Rw5G033300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011546
Physical Location & Seq
Reverse (-)
56471870 .. 56472830
961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033300.1

Sequence Viewer

Length: 624 bp
ATGCGGTGCGATCAGACAATGCGCCGGCTCCTGCTCCTGCTCCCAAACTCGCAAACCCGCCTCCCCCGCCTCCCCCCTCCTCCCGCGGCTTCTCCGGCACTTACTCCGACCGCTTCTCCTCCGGCATCCCCTCCGACTCACTCTCCTCCGCCTGCGGCTTCTCCAAAATCCTCTCCACCAACTTCGACGCCTCCGGCCGCCGCGCCGACCGTCTCGCCGGCAACTCCTCCTCCGGCGTCACCCGTTTCTCCAGCGGCTAGCCCCTCCGCAAATGGGTCGTCGCCGGTTCAAGCCCCGGCCACGCAGCCGCCGACTGTGGCCTCTCCCGTTGCGACTCCGGTGAGCGCTCCGAAGTCAAGCCCATCTGGGGCTCCGCCGGTTGCAGAGACTCCCGAGACTGCGGCAACCCCATCCTCGTCGAGCATTCCTTCGTCTGCAACCCCGTCGGAGTCGCCGGCGATGTTCCCGTCGAGCAGTAGCCCACCAAGTCCGACGCCGGCGGGTCTGTCGCCTGAGGGCGCGGCGGCGAGTCCCGCTAGTGATGATACGTCGGGTTCGGAGCCCAAGTATGGAGTTGGGTTAATTTTGAGTGGGCTCTCTATCTGGGTCGCTTTGGCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

20.01

Weight (kDa)

6.54

Isoelectric Point (pI)

107.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 86, 203, 521
AcoI YGGCCR 2 cut(s) 195, 297
AcyI GRCGYC 3 cut(s) 188, 236, 494
AfeI AGCGCT 1 cut(s) 346
AfiI CCNNNNNNNGG 3 cut(s) 273, 367, 569
AgsI TTSAA 1 cut(s) 290
Alw26I GTCTC 3 cut(s) 217, 380, 389
Ama87I CYCGRG 1 cut(s) 392
Aor51HI AGCGCT 1 cut(s) 346
AoxI GGCC 3 cut(s) 195, 297, 318
ApeKI GCWGC 1 cut(s) 304
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspLEI GCGC 4 cut(s) 24, 205, 347, 521
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 231, 352
AsuNHI GCTAGC 1 cut(s) 257
AvaI CYCGRG 1 cut(s) 392
AxyI CCTNAGG 1 cut(s) 513
BanII GRGCYC 3 cut(s) 373, 564, 597
BbvI GCAGC 1 cut(s) 316
BccI CCATC 2 cut(s) 370, 418
BcgI CGANNNNNNTGC 4 cut(s) 258, 292, 426, 460
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 3 cut(s) 217, 380, 389
BfaI CTAG 2 cut(s) 258, 537
BfoI RGCGCY 1 cut(s) 348
Bme1390I CCNGG 1 cut(s) 296
BmeT110I CYCGRG 1 cut(s) 392
BmiI GGNNCC 3 cut(s) 29, 372, 561
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 134
BmtI GCTAGC 1 cut(s) 261
BpmI CTGGAG 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 296
BsaHI GRCGYC 3 cut(s) 188, 236, 494
BsaJI CCNNGG 2 cut(s) 84, 294
BsaWI WCCGGW 1 cut(s) 337
BsaXI ACNNNNNCTCC 8 cut(s) 100, 127, 130, 157, 214, 244, 551, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 273, 367, 569
Bse118I RCCGGY 6 cut(s) 24, 217, 283, 376, 454, 496
Bse21I CCTNAGG 1 cut(s) 513
BseDI CCNNGG 2 cut(s) 84, 294
BseGI GGATG 2 cut(s) 125, 410
BseLI CCNNNNNNNGG 3 cut(s) 273, 367, 569
BseMII CTCAG 1 cut(s) 504
BseRI GAGGAG 5 cut(s) 69, 108, 135, 216, 219
BseX3I CGGCCG 1 cut(s) 195
BseXI GCAGC 1 cut(s) 316
Bsh1236I CGCG 3 cut(s) 86, 203, 521
Bsh1285I CGRYCG 3 cut(s) 111, 198, 210
BshFI GGCC 3 cut(s) 197, 299, 320
BsiEI CGRYCG 3 cut(s) 111, 198, 210
BsiHKCI CYCGRG 1 cut(s) 392
BslFI GGGAC 1 cut(s) 516
BslI CCNNNNNNNGG 3 cut(s) 273, 367, 569
BsmAI GTCTC 3 cut(s) 217, 380, 389
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 516
BsmI GAATGC 1 cut(s) 423
BsnI GGCC 3 cut(s) 197, 299, 320
BsoBI CYCGRG 1 cut(s) 392
Bsp1286I GDGCHC 3 cut(s) 373, 564, 597
Bsp143I GATC 1 cut(s) 10
BspANI GGCC 3 cut(s) 197, 299, 320
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 3 cut(s) 86, 203, 521
BspLI GGNNCC 3 cut(s) 29, 372, 561
BspOI GCTAGC 1 cut(s) 261
BsrFI RCCGGY 6 cut(s) 24, 217, 283, 376, 454, 496
BssAI RCCGGY 6 cut(s) 24, 217, 283, 376, 454, 496
BssECI CCNNGG 2 cut(s) 84, 294
BssMI GATC 1 cut(s) 10
BssNI GRCGYC 3 cut(s) 188, 236, 494
Bst4CI ACNGT 2 cut(s) 211, 316
BstACI GRCGYC 3 cut(s) 188, 236, 494
BstC8I GCNNGC 6 cut(s) 26, 153, 219, 259, 456, 498
BstDEI CTNAG 1 cut(s) 513
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 2 cut(s) 125, 410
BstFNI CGCG 3 cut(s) 86, 203, 521
BstH2I RGCGCY 1 cut(s) 348
BstHHI GCGC 4 cut(s) 24, 205, 347, 521
BstKTI GATC 1 cut(s) 13
BstMAI GTCTC 3 cut(s) 217, 380, 389
BstMBI GATC 1 cut(s) 10
BstMCI CGRYCG 3 cut(s) 111, 198, 210
BstMWI GCNNNNNNNGC 3 cut(s) 66, 95, 533
BstSCI CCNGG 1 cut(s) 294
BstUI CGCG 3 cut(s) 86, 203, 521
BstV1I GCAGC 1 cut(s) 316
BstZI CGGCCG 1 cut(s) 195
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 3 cut(s) 197, 299, 320
BtgI CCRYGG 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 473
BtsCI GGATG 2 cut(s) 125, 410
Cac8I GCNNGC 6 cut(s) 26, 153, 219, 259, 456, 498
CfoI GCGC 4 cut(s) 24, 205, 347, 521
Cfr10I RCCGGY 6 cut(s) 24, 217, 283, 376, 454, 496
Cfr42I CCGCGG 1 cut(s) 87
CseI GACGC 3 cut(s) 196, 225, 502
DdeI CTNAG 1 cut(s) 513
DpnI GATC 1 cut(s) 12
DpnII GATC 1 cut(s) 10
EaeI YGGCCR 2 cut(s) 195, 297
EagI CGGCCG 1 cut(s) 195
EciI GGCGGA 2 cut(s) 138, 363
EclXI CGGCCG 1 cut(s) 195
Eco24I GRGCYC 3 cut(s) 373, 564, 597
Eco47III AGCGCT 1 cut(s) 346
Eco52I CGGCCG 1 cut(s) 195
Eco81I CCTNAGG 1 cut(s) 513
Eco88I CYCGRG 1 cut(s) 392
EcoT38I GRGCYC 3 cut(s) 373, 564, 597
Esp3I CGTCTC 1 cut(s) 217
FaiI YATR 1 cut(s) 570
FaqI GGGAC 1 cut(s) 516
FauI CCCGC 5 cut(s) 65, 74, 91, 493, 541
FokI GGATG 2 cut(s) 112, 397
FriOI GRGCYC 3 cut(s) 373, 564, 597
FspBI CTAG 2 cut(s) 258, 537
GlaI GCGC 4 cut(s) 23, 204, 346, 520
GsuI CTGGAG 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 348
HaeIII GGCC 3 cut(s) 197, 299, 320
HgaI GACGC 3 cut(s) 196, 225, 502
HhaI GCGC 4 cut(s) 24, 205, 347, 521
Hin1I GRCGYC 3 cut(s) 188, 236, 494
Hin6I GCGC 4 cut(s) 22, 203, 345, 519
HinP1I GCGC 4 cut(s) 22, 203, 345, 519
HinfI GANTC 5 cut(s) 136, 334, 388, 449, 529
HphI GGTGA 2 cut(s) 231, 352
Hpy188I TCNGA 7 cut(s) 15, 108, 135, 351, 448, 492, 559
Hpy188III TCNNGA 1 cut(s) 392
Hpy99I CGWCG 7 cut(s) 190, 283, 421, 448, 472, 496, 553
HpyAV CCTTC 1 cut(s) 438
HpyCH4III ACNGT 2 cut(s) 211, 316
HpyCH4IV ACGT 1 cut(s) 548
HpyCH4V TGCA 2 cut(s) 383, 437
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 95, 533
HpyF3I CTNAG 1 cut(s) 513
HpySE526I ACGT 1 cut(s) 548
Hsp92I GRCGYC 3 cut(s) 188, 236, 494
HspAI GCGC 4 cut(s) 22, 203, 345, 519
KroI GCCGGC 4 cut(s) 24, 217, 454, 496
KroNI GCCGGC 4 cut(s) 26, 219, 456, 498
KspI CCGCGG 1 cut(s) 87
Kzo9I GATC 1 cut(s) 10
LmnI GCTCC 6 cut(s) 33, 39, 45, 352, 376, 559
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 1 cut(s) 134
MaeI CTAG 2 cut(s) 258, 537
MaeII ACGT 1 cut(s) 548
MaeIII GTNAC 1 cut(s) 237
MalI GATC 1 cut(s) 12
MboI GATC 1 cut(s) 10
MhlI GDGCHC 3 cut(s) 373, 564, 597
MluCI AATT 1 cut(s) 582
MlyI GAGTC 5 cut(s) 130, 328, 382, 458, 538
MmeI TCCRAC 4 cut(s) 131, 158, 426, 515
MreI CGCCGGCG 2 cut(s) 454, 496
MroNI GCCGGC 4 cut(s) 24, 217, 454, 496
MseI TTAA 1 cut(s) 581
MspA1I CMGCKG 2 cut(s) 86, 254
MspR9I CCNGG 1 cut(s) 296
Mva1269I GAATGC 1 cut(s) 423
MvnI CGCG 3 cut(s) 86, 203, 521
MwoI GCNNNNNNNGC 3 cut(s) 66, 95, 533
NaeI GCCGGC 4 cut(s) 26, 219, 456, 498
NciI CCSGG 1 cut(s) 296
NdeII GATC 1 cut(s) 10
NgoMIV GCCGGC 4 cut(s) 24, 217, 454, 496
NheI GCTAGC 1 cut(s) 257
NlaIV GGNNCC 3 cut(s) 29, 372, 561
NmuCI GTSAC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PctI GAATGC 1 cut(s) 423
PdiI GCCGGC 4 cut(s) 26, 219, 456, 498
PleI GAGTC 5 cut(s) 130, 328, 382, 457, 537
PpsI GAGTC 5 cut(s) 130, 328, 382, 457, 537
PspN4I GGNNCC 3 cut(s) 29, 372, 561
SacII CCGCGG 1 cut(s) 87
SaqAI TTAA 1 cut(s) 581
Sau3AI GATC 1 cut(s) 10
SchI GAGTC 5 cut(s) 130, 328, 382, 458, 538
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 3 cut(s) 373, 564, 597
SetI ASST 1 cut(s) 551
SfaNI GCATC 1 cut(s) 134
Sfr303I CCGCGG 1 cut(s) 87
SgrAI CRCCGGYG 2 cut(s) 454, 496
SgrBI CCGCGG 1 cut(s) 87
Sse9I AATT 1 cut(s) 582
SspMI CTAG 2 cut(s) 258, 537
StyD4I CCNGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 211, 316
TaiI ACGT 1 cut(s) 551
TaqI TCGA 3 cut(s) 185, 419, 470
TasI AATT 1 cut(s) 582
TauI GCSGC 9 cut(s) 89, 158, 200, 203, 257, 310, 404, 524, 527
Tru1I TTAA 1 cut(s) 581
Tru9I TTAA 1 cut(s) 581
TseFI GTSAC 1 cut(s) 237
TseI GCWGC 1 cut(s) 304
Tsp45I GTSAC 1 cut(s) 237
XspI CTAG 2 cut(s) 258, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.