Rorug06G0502100

Protein transport protein Sec61 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003532
Physical Location & Seq
Forward (+)
61666579 .. 61670547
3969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0502100.1

Sequence Viewer

Length: 1161 bp
ATGAAAGCAGCACTATCCACTCTGATCAGCTCCAGGACTCTGAGCCCTCCCCCCTCTCCTTCTTCTGCTTCTTCTTCTTCTTTCGCTCGCTCCATTTGGCTTCAACCAAAGACCCTCTCCAACTCACCCTCCTTCATTACCCCTATACCCTCCAAGACCCTCACCACCAACAATTACTCCCGTCTCCGTCTCCGTCTCAATCATCGCAAGTCCCTCACCGCTAATTTCTCGTCGATAATGGCCTCCGCTTACAAGCCCGAAGAAGCCAGGGTCCCACCTGCCCTTCCCTTGCCCACTCCTCCCGTCAACAAGTTTAAGATTGGACTGTGCCAATTGTCGGTGACGGCCGATAAGGAGAGGAACATTGCTCATGCTCGCAAGGCCATCGAGCAGGCTGTCGAAAAGGGTGCTCAGCTGGTTCTCCTACCTGAAATATGGAATGGCCCGTATTCAAATGATAGCTTCCCAGTGTTTGCTGAGGATATTGACGCTGGTGGTGAAGCATCTCCTTCGACAGCCATGCTTTCTGAAGTTGCTCAGCGCCTGAAAATCACCATTGTTGGTGGATCTATATCAGAGCGTTCTGGTGATCAGCTGTACAATACTTGTTGTGTCTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACACCTCTTTGATATTGATATCCCTGGGAAGATTACCTTTATTGAATCAAAGACTCTTACAGCAGGGCAAACTCCTACCATTGTGGACACAGAAGTTGGGCGTATTGGCATAGGCATTTGTTATGACATTAGGTTCCAGGAACTAGCTATGATCTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACCACTGGGCCATTGCATTGGGAATTGTTGCAGAGAGCAAGGGCTACAGATAACCAGCTATATGTGGCAACTTGTTCACCTGCTCGAGATGCCGGAGCTGGTTATGTGGCATGGGGCCACTCCACCCTTGTCGGACCATTTGGAGAAGTGCTGGCAACTACAGAACATGAAGAGGCAATTATCATAGCTGAGGTTGATTATTCACTCCTTGAGCTTAGAAGAACAAATCTCCCTTTACTGAAGCAGCGGCGAGGAGATCTTTACCAGTTGGTAGATGTTCAGAGGTTGAATTCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
SecE

Protein Analysis

386

Amino Acids

42.16

Weight (kDa)

9.03

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 106 - 358 9.3e-65 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 286, 949
Acc36I ACCTGC 2 cut(s) 286, 949
AciI CCGC 3 cut(s) 219, 246, 1108
AclWI GGATC 1 cut(s) 574
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 1150
AcuI CTGAAG 2 cut(s) 549, 1121
AfaI GTAC 2 cut(s) 599, 622
AfiI CCNNNNNNNGG 1 cut(s) 337
AgsI TTSAA 4 cut(s) 104, 453, 701, 1150
AjnI CCWGG 5 cut(s) 32, 266, 679, 792, 844
Alw21I GWGCWC 2 cut(s) 412, 829
Alw26I GTCTC 3 cut(s) 188, 194, 200
AlwI GGATC 1 cut(s) 574
AlwNI CAGNNNCTG 1 cut(s) 544
Ama87I CYCGRG 1 cut(s) 945
AoxI GGCC 6 cut(s) 240, 345, 381, 442, 869, 976
ApeKI GCWGC 2 cut(s) 8, 1105
ApoI RAATTY 1 cut(s) 1150
AspLEI GCGC 1 cut(s) 543
AspS9I GGNCC 5 cut(s) 271, 443, 869, 976, 995
AsuHPI GGTGA 8 cut(s) 117, 154, 208, 352, 509, 544, 599, 930
AvaI CYCGRG 1 cut(s) 945
AvaII GGWCC 2 cut(s) 271, 995
BanII GRGCYC 1 cut(s) 47
Bbv12I GWGCWC 2 cut(s) 412, 829
BbvCI CCTCAGC 2 cut(s) 477, 1050
BbvI GCAGC 2 cut(s) 20, 1117
BccI CCATC 2 cut(s) 392, 620
BceAI ACGGC 1 cut(s) 360
BcgI CGANNNNNNTGC 8 cut(s) 367, 389, 401, 423, 492, 502, 526, 536
BciT130I CCWGG 5 cut(s) 34, 268, 681, 794, 846
BclI TGATCA 2 cut(s) 24, 589
BcoDI GTCTC 3 cut(s) 188, 194, 200
BfaI CTAG 1 cut(s) 800
BfmI CTRYAG 2 cut(s) 906, 1020
BfoI RGCGCY 1 cut(s) 544
BfuAI ACCTGC 2 cut(s) 286, 949
BglII AGATCT 1 cut(s) 1117
BisI GCNGC 3 cut(s) 9, 1106, 1109
BlpI GCTNAGC 2 cut(s) 411, 537
BlsI GCNGC 3 cut(s) 10, 1107, 1110
Bme1390I CCNGG 5 cut(s) 34, 268, 681, 794, 846
Bme18I GGWCC 2 cut(s) 271, 995
BmeT110I CYCGRG 1 cut(s) 945
BmgT120I GGNCC 5 cut(s) 271, 443, 869, 976, 995
BmiI GGNNCC 4 cut(s) 272, 273, 791, 977
BmrFI CCNGG 5 cut(s) 34, 268, 681, 794, 846
BmrI ACTGGG 2 cut(s) 461, 876
BmsI GCATC 2 cut(s) 512, 940
BmuI ACTGGG 2 cut(s) 461, 876
BpmI CTGGAG 1 cut(s) 16
Bpu10I CCTNAGC 2 cut(s) 477, 1050
Bpu1102I GCTNAGC 2 cut(s) 411, 537
BpuEI CTTGAG 1 cut(s) 1091
BsaBI GATNNNNATC 1 cut(s) 571
BsaJI CCNNGG 4 cut(s) 267, 679, 680, 845
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse1I ACTGG 3 cut(s) 467, 871, 1126
Bse3DI GCAATG 2 cut(s) 363, 872
Bse8I GATNNNNATC 1 cut(s) 571
BseBI CCWGG 5 cut(s) 34, 268, 681, 794, 846
BseDI CCNNGG 4 cut(s) 267, 679, 680, 845
BseJI GATNNNNATC 1 cut(s) 571
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 2 cut(s) 363, 872
BseMII CTCAG 5 cut(s) 32, 425, 468, 551, 1041
BseNI ACTGG 3 cut(s) 467, 871, 1126
BseRI GAGGAG 2 cut(s) 288, 1128
BseX3I CGGCCG 1 cut(s) 345
BseXI GCAGC 2 cut(s) 20, 1117
Bsh1285I CGRYCG 1 cut(s) 348
BshFI GGCC 6 cut(s) 242, 347, 383, 444, 871, 978
BsiEI CGRYCG 1 cut(s) 348
BsiHKAI GWGCWC 2 cut(s) 412, 829
BsiHKCI CYCGRG 1 cut(s) 945
BsiSI CCGG 1 cut(s) 954
BslFI GGGAC 2 cut(s) 196, 257
BslI CCNNNNNNNGG 1 cut(s) 337
BsmAI GTCTC 3 cut(s) 188, 194, 200
BsmBI CGTCTC 3 cut(s) 188, 194, 200
BsmFI GGGAC 2 cut(s) 196, 257
BsnI GGCC 6 cut(s) 242, 347, 383, 444, 871, 978
BsoBI CYCGRG 1 cut(s) 945
Bsp1286I GDGCHC 3 cut(s) 47, 412, 829
Bsp1407I TGTACA 1 cut(s) 597
Bsp143I GATC 5 cut(s) 24, 566, 589, 807, 1117
Bsp1720I GCTNAGC 2 cut(s) 411, 537
BspACI CCGC 3 cut(s) 219, 246, 1108
BspANI GGCC 6 cut(s) 242, 347, 383, 444, 871, 978
BspCNI CTCAG 5 cut(s) 33, 424, 469, 550, 1042
BspLI GGNNCC 4 cut(s) 272, 273, 791, 977
BspMI ACCTGC 2 cut(s) 286, 949
BspPI GGATC 1 cut(s) 574
BsrDI GCAATG 2 cut(s) 363, 872
BsrGI TGTACA 1 cut(s) 597
BsrI ACTGG 3 cut(s) 467, 871, 1126
BssECI CCNNGG 4 cut(s) 267, 679, 680, 845
BssMI GATC 5 cut(s) 24, 566, 589, 807, 1117
Bst2UI CCWGG 5 cut(s) 34, 268, 681, 794, 846
Bst4CI ACNGT 1 cut(s) 327
Bst6I CTCTTC 1 cut(s) 1026
BstAPI GCANNNNNTGC 1 cut(s) 824
BstAUI TGTACA 1 cut(s) 597
BstC8I GCNNGC 4 cut(s) 88, 376, 393, 1014
BstDEI CTNAG 6 cut(s) 41, 411, 477, 537, 1050, 1076
BstH2I RGCGCY 1 cut(s) 544
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 5 cut(s) 27, 569, 592, 810, 1120
BstMAI GTCTC 3 cut(s) 188, 194, 200
BstMBI GATC 5 cut(s) 24, 566, 589, 807, 1117
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 3 cut(s) 380, 824, 950
BstNI CCWGG 5 cut(s) 34, 268, 681, 794, 846
BstSCI CCNGG 5 cut(s) 32, 266, 679, 792, 844
BstSFI CTRYAG 2 cut(s) 906, 1020
BstV1I GCAGC 2 cut(s) 20, 1117
BstX2I RGATCY 2 cut(s) 566, 1117
BstXI CCANNNNNNTGG 1 cut(s) 879
BstYI RGATCY 2 cut(s) 566, 1117
BstZI CGGCCG 1 cut(s) 345
BsuRI GGCC 6 cut(s) 242, 347, 383, 444, 871, 978
BtgZI GCGATG 1 cut(s) 188
BtsIMutI CAGTG 2 cut(s) 474, 864
BveI ACCTGC 2 cut(s) 286, 949
Cac8I GCNNGC 4 cut(s) 88, 376, 393, 1014
CaiI CAGNNNCTG 1 cut(s) 544
CfoI GCGC 1 cut(s) 543
Cfr13I GGNCC 5 cut(s) 271, 443, 869, 976, 995
CseI GACGC 1 cut(s) 497
Csp6I GTAC 2 cut(s) 598, 621
CviAII CATG 5 cut(s) 371, 520, 859, 972, 1028
CviQI GTAC 2 cut(s) 598, 621
DdeI CTNAG 6 cut(s) 41, 411, 477, 537, 1050, 1076
DpnI GATC 5 cut(s) 26, 568, 591, 809, 1119
DpnII GATC 5 cut(s) 24, 566, 589, 807, 1117
EaeI YGGCCR 1 cut(s) 345
EagI CGGCCG 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 1026
EarI CTCTTC 1 cut(s) 1026
EclXI CGGCCG 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 676
Eco47I GGWCC 2 cut(s) 271, 995
Eco52I CGGCCG 1 cut(s) 345
Eco57I CTGAAG 2 cut(s) 549, 1121
Eco88I CYCGRG 1 cut(s) 945
EcoO109I RGGNCCY 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 1150
EcoRII CCWGG 5 cut(s) 32, 266, 679, 792, 844
EcoRV GATATC 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 47
Esp3I CGTCTC 3 cut(s) 188, 194, 200
FaeI CATG 5 cut(s) 374, 523, 862, 975, 1031
FalI AAGNNNNNCTT 2 cut(s) 677, 709
FaqI GGGAC 2 cut(s) 196, 257
FatI CATG 5 cut(s) 370, 519, 858, 971, 1027
FbaI TGATCA 2 cut(s) 24, 589
Fnu4HI GCNGC 3 cut(s) 9, 1106, 1109
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 3 cut(s) 9, 1106, 1109
FspBI CTAG 1 cut(s) 800
GlaI GCGC 1 cut(s) 542
GluI GCNGC 3 cut(s) 9, 1106, 1109
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 544
HaeIII GGCC 6 cut(s) 242, 347, 383, 444, 871, 978
HapII CCGG 1 cut(s) 954
HgaI GACGC 1 cut(s) 497
HhaI GCGC 1 cut(s) 543
Hin1II CATG 5 cut(s) 374, 523, 862, 975, 1031
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 3 cut(s) 37, 701, 709
HpaII CCGG 1 cut(s) 954
HphI GGTGA 8 cut(s) 117, 154, 208, 352, 509, 544, 599, 930
Hpy166II GTNNAC 3 cut(s) 307, 742, 938
Hpy188I TCNGA 6 cut(s) 24, 42, 529, 577, 995, 1143
Hpy188III TCNNGA 1 cut(s) 947
Hpy8I GTNNAC 3 cut(s) 307, 742, 938
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 4 cut(s) 69, 142, 293, 519
HpyCH4III ACNGT 1 cut(s) 327
HpyCH4V TGCA 2 cut(s) 877, 892
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 824, 950
HpyF3I CTNAG 6 cut(s) 41, 411, 477, 537, 1050, 1076
Hsp92II CATG 5 cut(s) 374, 523, 862, 975, 1031
HspAI GCGC 1 cut(s) 541
KflI GGGWCCC 1 cut(s) 271
Ksp22I TGATCA 2 cut(s) 24, 589
Kzo9I GATC 5 cut(s) 24, 566, 589, 807, 1117
LmnI GCTCC 4 cut(s) 35, 95, 815, 956
Lsp1109I GCAGC 2 cut(s) 20, 1117
LweI GCATC 2 cut(s) 512, 940
MaeI CTAG 1 cut(s) 800
MaeIII GTNAC 1 cut(s) 340
MalI GATC 5 cut(s) 26, 568, 591, 809, 1119
MboI GATC 5 cut(s) 24, 566, 589, 807, 1117
MboII GAAGA 9 cut(s) 54, 63, 66, 69, 272, 664, 697, 1043, 1092
MfeI CAATTG 1 cut(s) 332
MflI RGATCY 2 cut(s) 566, 1117
MhlI GDGCHC 3 cut(s) 47, 412, 829
MluCI AATT 6 cut(s) 172, 223, 332, 884, 1038, 1150
MlyI GAGTC 2 cut(s) 31, 703
MmeI TCCRAC 2 cut(s) 144, 973
MseI TTAA 2 cut(s) 315, 855
MspA1I CMGCKG 3 cut(s) 415, 595, 1108
MspI CCGG 1 cut(s) 954
MspR9I CCNGG 5 cut(s) 34, 268, 681, 794, 846
MunI CAATTG 1 cut(s) 332
MvaI CCWGG 5 cut(s) 34, 268, 681, 794, 846
MwoI GCNNNNNNNGC 3 cut(s) 380, 824, 950
NdeII GATC 5 cut(s) 24, 566, 589, 807, 1117
NlaIII CATG 5 cut(s) 374, 523, 862, 975, 1031
NlaIV GGNNCC 4 cut(s) 272, 273, 791, 977
NmuCI GTSAC 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 945
PaqCI CACCTGC 2 cut(s) 286, 949
PasI CCCWGGG 1 cut(s) 680
PfeI GAWTC 1 cut(s) 701
PfoI TCCNGGA 2 cut(s) 32, 792
PkrI GCNGC 3 cut(s) 10, 1107, 1110
PleI GAGTC 2 cut(s) 31, 703
PpsI GAGTC 2 cut(s) 31, 703
PpuMI RGGWCCY 1 cut(s) 271
Psp5II RGGWCCY 1 cut(s) 271
Psp6I CCWGG 5 cut(s) 32, 266, 679, 792, 844
PspGI CCWGG 5 cut(s) 32, 266, 679, 792, 844
PspN4I GGNNCC 4 cut(s) 272, 273, 791, 977
PspPI GGNCC 5 cut(s) 271, 443, 869, 976, 995
PspPPI RGGWCCY 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 544
PsuI RGATCY 2 cut(s) 566, 1117
PvuII CAGCTG 2 cut(s) 415, 595
RsaI GTAC 2 cut(s) 599, 622
RsaNI GTAC 2 cut(s) 598, 621
SaqAI TTAA 2 cut(s) 315, 855
SatI GCNGC 3 cut(s) 9, 1106, 1109
Sau3AI GATC 5 cut(s) 24, 566, 589, 807, 1117
Sau96I GGNCC 5 cut(s) 271, 443, 869, 976, 995
SchI GAGTC 2 cut(s) 31, 703
ScrFI CCNGG 5 cut(s) 34, 268, 681, 794, 846
SduI GDGCHC 3 cut(s) 47, 412, 829
SfaNI GCATC 2 cut(s) 512, 940
SfcI CTRYAG 2 cut(s) 906, 1020
Sfr274I CTCGAG 1 cut(s) 945
SinI GGWCC 2 cut(s) 271, 995
SlaI CTCGAG 1 cut(s) 945
SmlI CTYRAG 2 cut(s) 945, 1070
SmoI CTYRAG 2 cut(s) 945, 1070
Sse9I AATT 6 cut(s) 172, 223, 332, 884, 1038, 1150
SsiI CCGC 3 cut(s) 219, 246, 1108
SspMI CTAG 1 cut(s) 800
StyD4I CCNGG 5 cut(s) 32, 266, 679, 792, 844
TaaI ACNGT 1 cut(s) 327
TaqI TCGA 5 cut(s) 233, 387, 399, 512, 946
TasI AATT 6 cut(s) 172, 223, 332, 884, 1038, 1150
TatI WGTACW 1 cut(s) 597
TauI GCSGC 1 cut(s) 1111
TfiI GAWTC 1 cut(s) 701
Tru1I TTAA 2 cut(s) 315, 855
Tru9I TTAA 2 cut(s) 315, 855
TscAI CASTG 2 cut(s) 474, 871
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 8, 1105
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 3 cut(s) 17, 124, 1044
TspGWI ACGGA 2 cut(s) 176, 182
TspRI CASTG 2 cut(s) 474, 871
VpaK11BI GGWCC 2 cut(s) 271, 995
XapI RAATTY 1 cut(s) 1150
XhoI CTCGAG 1 cut(s) 945
XspI CTAG 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.