Rorug06G0331300

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004660
Physical Location & Seq
Forward (+)
48436070 .. 48437490
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0331300.1

Sequence Viewer

Length: 1284 bp
ATGGCTCACCCGGTGGAGAGTGATCTTTCTCTGGCGGACACGGCGCGAGTTTCTTTGGGTTCGGCTGGGCTTGCGGCTTTGCAGGCTGACCGGTGGTATCTGGTGGGTCTCCTGCTTGGCCCGAAGAGCAAGTTTCCGGGTTTCATAGGGACTATAGCTACGATATGGCGTATCAAATCTGGATTGACGATCCATGAAGTGGGAGAGCGTTTTGTGTTTCAATTTGAACGTGAGACTGAAAGAAAAATGATCCTACATGGAGGACCTTGGTTTTATAGAAATACGATGGTGGTGCTAGGAGGTTATGACGGTATCGGTCTGGTGCACGAGGTTCCACTGCATCTGGTGGAAGCATGGGTTGCAGTTAAGGGCCTTCCGGTGGGTCTTCGAAATAAGGCTGCGCTGAACTTGGTGGGATCAGCCCTGGGGCGTGTGATCCGTTTCGACCAGAATTCCCTATATCGGAAGGAAGCAGAGCAAAGGATCCGATTGGTTTTTGATGTTCGACGGCGACTTAGGGTTTGGATGCTTTTCGAGTTTTCACCGACGGTGGCGTCTGAACTATCGTTTAACTTTGAAAAGGATGTGATGCCGGCCTGGTTGACTAAGGAGAGAGATGAGATCATGGCTCAGATCCAAGCCACGACTATGGCTGGACTGTCATTGACTGTAGGGGAACGTGGGCCTCGGACTGAGGGAGAAGAGATGAGGGTTCAGAGGGCGATGGTGATTGCGTCGAAATCGGTGGTAGAGGGGCCGGTGTTAGTGGGAACTGATCCACCACATCATACCTTGCTGGCTAGTAACTTTCAGCCTTTTCCTTCGCTGTTCGGGGATTTTAACCTAGCTGAGTTCTTGGCAACTTGGACAAAGAAGGCTGGCCATACTCCTCTGGGTAAAGTGGGGAAGAAATCCCCGATGGAGTTGATCGAGTCCATGGGGATGGATAAGGGTAAGGGTACAGAGACTGAACCTTTAATCCAAGTTGACGATGGGATGCAGCTTGTCTTGCAACACAAAAATGGTCAACTGATTGTCTCGCCAAGGAAGAAGAAATTGGGTAGACCAAAGGGAAGTAAGAACAAACCGAAGTGTGCAAAGGTTCTGGAGAATGAGTTTCAAGGTAGGAAGTCTAAGAAGTGGAAGCTTAGGGACAGTGATCGCCCTGGTCAAGTGCTGGAGGAAAAAGAGATAAGCAGTACCTCTGAATATATGGAGCAGCTGCTGACTGATGGTGGCATTTGTGGGGATGTTGGAGCTTCATCCTCAACTAGTAATGTTTAA

Protein Analysis

427

Amino Acids

47.35

Weight (kDa)

9.35

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 31 - 99 2.2e-10 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 553
AccB7I CCANNNNNTGG 1 cut(s) 199
AccI GTMKAC 1 cut(s) 1063
AccII CGCG 1 cut(s) 46
AciI CCGC 2 cut(s) 35, 74
AclWI GGATC 8 cut(s) 184, 244, 424, 430, 478, 491, 628, 770
AcoI YGGCCR 1 cut(s) 880
AcsI RAATTY 1 cut(s) 451
AcyI GRCGYC 1 cut(s) 554
AdeI CACNNNGTG 1 cut(s) 13
AfaI GTAC 2 cut(s) 961, 1201
AfiI CCNNNNNNNGG 3 cut(s) 199, 379, 462
AgeI ACCGGT 1 cut(s) 90
AgsI TTSAA 4 cut(s) 221, 227, 578, 1121
AhlI ACTAGT 1 cut(s) 1271
AjnI CCWGG 3 cut(s) 423, 596, 1165
AjuI GAANNNNNNNTTGG 2 cut(s) 1040, 1072
AloI GAACNNNNNNTCC 2 cut(s) 963, 995
AluBI AGCT 6 cut(s) 158, 848, 1003, 1147, 1222, 1259
AluI AGCT 6 cut(s) 158, 848, 1003, 1147, 1222, 1259
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 4 cut(s) 113, 227, 959, 1042
Alw44I GTGCAC 1 cut(s) 323
AlwI GGATC 8 cut(s) 184, 244, 424, 430, 478, 491, 628, 770
AlwNI CAGNNNCTG 2 cut(s) 968, 1225
AoxI GGCC 6 cut(s) 118, 370, 594, 683, 755, 880
ApaLI GTGCAC 1 cut(s) 323
ApeKI GCWGC 4 cut(s) 398, 1000, 1219, 1222
ApoI RAATTY 1 cut(s) 451
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AsiGI ACCGGT 1 cut(s) 90
AspLEI GCGC 2 cut(s) 46, 403
AspS9I GGNCC 5 cut(s) 119, 263, 370, 683, 755
AsuC2I CCSGG 2 cut(s) 11, 138
AsuHPI GGTGA 2 cut(s) 534, 739
AsuII TTCGAA 1 cut(s) 388
AvaII GGWCC 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 327
BalI TGGCCA 1 cut(s) 882
BamHI GGATCC 1 cut(s) 483
BauI CACGAG 1 cut(s) 326
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 4 cut(s) 385, 1012, 1209, 1231
BccI CCATC 6 cut(s) 280, 718, 913, 937, 986, 1226
BceAI ACGGC 2 cut(s) 57, 524
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 3 cut(s) 425, 598, 1167
BcnI CCSGG 2 cut(s) 11, 138
BcoDI GTCTC 4 cut(s) 113, 227, 959, 1042
BcuI ACTAGT 1 cut(s) 1271
BfaI CTAG 4 cut(s) 296, 801, 845, 1272
BfmI CTRYAG 2 cut(s) 153, 669
BisI GCNGC 5 cut(s) 75, 399, 1001, 1220, 1223
BlsI GCNGC 5 cut(s) 76, 400, 1002, 1221, 1224
Bme1390I CCNGG 5 cut(s) 11, 138, 425, 598, 1167
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 5 cut(s) 119, 263, 370, 683, 755
BmiI GGNNCC 3 cut(s) 333, 485, 756
BmrFI CCNGG 5 cut(s) 11, 138, 425, 598, 1167
BmsI GCATC 4 cut(s) 349, 516, 579, 987
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 2 cut(s) 1127, 1199
Bpu10I CCTNAGC 1 cut(s) 1148
Bpu14I TTCGAA 1 cut(s) 388
BpuMI CCSGG 2 cut(s) 11, 138
BsaHI GRCGYC 1 cut(s) 554
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 7 cut(s) 266, 423, 424, 686, 936, 1043, 1165
BsaWI WCCGGW 2 cut(s) 90, 376
BsaXI ACNNNNNCTCC 2 cut(s) 1100, 1130
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 379, 462
Bse118I RCCGGY 3 cut(s) 90, 592, 757
BseBI CCWGG 3 cut(s) 425, 598, 1167
BseDI CCNNGG 7 cut(s) 266, 423, 424, 686, 936, 1043, 1165
BseGI GGATG 6 cut(s) 531, 589, 948, 1002, 1255, 1262
BseLI CCNNNNNNNGG 3 cut(s) 199, 379, 462
BseMII CTCAG 3 cut(s) 644, 684, 840
BseRI GAGGAG 1 cut(s) 879
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 4 cut(s) 385, 1012, 1209, 1231
BseYI CCCAGC 1 cut(s) 65
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 6 cut(s) 120, 372, 596, 685, 757, 882
BshTI ACCGGT 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 327
BsiSI CCGG 6 cut(s) 11, 91, 137, 377, 593, 758
BslFI GGGAC 2 cut(s) 163, 1166
BslI CCNNNNNNNGG 3 cut(s) 199, 379, 462
BsmAI GTCTC 4 cut(s) 113, 227, 959, 1042
BsmFI GGGAC 2 cut(s) 163, 1166
BsnI GGCC 6 cut(s) 120, 372, 596, 685, 757, 882
Bso31I GGTCTC 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 327
Bsp19I CCATGG 1 cut(s) 936
BspACI CCGC 2 cut(s) 35, 74
BspANI GGCC 6 cut(s) 120, 372, 596, 685, 757, 882
BspCNI CTCAG 3 cut(s) 643, 685, 841
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 333, 485, 756
BspPI GGATC 8 cut(s) 184, 244, 424, 430, 478, 491, 628, 770
BspQI GCTCTTC 1 cut(s) 119
BspT104I TTCGAA 1 cut(s) 388
BspTNI GGTCTC 1 cut(s) 113
BsrFI RCCGGY 3 cut(s) 90, 592, 757
BssAI RCCGGY 3 cut(s) 90, 592, 757
BssECI CCNNGG 7 cut(s) 266, 423, 424, 686, 936, 1043, 1165
BssNI GRCGYC 1 cut(s) 554
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 3 cut(s) 266, 936, 1043
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 3 cut(s) 425, 598, 1167
Bst4CI ACNGT 5 cut(s) 311, 550, 660, 670, 1157
Bst6I CTCTTC 2 cut(s) 119, 696
BstACI GRCGYC 1 cut(s) 554
BstAPI GCANNNNNTGC 1 cut(s) 359
BstBI TTCGAA 1 cut(s) 388
BstC8I GCNNGC 5 cut(s) 72, 84, 594, 798, 880
BstDEI CTNAG 7 cut(s) 515, 606, 630, 693, 849, 1134, 1148
BstDSI CCRYGG 1 cut(s) 936
BstF5I GGATG 6 cut(s) 531, 589, 948, 1002, 1255, 1262
BstFNI CGCG 1 cut(s) 46
BstHHI GCGC 2 cut(s) 46, 403
BstMAI GTCTC 4 cut(s) 113, 227, 959, 1042
BstMWI GCNNNNNNNGC 6 cut(s) 41, 71, 83, 126, 359, 1009
BstNI CCWGG 3 cut(s) 425, 598, 1167
BstSCI CCNGG 5 cut(s) 9, 136, 423, 596, 1165
BstSFI CTRYAG 2 cut(s) 153, 669
BstSLI GKGCMC 1 cut(s) 327
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 4 cut(s) 385, 1012, 1209, 1231
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 2 cut(s) 483, 633
BstXI CCANNNNNNTGG 2 cut(s) 649, 943
BstYI RGATCY 2 cut(s) 483, 633
BsuRI GGCC 6 cut(s) 120, 372, 596, 685, 757, 882
BtgI CCRYGG 1 cut(s) 936
BtgZI GCGATG 1 cut(s) 737
BtsCI GGATG 6 cut(s) 531, 589, 948, 1002, 1255, 1262
BtsI GCAGTG 1 cut(s) 335
BtsIMutI CAGTG 2 cut(s) 335, 1162
Cac8I GCNNGC 5 cut(s) 72, 84, 594, 798, 880
CaiI CAGNNNCTG 2 cut(s) 968, 1225
CfoI GCGC 2 cut(s) 46, 403
Cfr10I RCCGGY 3 cut(s) 90, 592, 757
Cfr13I GGNCC 5 cut(s) 119, 263, 370, 683, 755
CseI GACGC 2 cut(s) 543, 723
Csp6I GTAC 2 cut(s) 960, 1200
CspAI ACCGGT 1 cut(s) 90
CviAII CATG 5 cut(s) 194, 257, 354, 625, 937
CviQI GTAC 2 cut(s) 960, 1200
DdeI CTNAG 7 cut(s) 515, 606, 630, 693, 849, 1134, 1148
DraIII CACNNNGTG 1 cut(s) 13
DrdI GACNNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 553
EaeI YGGCCR 1 cut(s) 880
Eam1104I CTCTTC 2 cut(s) 119, 696
EarI CTCTTC 2 cut(s) 119, 696
EciI GGCGGA 1 cut(s) 50
Eco130I CCWWGG 3 cut(s) 266, 936, 1043
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 1 cut(s) 263
EcoO109I RGGNCCY 2 cut(s) 263, 370
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 3 cut(s) 423, 596, 1165
EcoT14I CCWWGG 3 cut(s) 266, 936, 1043
ErhI CCWWGG 3 cut(s) 266, 936, 1043
FaeI CATG 5 cut(s) 197, 260, 357, 628, 940
FaqI GGGAC 2 cut(s) 163, 1166
FatI CATG 5 cut(s) 193, 256, 353, 624, 936
FblI GTMKAC 1 cut(s) 1063
Fnu4HI GCNGC 5 cut(s) 75, 399, 1001, 1220, 1223
FokI GGATG 6 cut(s) 538, 596, 955, 1009, 1249, 1262
Fsp4HI GCNGC 5 cut(s) 75, 399, 1001, 1220, 1223
FspBI CTAG 4 cut(s) 296, 801, 845, 1272
GlaI GCGC 2 cut(s) 45, 402
GluI GCNGC 5 cut(s) 75, 399, 1001, 1220, 1223
GsaI CCCAGC 1 cut(s) 69
GsuI CTGGAG 2 cut(s) 1127, 1199
HaeIII GGCC 6 cut(s) 120, 372, 596, 685, 757, 882
HapII CCGG 6 cut(s) 11, 91, 137, 377, 593, 758
HgaI GACGC 2 cut(s) 543, 723
HhaI GCGC 2 cut(s) 46, 403
Hin1I GRCGYC 1 cut(s) 554
Hin1II CATG 5 cut(s) 197, 260, 357, 628, 940
Hin6I GCGC 2 cut(s) 44, 401
HinP1I GCGC 2 cut(s) 44, 401
HincII GTYRAC 3 cut(s) 603, 988, 1028
HindII GTYRAC 3 cut(s) 603, 988, 1028
HindIII AAGCTT 1 cut(s) 1145
HinfI GANTC 1 cut(s) 932
HpaII CCGG 6 cut(s) 11, 91, 137, 377, 593, 758
HphI GGTGA 2 cut(s) 534, 739
Hpy166II GTNNAC 5 cut(s) 325, 603, 988, 1028, 1064
Hpy188I TCNGA 7 cut(s) 465, 488, 559, 633, 690, 717, 1207
Hpy188III TCNNGA 2 cut(s) 180, 1106
Hpy8I GTNNAC 5 cut(s) 325, 603, 988, 1028, 1064
Hpy99I CGWCG 3 cut(s) 510, 550, 739
HpyAV CCTTC 4 cut(s) 383, 460, 831, 868
HpyCH4III ACNGT 5 cut(s) 311, 550, 660, 670, 1157
HpyCH4IV ACGT 2 cut(s) 229, 679
HpyCH4V TGCA 7 cut(s) 82, 325, 340, 362, 1000, 1012, 1097
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 71, 83, 126, 359, 1009
HpyF3I CTNAG 7 cut(s) 515, 606, 630, 693, 849, 1134, 1148
HpySE526I ACGT 2 cut(s) 229, 679
Hsp92I GRCGYC 1 cut(s) 554
Hsp92II CATG 5 cut(s) 197, 260, 357, 628, 940
HspAI GCGC 2 cut(s) 44, 401
KroI GCCGGC 1 cut(s) 592
KroNI GCCGGC 1 cut(s) 594
LguI GCTCTTC 1 cut(s) 119
LmnI GCTCC 2 cut(s) 1216, 1256
Lsp1109I GCAGC 4 cut(s) 385, 1012, 1209, 1231
LweI GCATC 4 cut(s) 349, 516, 579, 987
MaeI CTAG 4 cut(s) 296, 801, 845, 1272
MaeII ACGT 2 cut(s) 229, 679
MaeIII GTNAC 1 cut(s) 803
MboII GAAGA 6 cut(s) 136, 377, 713, 919, 1060, 1063
MflI RGATCY 2 cut(s) 483, 633
MhlI GDGCHC 1 cut(s) 327
MlsI TGGCCA 1 cut(s) 882
MluCI AATT 3 cut(s) 221, 451, 1055
MluNI TGGCCA 1 cut(s) 882
MlyI GAGTC 1 cut(s) 941
MmeI TCCRAC 1 cut(s) 1234
Mox20I TGGCCA 1 cut(s) 882
MroNI GCCGGC 1 cut(s) 592
MscI TGGCCA 1 cut(s) 882
MseI TTAA 5 cut(s) 366, 570, 840, 977, 1282
MslI CAYNNNNRTG 3 cut(s) 647, 941, 1020
Msp20I TGGCCA 1 cut(s) 882
MspA1I CMGCKG 1 cut(s) 1222
MspI CCGG 6 cut(s) 11, 91, 137, 377, 593, 758
MspR9I CCNGG 5 cut(s) 11, 138, 425, 598, 1167
MvaI CCWGG 3 cut(s) 425, 598, 1167
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 6 cut(s) 41, 71, 83, 126, 359, 1009
NaeI GCCGGC 1 cut(s) 594
NciI CCSGG 2 cut(s) 11, 138
NcoI CCATGG 1 cut(s) 936
NgoMIV GCCGGC 1 cut(s) 592
NlaIII CATG 5 cut(s) 197, 260, 357, 628, 940
NlaIV GGNNCC 3 cut(s) 333, 485, 756
NspV TTCGAA 1 cut(s) 388
PasI CCCWGGG 1 cut(s) 424
PciSI GCTCTTC 1 cut(s) 119
PdiI GCCGGC 1 cut(s) 594
PflMI CCANNNNNTGG 1 cut(s) 199
PinAI ACCGGT 1 cut(s) 90
PkrI GCNGC 5 cut(s) 76, 400, 1002, 1221, 1224
PleI GAGTC 1 cut(s) 940
PpsI GAGTC 1 cut(s) 940
PpuMI RGGWCCY 1 cut(s) 263
Psp5II RGGWCCY 1 cut(s) 263
Psp6I CCWGG 3 cut(s) 423, 596, 1165
PspFI CCCAGC 1 cut(s) 65
PspGI CCWGG 3 cut(s) 423, 596, 1165
PspN4I GGNNCC 3 cut(s) 333, 485, 756
PspPI GGNCC 5 cut(s) 119, 263, 370, 683, 755
PspPPI RGGWCCY 1 cut(s) 263
PstNI CAGNNNCTG 2 cut(s) 968, 1225
PsuI RGATCY 2 cut(s) 483, 633
PvuII CAGCTG 1 cut(s) 1222
RsaI GTAC 2 cut(s) 961, 1201
RsaNI GTAC 2 cut(s) 960, 1200
RseI CAYNNNNRTG 3 cut(s) 647, 941, 1020
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 5 cut(s) 366, 570, 840, 977, 1282
SatI GCNGC 5 cut(s) 75, 399, 1001, 1220, 1223
Sau96I GGNCC 5 cut(s) 119, 263, 370, 683, 755
SchI GAGTC 1 cut(s) 941
ScrFI CCNGG 5 cut(s) 11, 138, 425, 598, 1167
SduI GDGCHC 1 cut(s) 327
SfaNI GCATC 4 cut(s) 349, 516, 579, 987
SfcI CTRYAG 2 cut(s) 153, 669
SfuI TTCGAA 1 cut(s) 388
SinI GGWCC 1 cut(s) 263
SmiMI CAYNNNNRTG 3 cut(s) 647, 941, 1020
SpeI ACTAGT 1 cut(s) 1271
Sse9I AATT 3 cut(s) 221, 451, 1055
SsiI CCGC 2 cut(s) 35, 74
SspMI CTAG 4 cut(s) 296, 801, 845, 1272
StyD4I CCNGG 5 cut(s) 9, 136, 423, 596, 1165
StyI CCWWGG 3 cut(s) 266, 936, 1043
TaaI ACNGT 5 cut(s) 311, 550, 660, 670, 1157
TaiI ACGT 2 cut(s) 232, 682
TaqI TCGA 6 cut(s) 388, 444, 505, 534, 737, 930
TaqII GACCGA 1 cut(s) 305
TasI AATT 3 cut(s) 221, 451, 1055
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 5 cut(s) 366, 570, 840, 977, 1282
Tru9I TTAA 5 cut(s) 366, 570, 840, 977, 1282
TscAI CASTG 2 cut(s) 342, 1162
TseI GCWGC 4 cut(s) 398, 1000, 1219, 1222
TspDTI ATGAA 3 cut(s) 133, 210, 1251
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 2 cut(s) 342, 1162
Van91I CCANNNNNTGG 1 cut(s) 199
VneI GTGCAC 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 989
XmiI GTMKAC 1 cut(s) 1063
XspI CTAG 4 cut(s) 296, 801, 845, 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.