Rw5G035740

P-loop NTPase domain-containing protein LPA1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004419
Physical Location & Seq
Reverse (-)
62168996 .. 62176420
7425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035740.1

Sequence Viewer

Length: 2106 bp
ATGAGTGAGTCGGCGAAGGTTTTGTACATAGTGGTGGTGGATGGCGAGGAAGCGAGAGAGAAAGGGAAGGAGTCTTTTCGATATACGCGTCCGGTTTTGCAGAGCTCTCTGCAACTCATGGGATGCAAACCTCGACACGCCTTCAAGATTAGTCAACGAGTTTTTGAATTGATAAGAAACGTAAGTTCATGTAATGCCTTGCTTCCGGACGGGACAGAGAATGTTTCCCCTAGTAAAGCTGAGGCTTGCAAGCTTTCAGGGAAAGATCATAGAAGTAAGAGTGTACCATTTGAAATGTACAAAACACGTACGACTGTTGTAGTTAGGAGAGAAACTTACTTGGATGTTGTTTGTGATGCTCTGGCTGAGTACAAGTATGTTGGTCCAAATCAGAGGGCAGACTTAGTTTTGGCATGCAGAATTCGAGAACGGAAGGAATCTGTAACAGTACTGTTGTGCGGCACCAGTGGCTGTGGAAAATCTACTTTGTCTTCATTGCTGGGTAGTAGGTTGGGAATCACAACTGTGATATCTACCGACTCGATTCGGCACATGATGAGGAGTTTCGCAGATGAGAAGCAAAATCCTTTACTATGGGCTTCAACCTATCATGCAGGGGAGTGTTTAGATGCAGTGGCAGTTGCAGAAGCAAAAGCCAAAAAGAAAGCCAAAAAATCAGCTGGAAGTTCACATTCACTTTCTAAAGATGGAATAGTTGATGGCTCTATGTCAGACGCTGGTTCGAGTACTGCTGAATTGATCGGTCCAAAGCAGATGGCCATTGAAGGATTCAAGGCACAAAGTGAGATGGTAATTGACAGTCTTGACCGTCTTATTACTGCATGGGAGGAAAGAAAAGAATCAGTTATTGTAGAAGGTGTTCACTTAAGCCTTAATTTTGTGATGGGGCTTATGAAGAAACACCCTTCGATCATACCATTCATGATATTTATCACAAACGAGGATAAGCACTTGGAGAGATTTGCAGTACGTGCAAAGTATATGACACTGGACCCAGCAAAGAACAAATATGTGAAGTACATCCGAAACATCAGAACAATTCAAGAATATCTCTGCCAGAGGGCTGACAAGCATCTTGTCCCTAAAATTAACAACACTAATGTTGACAAGAGTGTGGCTGCAATCCATGCAACAGTCTTTAGCTGCCTCCGTAGGCGTGAAGCAGGGGAACAACTTTATGATCCCACGAGAAATACAGTAACTGTAGTGGATGAGGAGTATAGGAACCAGCGTGCAGCCAATTCTTTGAGTTCAAAGGGGATGTTTCAACTTATCCAGAGAAAAGGGTCATCTAGGCATCTGATGGCTCTTGTAAATACTGATGGATCTGTAGCAAAGGCTTGGCCTGTTGATTCAGTCGATCGTAATGGGAAGCCTATATTCTGCCATGGGACTGAGATGGAGATTGGCAGTGCAGAGCAAGTGAATCTTCAGTTTGGTCTCTATGGTTTAAGCGCTTGGCCCAAAGATGGTGGCCCAAGCTGTGCTGGAAGTGTTGATGGGTCAAGGGCTGATGATACTGAGACTGTAAGTAGGCATTTCTCTTCGTGCTGCAGCTCACCACGATCGGAGGGACCTGCCAAGGAGCTGAAGGAGGACAACTCGGTGCATGGCAGCGATGAAGAGGTTGATGAATCAGCTGATGTAGGCAGTGATGAGGAATTAAGTGATGATGATGACAAACAAGTTGAAGAAGAGATAGGGTCAGTGGATGAGGAGTCTACGAAATCAGATGAAGAGTATGAAGACCTGGCAATGCAGGATGTTCAGGGGAATGGATACTGGTCAGATGATGGTGCTACGGAGCGCAAGTTTAAGATATTCCCTGTTGCTGAGCACCCATCAGCCAATAAGGATGTGGATAAGTATCGCCAGAACCTGGATCTTTTCCTTAGAACTAGAAACGAGTCATTTTCTGAACCACTATGTTCATATACTTCTCTCTTTATGGAGAAGAATGAGATGACAATGCCGTGCTCTGGCAATATGAAAATTAGAAAACGCCGGTCCCTTAGCATTCCAGCATTGGGAAGGCATGGGTCAAAAACAAGGGGTCCCATACTTTCTGGTGCCCCTCAGTGCTAA

Protein Analysis

701

Amino Acids

77.73

Weight (kDa)

6.4

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_33 PF13671 150 - 214 5.2e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1606
AccB1I GGYRCC 2 cut(s) 461, 2090
AccB7I CCANNNNNTGG 1 cut(s) 1900
AccI GTMKAC 1 cut(s) 1742
AccII CGCG 1 cut(s) 88
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 1 cut(s) 459
AclWI GGATC 3 cut(s) 1196, 1354, 1911
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 1 cut(s) 420
AcuI CTGAAG 2 cut(s) 1436, 1631
AdeI CACNNNGTG 1 cut(s) 803
AfaI GTAC 9 cut(s) 26, 285, 299, 310, 371, 450, 748, 990, 1040
AfeI AGCGCT 1 cut(s) 1477
AfiI CCNNNNNNNGG 4 cut(s) 1490, 1900, 2000, 2048
AflII CTTAAG 1 cut(s) 886
AflIII ACRYGT 2 cut(s) 86, 305
AjnI CCWGG 2 cut(s) 1770, 1899
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 9 cut(s) 105, 239, 253, 680, 1164, 1503, 1578, 1609, 1661
AluI AGCT 9 cut(s) 105, 239, 253, 680, 1164, 1503, 1578, 1609, 1661
Alw21I GWGCWC 3 cut(s) 107, 1860, 2000
Alw26I GTCTC 2 cut(s) 1466, 1538
AlwI GGATC 3 cut(s) 1196, 1354, 1911
AlwNI CAGNNNCTG 4 cut(s) 471, 737, 1223, 1900
Aor13HI TCCGGA 1 cut(s) 205
Aor51HI AGCGCT 1 cut(s) 1477
AoxI GGCC 4 cut(s) 777, 1364, 1481, 1495
ApeKI GCWGC 6 cut(s) 1139, 1164, 1256, 1572, 1575, 1635
ApoI RAATTY 1 cut(s) 420
Asp700I GAANNNNTTC 1 cut(s) 879
AspLEI GCGC 2 cut(s) 1478, 1830
AspS9I GGNCC 8 cut(s) 383, 764, 1012, 1482, 1496, 1595, 2028, 2075
AsuHPI GGTGA 1 cut(s) 1572
AvaII GGWCC 6 cut(s) 383, 764, 1012, 1595, 2028, 2075
BaeGI GKGCMC 1 cut(s) 2095
BalI TGGCCA 1 cut(s) 779
BanI GGYRCC 2 cut(s) 461, 2090
BanII GRGCYC 1 cut(s) 107
BarI GAAGNNNNNNTAC 2 cut(s) 8, 40
BauI CACGAG 1 cut(s) 1207
BbsI GAAGAC 2 cut(s) 483, 1773
Bbv12I GWGCWC 3 cut(s) 107, 1860, 2000
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 6 cut(s) 1126, 1151, 1268, 1559, 1587, 1647
BceAI ACGGC 1 cut(s) 1978
BciT130I CCWGG 2 cut(s) 1772, 1901
BciVI GTATCC 1 cut(s) 1793
BcoDI GTCTC 2 cut(s) 1466, 1538
BfaI CTAG 3 cut(s) 231, 1314, 1920
BfmI CTRYAG 3 cut(s) 1224, 1350, 1573
BfoI RGCGCY 1 cut(s) 1479
BfrI CTTAAG 1 cut(s) 886
BfuAI ACCTGC 1 cut(s) 1606
BfuI GTATCC 1 cut(s) 1793
BisI GCNGC 7 cut(s) 460, 1140, 1165, 1257, 1573, 1576, 1636
BlpI GCTNAGC 1 cut(s) 1854
BlsI GCNGC 7 cut(s) 461, 1141, 1166, 1258, 1574, 1577, 1637
BmcAI AGTACT 2 cut(s) 450, 748
Bme1390I CCNGG 2 cut(s) 1772, 1901
Bme18I GGWCC 6 cut(s) 383, 764, 1012, 1595, 2028, 2075
BmgT120I GGNCC 8 cut(s) 383, 764, 1012, 1482, 1496, 1595, 2028, 2075
BmiI GGNNCC 8 cut(s) 463, 1014, 1247, 1596, 2030, 2076, 2077, 2092
BmrFI CCNGG 2 cut(s) 1772, 1901
BmsI GCATC 5 cut(s) 113, 346, 619, 1102, 1327
BpiI GAAGAC 2 cut(s) 483, 1773
BplI GAGNNNNNCTC 2 cut(s) 1607, 1639
Bpu10I CCTNAGC 2 cut(s) 240, 2033
Bpu1102I GCTNAGC 1 cut(s) 1854
BsaAI YACGTR 2 cut(s) 308, 992
BsaBI GATNNNNATC 2 cut(s) 950, 1887
BsaI GGTCTC 1 cut(s) 1466
BsaJI CCNNGG 2 cut(s) 1408, 1602
BsaWI WCCGGW 2 cut(s) 91, 205
BsaXI ACNNNNNCTCC 2 cut(s) 1817, 1847
Bsc4I CCNNNNNNNGG 4 cut(s) 1490, 1900, 2000, 2048
Bse118I RCCGGY 1 cut(s) 2025
Bse1I ACTGG 3 cut(s) 465, 1014, 1808
Bse3DI GCAATG 2 cut(s) 494, 1782
Bse8I GATNNNNATC 2 cut(s) 950, 1887
BseAI TCCGGA 1 cut(s) 205
BseBI CCWGG 2 cut(s) 1772, 1901
BseDI CCNNGG 2 cut(s) 1408, 1602
BseGI GGATG 9 cut(s) 46, 128, 349, 1041, 1237, 1287, 1738, 1789, 1882
BseJI GATNNNNATC 2 cut(s) 950, 1887
BseLI CCNNNNNNNGG 4 cut(s) 1490, 1900, 2000, 2048
BseMI GCAATG 2 cut(s) 494, 1782
BseMII CTCAG 5 cut(s) 231, 357, 1407, 1533, 1845
BseNI ACTGG 3 cut(s) 465, 1014, 1808
BseRI GAGGAG 3 cut(s) 574, 1250, 1751
BseSI GKGCMC 1 cut(s) 2095
BseXI GCAGC 6 cut(s) 1126, 1151, 1268, 1559, 1587, 1647
BseYI CCCAGC 2 cut(s) 499, 1015
BsgI GTGCAG 2 cut(s) 1275, 1455
Bsh1236I CGCG 1 cut(s) 88
Bsh1285I CGRYCG 2 cut(s) 1384, 1589
BshFI GGCC 4 cut(s) 779, 1366, 1483, 1497
BshNI GGYRCC 2 cut(s) 461, 2090
BsiEI CGRYCG 2 cut(s) 1384, 1589
BsiHKAI GWGCWC 3 cut(s) 107, 1860, 2000
BsiSI CCGG 3 cut(s) 92, 206, 2026
BsiWI CGTACG 1 cut(s) 308
BslFI GGGAC 6 cut(s) 226, 1085, 1426, 1608, 2014, 2061
BslI CCNNNNNNNGG 4 cut(s) 1490, 1900, 2000, 2048
BsmAI GTCTC 2 cut(s) 1466, 1538
BsmFI GGGAC 6 cut(s) 226, 1085, 1426, 1608, 2014, 2061
BsmI GAATGC 1 cut(s) 2037
BsnI GGCC 4 cut(s) 779, 1366, 1483, 1497
Bso31I GGTCTC 1 cut(s) 1466
Bsp1286I GDGCHC 4 cut(s) 107, 1860, 2000, 2095
Bsp13I TCCGGA 1 cut(s) 205
Bsp1407I TGTACA 2 cut(s) 24, 297
Bsp143I GATC 8 cut(s) 265, 759, 930, 1201, 1346, 1381, 1586, 1903
Bsp1720I GCTNAGC 1 cut(s) 1854
Bsp19I CCATGG 1 cut(s) 1408
BspACI CCGC 1 cut(s) 459
BspANI GGCC 4 cut(s) 779, 1366, 1483, 1497
BspCNI CTCAG 5 cut(s) 232, 358, 1408, 1534, 1846
BspEI TCCGGA 1 cut(s) 205
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 1 cut(s) 942
BspLI GGNNCC 8 cut(s) 463, 1014, 1247, 1596, 2030, 2076, 2077, 2092
BspMAI CTGCAG 1 cut(s) 1577
BspMI ACCTGC 1 cut(s) 1606
BspPI GGATC 3 cut(s) 1196, 1354, 1911
BspT107I GGYRCC 2 cut(s) 461, 2090
BspTI CTTAAG 1 cut(s) 886
BspTNI GGTCTC 1 cut(s) 1466
BsrDI GCAATG 2 cut(s) 494, 1782
BsrFI RCCGGY 1 cut(s) 2025
BsrGI TGTACA 2 cut(s) 24, 297
BsrI ACTGG 3 cut(s) 465, 1014, 1808
BssAI RCCGGY 1 cut(s) 2025
BssECI CCNNGG 2 cut(s) 1408, 1602
BssMI GATC 8 cut(s) 265, 759, 930, 1201, 1346, 1381, 1586, 1903
BssSI CACGAG 1 cut(s) 1207
BssT1I CCWWGG 2 cut(s) 1408, 1602
Bst2BI CACGAG 1 cut(s) 1207
Bst2UI CCWGG 2 cut(s) 1772, 1901
Bst6I CTCTTC 4 cut(s) 1570, 1638, 1710, 1752
BstAFI CTTAAG 1 cut(s) 886
BstAPI GCANNNNNTGC 2 cut(s) 992, 1148
BstAUI TGTACA 2 cut(s) 24, 297
BstBAI YACGTR 2 cut(s) 308, 992
BstC8I GCNNGC 4 cut(s) 247, 251, 415, 1254
BstDEI CTNAG 9 cut(s) 240, 366, 403, 1416, 1542, 1854, 1913, 2033, 2097
BstDSI CCRYGG 1 cut(s) 1408
BstF5I GGATG 9 cut(s) 46, 128, 349, 1041, 1237, 1287, 1738, 1789, 1882
BstFNI CGCG 1 cut(s) 88
BstH2I RGCGCY 1 cut(s) 1479
BstHHI GCGC 2 cut(s) 1478, 1830
BstKTI GATC 8 cut(s) 268, 762, 933, 1204, 1349, 1384, 1589, 1906
BstMAI GTCTC 2 cut(s) 1466, 1538
BstMBI GATC 8 cut(s) 265, 759, 930, 1201, 1346, 1381, 1586, 1903
BstMCI CGRYCG 2 cut(s) 1384, 1589
BstMWI GCNNNNNNNGC 3 cut(s) 468, 992, 1148
BstNI CCWGG 2 cut(s) 1772, 1901
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 2 cut(s) 1770, 1899
BstSFI CTRYAG 3 cut(s) 1224, 1350, 1573
BstSLI GKGCMC 1 cut(s) 2095
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 6 cut(s) 1126, 1151, 1268, 1559, 1587, 1647
BstV2I GAAGAC 2 cut(s) 483, 1773
BstX2I RGATCY 2 cut(s) 1346, 1903
BstYI RGATCY 2 cut(s) 1346, 1903
BsuI GTATCC 1 cut(s) 1793
BsuRI GGCC 4 cut(s) 779, 1366, 1483, 1497
BtgI CCRYGG 1 cut(s) 1408
BtgZI GCGATG 1 cut(s) 1653
BtsCI GGATG 9 cut(s) 46, 128, 349, 1041, 1237, 1287, 1738, 1789, 1882
BtsI GCAGTG 3 cut(s) 639, 1438, 1678
BtsIMutI CAGTG 7 cut(s) 472, 639, 1007, 1438, 1678, 1734, 2105
BveI ACCTGC 1 cut(s) 1606
Cac8I GCNNGC 4 cut(s) 247, 251, 415, 1254
CaiI CAGNNNCTG 4 cut(s) 471, 737, 1223, 1900
CciI TCATGA 1 cut(s) 942
CfoI GCGC 2 cut(s) 1478, 1830
Cfr10I RCCGGY 1 cut(s) 2025
Cfr13I GGNCC 8 cut(s) 383, 764, 1012, 1482, 1496, 1595, 2028, 2075
CseI GACGC 2 cut(s) 77, 743
Csp6I GTAC 9 cut(s) 25, 284, 298, 309, 370, 449, 747, 989, 1039
CspCI CAANNNNNGTGG 2 cut(s) 1474, 1509
CviQI GTAC 9 cut(s) 25, 284, 298, 309, 370, 449, 747, 989, 1039
DdeI CTNAG 9 cut(s) 240, 366, 403, 1416, 1542, 1854, 1913, 2033, 2097
DpnI GATC 8 cut(s) 267, 761, 932, 1203, 1348, 1383, 1588, 1905
DpnII GATC 8 cut(s) 265, 759, 930, 1201, 1346, 1381, 1586, 1903
DraIII CACNNNGTG 1 cut(s) 803
EaeI YGGCCR 1 cut(s) 777
Eam1104I CTCTTC 4 cut(s) 1570, 1638, 1710, 1752
EarI CTCTTC 4 cut(s) 1570, 1638, 1710, 1752
Ecl136II GAGCTC 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 1408, 1602
Eco24I GRGCYC 1 cut(s) 107
Eco31I GGTCTC 1 cut(s) 1466
Eco32I GATATC 1 cut(s) 531
Eco47I GGWCC 6 cut(s) 383, 764, 1012, 1595, 2028, 2075
Eco47III AGCGCT 1 cut(s) 1477
Eco53kI GAGCTC 1 cut(s) 105
Eco57I CTGAAG 2 cut(s) 1436, 1631
EcoICRI GAGCTC 1 cut(s) 105
EcoO109I RGGNCCY 2 cut(s) 1595, 2075
EcoRI GAATTC 1 cut(s) 420
EcoRII CCWGG 2 cut(s) 1770, 1899
EcoRV GATATC 1 cut(s) 531
EcoT14I CCWWGG 2 cut(s) 1408, 1602
EcoT38I GRGCYC 1 cut(s) 107
ErhI CCWWGG 2 cut(s) 1408, 1602
FalI AAGNNNNNCTT 2 cut(s) 1434, 1466
FaqI GGGAC 6 cut(s) 226, 1085, 1426, 1608, 2014, 2061
FblI GTMKAC 1 cut(s) 1742
Fnu4HI GCNGC 7 cut(s) 460, 1140, 1165, 1257, 1573, 1576, 1636
FokI GGATG 9 cut(s) 53, 135, 356, 1028, 1244, 1294, 1745, 1796, 1889
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 7 cut(s) 460, 1140, 1165, 1257, 1573, 1576, 1636
FspBI CTAG 3 cut(s) 231, 1314, 1920
GlaI GCGC 2 cut(s) 1477, 1829
GluI GCNGC 7 cut(s) 460, 1140, 1165, 1257, 1573, 1576, 1636
GsaI CCCAGC 2 cut(s) 503, 1019
HaeII RGCGCY 1 cut(s) 1479
HaeIII GGCC 4 cut(s) 779, 1366, 1483, 1497
HapII CCGG 3 cut(s) 92, 206, 2026
HgaI GACGC 2 cut(s) 77, 743
HhaI GCGC 2 cut(s) 1478, 1830
Hin6I GCGC 2 cut(s) 1476, 1828
HinP1I GCGC 2 cut(s) 1476, 1828
HincII GTYRAC 2 cut(s) 155, 1126
HindII GTYRAC 2 cut(s) 155, 1126
HindIII AAGCTT 1 cut(s) 251
HpaII CCGG 3 cut(s) 92, 206, 2026
HphI GGTGA 1 cut(s) 1572
Hpy166II GTNNAC 6 cut(s) 155, 284, 689, 883, 1126, 1743
Hpy188I TCNGA 9 cut(s) 393, 733, 1046, 1055, 1323, 1591, 1753, 1810, 1939
Hpy188III TCNNGA 7 cut(s) 145, 206, 425, 824, 943, 1064, 1297
Hpy8I GTNNAC 6 cut(s) 155, 284, 689, 883, 1126, 1743
HpyAV CCTTC 9 cut(s) 10, 61, 151, 427, 779, 869, 936, 1606, 2046
HpyCH4IV ACGT 3 cut(s) 180, 307, 991
HpyF10VI GCNNNNNNNGC 3 cut(s) 468, 992, 1148
HpyF3I CTNAG 9 cut(s) 240, 366, 403, 1416, 1542, 1854, 1913, 2033, 2097
HpySE526I ACGT 3 cut(s) 180, 307, 991
HspAI GCGC 2 cut(s) 1476, 1828
KflI GGGWCCC 1 cut(s) 2075
Kpn2I TCCGGA 1 cut(s) 205
Kzo9I GATC 8 cut(s) 265, 759, 930, 1201, 1346, 1381, 1586, 1903
LmnI GCTCC 2 cut(s) 1606, 1825
Lsp1109I GCAGC 6 cut(s) 1126, 1151, 1268, 1559, 1587, 1647
LweI GCATC 5 cut(s) 113, 346, 619, 1102, 1327
MaeI CTAG 3 cut(s) 231, 1314, 1920
MaeII ACGT 3 cut(s) 180, 307, 991
MaeIII GTNAC 2 cut(s) 442, 1219
MalI GATC 8 cut(s) 267, 761, 932, 1203, 1348, 1383, 1588, 1905
MboI GATC 8 cut(s) 265, 759, 930, 1201, 1346, 1381, 1586, 1903
MflI RGATCY 2 cut(s) 1346, 1903
MhlI GDGCHC 4 cut(s) 107, 1860, 2000, 2095
MlsI TGGCCA 1 cut(s) 779
MluI ACGCGT 1 cut(s) 86
MluNI TGGCCA 1 cut(s) 779
MlyI GAGTC 5 cut(s) 17, 80, 533, 1748, 1937
Mox20I TGGCCA 1 cut(s) 779
MroI TCCGGA 1 cut(s) 205
MroXI GAANNNNTTC 1 cut(s) 879
MscI TGGCCA 1 cut(s) 779
MseI TTAA 6 cut(s) 887, 894, 1110, 1472, 1685, 1836
MslI CAYNNNNRTG 2 cut(s) 32, 524
Msp20I TGGCCA 1 cut(s) 779
MspA1I CMGCKG 2 cut(s) 680, 1661
MspCI CTTAAG 1 cut(s) 886
MspI CCGG 3 cut(s) 92, 206, 2026
MspR9I CCNGG 2 cut(s) 1772, 1901
Mva1269I GAATGC 1 cut(s) 2037
MvaI CCWGG 2 cut(s) 1772, 1901
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 3 cut(s) 468, 992, 1148
NcoI CCATGG 1 cut(s) 1408
NdeII GATC 8 cut(s) 265, 759, 930, 1201, 1346, 1381, 1586, 1903
NlaIV GGNNCC 8 cut(s) 463, 1014, 1247, 1596, 2030, 2076, 2077, 2092
NspI RCATGY 1 cut(s) 417
OliI CACNNNNGTG 1 cut(s) 524
PaeI GCATGC 1 cut(s) 417
PagI TCATGA 1 cut(s) 942
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PctI GAATGC 1 cut(s) 2037
PdmI GAANNNNTTC 1 cut(s) 879
PfeI GAWTC 8 cut(s) 437, 516, 544, 789, 860, 1373, 1447, 1655
Pfl23II CGTACG 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 1900
PkrI GCNGC 7 cut(s) 461, 1141, 1166, 1258, 1574, 1577, 1637
Ple19I CGATCG 2 cut(s) 1384, 1589
PleI GAGTC 5 cut(s) 16, 79, 533, 1747, 1936
PpsI GAGTC 5 cut(s) 16, 79, 533, 1747, 1936
Ppu21I YACGTR 2 cut(s) 308, 992
PpuMI RGGWCCY 2 cut(s) 1595, 2075
Psp124BI GAGCTC 1 cut(s) 107
Psp5II RGGWCCY 2 cut(s) 1595, 2075
Psp6I CCWGG 2 cut(s) 1770, 1899
PspFI CCCAGC 2 cut(s) 499, 1015
PspGI CCWGG 2 cut(s) 1770, 1899
PspLI CGTACG 1 cut(s) 308
PspN4I GGNNCC 8 cut(s) 463, 1014, 1247, 1596, 2030, 2076, 2077, 2092
PspPI GGNCC 8 cut(s) 383, 764, 1012, 1482, 1496, 1595, 2028, 2075
PspPPI RGGWCCY 2 cut(s) 1595, 2075
PsrI GAACNNNNNNTAC 2 cut(s) 864, 896
PstI CTGCAG 1 cut(s) 1577
PstNI CAGNNNCTG 4 cut(s) 471, 737, 1223, 1900
PsuI RGATCY 2 cut(s) 1346, 1903
PvuI CGATCG 2 cut(s) 1384, 1589
PvuII CAGCTG 2 cut(s) 680, 1661
RsaI GTAC 9 cut(s) 26, 285, 299, 310, 371, 450, 748, 990, 1040
RsaNI GTAC 9 cut(s) 25, 284, 298, 309, 370, 449, 747, 989, 1039
RseI CAYNNNNRTG 2 cut(s) 32, 524
SacI GAGCTC 1 cut(s) 107
SaqAI TTAA 6 cut(s) 887, 894, 1110, 1472, 1685, 1836
SatI GCNGC 7 cut(s) 460, 1140, 1165, 1257, 1573, 1576, 1636
Sau3AI GATC 8 cut(s) 265, 759, 930, 1201, 1346, 1381, 1586, 1903
Sau96I GGNCC 8 cut(s) 383, 764, 1012, 1482, 1496, 1595, 2028, 2075
ScaI AGTACT 2 cut(s) 450, 748
SchI GAGTC 5 cut(s) 17, 80, 533, 1748, 1937
ScrFI CCNGG 2 cut(s) 1772, 1901
SduI GDGCHC 4 cut(s) 107, 1860, 2000, 2095
SfaNI GCATC 5 cut(s) 113, 346, 619, 1102, 1327
SfcI CTRYAG 3 cut(s) 1224, 1350, 1573
SinI GGWCC 6 cut(s) 383, 764, 1012, 1595, 2028, 2075
SmiMI CAYNNNNRTG 2 cut(s) 32, 524
SmlI CTYRAG 1 cut(s) 886
SmoI CTYRAG 1 cut(s) 886
SphI GCATGC 1 cut(s) 417
SsiI CCGC 1 cut(s) 459
SspMI CTAG 3 cut(s) 231, 1314, 1920
SstI GAGCTC 1 cut(s) 107
StyD4I CCNGG 2 cut(s) 1770, 1899
StyI CCWWGG 2 cut(s) 1408, 1602
TaiI ACGT 3 cut(s) 183, 310, 994
TaqI TCGA 7 cut(s) 79, 133, 424, 542, 743, 929, 1380
TaqII GACCGA 1 cut(s) 752
TatI WGTACW 6 cut(s) 24, 297, 369, 448, 746, 1038
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 8 cut(s) 437, 516, 544, 789, 860, 1373, 1447, 1655
Tru1I TTAA 6 cut(s) 887, 894, 1110, 1472, 1685, 1836
Tru9I TTAA 6 cut(s) 887, 894, 1110, 1472, 1685, 1836
TscAI CASTG 7 cut(s) 472, 639, 1014, 1438, 1678, 1734, 2105
TseI GCWGC 6 cut(s) 1139, 1164, 1256, 1572, 1575, 1635
TspGWI ACGGA 3 cut(s) 445, 1160, 1838
TspRI CASTG 7 cut(s) 472, 639, 1014, 1438, 1678, 1734, 2105
Van91I CCANNNNNTGG 1 cut(s) 1900
Vha464I CTTAAG 1 cut(s) 886
VpaK11BI GGWCC 6 cut(s) 383, 764, 1012, 1595, 2028, 2075
XapI RAATTY 1 cut(s) 420
XceI RCATGY 1 cut(s) 417
XcmI CCANNNNNNNNNTGG 1 cut(s) 1876
XmiI GTMKAC 1 cut(s) 1742
XmnI GAANNNNTTC 1 cut(s) 879
XspI CTAG 3 cut(s) 231, 1314, 1920
ZrmI AGTACT 2 cut(s) 450, 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.