Rorug06G0477900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007875
Physical Location & Seq
Reverse (-)
59951290 .. 59956708
5419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0477900.1

Sequence Viewer

Length: 993 bp
ATGACAACAAGAACAGACCTTCAGGGTGTACAGATTTGGGGCTTTTTGTCTTACATATCATCAGTAACCATGGGATCTACCAAAGGTGCGGAACTCCTCACGAACAAGGAGAAGGTCATTTTCATCATGGGATCGACTGGATCCGGAAAAACCAAACTCTCTGTAGATTTAGCCCTACAGTTTAAGGGTGAAATCATCAATTCTGACCAAATTCAAGTCTACAAAGGTGGCGATATTATTACTAATAAAGTCTCGGAGATCGAGCGCCAAGGAATCCGCCACCATTTGCTAGGGTTTATTGAAGACACTGAGGAGGACTTCACCATAGAAGATTTTTGCCACCATGTACAAGGTACCATTAAAGAAATTATTAACCGCAATTCGATCCCAATCATTGCAGGTGGATCAAACTCGTACATCGAGGCACTCGTTGAAGATCCAAAAATTAATTTTCGAGCTAAATATGATTGTTGCTTCCTATGGCTCGATGCTTCCTTACCAGTAATATATGCTCATGTGTCAAGAAGAGTTGATCAGATGGTGGAGGCAGGTGTTGTGAATGAGCTAAGACGATTTTGTGTTCCCGGGGCTGATTATGAACGTGGCATCCGACGAGCGATTGGAGCTCAGGAGATGGATCCCTTTTTCATGGCGGAGAGAAATAATGAAGATGAGGATACCAAGATGTCATTGCTTGAAGCTGGCATCAAAGATATTAAGGAAAACACCTGCAAGCTCGTTGATCGCCAAACAGAAAAGATTCGACGTCTGAAGGATGAACTTGGATGGGACATTCATCGACTTGATGTAACATGTGTGCATCAGAGACGTGGAGAAGAAGCTGAGAATGCATGGAGAGCTTTTGTTTTGGAACCGAGTTTGGCTATTGTGGAATGTTTTTTGAAGAATTTTCCTTATCCAGGACAGTCAAAACCTACGTGGTCTATGTTCGGGAAAATTTTTAACAAAATTGCAGCGTTGAGAAATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

330

Amino Acids

37.51

Weight (kDa)

5.76

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IPT PF01745 38 - 183 6.5e-07 Isopentenyl transferase
IPPT PF01715 72 - 146 5.9e-20 IPP transferase
IPPT PF01715 152 - 255 1.9e-11 IPP transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 389, 539, 737
AatII GACGTC 1 cut(s) 769
Acc36I ACCTGC 3 cut(s) 389, 539, 737
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 353
AccI GTMKAC 1 cut(s) 219
AccIII TCCGGA 1 cut(s) 143
AciI CCGC 4 cut(s) 89, 277, 376, 653
AclWI GGATC 9 cut(s) 82, 135, 139, 148, 379, 412, 431, 632, 645
AcsI RAATTY 3 cut(s) 210, 907, 957
AcuI CTGAAG 2 cut(s) 5, 791
AcyI GRCGYC 1 cut(s) 766
AfaI GTAC 4 cut(s) 30, 348, 355, 416
AfiI CCNNNNNNNGG 1 cut(s) 920
AflIII ACRYGT 1 cut(s) 812
AgsI TTSAA 5 cut(s) 215, 302, 434, 698, 904
AjiI CACGTC 1 cut(s) 830
AjnI CCWGG 1 cut(s) 919
AloI GAACNNNNNNTCC 2 cut(s) 591, 623
AluBI AGCT 7 cut(s) 458, 565, 626, 701, 736, 842, 860
AluI AGCT 7 cut(s) 458, 565, 626, 701, 736, 842, 860
Alw21I GWGCWC 1 cut(s) 628
Alw26I GTCTC 2 cut(s) 256, 820
AlwI GGATC 9 cut(s) 82, 135, 139, 148, 379, 412, 431, 632, 645
Ama87I CYCGRG 1 cut(s) 584
Aor13HI TCCGGA 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 974
ApoI RAATTY 3 cut(s) 210, 907, 957
AseI ATTAAT 1 cut(s) 447
Asp700I GAANNNNTTC 1 cut(s) 759
Asp718I GGTACC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 267
AsuC2I CCSGG 2 cut(s) 585, 586
AsuHPI GGTGA 2 cut(s) 200, 313
AvaI CYCGRG 1 cut(s) 584
BamHI GGATCC 2 cut(s) 140, 637
BanI GGYRCC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 628
BbsI GAAGAC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 1 cut(s) 986
BccI CCATC 3 cut(s) 532, 628, 780
BciT130I CCWGG 1 cut(s) 921
BciVI GTATCC 1 cut(s) 670
BclI TGATCA 1 cut(s) 532
BcnI CCSGG 2 cut(s) 585, 586
BcoDI GTCTC 2 cut(s) 256, 820
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 2 cut(s) 162, 176
BfoI RGCGCY 1 cut(s) 268
BfuAI ACCTGC 3 cut(s) 389, 539, 737
BfuI GTATCC 1 cut(s) 670
BisI GCNGC 1 cut(s) 975
BlsI GCNGC 1 cut(s) 976
Bme1390I CCNGG 3 cut(s) 585, 586, 921
BmeT110I CYCGRG 1 cut(s) 584
BmgBI CACGTC 1 cut(s) 830
BmiI GGNNCC 4 cut(s) 142, 355, 639, 873
BmrFI CCNGG 3 cut(s) 585, 586, 921
BmsI GCATC 4 cut(s) 478, 615, 714, 829
BpiI GAAGAC 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 627
BpuMI CCSGG 2 cut(s) 585, 586
BsaAI YACGTR 1 cut(s) 939
BsaBI GATNNNNATC 1 cut(s) 389
BsaHI GRCGYC 1 cut(s) 766
BsaJI CCNNGG 4 cut(s) 69, 268, 584, 585
BsaWI WCCGGW 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 920
Bse1I ACTGG 2 cut(s) 142, 500
Bse3DI GCAATG 2 cut(s) 393, 689
Bse8I GATNNNNATC 1 cut(s) 389
BseAI TCCGGA 1 cut(s) 143
BseBI CCWGG 1 cut(s) 921
BseDI CCNNGG 4 cut(s) 69, 268, 584, 585
BseGI GGATG 3 cut(s) 606, 781, 791
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 1 cut(s) 920
BseMI GCAATG 2 cut(s) 393, 689
BseMII CTCAG 3 cut(s) 300, 641, 834
BseNI ACTGG 2 cut(s) 142, 500
BseRI GAGGAG 2 cut(s) 86, 326
BseXI GCAGC 1 cut(s) 986
BshNI GGYRCC 1 cut(s) 353
BsiHKAI GWGCWC 1 cut(s) 628
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 2 cut(s) 144, 585
BslFI GGGAC 1 cut(s) 803
BslI CCNNNNNNNGG 1 cut(s) 920
BsmAI GTCTC 2 cut(s) 256, 820
BsmBI CGTCTC 1 cut(s) 820
BsmFI GGGAC 1 cut(s) 803
BsmI GAATGC 1 cut(s) 853
BsoBI CYCGRG 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 628
Bsp13I TCCGGA 1 cut(s) 143
Bsp1407I TGTACA 2 cut(s) 28, 346
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 4 cut(s) 89, 277, 376, 653
BspCNI CTCAG 3 cut(s) 301, 640, 835
BspEI TCCGGA 1 cut(s) 143
BspLI GGNNCC 4 cut(s) 142, 355, 639, 873
BspMI ACCTGC 3 cut(s) 389, 539, 737
BspPI GGATC 9 cut(s) 82, 135, 139, 148, 379, 412, 431, 632, 645
BspT107I GGYRCC 1 cut(s) 353
BsrDI GCAATG 2 cut(s) 393, 689
BsrGI TGTACA 2 cut(s) 28, 346
BsrI ACTGG 2 cut(s) 142, 500
BssECI CCNNGG 4 cut(s) 69, 268, 584, 585
BssNI GRCGYC 1 cut(s) 766
BssT1I CCWWGG 2 cut(s) 69, 268
Bst2UI CCWGG 1 cut(s) 921
Bst4CI ACNGT 2 cut(s) 180, 927
Bst6I CTCTTC 1 cut(s) 520
BstACI GRCGYC 1 cut(s) 766
BstAUI TGTACA 2 cut(s) 28, 346
BstBAI YACGTR 1 cut(s) 939
BstC8I GCNNGC 2 cut(s) 703, 734
BstDEI CTNAG 4 cut(s) 309, 566, 627, 843
BstDSI CCRYGG 1 cut(s) 69
BstENI CCTNNNNNAGG 1 cut(s) 918
BstF5I GGATG 3 cut(s) 606, 781, 791
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 1 cut(s) 267
BstMAI GTCTC 2 cut(s) 256, 820
BstMWI GCNNNNNNNGC 3 cut(s) 623, 848, 857
BstNI CCWGG 1 cut(s) 921
BstNSI RCATGY 1 cut(s) 816
BstSCI CCNGG 3 cut(s) 583, 584, 919
BstSFI CTRYAG 2 cut(s) 162, 176
BstV1I GCAGC 1 cut(s) 986
BstV2I GAAGAC 1 cut(s) 309
BstX2I RGATCY 4 cut(s) 74, 140, 436, 637
BstYI RGATCY 4 cut(s) 74, 140, 436, 637
BsuI GTATCC 1 cut(s) 670
BtgI CCRYGG 1 cut(s) 69
BtrI CACGTC 1 cut(s) 830
BtsCI GGATG 3 cut(s) 606, 781, 791
BtsIMutI CAGTG 1 cut(s) 306
BveI ACCTGC 3 cut(s) 389, 539, 737
Cac8I GCNNGC 2 cut(s) 703, 734
CfoI GCGC 1 cut(s) 267
Cfr9I CCCGGG 1 cut(s) 584
Csp6I GTAC 4 cut(s) 29, 347, 354, 415
CviAII CATG 7 cut(s) 70, 127, 344, 515, 649, 813, 852
CviQI GTAC 4 cut(s) 29, 347, 354, 415
DdeI CTNAG 4 cut(s) 309, 566, 627, 843
Eam1104I CTCTTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 520
EciI GGCGGA 2 cut(s) 266, 668
Ecl136II GAGCTC 1 cut(s) 626
Eco130I CCWWGG 2 cut(s) 69, 268
Eco24I GRGCYC 1 cut(s) 628
Eco53kI GAGCTC 1 cut(s) 626
Eco57I CTGAAG 2 cut(s) 5, 791
Eco88I CYCGRG 1 cut(s) 584
EcoICRI GAGCTC 1 cut(s) 626
EcoNI CCTNNNNNAGG 1 cut(s) 918
EcoRII CCWGG 1 cut(s) 919
EcoT14I CCWWGG 2 cut(s) 69, 268
EcoT22I ATGCAT 1 cut(s) 853
EcoT38I GRGCYC 1 cut(s) 628
ErhI CCWWGG 2 cut(s) 69, 268
Esp3I CGTCTC 1 cut(s) 820
FaeI CATG 7 cut(s) 73, 130, 347, 518, 652, 816, 855
FaqI GGGAC 1 cut(s) 803
FatI CATG 7 cut(s) 69, 126, 343, 514, 648, 812, 851
FbaI TGATCA 1 cut(s) 532
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 1 cut(s) 975
FokI GGATG 3 cut(s) 593, 788, 798
FriOI GRGCYC 1 cut(s) 628
Fsp4HI GCNGC 1 cut(s) 975
FspBI CTAG 1 cut(s) 290
GlaI GCGC 1 cut(s) 266
GluI GCNGC 1 cut(s) 975
HaeII RGCGCY 1 cut(s) 268
HapII CCGG 2 cut(s) 144, 585
HhaI GCGC 1 cut(s) 267
Hin1I GRCGYC 1 cut(s) 766
Hin1II CATG 7 cut(s) 73, 130, 347, 518, 652, 816, 855
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HinfI GANTC 2 cut(s) 273, 760
HpaII CCGG 2 cut(s) 144, 585
HphI GGTGA 2 cut(s) 200, 313
Hpy166II GTNNAC 2 cut(s) 29, 220
Hpy188I TCNGA 7 cut(s) 205, 256, 537, 611, 771, 825, 992
Hpy188III TCNNGA 5 cut(s) 100, 144, 522, 629, 952
Hpy8I GTNNAC 2 cut(s) 29, 220
Hpy99I CGWCG 2 cut(s) 615, 768
HpyAV CCTTC 3 cut(s) 29, 106, 766
HpyCH4III ACNGT 2 cut(s) 180, 927
HpyCH4IV ACGT 4 cut(s) 601, 766, 829, 938
HpyCH4V TGCA 5 cut(s) 398, 732, 820, 851, 974
HpyF10VI GCNNNNNNNGC 3 cut(s) 623, 848, 857
HpyF3I CTNAG 4 cut(s) 309, 566, 627, 843
HpySE526I ACGT 4 cut(s) 601, 766, 829, 938
Hsp92I GRCGYC 1 cut(s) 766
Hsp92II CATG 7 cut(s) 73, 130, 347, 518, 652, 816, 855
HspAI GCGC 1 cut(s) 265
Kpn2I TCCGGA 1 cut(s) 143
KpnI GGTACC 1 cut(s) 357
Ksp22I TGATCA 1 cut(s) 532
LmnI GCTCC 1 cut(s) 623
Lsp1109I GCAGC 1 cut(s) 986
LweI GCATC 4 cut(s) 478, 615, 714, 829
MaeI CTAG 1 cut(s) 290
MaeII ACGT 4 cut(s) 601, 766, 829, 938
MaeIII GTNAC 2 cut(s) 64, 808
MboII GAAGA 7 cut(s) 314, 341, 446, 537, 680, 848, 916
MflI RGATCY 4 cut(s) 74, 140, 436, 637
MhlI GDGCHC 1 cut(s) 628
MluCI AATT 9 cut(s) 199, 210, 366, 379, 444, 448, 907, 957, 969
MmeI TCCRAC 1 cut(s) 634
MnlI CCTC 6 cut(s) 107, 304, 307, 415, 538, 667
Mph1103I ATGCAT 1 cut(s) 853
MroI TCCGGA 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 759
MseI TTAA 6 cut(s) 183, 360, 372, 447, 717, 963
MspI CCGG 2 cut(s) 144, 585
MspR9I CCNGG 3 cut(s) 585, 586, 921
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 1 cut(s) 921
MwoI GCNNNNNNNGC 3 cut(s) 623, 848, 857
NciI CCSGG 2 cut(s) 585, 586
NcoI CCATGG 1 cut(s) 69
NlaIII CATG 7 cut(s) 73, 130, 347, 518, 652, 816, 855
NlaIV GGNNCC 4 cut(s) 142, 355, 639, 873
NsiI ATGCAT 1 cut(s) 853
NspI RCATGY 1 cut(s) 816
PaqCI CACCTGC 3 cut(s) 389, 539, 737
PciI ACATGT 1 cut(s) 812
PcsI WCGNNNNNNNCGW 2 cut(s) 426, 607
PctI GAATGC 1 cut(s) 853
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 2 cut(s) 273, 760
PfoI TCCNGGA 1 cut(s) 919
PkrI GCNGC 1 cut(s) 976
Ppu21I YACGTR 1 cut(s) 939
PscI ACATGT 1 cut(s) 812
PshBI ATTAAT 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 628
Psp6I CCWGG 1 cut(s) 919
PspGI CCWGG 1 cut(s) 919
PspN4I GGNNCC 4 cut(s) 142, 355, 639, 873
PsuI RGATCY 4 cut(s) 74, 140, 436, 637
RsaI GTAC 4 cut(s) 30, 348, 355, 416
RsaNI GTAC 4 cut(s) 29, 347, 354, 415
SacI GAGCTC 1 cut(s) 628
SaqAI TTAA 6 cut(s) 183, 360, 372, 447, 717, 963
SatI GCNGC 1 cut(s) 975
ScrFI CCNGG 3 cut(s) 585, 586, 921
SduI GDGCHC 1 cut(s) 628
SfaNI GCATC 4 cut(s) 478, 615, 714, 829
SfcI CTRYAG 2 cut(s) 162, 176
SmaI CCCGGG 1 cut(s) 586
Sse9I AATT 9 cut(s) 199, 210, 366, 379, 444, 448, 907, 957, 969
SsiI CCGC 4 cut(s) 89, 277, 376, 653
SspMI CTAG 1 cut(s) 290
SstI GAGCTC 1 cut(s) 628
StyD4I CCNGG 3 cut(s) 583, 584, 919
StyI CCWWGG 2 cut(s) 69, 268
TaaI ACNGT 2 cut(s) 180, 927
TaiI ACGT 4 cut(s) 604, 769, 832, 941
TaqI TCGA 8 cut(s) 134, 261, 383, 420, 454, 486, 763, 799
TasI AATT 9 cut(s) 199, 210, 366, 379, 444, 448, 907, 957, 969
TatI WGTACW 2 cut(s) 28, 346
TfiI GAWTC 2 cut(s) 273, 760
Tru1I TTAA 6 cut(s) 183, 360, 372, 447, 717, 963
Tru9I TTAA 6 cut(s) 183, 360, 372, 447, 717, 963
TscAI CASTG 1 cut(s) 313
TseI GCWGC 1 cut(s) 974
TspDTI ATGAA 6 cut(s) 112, 612, 637, 681, 785, 792
TspMI CCCGGG 1 cut(s) 584
TspRI CASTG 1 cut(s) 313
VspI ATTAAT 1 cut(s) 447
XagI CCTNNNNNAGG 1 cut(s) 918
XapI RAATTY 3 cut(s) 210, 907, 957
XceI RCATGY 1 cut(s) 816
XmaI CCCGGG 1 cut(s) 584
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 1 cut(s) 290
ZraI GACGTC 1 cut(s) 767
Zsp2I ATGCAT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.