGC 2 cut(s) 411, 1950
BpuEI CTTGAG 1 cut(s) 541
BsaBI GATNNNNATC 1 cut(s) 533
BsaI GGTCTC 3 cut(s) 746, 1802, 1985
BsaJI CCNNGG 9 cut(s) 101, 461, 940, 976, 1491, 1578, 1681, 1696, 1851
Bsc4I CCNNNNNNNGG 4 cut(s) 348, 737, 1430, 1702
Bse118I RCCGGY 3 cut(s) 1087, 1206, 1963
Bse1I ACTGG 2 cut(s) 199, 1522
Bse3DI GCAATG 5 cut(s) 975, 1342, 1440, 1767, 1851
Bse8I GATNNNNATC 1 cut(s) 533
BseDI CCNNGG 9 cut(s) 101, 461, 940, 976, 1491, 1578, 1681, 1696, 1851
BseGI GGATG 4 cut(s) 396, 471, 712, 1120
BseJI GATNNNNATC 1 cut(s) 533
BseLI CCNNNNNNNGG 4 cut(s) 348, 737, 1430, 1702
BseMI GCAATG 5 cut(s) 975, 1342, 1440, 1767, 1851
BseMII CTCAG 6 cut(s) 68, 425, 1136, 1167, 1941, 2088
BseNI ACTGG 2 cut(s) 199, 1522
BseRI GAGGAG 2 cut(s) 1530, 1685
BseYI CCCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 1644
Bsh1236I CGCG 3 cut(s) 221, 1204, 1535
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
BshNI GGYRCC 1 cut(s) 1090
BsiEI CGRYCG 1 cut(s) 69
BsiSI CCGG 3 cut(s) 1088, 1207, 1964
BslFI GGGAC 3 cut(s) 1351, 1585, 1844
BslI CCNNNNNNNGG 4 cut(s) 348, 737, 1430, 1702
BsmAI GTCTC 8 cut(s) 78, 622, 746, 1486, 1542, 1753, 1802, 1985
BsmBI CGTCTC 1 cut(s) 1542
BsmFI GGGAC 3 cut(s) 1351, 1585, 1844
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
Bso31I GGTCTC 3 cut(s) 746, 1802, 1985
Bsp1286I GDGCHC 2 cut(s) 398, 1652
Bsp19I CCATGG 3 cut(s) 101, 1578, 1851
BspACI CCGC 8 cut(s) 221, 224, 366, 660, 1019, 1202, 1350, 1967
BspANI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
BspCNI CTCAG 6 cut(s) 67, 424, 1137, 1168, 1942, 2087
BspFNI CGCG 3 cut(s) 221, 1204, 1535
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 613, 1092, 1367, 1876
BspOI GCTAGC 1 cut(s) 726
BspPI GGATC 7 cut(s) 967, 1503, 1539, 1634, 1906, 2014, 2122
BspT107I GGYRCC 1 cut(s) 1090
BspTNI GGTCTC 3 cut(s) 746, 1802, 1985
BsrDI GCAATG 5 cut(s) 975, 1342, 1440, 1767, 1851
BsrFI RCCGGY 3 cut(s) 1087, 1206, 1963
BsrI ACTGG 2 cut(s) 199, 1522
BssAI RCCGGY 3 cut(s) 1087, 1206, 1963
BssECI CCNNGG 9 cut(s) 101, 461, 940, 976, 1491, 1578, 1681, 1696, 1851
BssT1I CCWWGG 5 cut(s) 101, 940, 976, 1578, 1851
Bst4CI ACNGT 4 cut(s) 121, 319, 487, 1682
Bst6I CTCTTC 1 cut(s) 485
BstC8I GCNNGC 4 cut(s) 179, 424, 724, 1447
BstDEI CTNAG 8 cut(s) 54, 149, 411, 1145, 1176, 1950, 2074, 2118
BstDSI CCRYGG 5 cut(s) 101, 461, 1578, 1681, 1851
BstF5I GGATG 4 cut(s) 396, 471, 712, 1120
BstFNI CGCG 3 cut(s) 221, 1204, 1535
BstHHI GCGC 3 cut(s) 580, 1128, 1206
BstMAI GTCTC 8 cut(s) 78, 622, 746, 1486, 1542, 1753, 1802, 1985
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 9 cut(s) 103, 620, 660, 663, 1016, 1238, 1451, 1798, 1905
BstNSI RCATGY 1 cut(s) 1897
BstPAI GACNNNNGTC 1 cut(s) 1253
BstSFI CTRYAG 1 cut(s) 1825
BstUI CGCG 3 cut(s) 221, 1204, 1535
BstV2I GAAGAC 2 cut(s) 11, 920
BstX2I RGATCY 3 cut(s) 1495, 1821, 2114
BstYI RGATCY 3 cut(s) 1495, 1821, 2114
BsuI GTATCC 3 cut(s) 248, 838, 1665
BsuRI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
BtgI CCRYGG 5 cut(s) 101, 461, 1578, 1681, 1851
BtgZI GCGATG 1 cut(s) 1297
BtsCI GGATG 4 cut(s) 396, 471, 712, 1120
BtsI GCAGTG 2 cut(s) 258, 1729
BtsIMutI CAGTG 6 cut(s) 192, 258, 324, 692, 1414, 1729
Cac8I GCNNGC 4 cut(s) 179, 424, 724, 1447
CaiI CAGNNNCTG 1 cut(s) 1139
CciI TCATGA 1 cut(s) 246
CfoI GCGC 3 cut(s) 580, 1128, 1206
Cfr10I RCCGGY 3 cut(s) 1087, 1206, 1963
Cfr13I GGNCC 3 cut(s) 675, 1365, 1421
CseI GACGC 1 cut(s) 1524
Csp6I GTAC 5 cut(s) 556, 1003, 1180, 1678, 1816
CspCI CAANNNNNGTGG 2 cut(s) 772, 807
CviQI GTAC 5 cut(s) 556, 1003, 1180, 1678, 1816
DdeI CTNAG 8 cut(s) 54, 149, 411, 1145, 1176, 1950, 2074, 2118
EaeI YGGCCR 1 cut(s) 1848
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
Eco130I CCWWGG 5 cut(s) 101, 940, 976, 1578, 1851
Eco24I GRGCYC 2 cut(s) 398, 1652
Eco31I GGTCTC 3 cut(s) 746, 1802, 1985
Eco32I GATATC 3 cut(s) 476, 852, 1523
Eco47I GGWCC 2 cut(s) 675, 1365
Eco57I CTGAAG 1 cut(s) 1390
EcoRV GATATC 3 cut(s) 476, 852, 1523
EcoT14I CCWWGG 5 cut(s) 101, 940, 976, 1578, 1851
EcoT22I ATGCAT 1 cut(s) 2057
EcoT38I GRGCYC 2 cut(s) 398, 1652
ErhI CCWWGG 5 cut(s) 101, 940, 976, 1578, 1851
Esp3I CGTCTC 1 cut(s) 1542
FaqI GGGAC 3 cut(s) 1351, 1585, 1844
FauI CCCGC 1 cut(s) 217
FbaI TGATCA 4 cut(s) 25, 534, 804, 907
FokI GGATG 4 cut(s) 403, 478, 719, 1127
FriOI GRGCYC 2 cut(s) 398, 1652
FspBI CTAG 3 cut(s) 386, 401, 723
GlaI GCGC 3 cut(s) 579, 1127, 1205
GsaI CCCAGC 1 cut(s) 236
HaeIII GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
HapII CCGG 3 cut(s) 1088, 1207, 1964
HgaI GACGC 1 cut(s) 1524
HhaI GCGC 3 cut(s) 580, 1128, 1206
Hin6I GCGC 3 cut(s) 578, 1126, 1204
HinP1I GCGC 3 cut(s) 578, 1126, 1204
HindIII AAGCTT 2 cut(s) 1623, 1906
HinfI GANTC 8 cut(s) 62, 237, 322, 457, 861, 936, 1994, 2093
HpaII CCGG 3 cut(s) 1088, 1207, 1964
HphI GGTGA 7 cut(s) 181, 799, 832, 917, 992, 1077, 1265
Hpy166II GTNNAC 1 cut(s) 823
Hpy188I TCNGA 7 cut(s) 57, 83, 706, 1135, 1495, 1700, 2077
Hpy8I GTNNAC 1 cut(s) 823
Hpy99I CGWCG 1 cut(s) 1163
HpyAV CCTTC 3 cut(s) 613, 1268, 1339
HpyCH4III ACNGT 4 cut(s) 121, 319, 487, 1682
HpyCH4IV ACGT 5 cut(s) 554, 825, 1227, 1308, 1779
HpyF10VI GCNNNNNNNGC 9 cut(s) 103, 620, 660, 663, 1016, 1238, 1451, 1798, 1905
HpyF3I CTNAG 8 cut(s) 54, 149, 411, 1145, 1176, 1950, 2074, 2118
HpySE526I ACGT 5 cut(s) 554, 825, 1227, 1308, 1779
HspAI GCGC 3 cut(s) 578, 1126, 1204
Ksp22I TGATCA 4 cut(s) 25, 534, 804, 907
LmnI GCTCC 4 cut(s) 611, 842, 1321, 1880
LweI GCATC 4 cut(s) 162, 409, 1105, 1982
MaeI CTAG 3 cut(s) 386, 401, 723
MaeII ACGT 5 cut(s) 554, 825, 1227, 1308, 1779
MaeIII GTNAC 4 cut(s) 133, 944, 1783, 1802
MboII GAAGA 8 cut(s) 16, 151, 472, 920, 1073, 1396, 1606, 2097
MflI RGATCY 3 cut(s) 1495, 1821, 2114
MhlI GDGCHC 2 cut(s) 398, 1652
MlsI TGGCCA 1 cut(s) 1850
MluCI AATT 2 cut(s) 732, 1197
MluNI TGGCCA 1 cut(s) 1850
MlyI GAGTC 2 cut(s) 56, 2003
MmeI TCCRAC 3 cut(s) 820, 1401, 1667
Mox20I TGGCCA 1 cut(s) 1850
Mph1103I ATGCAT 1 cut(s) 2057
MscI TGGCCA 1 cut(s) 1850
MseI TTAA 2 cut(s) 1103, 1776
MslI CAYNNNNRTG 3 cut(s) 92, 968, 2050
Msp20I TGGCCA 1 cut(s) 1850
MspA1I CMGCKG 2 cut(s) 366, 2015
MspI CCGG 3 cut(s) 1088, 1207, 1964
Mva1269I GAATGC 1 cut(s) 77
MvnI CGCG 3 cut(s) 221, 1204, 1535
MwoI GCNNNNNNNGC 9 cut(s) 103, 620, 660, 663, 1016, 1238, 1451, 1798, 1905
NcoI CCATGG 3 cut(s) 101, 1578, 1851
NheI GCTAGC 1 cut(s) 722
NlaIV GGNNCC 4 cut(s) 613, 1092, 1367, 1876
NmeAIII GCCGAG 1 cut(s) 1851
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 2057
NspI RCATGY 1 cut(s) 1897
OliI CACNNNNGTG 1 cut(s) 92
PagI TCATGA 1 cut(s) 246
PciI ACATGT 1 cut(s) 1893
PcsI WCGNNNNNNNCGW 1 cut(s) 1785
PctI GAATGC 1 cut(s) 77
PfeI GAWTC 6 cut(s) 237, 322, 457, 861, 936, 2093
PleI GAGTC 2 cut(s) 56, 2002
PpsI GAGTC 2 cut(s) 56, 2002
PscI ACATGT 1 cut(s) 1893
PshAI GACNNNNGTC 1 cut(s) 1253
PspFI CCCAGC 1 cut(s) 232
PspN4I GGNNCC 4 cut(s) 613, 1092, 1367, 1876
PspPI GGNCC 3 cut(s) 675, 1365, 1421
PstNI CAGNNNCTG 1 cut(s) 1139
PsuI RGATCY 3 cut(s) 1495, 1821, 2114
PvuII CAGCTG 1 cut(s) 2015
RsaI GTAC 5 cut(s) 557, 1004, 1181, 1679, 1817
RsaNI GTAC 5 cut(s) 556, 1003, 1180, 1678, 1816
RseI CAYNNNNRTG 3 cut(s) 92, 968, 2050
SaqAI TTAA 2 cut(s) 1103, 1776
Sau96I GGNCC 3 cut(s) 675, 1365, 1421
ScaI AGTACT 1 cut(s) 1181
SchI GAGTC 2 cut(s) 56, 2003
SduI GDGCHC 2 cut(s) 398, 1652
SfaNI GCATC 4 cut(s) 162, 409, 1105, 1982
SfcI CTRYAG 1 cut(s) 1825
SgrAI CRCCGGYG 1 cut(s) 1963
SinI GGWCC 2 cut(s) 675, 1365
SmiMI CAYNNNNRTG 3 cut(s) 92, 968, 2050
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 2 cut(s) 732, 1197
SsiI CCGC 8 cut(s) 221, 224, 366, 660, 1019, 1202, 1350, 1967
SspMI CTAG 3 cut(s) 386, 401, 723
StyI CCWWGG 5 cut(s) 101, 940, 976, 1578, 1851
TaaI ACNGT 4 cut(s) 121, 319, 487, 1682
TaiI ACGT 5 cut(s) 557, 828, 1230, 1311, 1782
TaqI TCGA 6 cut(s) 18, 65, 455, 771, 1247, 1543
TaqII GACCGA 2 cut(s) 83, 728
TasI AATT 2 cut(s) 732, 1197
TatI WGTACW 2 cut(s) 1179, 1815
TauI GCSGC 4 cut(s) 224, 369, 663, 1353
TfiI GAWTC 6 cut(s) 237, 322, 457, 861, 936, 2093
Tru1I TTAA 2 cut(s) 1103, 1776
Tru9I TTAA 2 cut(s) 1103, 1776
TscAI CASTG 6 cut(s) 199, 265, 324, 692, 1414, 1736
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 8 cut(s) 17, 157, 263, 920, 1015, 1446, 2072, 2097
TspGWI ACGGA 3 cut(s) 104, 450, 1183
TspRI CASTG 6 cut(s) 199, 265, 324, 692, 1414, 1736
VpaK11BI GGWCC 2 cut(s) 675, 1365
XapI RAATTY 1 cut(s) 1197
XceI RCATGY 1 cut(s) 1897
XspI CTAG 3 cut(s) 386, 401, 723
ZrmI AGTACT 1 cut(s) 1181
Zsp2I ATGCAT 1 cut(s) 2057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.