Rorug05G0311500

P-loop NTPase domain-containing protein LPA1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004419
Physical Location & Seq
Reverse (-)
36671192 .. 36673112
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0311500.1

Sequence Viewer

Length: 1509 bp
ATGAGTCCTTCAACAATGGCCATCCTTATAGTCTTCCTCGTGTTCATCTGGTTTCTCATCACTACAGTCTCAAAACCAAAGCACAAATCAAAGCTACCTCCTGGCCCTCGGTCACTACCAATCATTGGTAACCTTCATATGCTAGGCAGCCTCCCACACCGTAGCCTCCAACATTTGGCCGAAAGATATGGACCAATAATGTCTATTCATCTTGGCAATATACCCGCCATAATAGTCTCATCTCCAAAAGCTGCAGAGCTGTTCCTCAAAACCCATGACCATAATTTTGCCAATCGGCCCAAAACCCAGGTCTCTCAGTGCATAACTTACGGTGCAAAGGGCATGGCCTTTTCCGAATACGGCCCATTTTGGCGTCATGCGAAGAAGATTTGCACAACCGAACTTACGTGTCCCTCGAAAATTCAAGCTTTTGCACCGCGGCGAAGGGAGGAGATAGGGCTATTGGTGCAGTCACTGAAGAGAGCTGGAGAGGAAAGTCAATTGGTGGATATTAGTGACAAAATTGGTGAGGTTGTTGAGGATATAATGTATAGGATGGTACTGGGTGGCAAAAAGAATGATTGGTTGGGTTTGAAGGAGATTATTGAGGAGGCTGTATCGTTGGTCGGAGCATTCAACATAGCTGATTATGTGCCATTCCTCGGTCCATTTGATTTTCAGGGTTTGACCAAGAGCTTAAAGAAAGTTAGCAAGAGAATCGACCAACTCTTGGAGAAGATAATTGAGGACCATGAAGTTAACACTGAAAGGGGGAAACATGGCCAGCATGAGGACTTTGTAGACGTGTTGCTTTCAAACCAACGGTTAAACCCGAATGACAAGAGTTTAATTCCTCTTGATCGAACAAACGTGAAAGGCATCTTATTAGACATGTTGGTTGCATCTTTTGATACTTCAACCGTTGCAATTGTTTGGACCTTTGCCGAGCTCTTGAGGCATCCAAGGGTCATGAAGAATCTCCAAGAAGAGCTCCAAACTGTAGTTGGTATGGACCGAGTGGTGGATGAGACTGATTTGCCACATCTGGTTTACTTGAATATGGTGGTTAAGGAGAGCTTCAGACTGCACCCAGTTGCACCACTTCTAGTCCCACATGAATCCATCGAGGATATTACAATTGATGGATACTACATACCCAAGAAATCGCGGATCATGGTAAACGTCTGGAGCATTGGAAGGGATCCTAATGTTTGGTCAGAAAAGGTTGAAGAATTTTATCCTGAAAGGTTCATGAACAACAATATGGACCTCCGGGGACATGATTTTCAGCTCCTCCCATTTGGAGCTGGCCGAAGAGGGTGTCCGGGTATGCAATTAGGGCTAACCACAGTTCGGCTAGTTCTTGCACAACTGGTGCACTGCTTTAACTGGGATCTCCCAAATTGCTTGCTACCTCAAGACTTGGACATGACAGAGAAGTTCGGCTTATCAATGCCAAAAGCCGAACACTTGCTTGCCAAGCCAACATACCGTCTTACTCTTCTGTAG

Protein Analysis

502

Amino Acids

56.93

Weight (kDa)

7.71

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 487 2.7e-103 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 125, 175, 730
AccI GTMKAC 1 cut(s) 801
AccII CGCG 2 cut(s) 439, 1168
AciI CCGC 4 cut(s) 225, 437, 439, 1168
AclWI GGATC 4 cut(s) 1178, 1196, 1209, 1401
AcoI YGGCCR 4 cut(s) 18, 177, 781, 1309
AcsI RAATTY 2 cut(s) 420, 1232
AcuI CTGAAG 2 cut(s) 497, 1063
AcyI GRCGYC 1 cut(s) 373
AfaI GTAC 1 cut(s) 561
AfiI CCNNNNNNNGG 7 cut(s) 125, 175, 662, 730, 790, 1021, 1353
AflIII ACRYGT 3 cut(s) 407, 804, 891
AgsI TTSAA 8 cut(s) 12, 425, 595, 637, 816, 918, 1057, 1229
AjiI CACGTC 1 cut(s) 805
AjnI CCWGG 2 cut(s) 100, 306
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
Alw21I GWGCWC 3 cut(s) 951, 993, 1380
Alw26I GTCTC 4 cut(s) 73, 241, 316, 1022
Alw44I GTGCAC 1 cut(s) 1376
AlwI GGATC 4 cut(s) 1178, 1196, 1209, 1401
AlwNI CAGNNNCTG 1 cut(s) 475
AoxI GGCC 8 cut(s) 18, 103, 177, 296, 345, 361, 781, 1309
ApaLI GTGCAC 1 cut(s) 1376
ApeKI GCWGC 2 cut(s) 147, 251
ApoI RAATTY 2 cut(s) 420, 1232
ArsI GACNNNNNNTTYG 6 cut(s) 269, 294, 301, 326, 1306, 1338
AspS9I GGNCC 9 cut(s) 104, 191, 297, 362, 665, 748, 936, 1012, 1267
AsuC2I CCSGG 2 cut(s) 1274, 1326
AsuHPI GGTGA 1 cut(s) 539
AvaII GGWCC 6 cut(s) 191, 665, 748, 936, 1012, 1267
BaeGI GKGCMC 1 cut(s) 1380
BalI TGGCCA 2 cut(s) 20, 783
BamHI GGATCC 1 cut(s) 1201
BanII GRGCYC 2 cut(s) 951, 993
BauI CACGAG 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 3 cut(s) 951, 993, 1380
BbvI GCAGC 2 cut(s) 159, 238
BccI CCATC 4 cut(s) 29, 550, 1130, 1136
BceAI ACGGC 1 cut(s) 376
BcgI CGANNNNNNTGC 2 cut(s) 700, 734
BciT130I CCWGG 2 cut(s) 102, 308
BciVI GTATCC 1 cut(s) 1139
BcnI CCSGG 2 cut(s) 1274, 1326
BcoDI GTCTC 4 cut(s) 73, 241, 316, 1022
BfaI CTAG 3 cut(s) 143, 1106, 1358
BfmI CTRYAG 4 cut(s) 63, 252, 999, 1505
BfuI GTATCC 1 cut(s) 1139
BisI GCNGC 3 cut(s) 148, 252, 440
BlsI GCNGC 3 cut(s) 149, 253, 441
Bme1390I CCNGG 4 cut(s) 102, 308, 1274, 1326
Bme18I GGWCC 6 cut(s) 191, 665, 748, 936, 1012, 1267
BmgBI CACGTC 1 cut(s) 805
BmgT120I GGNCC 9 cut(s) 104, 191, 297, 362, 665, 748, 936, 1012, 1267
BmiI GGNNCC 1 cut(s) 1203
BmrFI CCNGG 4 cut(s) 102, 308, 1274, 1326
BmrI ACTGGG 3 cut(s) 572, 1085, 1399
BmsI GCATC 3 cut(s) 888, 911, 967
BmuI ACTGGG 3 cut(s) 572, 1085, 1399
BpiI GAAGAC 1 cut(s) 25
BpmI CTGGAG 2 cut(s) 507, 1207
BpuEI CTTGAG 2 cut(s) 973, 1401
BpuMI CCSGG 2 cut(s) 1274, 1326
BsaAI YACGTR 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 373
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 6 cut(s) 107, 306, 437, 661, 962, 1273
Bsc4I CCNNNNNNNGG 7 cut(s) 125, 175, 662, 730, 790, 1021, 1353
Bse1I ACTGG 4 cut(s) 567, 1091, 1377, 1394
BseBI CCWGG 2 cut(s) 102, 308
BseDI CCNNGG 6 cut(s) 107, 306, 437, 661, 962, 1273
BseGI GGATG 4 cut(s) 21, 561, 958, 1030
BseLI CCNNNNNNNGG 7 cut(s) 125, 175, 662, 730, 790, 1021, 1353
BseMII CTCAG 1 cut(s) 329
BseNI ACTGG 4 cut(s) 567, 1091, 1377, 1394
BseRI GAGGAG 3 cut(s) 464, 623, 1283
BseSI GKGCMC 1 cut(s) 1380
BseXI GCAGC 2 cut(s) 159, 238
BsgI GTGCAG 2 cut(s) 488, 1070
Bsh1236I CGCG 2 cut(s) 439, 1168
BshFI GGCC 8 cut(s) 20, 105, 179, 298, 347, 363, 783, 1311
BsiHKAI GWGCWC 3 cut(s) 951, 993, 1380
BsiSI CCGG 2 cut(s) 1273, 1325
BslFI GGGAC 3 cut(s) 396, 1094, 1290
BslI CCNNNNNNNGG 7 cut(s) 125, 175, 662, 730, 790, 1021, 1353
BsmAI GTCTC 4 cut(s) 73, 241, 316, 1022
BsmFI GGGAC 3 cut(s) 396, 1094, 1290
BsmI GAATGC 1 cut(s) 632
BsnI GGCC 8 cut(s) 20, 105, 179, 298, 347, 363, 783, 1311
Bso31I GGTCTC 1 cut(s) 316
Bsp1286I GDGCHC 3 cut(s) 951, 993, 1380
Bsp143I GATC 4 cut(s) 859, 1170, 1201, 1393
BspACI CCGC 4 cut(s) 225, 437, 439, 1168
BspANI GGCC 8 cut(s) 20, 105, 179, 298, 347, 363, 783, 1311
BspCNI CTCAG 1 cut(s) 328
BspFNI CGCG 2 cut(s) 439, 1168
BspHI TCATGA 2 cut(s) 969, 1251
BspLI GGNNCC 1 cut(s) 1203
BspMAI CTGCAG 1 cut(s) 256
BspPI GGATC 4 cut(s) 1178, 1196, 1209, 1401
BspQI GCTCTTC 1 cut(s) 981
BspTNI GGTCTC 1 cut(s) 316
BsrI ACTGG 4 cut(s) 567, 1091, 1377, 1394
BssECI CCNNGG 6 cut(s) 107, 306, 437, 661, 962, 1273
BssMI GATC 4 cut(s) 859, 1170, 1201, 1393
BssNI GRCGYC 1 cut(s) 373
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 962
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 2 cut(s) 102, 308
Bst4CI ACNGT 8 cut(s) 67, 161, 332, 825, 922, 1000, 1351, 1493
Bst6I CTCTTC 4 cut(s) 473, 981, 1309, 1506
BstACI GRCGYC 1 cut(s) 373
BstBAI YACGTR 1 cut(s) 408
BstC8I GCNNGC 4 cut(s) 785, 1309, 1409, 1476
BstDEI CTNAG 1 cut(s) 315
BstDSI CCRYGG 1 cut(s) 437
BstEII GGTNACC 1 cut(s) 128
BstF5I GGATG 4 cut(s) 21, 561, 958, 1030
BstFNI CGCG 2 cut(s) 439, 1168
BstKTI GATC 4 cut(s) 862, 1173, 1204, 1396
BstMAI GTCTC 4 cut(s) 73, 241, 316, 1022
BstMBI GATC 4 cut(s) 859, 1170, 1201, 1393
BstMWI GCNNNNNNNGC 4 cut(s) 466, 955, 1339, 1480
BstNI CCWGG 2 cut(s) 102, 308
BstNSI RCATGY 1 cut(s) 895
BstPI GGTNACC 1 cut(s) 128
BstSCI CCNGG 4 cut(s) 100, 306, 1272, 1324
BstSFI CTRYAG 4 cut(s) 63, 252, 999, 1505
BstSLI GKGCMC 1 cut(s) 1380
BstUI CGCG 2 cut(s) 439, 1168
BstV1I GCAGC 2 cut(s) 159, 238
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 2 cut(s) 1201, 1393
BstYI RGATCY 2 cut(s) 1201, 1393
BsuI GTATCC 1 cut(s) 1139
BsuRI GGCC 8 cut(s) 20, 105, 179, 298, 347, 363, 783, 1311
BtgI CCRYGG 1 cut(s) 437
BtrI CACGTC 1 cut(s) 805
BtsCI GGATG 4 cut(s) 21, 561, 958, 1030
BtsI GCAGTG 1 cut(s) 1378
BtsIMutI CAGTG 4 cut(s) 323, 473, 762, 1378
Cac8I GCNNGC 4 cut(s) 785, 1309, 1409, 1476
CaiI CAGNNNCTG 1 cut(s) 475
CciI TCATGA 2 cut(s) 969, 1251
Cfr13I GGNCC 9 cut(s) 104, 191, 297, 362, 665, 748, 936, 1012, 1267
Cfr42I CCGCGG 1 cut(s) 440
CseI GACGC 1 cut(s) 362
Csp6I GTAC 1 cut(s) 560
CviQI GTAC 1 cut(s) 560
DdeI CTNAG 1 cut(s) 315
DpnI GATC 4 cut(s) 861, 1172, 1203, 1395
DpnII GATC 4 cut(s) 859, 1170, 1201, 1393
EaeI YGGCCR 4 cut(s) 18, 177, 781, 1309
Eam1104I CTCTTC 4 cut(s) 473, 981, 1309, 1506
EarI CTCTTC 4 cut(s) 473, 981, 1309, 1506
Ecl136II GAGCTC 2 cut(s) 949, 991
Eco130I CCWWGG 1 cut(s) 962
Eco24I GRGCYC 2 cut(s) 951, 993
Eco31I GGTCTC 1 cut(s) 316
Eco47I GGWCC 6 cut(s) 191, 665, 748, 936, 1012, 1267
Eco53kI GAGCTC 2 cut(s) 949, 991
Eco57I CTGAAG 2 cut(s) 497, 1063
Eco91I GGTNACC 1 cut(s) 128
EcoICRI GAGCTC 2 cut(s) 949, 991
EcoO65I GGTNACC 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 100, 306
EcoT14I CCWWGG 1 cut(s) 962
EcoT38I GRGCYC 2 cut(s) 951, 993
ErhI CCWWGG 1 cut(s) 962
FalI AAGNNNNNCTT 4 cut(s) 1061, 1093, 1430, 1462
FaqI GGGAC 3 cut(s) 396, 1094, 1290
FauI CCCGC 1 cut(s) 232
FauNDI CATATG 1 cut(s) 138
FblI GTMKAC 1 cut(s) 801
Fnu4HI GCNGC 3 cut(s) 148, 252, 440
FokI GGATG 4 cut(s) 8, 568, 945, 1037
FriOI GRGCYC 2 cut(s) 951, 993
Fsp4HI GCNGC 3 cut(s) 148, 252, 440
FspBI CTAG 3 cut(s) 143, 1106, 1358
GluI GCNGC 3 cut(s) 148, 252, 440
GsuI CTGGAG 2 cut(s) 507, 1207
HaeIII GGCC 8 cut(s) 20, 105, 179, 298, 347, 363, 783, 1311
HapII CCGG 2 cut(s) 1273, 1325
HgaI GACGC 1 cut(s) 362
Hin1I GRCGYC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 4 cut(s) 4, 717, 976, 1118
HpaI GTTAAC 1 cut(s) 760
HpaII CCGG 2 cut(s) 1273, 1325
HphI GGTGA 1 cut(s) 539
Hpy166II GTNNAC 5 cut(s) 760, 802, 1051, 1180, 1378
Hpy188I TCNGA 4 cut(s) 355, 629, 1082, 1219
Hpy188III TCNNGA 7 cut(s) 857, 952, 970, 1186, 1241, 1252, 1418
Hpy8I GTNNAC 5 cut(s) 760, 802, 1051, 1180, 1378
HpyAV CCTTC 5 cut(s) 18, 143, 438, 589, 1191
HpyCH4III ACNGT 8 cut(s) 67, 161, 332, 825, 922, 1000, 1351, 1493
HpyCH4IV ACGT 4 cut(s) 407, 804, 870, 1182
HpyF10VI GCNNNNNNNGC 4 cut(s) 466, 955, 1339, 1480
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 4 cut(s) 407, 804, 870, 1182
Hsp92I GRCGYC 1 cut(s) 373
KspAI GTTAAC 1 cut(s) 760
KspI CCGCGG 1 cut(s) 440
Kzo9I GATC 4 cut(s) 859, 1170, 1201, 1393
LguI GCTCTTC 1 cut(s) 981
LmnI GCTCC 5 cut(s) 629, 996, 1188, 1296, 1304
Lsp1109I GCAGC 2 cut(s) 159, 238
LweI GCATC 3 cut(s) 888, 911, 967
MaeI CTAG 3 cut(s) 143, 1106, 1358
MaeII ACGT 4 cut(s) 407, 804, 870, 1182
MaeIII GTNAC 4 cut(s) 111, 128, 471, 515
MalI GATC 4 cut(s) 861, 1172, 1203, 1395
MboI GATC 4 cut(s) 859, 1170, 1201, 1393
MfeI CAATTG 3 cut(s) 500, 927, 1137
MflI RGATCY 2 cut(s) 1201, 1393
MhlI GDGCHC 3 cut(s) 951, 993, 1380
MlsI TGGCCA 2 cut(s) 20, 783
MluNI TGGCCA 2 cut(s) 20, 783
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 2 cut(s) 193, 607
Mox20I TGGCCA 2 cut(s) 20, 783
MscI TGGCCA 2 cut(s) 20, 783
MseI TTAA 6 cut(s) 698, 759, 827, 848, 1068, 1386
Msp20I TGGCCA 2 cut(s) 20, 783
MspA1I CMGCKG 1 cut(s) 439
MspI CCGG 2 cut(s) 1273, 1325
MspR9I CCNGG 4 cut(s) 102, 308, 1274, 1326
MunI CAATTG 3 cut(s) 500, 927, 1137
Mva1269I GAATGC 1 cut(s) 632
MvaI CCWGG 2 cut(s) 102, 308
MvnI CGCG 2 cut(s) 439, 1168
MwoI GCNNNNNNNGC 4 cut(s) 466, 955, 1339, 1480
NciI CCSGG 2 cut(s) 1274, 1326
NdeI CATATG 1 cut(s) 138
NdeII GATC 4 cut(s) 859, 1170, 1201, 1393
NlaIV GGNNCC 1 cut(s) 1203
NmeAIII GCCGAG 1 cut(s) 970
NmuCI GTSAC 3 cut(s) 111, 471, 515
NspI RCATGY 1 cut(s) 895
PagI TCATGA 2 cut(s) 969, 1251
PciI ACATGT 1 cut(s) 891
PciSI GCTCTTC 1 cut(s) 981
PcsI WCGNNNNNNNCGW 1 cut(s) 413
PctI GAATGC 1 cut(s) 632
PfeI GAWTC 3 cut(s) 717, 976, 1118
PflMI CCANNNNNTGG 3 cut(s) 125, 175, 730
PkrI GCNGC 3 cut(s) 149, 253, 441
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 408
PscI ACATGT 1 cut(s) 891
Psp124BI GAGCTC 2 cut(s) 951, 993
Psp6I CCWGG 2 cut(s) 100, 306
PspEI GGTNACC 1 cut(s) 128
PspGI CCWGG 2 cut(s) 100, 306
PspN4I GGNNCC 1 cut(s) 1203
PspPI GGNCC 9 cut(s) 104, 191, 297, 362, 665, 748, 936, 1012, 1267
PstI CTGCAG 1 cut(s) 256
PstNI CAGNNNCTG 1 cut(s) 475
PsuI RGATCY 2 cut(s) 1201, 1393
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
SacI GAGCTC 2 cut(s) 951, 993
SacII CCGCGG 1 cut(s) 440
SapI GCTCTTC 1 cut(s) 981
SaqAI TTAA 6 cut(s) 698, 759, 827, 848, 1068, 1386
SatI GCNGC 3 cut(s) 148, 252, 440
Sau3AI GATC 4 cut(s) 859, 1170, 1201, 1393
Sau96I GGNCC 9 cut(s) 104, 191, 297, 362, 665, 748, 936, 1012, 1267
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 4 cut(s) 102, 308, 1274, 1326
SduI GDGCHC 3 cut(s) 951, 993, 1380
SfaNI GCATC 3 cut(s) 888, 911, 967
SfcI CTRYAG 4 cut(s) 63, 252, 999, 1505
Sfr303I CCGCGG 1 cut(s) 440
SgrBI CCGCGG 1 cut(s) 440
SinI GGWCC 6 cut(s) 191, 665, 748, 936, 1012, 1267
SmlI CTYRAG 2 cut(s) 952, 1416
SmoI CTYRAG 2 cut(s) 952, 1416
SsiI CCGC 4 cut(s) 225, 437, 439, 1168
SspMI CTAG 3 cut(s) 143, 1106, 1358
SstI GAGCTC 2 cut(s) 951, 993
StyD4I CCNGG 4 cut(s) 100, 306, 1272, 1324
StyI CCWWGG 1 cut(s) 962
TaaI ACNGT 8 cut(s) 67, 161, 332, 825, 922, 1000, 1351, 1493
TaiI ACGT 4 cut(s) 410, 807, 873, 1185
TaqI TCGA 4 cut(s) 416, 720, 862, 1125
TaqII GACCGA 3 cut(s) 99, 653, 1029
TauI GCSGC 1 cut(s) 442
TfiI GAWTC 3 cut(s) 717, 976, 1118
Tru1I TTAA 6 cut(s) 698, 759, 827, 848, 1068, 1386
Tru9I TTAA 6 cut(s) 698, 759, 827, 848, 1068, 1386
TscAI CASTG 4 cut(s) 323, 480, 769, 1385
TseFI GTSAC 3 cut(s) 111, 471, 515
TseI GCWGC 2 cut(s) 147, 251
Tsp45I GTSAC 3 cut(s) 111, 471, 515
TspDTI ATGAA 8 cut(s) 34, 125, 197, 768, 986, 1131, 1240, 1268
TspRI CASTG 4 cut(s) 323, 480, 769, 1385
Van91I CCANNNNNTGG 3 cut(s) 125, 175, 730
VneI GTGCAC 1 cut(s) 1376
VpaK11BI GGWCC 6 cut(s) 191, 665, 748, 936, 1012, 1267
XapI RAATTY 2 cut(s) 420, 1232
XceI RCATGY 1 cut(s) 895
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XmiI GTMKAC 1 cut(s) 801
XspI CTAG 3 cut(s) 143, 1106, 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.