Rorug05G0281100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0011546
Physical Location & Seq
Reverse (-)
32042217 .. 32131979
89763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0281100.1

Sequence Viewer

Length: 2943 bp
ATGAGCTTGATGGAGAATCAGAAGAGGAGGATCAGTATCATCCTTGTTCTTTTCTCATGGATTTTCATGGTGGCCGCCATGGCAGCTGAAAACACATCAACAATCCCAGTAAACGTGGGAGTTGTTGTAGACCTTGATGAACCGAGTGGGAAGATGTATTTGAGTTGCATAGAAATGGCGCTCTCAGACTTCTATGCTTCCCATGCTCAATACAAGACCAGGGTCGTTTTGAACACCAGTAACTCCCACGAAGATGTCGTTGGTGCAGCTGATGCAGCTTTGGATCTGATAACGAATGTAGAAGTGAAAGCAATCCTAGGACCAATGACATCAATGCAAGCAACTTTTGTTGTTAAACTTGGAGAAAAAGCTCATGTGCCCATCATATCATTTTCTGCAACAAGCCCTTCTCTTACTTCACTTCAGAGCTCCTATTTTTTCCAATTTGCACAAAATGACTCAACCCAAGTGAAAGCCATAAGTGGTATTGTTCAAAACTTTGGATGGAGGCGAGTTGTCCCCATTTACGTAGACAATATGTACGGGGAAGGAATCATGCCCTTTCTAATTGATGCCTTGCTCGAGGTTGATGCTCATGTCCCATATCGAAGTGTAATTTCACAATCAGCCACCGATGATCAAGTTGAGATAGAACTTTACAAGTTAATGACAATGCAGACTAGAGTCTTCATAGTGCATATGATGCCTAAACTTGCATCCCAGTTATTTGCCAAGGCGAAAGAGATTGGAATGATGAACAAGGGTTTTGTATGGATCACGACCAATGGGATTGGAGATCGTTTAAAGGCAATGGGATCTGTTATGAATTCGATGCAAGGAGTATTAAGTGTTGAAACTGCTGTTTTAGACACATTGAAGCTCAGAGAATTCAAAACAAGGTGGAAAAGACAATTCCAACAAGATTATCCAGAAATCATTGATGTTGAGCTTGATGCCTTTGGATATCGGGCTTATGATGCAGCTTTTGCAATAGCCATGGCAGTTGAAAAAGTTGGGAATGCAGGCTTTGGATTTCAAAAGGGAAATGCTTCATCTTTCAAATCTACAGATCTTGGAACTCTAAAGGTCTCTCAATATGGCTCAAACCTTTCTAATGCCTTATCTCTTACCAGTTTCAGAGGCATAGTTGGTGATTTTAGTCTTGTTGATAGGCAACTTCAACTGTCAACTTTTATGATTGTTAATGTGAATGGTAATGGAAAGACAAAACTCGGTTATTACAATTGGACACCGGAAAATGGAAGGTTAATGAAGCAGTTGAATTCAACAAACAGTTGTAATGTTTCAGGTTTCAAGTGTGATGCTCTTGGACCCATTATATGGCCGGGAGAGTCATCCTCCGTTCCCAAAGGATGGGAGATCCCAGAAAATGGTACGAGGTTAAGATTTGGAGTTCCAGTGAAGGATGGTTTTACAGAGTTTGTTAAAGTAACAACAGATTTGGGAACTAATACCACTGATGTCACCGGGTTCAGTATTGATGTGTTTAAAGCTGTAGTGGAACTGTTACCATATGCACTTCCTTATGAGTTGATTCCCTATGCATATCCTAATGGCACCAGCGCTGGCACATATAATGATTTAATCTATCAAGTATACCTTGGGAATTTTGACGGTGTGGTTGGAGATGCAACAATTAGAGCAAACAGAACATTGTATGTGGATTTTGCAATGCCATATACGGAGTCTGGTGTAGTGATGGTTGTGCCAATCATAGACAGCAGAACCAAAAATGCATGGGTTTTTTTGCAGCCTTTAACATGGAACCTTTGGATGACTACTTTGTGTTTTTTCATCTTTATTGGTTTTGTGATTTGGGTGCTTGAACACCGAATAAATGAAGATTTCCGTGGTCCTCCTTCACATCAAGTTGGCACAAGCTTTTGGTTCTCCTTTTCAACTATGGTTTTTTCACATAGGGAGAGAGTGGTGAGCAACCTGGGAAGATTTGTGATGATTATATGGGTATTTGTTGTGCTGATATTGACACAAAGCTACACTGCTAATCTAGCATCACTATTAACTGTCCAACAACTCCGACCAGCTGTCAACGATATAAATGATCTTCGAAGGAATGGTGATAACGTGGGCTACATGAGGGGTGCTTTTGTTTATGATCTCTTAAAAGAAGTAAAGTTTGATGATGCCAAGCTCAAGGCTTATGGATCTATGGAAGAAATAGATGACGCTCTTTCAAAAGGGAGTGCAAATGGTGGTATTGCTGCAATTGTTCATGAAACCCCCTACATGAAGCTACTCATTGCAAAATATTGTTCCAAGTATACTATGATTGGACCAATATTTAAAACCGATGGCTTTGGCTTCGCATTTCCAAAAAATTCCCCTCTTGTATCTGACATTACACAAGCAATCCTAAATTTGACGGATGATGGATTGATGACAAAGATTGAAGATAAATGGATCAAGAAAGATAGTAACTGTAAAGACTCTAATGGGAATTTTTCTAGCAATGCTCTTGGTCTTGAGAGCTTTTGGGGCCTTTTTTTAATTGCAGGGATAACGTCAATATTTGCTCTAATCATATTTGTAACTTCCTTCTTGTACGAGCATAAGCATGTCTTTATGTACCCTGATTCAGGCACCTCTAAGTCAAAAAGGATCAAAACCATGTTTGAGATCTTCAACGAAAAAAAACTCAGCTCTCATACATGTAGAAGCAGCCAACATTCAGACAGTGGTGTTAGTGCTCAAGATCATGAAGTGAAAGTATCACCAAATAACAACTGGCCAGAAAGTCCACAGAGCTATACCAACCACACCGATGCGACTTATGTATTTTCTCGGGAACAAGAAACACCGTCTCATGGTCAAGCATCTCCGGAAACCATTCCAACTATTGAGCTTGCCATCACTATTCAAGAAACCCATGAAACTCTTGATACAACTCGATCAAGAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

980

Amino Acids

109.17

Weight (kDa)

5.86

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 36 - 339 1.7e-21 Periplasmic binding protein
ANF_receptor PF01094 56 - 406 5e-73 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 487 - 576 3.3e-06 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 492 - 816 2.9e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 594 - 847 3.9e-42 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1577, 2621
AccB7I CCANNNNNTGG 3 cut(s) 1341, 1374, 1391
AccI GTMKAC 4 cut(s) 129, 531, 1617, 2303
AccIII TCCGGA 1 cut(s) 2860
AciI CCGC 1 cut(s) 75
AclWI GGATC 8 cut(s) 38, 291, 782, 823, 1375, 2194, 2450, 2648
AcoI YGGCCR 3 cut(s) 72, 1343, 2768
AcsI RAATTY 7 cut(s) 826, 887, 1282, 1627, 2359, 2398, 2479
AcuI CTGAAG 1 cut(s) 407
AfaI GTAC 4 cut(s) 542, 1396, 2585, 2609
AfeI AGCGCT 1 cut(s) 1585
AfiI CCNNNNNNNGG 6 cut(s) 1259, 1341, 1374, 1391, 2618, 2846
AflIII ACRYGT 1 cut(s) 2690
AhdI GACNNNNNGTC 1 cut(s) 2066
AjnI CCWGG 2 cut(s) 218, 1959
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
Alw21I GWGCWC 2 cut(s) 431, 2731
Alw26I GTCTC 2 cut(s) 1093, 2847
AlwI GGATC 8 cut(s) 38, 291, 782, 823, 1375, 2194, 2450, 2648
Ama87I CYCGRG 2 cut(s) 581, 2823
Aor13HI TCCGGA 1 cut(s) 2860
Aor51HI AGCGCT 1 cut(s) 1585
AoxI GGCC 4 cut(s) 72, 1343, 2518, 2768
ApeKI GCWGC 7 cut(s) 83, 266, 275, 980, 1771, 2243, 2700
ApoI RAATTY 7 cut(s) 826, 887, 1282, 1627, 2359, 2398, 2479
Asp700I GAANNNNTTC 2 cut(s) 1048, 2868
AspA2I CCTAGG 1 cut(s) 316
AspLEI GCGC 2 cut(s) 181, 1586
AspS9I GGNCC 5 cut(s) 320, 1331, 1874, 2315, 2518
AsuC2I CCSGG 2 cut(s) 1347, 1489
AsuHPI GGTGA 5 cut(s) 1163, 1477, 1963, 2111, 2745
AsuII TTCGAA 1 cut(s) 2089
AvaI CYCGRG 2 cut(s) 581, 2823
AvaII GGWCC 4 cut(s) 320, 1331, 1874, 2315
AvrII CCTAGG 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 381
BalI TGGCCA 1 cut(s) 2770
BanI GGYRCC 2 cut(s) 1577, 2621
BanII GRGCYC 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 679
Bbv12I GWGCWC 2 cut(s) 431, 2731
BbvI GCAGC 7 cut(s) 95, 278, 287, 992, 1783, 2230, 2712
BccI CCATC 9 cut(s) 4, 389, 498, 1368, 1421, 1714, 2327, 2405, 2897
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 2 cut(s) 220, 1961
BclI TGATCA 1 cut(s) 637
BcnI CCSGG 2 cut(s) 1347, 1489
BcoDI GTCTC 2 cut(s) 1093, 2847
BfaI CTAG 4 cut(s) 317, 681, 2030, 2487
BfmI CTRYAG 2 cut(s) 1065, 1515
BfoI RGCGCY 2 cut(s) 182, 1587
BglI GCCNNNNNGGC 1 cut(s) 80
BglII AGATCT 2 cut(s) 1069, 2658
BisI GCNGC 8 cut(s) 75, 84, 267, 276, 981, 1772, 2244, 2701
BlnI CCTAGG 1 cut(s) 316
BlsI GCNGC 8 cut(s) 76, 85, 268, 277, 982, 1773, 2245, 2702
Bme1390I CCNGG 4 cut(s) 220, 1347, 1489, 1961
Bme18I GGWCC 4 cut(s) 320, 1331, 1874, 2315
BmeRI GACNNNNNGTC 1 cut(s) 2066
BmeT110I CYCGRG 2 cut(s) 581, 2823
BmgT120I GGNCC 5 cut(s) 320, 1331, 1874, 2315, 2518
BmiI GGNNCC 5 cut(s) 1333, 1579, 1787, 2519, 2623
BmrFI CCNGG 4 cut(s) 220, 1347, 1489, 1961
BmrI ACTGGG 2 cut(s) 101, 715
BmuI ACTGGG 2 cut(s) 101, 715
BoxI GACNNNNGTC 2 cut(s) 221, 683
BpiI GAAGAC 1 cut(s) 679
BplI GAGNNNNNCTC 2 cut(s) 1343, 1375
Bpu14I TTCGAA 1 cut(s) 2089
BpuEI CTTGAG 3 cut(s) 2159, 2525, 2715
BpuMI CCSGG 2 cut(s) 1347, 1489
BsaAI YACGTR 1 cut(s) 529
BsaBI GATNNNNATC 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 1093
BsaJI CCNNGG 8 cut(s) 78, 219, 316, 732, 996, 1621, 1870, 1960
BsaWI WCCGGW 2 cut(s) 1252, 2860
BsaXI ACNNNNNCTCC 6 cut(s) 19, 49, 111, 141, 1404, 1434
Bsc4I CCNNNNNNNGG 6 cut(s) 1259, 1341, 1374, 1391, 2618, 2846
Bse1I ACTGG 6 cut(s) 107, 237, 721, 1131, 1418, 2771
Bse3DI GCAATG 4 cut(s) 816, 1698, 2280, 2497
Bse8I GATNNNNATC 1 cut(s) 35
BseAI TCCGGA 1 cut(s) 2860
BseBI CCWGG 2 cut(s) 220, 1961
BseDI CCNNGG 8 cut(s) 78, 219, 316, 732, 996, 1621, 1870, 1960
BseGI GGATG 8 cut(s) 39, 509, 716, 1355, 1379, 1432, 1800, 2413
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 6 cut(s) 1259, 1341, 1374, 1391, 2618, 2846
BseMI GCAATG 4 cut(s) 816, 1698, 2280, 2497
BseMII CTCAG 3 cut(s) 198, 895, 2692
BseNI ACTGG 6 cut(s) 107, 237, 721, 1131, 1418, 2771
BseRI GAGGAG 1 cut(s) 40
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 7 cut(s) 95, 278, 287, 992, 1783, 2230, 2712
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 4 cut(s) 74, 1345, 2520, 2770
BshNI GGYRCC 2 cut(s) 1577, 2621
BsiHKAI GWGCWC 2 cut(s) 431, 2731
BsiHKCI CYCGRG 2 cut(s) 581, 2823
BsiSI CCGG 4 cut(s) 1253, 1346, 1488, 2861
BslFI GGGAC 2 cut(s) 503, 584
BslI CCNNNNNNNGG 6 cut(s) 1259, 1341, 1374, 1391, 2618, 2846
BsmAI GTCTC 2 cut(s) 1093, 2847
BsmBI CGTCTC 1 cut(s) 2847
BsmFI GGGAC 2 cut(s) 503, 584
BsmI GAATGC 1 cut(s) 1024
BsnI GGCC 4 cut(s) 74, 1345, 2520, 2770
Bso31I GGTCTC 1 cut(s) 1093
BsoBI CYCGRG 2 cut(s) 581, 2823
Bsp119I TTCGAA 1 cut(s) 2089
Bsp1286I GDGCHC 3 cut(s) 381, 431, 2731
Bsp13I TCCGGA 1 cut(s) 2860
Bsp19I CCATGG 2 cut(s) 78, 996
BspACI CCGC 1 cut(s) 75
BspANI GGCC 4 cut(s) 74, 1345, 2520, 2770
BspCNI CTCAG 3 cut(s) 197, 894, 2691
BspEI TCCGGA 1 cut(s) 2860
BspHI TCATGA 2 cut(s) 2254, 2737
BspLI GGNNCC 5 cut(s) 1333, 1579, 1787, 2519, 2623
BspPI GGATC 8 cut(s) 38, 291, 782, 823, 1375, 2194, 2450, 2648
BspT104I TTCGAA 1 cut(s) 2089
BspT107I GGYRCC 2 cut(s) 1577, 2621
BspTNI GGTCTC 1 cut(s) 1093
BsrDI GCAATG 4 cut(s) 816, 1698, 2280, 2497
BsrI ACTGG 6 cut(s) 107, 237, 721, 1131, 1418, 2771
BssECI CCNNGG 8 cut(s) 78, 219, 316, 732, 996, 1621, 1870, 1960
BssNAI GTATAC 2 cut(s) 1618, 2304
BssT1I CCWWGG 5 cut(s) 78, 316, 732, 996, 1621
Bst1107I GTATAC 2 cut(s) 1618, 2304
Bst2UI CCWGG 2 cut(s) 220, 1961
Bst4CI ACNGT 8 cut(s) 1185, 1295, 1527, 1637, 2047, 2462, 2717, 2841
Bst6I CTCTTC 1 cut(s) 17
BstAPI GCANNNNNTGC 3 cut(s) 272, 703, 986
BstBAI YACGTR 1 cut(s) 529
BstBI TTCGAA 1 cut(s) 2089
BstC8I GCNNGC 4 cut(s) 339, 1024, 1588, 2886
BstDEI CTNAG 4 cut(s) 184, 881, 2628, 2678
BstDSI CCRYGG 3 cut(s) 78, 996, 1870
BstENI CCTNNNNNAGG 1 cut(s) 2616
BstF5I GGATG 8 cut(s) 39, 509, 716, 1355, 1379, 1432, 1800, 2413
BstH2I RGCGCY 2 cut(s) 182, 1587
BstHHI GCGC 2 cut(s) 181, 1586
BstMAI GTCTC 2 cut(s) 1093, 2847
BstNI CCWGG 2 cut(s) 220, 1961
BstNSI RCATGY 2 cut(s) 2600, 2694
BstPAI GACNNNNGTC 2 cut(s) 221, 683
BstSCI CCNGG 4 cut(s) 218, 1345, 1487, 1959
BstSFI CTRYAG 2 cut(s) 1065, 1515
BstSLI GKGCMC 1 cut(s) 381
BstSNI TACGTA 1 cut(s) 529
BstV1I GCAGC 7 cut(s) 95, 278, 287, 992, 1783, 2230, 2712
BstV2I GAAGAC 1 cut(s) 679
BstX2I RGATCY 6 cut(s) 283, 815, 1069, 1380, 2186, 2658
BstYI RGATCY 6 cut(s) 283, 815, 1069, 1380, 2186, 2658
BstZ17I GTATAC 2 cut(s) 1618, 2304
BsuRI GGCC 4 cut(s) 74, 1345, 2520, 2770
BtgI CCRYGG 3 cut(s) 78, 996, 1870
BtsCI GGATG 8 cut(s) 39, 509, 716, 1355, 1379, 1432, 1800, 2413
BtsI GCAGTG 1 cut(s) 2019
BtsIMutI CAGTG 4 cut(s) 1425, 1476, 2019, 2722
Cac8I GCNNGC 4 cut(s) 339, 1024, 1588, 2886
CciI TCATGA 2 cut(s) 2254, 2737
CfoI GCGC 2 cut(s) 181, 1586
Cfr13I GGNCC 5 cut(s) 320, 1331, 1874, 2315, 2518
CseI GACGC 1 cut(s) 2216
Csp6I GTAC 4 cut(s) 541, 1395, 2584, 2608
CviQI GTAC 4 cut(s) 541, 1395, 2584, 2608
DdeI CTNAG 4 cut(s) 184, 881, 2628, 2678
DraI TTTAAA 3 cut(s) 804, 1510, 2326
DriI GACNNNNNGTC 1 cut(s) 2066
EaeI YGGCCR 3 cut(s) 72, 1343, 2768
Eam1104I CTCTTC 1 cut(s) 17
Eam1105I GACNNNNNGTC 1 cut(s) 2066
EarI CTCTTC 1 cut(s) 17
Ecl136II GAGCTC 1 cut(s) 429
Eco105I TACGTA 1 cut(s) 529
Eco130I CCWWGG 5 cut(s) 78, 316, 732, 996, 1621
Eco24I GRGCYC 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 1093
Eco32I GATATC 1 cut(s) 965
Eco47I GGWCC 4 cut(s) 320, 1331, 1874, 2315
Eco47III AGCGCT 1 cut(s) 1585
Eco53kI GAGCTC 1 cut(s) 429
Eco57I CTGAAG 1 cut(s) 407
Eco88I CYCGRG 2 cut(s) 581, 2823
EcoICRI GAGCTC 1 cut(s) 429
EcoNI CCTNNNNNAGG 1 cut(s) 2616
EcoO109I RGGNCCY 1 cut(s) 2518
EcoRI GAATTC 3 cut(s) 826, 887, 1282
EcoRII CCWGG 2 cut(s) 218, 1959
EcoRV GATATC 1 cut(s) 965
EcoT14I CCWWGG 5 cut(s) 78, 316, 732, 996, 1621
EcoT22I ATGCAT 2 cut(s) 1567, 1759
EcoT38I GRGCYC 1 cut(s) 431
ErhI CCWWGG 5 cut(s) 78, 316, 732, 996, 1621
Esp3I CGTCTC 1 cut(s) 2847
FalI AAGNNNNNCTT 4 cut(s) 1605, 1637, 2585, 2617
FaqI GGGAC 2 cut(s) 503, 584
FauNDI CATATG 2 cut(s) 699, 1534
FbaI TGATCA 1 cut(s) 637
FblI GTMKAC 4 cut(s) 129, 531, 1617, 2303
Fnu4HI GCNGC 8 cut(s) 75, 84, 267, 276, 981, 1772, 2244, 2701
FokI GGATG 8 cut(s) 26, 516, 703, 1342, 1386, 1439, 1807, 2420
FriOI GRGCYC 1 cut(s) 431
Fsp4HI GCNGC 8 cut(s) 75, 84, 267, 276, 981, 1772, 2244, 2701
FspBI CTAG 4 cut(s) 317, 681, 2030, 2487
GlaI GCGC 2 cut(s) 180, 1585
GluI GCNGC 8 cut(s) 75, 84, 267, 276, 981, 1772, 2244, 2701
HaeII RGCGCY 2 cut(s) 182, 1587
HaeIII GGCC 4 cut(s) 74, 1345, 2520, 2770
HapII CCGG 4 cut(s) 1253, 1346, 1488, 2861
HgaI GACGC 1 cut(s) 2216
HhaI GCGC 2 cut(s) 181, 1586
Hin6I GCGC 2 cut(s) 179, 1584
HinP1I GCGC 2 cut(s) 179, 1584
HincII GTYRAC 2 cut(s) 1188, 2071
HindII GTYRAC 2 cut(s) 1188, 2071
HindIII AAGCTT 1 cut(s) 1900
HinfI GANTC 9 cut(s) 16, 458, 552, 684, 1352, 1555, 1706, 2468, 2615
HpaII CCGG 4 cut(s) 1253, 1346, 1488, 2861
HphI GGTGA 5 cut(s) 1163, 1477, 1963, 2111, 2745
Hpy166II GTNNAC 8 cut(s) 112, 130, 532, 1188, 1618, 2071, 2304, 2780
Hpy188I TCNGA 9 cut(s) 21, 187, 288, 426, 884, 1139, 2060, 2377, 2713
Hpy8I GTNNAC 8 cut(s) 112, 130, 532, 1188, 1618, 2071, 2304, 2780
HpyAV CCTTC 7 cut(s) 417, 542, 1257, 1417, 1890, 2085, 2587
HpyCH4III ACNGT 8 cut(s) 1185, 1295, 1527, 1637, 2047, 2462, 2717, 2841
HpyCH4IV ACGT 4 cut(s) 114, 528, 2106, 2543
HpyF3I CTNAG 4 cut(s) 184, 881, 2628, 2678
HpySE526I ACGT 4 cut(s) 114, 528, 2106, 2543
HspAI GCGC 2 cut(s) 179, 1584
Kpn2I TCCGGA 1 cut(s) 2860
Ksp22I TGATCA 1 cut(s) 637
LmnI GCTCC 1 cut(s) 434
Lsp1109I GCAGC 7 cut(s) 95, 278, 287, 992, 1783, 2230, 2712
MaeI CTAG 4 cut(s) 317, 681, 2030, 2487
MaeII ACGT 4 cut(s) 114, 528, 2106, 2543
MaeIII GTNAC 6 cut(s) 239, 1450, 1483, 1527, 2456, 2569
MfeI CAATTG 2 cut(s) 1243, 2247
MflI RGATCY 6 cut(s) 283, 815, 1069, 1380, 2186, 2658
MhlI GDGCHC 3 cut(s) 381, 431, 2731
MlsI TGGCCA 1 cut(s) 2770
MluNI TGGCCA 1 cut(s) 2770
MlyI GAGTC 5 cut(s) 452, 693, 1361, 1715, 2462
MmeI TCCRAC 5 cut(s) 940, 1624, 2074, 2083, 2897
Mox20I TGGCCA 1 cut(s) 2770
Mph1103I ATGCAT 2 cut(s) 1567, 1759
MroI TCCGGA 1 cut(s) 2860
MroXI GAANNNNTTC 2 cut(s) 1048, 2868
MscI TGGCCA 1 cut(s) 2770
MslI CAYNNNNRTG 4 cut(s) 173, 252, 2595, 2802
Msp20I TGGCCA 1 cut(s) 2770
MspA1I CMGCKG 3 cut(s) 86, 269, 2066
MspI CCGG 4 cut(s) 1253, 1346, 1488, 2861
MspR9I CCNGG 4 cut(s) 220, 1347, 1489, 1961
MunI CAATTG 2 cut(s) 1243, 2247
Mva1269I GAATGC 1 cut(s) 1024
MvaI CCWGG 2 cut(s) 220, 1961
NciI CCSGG 2 cut(s) 1347, 1489
NcoI CCATGG 2 cut(s) 78, 996
NdeI CATATG 2 cut(s) 699, 1534
NlaIV GGNNCC 5 cut(s) 1333, 1579, 1787, 2519, 2623
NmuCI GTSAC 1 cut(s) 1483
NsiI ATGCAT 2 cut(s) 1567, 1759
NspI RCATGY 2 cut(s) 2600, 2694
NspV TTCGAA 1 cut(s) 2089
PaeR7I CTCGAG 1 cut(s) 581
PagI TCATGA 2 cut(s) 2254, 2737
PciI ACATGT 1 cut(s) 2690
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PctI GAATGC 1 cut(s) 1024
PdmI GAANNNNTTC 2 cut(s) 1048, 2868
PfeI GAWTC 4 cut(s) 16, 552, 1555, 2615
PflMI CCANNNNNTGG 3 cut(s) 1341, 1374, 1391
PkrI GCNGC 8 cut(s) 76, 85, 268, 277, 982, 1773, 2245, 2702
PleI GAGTC 5 cut(s) 452, 692, 1360, 1714, 2462
PpsI GAGTC 5 cut(s) 452, 692, 1360, 1714, 2462
Ppu21I YACGTR 1 cut(s) 529
PscI ACATGT 1 cut(s) 2690
PshAI GACNNNNGTC 2 cut(s) 221, 683
Psp124BI GAGCTC 1 cut(s) 431
Psp6I CCWGG 2 cut(s) 218, 1959
PspGI CCWGG 2 cut(s) 218, 1959
PspN4I GGNNCC 5 cut(s) 1333, 1579, 1787, 2519, 2623
PspPI GGNCC 5 cut(s) 320, 1331, 1874, 2315, 2518
PspXI VCTCGAGB 1 cut(s) 581
PsuI RGATCY 6 cut(s) 283, 815, 1069, 1380, 2186, 2658
PvuII CAGCTG 3 cut(s) 86, 269, 2066
RsaI GTAC 4 cut(s) 542, 1396, 2585, 2609
RsaNI GTAC 4 cut(s) 541, 1395, 2584, 2608
RseI CAYNNNNRTG 4 cut(s) 173, 252, 2595, 2802
SacI GAGCTC 1 cut(s) 431
SatI GCNGC 8 cut(s) 75, 84, 267, 276, 981, 1772, 2244, 2701
Sau96I GGNCC 5 cut(s) 320, 1331, 1874, 2315, 2518
SchI GAGTC 5 cut(s) 452, 693, 1361, 1715, 2462
ScrFI CCNGG 4 cut(s) 220, 1347, 1489, 1961
SduI GDGCHC 3 cut(s) 381, 431, 2731
SfcI CTRYAG 2 cut(s) 1065, 1515
Sfr274I CTCGAG 1 cut(s) 581
SfuI TTCGAA 1 cut(s) 2089
SinI GGWCC 4 cut(s) 320, 1331, 1874, 2315
SlaI CTCGAG 1 cut(s) 581
SmiMI CAYNNNNRTG 4 cut(s) 173, 252, 2595, 2802
SmlI CTYRAG 4 cut(s) 581, 2174, 2504, 2730
SmoI CTYRAG 4 cut(s) 581, 2174, 2504, 2730
SnaBI TACGTA 1 cut(s) 529
SsiI CCGC 1 cut(s) 75
SspI AATATT 3 cut(s) 2291, 2322, 2550
SspMI CTAG 4 cut(s) 317, 681, 2030, 2487
SstI GAGCTC 1 cut(s) 431
StyD4I CCNGG 4 cut(s) 218, 1345, 1487, 1959
StyI CCWWGG 5 cut(s) 78, 316, 732, 996, 1621
TaaI ACNGT 8 cut(s) 1185, 1295, 1527, 1637, 2047, 2462, 2717, 2841
TaiI ACGT 4 cut(s) 117, 531, 2109, 2546
TaqI TCGA 5 cut(s) 582, 607, 830, 2089, 2929
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 4 cut(s) 16, 552, 1555, 2615
TscAI CASTG 4 cut(s) 1425, 1483, 2026, 2722
TseFI GTSAC 1 cut(s) 1483
TseI GCWGC 7 cut(s) 83, 266, 275, 980, 1771, 2243, 2700
Tsp45I GTSAC 1 cut(s) 1483
TspGWI ACGGA 4 cut(s) 1351, 1718, 1859, 2420
TspRI CASTG 4 cut(s) 1425, 1483, 2026, 2722
Van91I CCANNNNNTGG 3 cut(s) 1341, 1374, 1391
VpaK11BI GGWCC 4 cut(s) 320, 1331, 1874, 2315
XagI CCTNNNNNAGG 1 cut(s) 2616
XapI RAATTY 7 cut(s) 826, 887, 1282, 1627, 2359, 2398, 2479
XceI RCATGY 2 cut(s) 2600, 2694
XcmI CCANNNNNNNNNTGG 1 cut(s) 2763
XhoI CTCGAG 1 cut(s) 581
XmaJI CCTAGG 1 cut(s) 316
XmiI GTMKAC 4 cut(s) 129, 531, 1617, 2303
XmnI GAANNNNTTC 2 cut(s) 1048, 2868
XspI CTAG 4 cut(s) 317, 681, 2030, 2487
Zsp2I ATGCAT 2 cut(s) 1567, 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.