Rorug06G0265000
MADS Family

Floral homeotic protein PMADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006738
Physical Location & Seq
Reverse (-)
41150243 .. 41153833
3591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0265000.1

Sequence Viewer

Length: 993 bp
ATGTGGACGATGATGGGTAGCAGCAGTGGTTGTAGAGACCCTAATAATAGGGCTCTGCCTGAGTACTCGATCAAAGCTGCATATCCTCAGCCTGCAGTACTACTCACTCCAAAGCTTAGCTCTACTTCCAACATGACCCAAATCCCTTTGTTACCATTCAACCACCACCATCAGATGGTTCCAGTGAGGTCGAATGAGCAAGGCAGAAATAGCAGTGATTGTTCTTATAGTGATAAACAACCGGCAGCTGCAGCTGCTCCGCGAAGATCCCGATGGACTCCAACCCCGGAGCAGATAGTGCATCTGGAAGAGCTGTATCGATCCGGGATCAAGACGCCGACTGCCCAACAAATCCGACAGGTGGCTGCACGGCTTCGAAACCATGGGAGGGTTGAAGGCAAGAATGTATTCTACTGGTTTCAGAACCGCCGAGCCAGGGAGAGGCAGAAGACACGTCGTGAACTCATGTCCATGCATCAGTCTGACTCTAAACAACAACAACAACAAACTTTGACTAACAACACAATTAGCAGTCCCCGGGACAAAGAATCTGCAGGAGTTGACTTTGTTCGTTGTTTTGAAGTTGAGCAGCAGAATAGACGCGCACTACCAAGCTTCACTCTTCTTACCGATCAGGAATCATCTTCAATACTGCAAGAGAGGATGATGAGCATGGTGAAAAGCAATTCCTCCGGGCTACATATGGAACATACCGATTCACCTGGACTAACCTCAAACACAAACGTGTCAGCTACATTAATTTACAGAAAGCTCTTGGACTTTCATCACTATTATGATTACAATTACGGTATGGTATTGACTGCACCGAATATTTACAAAGAAGAAGAAGAAGAAAGCACAGAAACTCAAACTCTTAAGCTCTTTCCACTCGAGAGCGACAATCTTAAAGGTGCTAAGGATACCAAATTACCTATGAGCAGAACCACAAGCACAGACGCTACTTCAAATTTCATGGCAGACCAATATTATTAG

Protein Analysis

330

Amino Acids

37.81

Weight (kDa)

8.99

Isoelectric Point (pI)

62.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 88 - 149 7.2e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 175
AccII CGCG 2 cut(s) 262, 603
AciI CCGC 2 cut(s) 260, 427
AclWI GGATC 3 cut(s) 261, 315, 335
AcsI RAATTY 1 cut(s) 967
AcyI GRCGYC 1 cut(s) 335
AdeI CACNNNGTG 1 cut(s) 458
AfaI GTAC 2 cut(s) 65, 99
AfiI CCNNNNNNNGG 5 cut(s) 175, 361, 388, 436, 441
AflII CTTAAG 1 cut(s) 875
AflIII ACRYGT 2 cut(s) 452, 744
AgsI TTSAA 5 cut(s) 160, 395, 581, 648, 966
AjiI CACGTC 1 cut(s) 455
AjnI CCWGG 2 cut(s) 434, 721
AleI CACNNNNGTG 1 cut(s) 743
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 3 cut(s) 261, 315, 335
Ama87I CYCGRG 2 cut(s) 537, 890
ApeKI GCWGC 8 cut(s) 21, 77, 245, 248, 251, 254, 365, 589
ApoI RAATTY 1 cut(s) 967
AseI ATTAAT 1 cut(s) 758
Asp700I GAANNNNTTC 1 cut(s) 407
AspLEI GCGC 1 cut(s) 605
AsuC2I CCSGG 5 cut(s) 287, 325, 538, 539, 694
AsuHPI GGTGA 2 cut(s) 688, 711
AsuII TTCGAA 1 cut(s) 376
AvaI CYCGRG 2 cut(s) 537, 890
BanII GRGCYC 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 455
BbvCI CCTCAGC 1 cut(s) 87
BbvI GCAGC 8 cut(s) 33, 64, 235, 241, 257, 263, 352, 601
BccI CCATC 4 cut(s) 7, 169, 177, 267
BceAI ACGGC 1 cut(s) 386
BciT130I CCWGG 2 cut(s) 436, 723
BciVI GTATCC 1 cut(s) 913
BcnI CCSGG 5 cut(s) 287, 325, 538, 539, 694
BcoDI GTCTC 1 cut(s) 30
BfmI CTRYAG 3 cut(s) 93, 249, 552
BfrI CTTAAG 1 cut(s) 875
BfuI GTATCC 1 cut(s) 913
BisI GCNGC 8 cut(s) 22, 78, 246, 249, 252, 255, 366, 590
BlpI GCTNAGC 1 cut(s) 116
BlsI GCNGC 8 cut(s) 23, 79, 247, 250, 253, 256, 367, 591
BmcAI AGTACT 2 cut(s) 65, 99
Bme1390I CCNGG 7 cut(s) 287, 325, 436, 538, 539, 694, 723
BmeT110I CYCGRG 2 cut(s) 537, 890
BmgBI CACGTC 1 cut(s) 455
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 7 cut(s) 287, 325, 436, 538, 539, 694, 723
BmsI GCATC 2 cut(s) 310, 484
BpiI GAAGAC 1 cut(s) 455
Bpu10I CCTNAGC 2 cut(s) 87, 915
Bpu1102I GCTNAGC 1 cut(s) 116
Bpu14I TTCGAA 1 cut(s) 376
BpuMI CCSGG 5 cut(s) 287, 325, 538, 539, 694
Bsa29I ATCGAT 1 cut(s) 319
BsaHI GRCGYC 1 cut(s) 335
BsaI GGTCTC 1 cut(s) 30
BsaJI CCNNGG 5 cut(s) 285, 382, 435, 536, 537
Bsc4I CCNNNNNNNGG 5 cut(s) 175, 361, 388, 436, 441
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 2 cut(s) 182, 419
BseBI CCWGG 2 cut(s) 436, 723
BseCI ATCGAT 1 cut(s) 319
BseDI CCNNGG 5 cut(s) 285, 382, 435, 536, 537
BseGI GGATG 1 cut(s) 669
BseLI CCNNNNNNNGG 5 cut(s) 175, 361, 388, 436, 441
BseMII CTCAG 2 cut(s) 51, 101
BseNI ACTGG 2 cut(s) 182, 419
BseXI GCAGC 8 cut(s) 33, 64, 235, 241, 257, 263, 352, 601
BsgI GTGCAG 2 cut(s) 351, 807
Bsh1236I CGCG 2 cut(s) 262, 603
BshVI ATCGAT 1 cut(s) 319
BsiHKCI CYCGRG 2 cut(s) 537, 890
BsiSI CCGG 5 cut(s) 242, 287, 324, 538, 693
BslFI GGGAC 2 cut(s) 519, 554
BslI CCNNNNNNNGG 5 cut(s) 175, 361, 388, 436, 441
BsmAI GTCTC 1 cut(s) 30
BsmFI GGGAC 2 cut(s) 519, 554
Bso31I GGTCTC 1 cut(s) 30
BsoBI CYCGRG 2 cut(s) 537, 890
Bsp119I TTCGAA 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 55
Bsp143I GATC 5 cut(s) 69, 266, 320, 327, 631
Bsp1720I GCTNAGC 1 cut(s) 116
Bsp19I CCATGG 1 cut(s) 382
BspACI CCGC 2 cut(s) 260, 427
BspCNI CTCAG 2 cut(s) 52, 100
BspDI ATCGAT 1 cut(s) 319
BspFNI CGCG 2 cut(s) 262, 603
BspLI GGNNCC 1 cut(s) 180
BspMAI CTGCAG 3 cut(s) 97, 253, 556
BspPI GGATC 3 cut(s) 261, 315, 335
BspQI GCTCTTC 1 cut(s) 303
BspT104I TTCGAA 1 cut(s) 376
BspTI CTTAAG 1 cut(s) 875
BspTNI GGTCTC 1 cut(s) 30
BsrFI RCCGGY 1 cut(s) 241
BsrI ACTGG 2 cut(s) 182, 419
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 5 cut(s) 285, 382, 435, 536, 537
BssMI GATC 5 cut(s) 69, 266, 320, 327, 631
BssNI GRCGYC 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 382
Bst2UI CCWGG 2 cut(s) 436, 723
Bst4CI ACNGT 1 cut(s) 809
Bst6I CTCTTC 2 cut(s) 303, 627
BstACI GRCGYC 1 cut(s) 335
BstAFI CTTAAG 1 cut(s) 875
BstAPI GCANNNNNTGC 1 cut(s) 298
BstBI TTCGAA 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 4 cut(s) 60, 87, 116, 915
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 1 cut(s) 669
BstFNI CGCG 2 cut(s) 262, 603
BstHHI GCGC 1 cut(s) 605
BstKTI GATC 5 cut(s) 72, 269, 323, 330, 634
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 5 cut(s) 69, 266, 320, 327, 631
BstMWI GCNNNNNNNGC 4 cut(s) 210, 251, 254, 298
BstNI CCWGG 2 cut(s) 436, 723
BstSCI CCNGG 7 cut(s) 285, 323, 434, 536, 537, 692, 721
BstSFI CTRYAG 3 cut(s) 93, 249, 552
BstUI CGCG 2 cut(s) 262, 603
BstV1I GCAGC 8 cut(s) 33, 64, 235, 241, 257, 263, 352, 601
BstV2I GAAGAC 1 cut(s) 455
BstX2I RGATCY 1 cut(s) 266
BstYI RGATCY 1 cut(s) 266
Bsu15I ATCGAT 1 cut(s) 319
BsuI GTATCC 1 cut(s) 913
BsuTUI ATCGAT 1 cut(s) 319
BtgI CCRYGG 1 cut(s) 382
BtrI CACGTC 1 cut(s) 455
BtsCI GGATG 1 cut(s) 669
BtsI GCAGTG 2 cut(s) 31, 220
BtsIMutI CAGTG 3 cut(s) 31, 189, 220
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 1 cut(s) 605
Cfr10I RCCGGY 1 cut(s) 241
Cfr9I CCCGGG 1 cut(s) 537
ClaI ATCGAT 1 cut(s) 319
CseI GACGC 3 cut(s) 343, 609, 965
Csp6I GTAC 2 cut(s) 64, 98
CviAII CATG 6 cut(s) 133, 383, 466, 472, 673, 973
CviQI GTAC 2 cut(s) 64, 98
DdeI CTNAG 4 cut(s) 60, 87, 116, 915
DpnI GATC 5 cut(s) 71, 268, 322, 329, 633
DpnII GATC 5 cut(s) 69, 266, 320, 327, 631
DraIII CACNNNGTG 1 cut(s) 458
Eam1104I CTCTTC 2 cut(s) 303, 627
EarI CTCTTC 2 cut(s) 303, 627
Eco130I CCWWGG 1 cut(s) 382
Eco24I GRGCYC 1 cut(s) 55
Eco31I GGTCTC 1 cut(s) 30
Eco88I CYCGRG 2 cut(s) 537, 890
EcoRII CCWGG 2 cut(s) 434, 721
EcoT14I CCWWGG 1 cut(s) 382
EcoT22I ATGCAT 1 cut(s) 477
EcoT38I GRGCYC 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 6 cut(s) 136, 386, 469, 475, 676, 976
FaqI GGGAC 2 cut(s) 519, 554
FatI CATG 6 cut(s) 132, 382, 465, 471, 672, 972
FauNDI CATATG 1 cut(s) 702
Fnu4HI GCNGC 8 cut(s) 22, 78, 246, 249, 252, 255, 366, 590
FokI GGATG 1 cut(s) 676
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 8 cut(s) 22, 78, 246, 249, 252, 255, 366, 590
GlaI GCGC 1 cut(s) 604
GluI GCNGC 8 cut(s) 22, 78, 246, 249, 252, 255, 366, 590
HapII CCGG 5 cut(s) 242, 287, 324, 538, 693
HgaI GACGC 3 cut(s) 343, 609, 965
HhaI GCGC 1 cut(s) 605
Hin1I GRCGYC 1 cut(s) 335
Hin1II CATG 6 cut(s) 136, 386, 469, 475, 676, 976
Hin6I GCGC 1 cut(s) 603
HinP1I GCGC 1 cut(s) 603
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 2 cut(s) 113, 613
HinfI GANTC 5 cut(s) 277, 485, 548, 638, 716
HpaII CCGG 5 cut(s) 242, 287, 324, 538, 693
HphI GGTGA 2 cut(s) 688, 711
Hpy166II GTNNAC 3 cut(s) 6, 461, 562
Hpy188I TCNGA 4 cut(s) 174, 356, 423, 484
Hpy188III TCNNGA 6 cut(s) 270, 305, 331, 458, 635, 892
Hpy8I GTNNAC 3 cut(s) 6, 461, 562
Hpy99I CGWCG 1 cut(s) 459
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 1 cut(s) 809
HpyCH4IV ACGT 2 cut(s) 454, 744
HpyCH4V TGCA 9 cut(s) 80, 95, 251, 301, 368, 475, 554, 655, 824
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 251, 254, 298
HpyF3I CTNAG 4 cut(s) 60, 87, 116, 915
HpySE526I ACGT 2 cut(s) 454, 744
Hsp92I GRCGYC 1 cut(s) 335
Hsp92II CATG 6 cut(s) 136, 386, 469, 475, 676, 976
HspAI GCGC 1 cut(s) 603
Kzo9I GATC 5 cut(s) 69, 266, 320, 327, 631
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 2 cut(s) 262, 289
Lsp1109I GCAGC 8 cut(s) 33, 64, 235, 241, 257, 263, 352, 601
LweI GCATC 2 cut(s) 310, 484
MaeII ACGT 2 cut(s) 454, 744
MaeIII GTNAC 1 cut(s) 150
MalI GATC 5 cut(s) 71, 268, 322, 329, 633
MboI GATC 5 cut(s) 69, 266, 320, 327, 631
MboII GAAGA 9 cut(s) 276, 320, 460, 614, 636, 854, 857, 860, 863
MflI RGATCY 1 cut(s) 266
MhlI GDGCHC 1 cut(s) 55
MluCI AATT 6 cut(s) 525, 685, 759, 802, 926, 967
MlyI GAGTC 2 cut(s) 271, 479
MmeI TCCRAC 3 cut(s) 153, 305, 379
MnlI CCTC 7 cut(s) 96, 180, 381, 435, 654, 700, 742
Mph1103I ATGCAT 1 cut(s) 477
MroXI GAANNNNTTC 1 cut(s) 407
MseI TTAA 3 cut(s) 758, 876, 906
MslI CAYNNNNRTG 3 cut(s) 470, 743, 792
MspA1I CMGCKG 2 cut(s) 248, 254
MspCI CTTAAG 1 cut(s) 875
MspI CCGG 5 cut(s) 242, 287, 324, 538, 693
MspR9I CCNGG 7 cut(s) 287, 325, 436, 538, 539, 694, 723
MvaI CCWGG 2 cut(s) 436, 723
MvnI CGCG 2 cut(s) 262, 603
MwoI GCNNNNNNNGC 4 cut(s) 210, 251, 254, 298
NciI CCSGG 5 cut(s) 287, 325, 538, 539, 694
NcoI CCATGG 1 cut(s) 382
NdeI CATATG 1 cut(s) 702
NdeII GATC 5 cut(s) 69, 266, 320, 327, 631
NlaIII CATG 6 cut(s) 136, 386, 469, 475, 676, 976
NlaIV GGNNCC 1 cut(s) 180
NmeAIII GCCGAG 1 cut(s) 455
NsiI ATGCAT 1 cut(s) 477
NspV TTCGAA 1 cut(s) 376
OliI CACNNNNGTG 1 cut(s) 743
PaeR7I CTCGAG 1 cut(s) 890
PciSI GCTCTTC 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 3 cut(s) 548, 638, 716
PflMI CCANNNNNTGG 1 cut(s) 175
PfoI TCCNGGA 1 cut(s) 323
PkrI GCNGC 8 cut(s) 23, 79, 247, 250, 253, 256, 367, 591
PleI GAGTC 2 cut(s) 271, 479
PpsI GAGTC 2 cut(s) 271, 479
PshBI ATTAAT 1 cut(s) 758
Psp6I CCWGG 2 cut(s) 434, 721
PspGI CCWGG 2 cut(s) 434, 721
PspN4I GGNNCC 1 cut(s) 180
PstI CTGCAG 3 cut(s) 97, 253, 556
PsuI RGATCY 1 cut(s) 266
PvuII CAGCTG 2 cut(s) 248, 254
RsaI GTAC 2 cut(s) 65, 99
RsaNI GTAC 2 cut(s) 64, 98
RseI CAYNNNNRTG 3 cut(s) 470, 743, 792
SapI GCTCTTC 1 cut(s) 303
SaqAI TTAA 3 cut(s) 758, 876, 906
SatI GCNGC 8 cut(s) 22, 78, 246, 249, 252, 255, 366, 590
Sau3AI GATC 5 cut(s) 69, 266, 320, 327, 631
ScaI AGTACT 2 cut(s) 65, 99
SchI GAGTC 2 cut(s) 271, 479
ScrFI CCNGG 7 cut(s) 287, 325, 436, 538, 539, 694, 723
SduI GDGCHC 1 cut(s) 55
SfaNI GCATC 2 cut(s) 310, 484
SfcI CTRYAG 3 cut(s) 93, 249, 552
Sfr274I CTCGAG 1 cut(s) 890
SfuI TTCGAA 1 cut(s) 376
SlaI CTCGAG 1 cut(s) 890
SmaI CCCGGG 1 cut(s) 539
SmiMI CAYNNNNRTG 3 cut(s) 470, 743, 792
SmlI CTYRAG 2 cut(s) 875, 890
SmoI CTYRAG 2 cut(s) 875, 890
Sse9I AATT 6 cut(s) 525, 685, 759, 802, 926, 967
SsiI CCGC 2 cut(s) 260, 427
SspI AATATT 2 cut(s) 832, 986
StyD4I CCNGG 7 cut(s) 285, 323, 434, 536, 537, 692, 721
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 1 cut(s) 809
TaiI ACGT 2 cut(s) 457, 747
TaqI TCGA 5 cut(s) 68, 191, 319, 376, 891
TasI AATT 6 cut(s) 525, 685, 759, 802, 926, 967
TatI WGTACW 2 cut(s) 63, 97
TfiI GAWTC 3 cut(s) 548, 638, 716
Tru1I TTAA 3 cut(s) 758, 876, 906
Tru9I TTAA 3 cut(s) 758, 876, 906
TscAI CASTG 3 cut(s) 31, 189, 220
TseI GCWGC 8 cut(s) 21, 77, 245, 248, 251, 254, 365, 589
TspDTI ATGAA 2 cut(s) 773, 961
TspMI CCCGGG 1 cut(s) 537
TspRI CASTG 3 cut(s) 31, 189, 220
Van91I CCANNNNNTGG 1 cut(s) 175
Vha464I CTTAAG 1 cut(s) 875
VspI ATTAAT 1 cut(s) 758
XapI RAATTY 1 cut(s) 967
XhoI CTCGAG 1 cut(s) 890
XmaI CCCGGG 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 407
ZrmI AGTACT 2 cut(s) 65, 99
Zsp2I ATGCAT 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.