Rh7BG226900

P-loop NTPase domain-containing protein LPA1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004419
Physical Location & Seq
Reverse (-)
19567482 .. 19570600
3119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG226900.1

Sequence Viewer

Length: 1257 bp
ATGGTTTCAGGGAAAGATCATAGATGTAAGAGTGTACCATTTGAAATGTACAAAACACGTACGACTGTTGTAATTAGGAGAGAAACTTACTTGGATGTTGTTTGTGATGCTCTGGCTAAGTACAAGTATGTTGGTCCAAATCAGAGGGCAGACTTTGTTTTGGCATGCAGAATTCGAGAACGGAAGGAATCTGTAACAGTACTGTTGTGCGGCACCAGTGGCTGTGGAAAATCTACTTTGTCTTCATTGCTGGGTAGTAGGTTGGGAATCACAACTGTGATATCTACCGACTCGATTCGGCACATGATGAGGAGTTTCGCAGATGAGAAGCAAAATCCTTTACTATGGGCTTCAACCTATCATGCAGGGGAGTGTTTAGATCCAGTGGCAGTTGCAGAAGCAAAAGCCAAAAAGAAAGCCAAAAAATCAGCTGGAAGTTCACATTCACTTTCTAAAGATGGAATAATTGATGGCTCTATGTCAGACACTGGTTCGAGTACTGCTGAATTGATCGGTCCAAAGCAGATGGCCATTGAAGGATTCAAGGCACAAAGTGAGATGGTAATTGACAGTCTTGACCGTCTTATTACTGCATGGGAGGAAAGAAAAGAATCAGTTATTGTAGAAGGTGTTCACTTAAGCCTTAATTTTGTGATGGGGCTTATGAAGAAACACCCTTCGATCATACCATTCATGATATTTATCACAAATGAGGATAAGCACTTGGAGAGATTTGCAGTACGTGCAAAGTATATGACACTGGACCCAGCAAAGAACAAATATGTGAAGTACATCCGAAACATAAGAACAATTCAAGAATATCTCTGCCAGAGGGCTGACAAGCATCTTGTCCCCAAAATTAACAACACTAATGTTGACAAGAGTGTAGCTGCAATCCATGCAACAGTCTTTAGCTGCCTCCGTAGGCGTGAAGCAGGGGAACAACTTTATGATCCCACGAGAAATACAGTAACCGTAGTGGATGAGGAGTATAGGAACCAGCGTGCAGCCAATTCTTTGAGTTCAAAGGGGATGTTTCAACTTATCCAGAGAAAAGGGTCATCTAGGCATCTGATGGCTCTTGTAAATACTGATGGATCTGTTGCAAAGGCTTGGCCTGTTGATTCAGTCGATCGTAATGGGAAGCCTATATTCTGCCATGGGACTGAGATGGAGATTGGCAGTGCAGAGCAAGTGAATCTTCAGTTTGGTCTCTATGGTTTAAGCGCTTGGCCCAAGCTGTGCTGGAAGTGTTGA

Protein Analysis

418

Amino Acids

46.73

Weight (kDa)

9.3

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_33 PF13671 67 - 113 3.2e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AciI CCGC 1 cut(s) 210
AclWI GGATC 3 cut(s) 374, 947, 1105
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 1187
AdeI CACNNNGTG 1 cut(s) 554
AfaI GTAC 8 cut(s) 36, 50, 61, 122, 201, 499, 741, 791
AfeI AGCGCT 1 cut(s) 1228
AflII CTTAAG 1 cut(s) 637
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 7 cut(s) 44, 354, 536, 544, 815, 1026, 1040
AleI CACNNNNGTG 1 cut(s) 275
AluBI AGCT 4 cut(s) 431, 890, 915, 1240
AluI AGCT 4 cut(s) 431, 890, 915, 1240
Alw26I GTCTC 1 cut(s) 1217
AlwI GGATC 3 cut(s) 374, 947, 1105
AlwNI CAGNNNCTG 2 cut(s) 222, 488
Aor51HI AGCGCT 1 cut(s) 1228
AoxI GGCC 3 cut(s) 528, 1115, 1232
ApeKI GCWGC 3 cut(s) 890, 915, 1007
ApoI RAATTY 1 cut(s) 171
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 1 cut(s) 1229
AspS9I GGNCC 4 cut(s) 134, 515, 763, 1233
AvaII GGWCC 3 cut(s) 134, 515, 763
BalI TGGCCA 1 cut(s) 530
BanI GGYRCC 1 cut(s) 212
BauI CACGAG 1 cut(s) 958
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 877, 902, 1019
BccI CCATC 8 cut(s) 452, 464, 520, 553, 649, 1069, 1088, 1165
BcoDI GTCTC 1 cut(s) 1217
BfaI CTAG 1 cut(s) 1065
BfoI RGCGCY 1 cut(s) 1230
BfrI CTTAAG 1 cut(s) 637
BisI GCNGC 4 cut(s) 211, 891, 916, 1008
BlsI GCNGC 4 cut(s) 212, 892, 917, 1009
BmcAI AGTACT 2 cut(s) 201, 499
Bme18I GGWCC 3 cut(s) 134, 515, 763
BmgT120I GGNCC 4 cut(s) 134, 515, 763, 1233
BmiI GGNNCC 3 cut(s) 214, 765, 998
BmsI GCATC 3 cut(s) 97, 853, 1078
BpiI GAAGAC 1 cut(s) 234
BsaAI YACGTR 2 cut(s) 59, 743
BsaBI GATNNNNATC 1 cut(s) 701
BsaI GGTCTC 1 cut(s) 1217
BsaJI CCNNGG 1 cut(s) 1159
Bse1I ACTGG 4 cut(s) 216, 383, 493, 765
Bse3DI GCAATG 1 cut(s) 245
Bse8I GATNNNNATC 1 cut(s) 701
BseDI CCNNGG 1 cut(s) 1159
BseGI GGATG 4 cut(s) 100, 792, 988, 1038
BseJI GATNNNNATC 1 cut(s) 701
BseMI GCAATG 1 cut(s) 245
BseMII CTCAG 1 cut(s) 1158
BseNI ACTGG 4 cut(s) 216, 383, 493, 765
BseRI GAGGAG 2 cut(s) 325, 1001
BseXI GCAGC 3 cut(s) 877, 902, 1019
BseYI CCCAGC 2 cut(s) 250, 766
BsgI GTGCAG 2 cut(s) 1026, 1206
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 3 cut(s) 530, 1117, 1234
BshNI GGYRCC 1 cut(s) 212
BsiEI CGRYCG 1 cut(s) 1135
BsiWI CGTACG 1 cut(s) 59
BslFI GGGAC 2 cut(s) 836, 1177
BsmAI GTCTC 1 cut(s) 1217
BsmFI GGGAC 2 cut(s) 836, 1177
BsnI GGCC 3 cut(s) 530, 1117, 1234
Bso31I GGTCTC 1 cut(s) 1217
Bsp1407I TGTACA 1 cut(s) 48
Bsp143I GATC 7 cut(s) 16, 379, 510, 681, 952, 1097, 1132
Bsp19I CCATGG 1 cut(s) 1159
BspACI CCGC 1 cut(s) 210
BspANI GGCC 3 cut(s) 530, 1117, 1234
BspCNI CTCAG 1 cut(s) 1159
BspHI TCATGA 1 cut(s) 693
BspLI GGNNCC 3 cut(s) 214, 765, 998
BspPI GGATC 3 cut(s) 374, 947, 1105
BspT107I GGYRCC 1 cut(s) 212
BspTI CTTAAG 1 cut(s) 637
BspTNI GGTCTC 1 cut(s) 1217
BsrDI GCAATG 1 cut(s) 245
BsrGI TGTACA 1 cut(s) 48
BsrI ACTGG 4 cut(s) 216, 383, 493, 765
BssECI CCNNGG 1 cut(s) 1159
BssMI GATC 7 cut(s) 16, 379, 510, 681, 952, 1097, 1132
BssSI CACGAG 1 cut(s) 958
BssT1I CCWWGG 1 cut(s) 1159
Bst2BI CACGAG 1 cut(s) 958
Bst4CI ACNGT 9 cut(s) 67, 199, 204, 277, 572, 581, 907, 970, 976
BstAFI CTTAAG 1 cut(s) 637
BstAPI GCANNNNNTGC 2 cut(s) 743, 899
BstAUI TGTACA 1 cut(s) 48
BstBAI YACGTR 2 cut(s) 59, 743
BstC8I GCNNGC 2 cut(s) 166, 1005
BstDEI CTNAG 2 cut(s) 117, 1167
BstDSI CCRYGG 1 cut(s) 1159
BstF5I GGATG 4 cut(s) 100, 792, 988, 1038
BstH2I RGCGCY 1 cut(s) 1230
BstHHI GCGC 1 cut(s) 1229
BstKTI GATC 7 cut(s) 19, 382, 513, 684, 955, 1100, 1135
BstMAI GTCTC 1 cut(s) 1217
BstMBI GATC 7 cut(s) 16, 379, 510, 681, 952, 1097, 1132
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 3 cut(s) 219, 743, 899
BstNSI RCATGY 1 cut(s) 168
BstV1I GCAGC 3 cut(s) 877, 902, 1019
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 2 cut(s) 379, 1097
BstYI RGATCY 2 cut(s) 379, 1097
BsuRI GGCC 3 cut(s) 530, 1117, 1234
BtgI CCRYGG 1 cut(s) 1159
BtsCI GGATG 4 cut(s) 100, 792, 988, 1038
BtsI GCAGTG 1 cut(s) 1189
BtsIMutI CAGTG 5 cut(s) 223, 390, 486, 758, 1189
Cac8I GCNNGC 2 cut(s) 166, 1005
CaiI CAGNNNCTG 2 cut(s) 222, 488
CciI TCATGA 1 cut(s) 693
CfoI GCGC 1 cut(s) 1229
Cfr13I GGNCC 4 cut(s) 134, 515, 763, 1233
Csp6I GTAC 8 cut(s) 35, 49, 60, 121, 200, 498, 740, 790
CviAII CATG 7 cut(s) 165, 304, 362, 594, 694, 899, 1160
CviQI GTAC 8 cut(s) 35, 49, 60, 121, 200, 498, 740, 790
DdeI CTNAG 2 cut(s) 117, 1167
DpnI GATC 7 cut(s) 18, 381, 512, 683, 954, 1099, 1134
DpnII GATC 7 cut(s) 16, 379, 510, 681, 952, 1097, 1132
DraIII CACNNNGTG 1 cut(s) 554
EaeI YGGCCR 1 cut(s) 528
Eco130I CCWWGG 1 cut(s) 1159
Eco31I GGTCTC 1 cut(s) 1217
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 3 cut(s) 134, 515, 763
Eco47III AGCGCT 1 cut(s) 1228
Eco57I CTGAAG 1 cut(s) 1187
EcoRI GAATTC 1 cut(s) 171
EcoRV GATATC 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 1159
ErhI CCWWGG 1 cut(s) 1159
FaeI CATG 7 cut(s) 168, 307, 365, 597, 697, 902, 1163
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 2 cut(s) 836, 1177
FatI CATG 7 cut(s) 164, 303, 361, 593, 693, 898, 1159
Fnu4HI GCNGC 4 cut(s) 211, 891, 916, 1008
FokI GGATG 4 cut(s) 107, 779, 995, 1045
Fsp4HI GCNGC 4 cut(s) 211, 891, 916, 1008
FspBI CTAG 1 cut(s) 1065
GlaI GCGC 1 cut(s) 1228
GluI GCNGC 4 cut(s) 211, 891, 916, 1008
GsaI CCCAGC 2 cut(s) 254, 770
HaeII RGCGCY 1 cut(s) 1230
HaeIII GGCC 3 cut(s) 530, 1117, 1234
HhaI GCGC 1 cut(s) 1229
Hin1II CATG 7 cut(s) 168, 307, 365, 597, 697, 902, 1163
Hin6I GCGC 1 cut(s) 1227
HinP1I GCGC 1 cut(s) 1227
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HinfI GANTC 8 cut(s) 188, 267, 290, 295, 540, 611, 1124, 1198
Hpy166II GTNNAC 4 cut(s) 35, 440, 634, 877
Hpy188I TCNGA 4 cut(s) 144, 484, 797, 1074
Hpy188III TCNNGA 5 cut(s) 176, 575, 694, 815, 1048
Hpy8I GTNNAC 4 cut(s) 35, 440, 634, 877
HpyAV CCTTC 4 cut(s) 178, 530, 620, 687
HpyCH4III ACNGT 9 cut(s) 67, 199, 204, 277, 572, 581, 907, 970, 976
HpyCH4IV ACGT 2 cut(s) 58, 742
HpyF10VI GCNNNNNNNGC 3 cut(s) 219, 743, 899
HpyF3I CTNAG 2 cut(s) 117, 1167
HpySE526I ACGT 2 cut(s) 58, 742
Hsp92II CATG 7 cut(s) 168, 307, 365, 597, 697, 902, 1163
HspAI GCGC 1 cut(s) 1227
Kzo9I GATC 7 cut(s) 16, 379, 510, 681, 952, 1097, 1132
Lsp1109I GCAGC 3 cut(s) 877, 902, 1019
LweI GCATC 3 cut(s) 97, 853, 1078
MaeI CTAG 1 cut(s) 1065
MaeII ACGT 2 cut(s) 58, 742
MaeIII GTNAC 2 cut(s) 193, 970
MalI GATC 7 cut(s) 18, 381, 512, 683, 954, 1099, 1134
MboI GATC 7 cut(s) 16, 379, 510, 681, 952, 1097, 1132
MboII GAAGA 3 cut(s) 234, 679, 1193
MflI RGATCY 2 cut(s) 379, 1097
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 9 cut(s) 72, 171, 465, 506, 564, 646, 810, 858, 1012
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 1 cut(s) 284
MnlI CCTC 7 cut(s) 138, 303, 592, 706, 825, 929, 979
Mox20I TGGCCA 1 cut(s) 530
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 1 cut(s) 530
MseI TTAA 4 cut(s) 638, 645, 861, 1223
MslI CAYNNNNRTG 1 cut(s) 275
Msp20I TGGCCA 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 431
MspCI CTTAAG 1 cut(s) 637
MwoI GCNNNNNNNGC 3 cut(s) 219, 743, 899
NcoI CCATGG 1 cut(s) 1159
NdeII GATC 7 cut(s) 16, 379, 510, 681, 952, 1097, 1132
NlaIII CATG 7 cut(s) 168, 307, 365, 597, 697, 902, 1163
NlaIV GGNNCC 3 cut(s) 214, 765, 998
NspI RCATGY 1 cut(s) 168
OliI CACNNNNGTG 1 cut(s) 275
PaeI GCATGC 1 cut(s) 168
PagI TCATGA 1 cut(s) 693
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 7 cut(s) 188, 267, 295, 540, 611, 1124, 1198
Pfl23II CGTACG 1 cut(s) 59
PkrI GCNGC 4 cut(s) 212, 892, 917, 1009
Ple19I CGATCG 1 cut(s) 1135
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
Ppu21I YACGTR 2 cut(s) 59, 743
PspFI CCCAGC 2 cut(s) 250, 766
PspLI CGTACG 1 cut(s) 59
PspN4I GGNNCC 3 cut(s) 214, 765, 998
PspPI GGNCC 4 cut(s) 134, 515, 763, 1233
PsrI GAACNNNNNNTAC 2 cut(s) 615, 647
PstNI CAGNNNCTG 2 cut(s) 222, 488
PsuI RGATCY 2 cut(s) 379, 1097
PvuI CGATCG 1 cut(s) 1135
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 8 cut(s) 36, 50, 61, 122, 201, 499, 741, 791
RsaNI GTAC 8 cut(s) 35, 49, 60, 121, 200, 498, 740, 790
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 4 cut(s) 638, 645, 861, 1223
SatI GCNGC 4 cut(s) 211, 891, 916, 1008
Sau3AI GATC 7 cut(s) 16, 379, 510, 681, 952, 1097, 1132
Sau96I GGNCC 4 cut(s) 134, 515, 763, 1233
ScaI AGTACT 2 cut(s) 201, 499
SchI GAGTC 1 cut(s) 284
SetI ASST 9 cut(s) 61, 263, 359, 433, 631, 745, 892, 917, 1242
SfaNI GCATC 3 cut(s) 97, 853, 1078
SinI GGWCC 3 cut(s) 134, 515, 763
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 637
SmoI CTYRAG 1 cut(s) 637
SphI GCATGC 1 cut(s) 168
Sse9I AATT 9 cut(s) 72, 171, 465, 506, 564, 646, 810, 858, 1012
SsiI CCGC 1 cut(s) 210
SspMI CTAG 1 cut(s) 1065
StyI CCWWGG 1 cut(s) 1159
TaaI ACNGT 9 cut(s) 67, 199, 204, 277, 572, 581, 907, 970, 976
TaiI ACGT 2 cut(s) 61, 745
TaqI TCGA 5 cut(s) 175, 293, 494, 680, 1131
TaqII GACCGA 1 cut(s) 503
TasI AATT 9 cut(s) 72, 171, 465, 506, 564, 646, 810, 858, 1012
TatI WGTACW 5 cut(s) 48, 120, 199, 497, 789
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 7 cut(s) 188, 267, 295, 540, 611, 1124, 1198
Tru1I TTAA 4 cut(s) 638, 645, 861, 1223
Tru9I TTAA 4 cut(s) 638, 645, 861, 1223
TscAI CASTG 5 cut(s) 223, 390, 493, 765, 1189
TseI GCWGC 3 cut(s) 890, 915, 1007
TspDTI ATGAA 3 cut(s) 234, 680, 682
TspGWI ACGGA 2 cut(s) 196, 911
TspRI CASTG 5 cut(s) 223, 390, 493, 765, 1189
Vha464I CTTAAG 1 cut(s) 637
VpaK11BI GGWCC 3 cut(s) 134, 515, 763
XapI RAATTY 1 cut(s) 171
XceI RCATGY 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 1 cut(s) 1065
ZrmI AGTACT 2 cut(s) 201, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.