Rorug07G0153300

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003038
Physical Location & Seq
Reverse (-)
12165218 .. 12166101
884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0153300.1

Sequence Viewer

Length: 633 bp
ATGGCTGAAAAGATCAAACAGGTCGTGGCATCAGTAAAAGGCTACACCAGAAAAGATGAAGAACTGCCCACCTACCGAACTGAGGAGGAGCTCAAACGCCAGAAGAGGATCAAATTGTTTGCTTACATTGGTATTTTCATCGTGTTTCAGATCATAGTGATGACCGTGTTTGGTCTCACTGTCATGAAAGTTAAGACTCCGAAGCTCAGATTGGGTGCCATTAGTGTCCAAACACTCAACTCTGTCCCTGCCACACCTTCCTTCGACACAACGTTTGCCACCCAAATCAGGGTCAAGAACACAAATTGGGGTCCATACAAATTTGATGCAAGCGTTGTAACATTCATGTACCAAGGTGCTGCTGTGGGGCAAGTTGCCATTCCAAAGAGCAAGGCTGGAATGCGCTCCACCAAAAAGATCAATGTCGAGGTGAGTTTGAATTCGGAGGCATTGCAAAGCACCAACCTTGGCAGTGATTTGAACAGCGGGGTCTTAACACTCACCAGCCAAGCCAAATTGACCGGAAAGGTGGAATTGATGCTGATAATGAAGAAAAAGAAGTCTGCCAATATGGACTGCACCATGAAATTTGATCTATCCACAAAAACGGTGCAATCTTTGGAATGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 RWP-RK

Protein Analysis

210

Amino Acids

23.3

Weight (kDa)

9.89

Isoelectric Point (pI)

27.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 96 - 194 2.4e-07 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 215
AciI CCGC 1 cut(s) 486
AclI AACGTT 1 cut(s) 272
AclWI GGATC 1 cut(s) 116
AcsI RAATTY 3 cut(s) 320, 439, 587
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 3 cut(s) 82, 288, 289
AgsI TTSAA 2 cut(s) 439, 481
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 2 cut(s) 91, 205
AluI AGCT 2 cut(s) 91, 205
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 1 cut(s) 116
ApeKI GCWGC 1 cut(s) 359
ApoI RAATTY 3 cut(s) 320, 439, 587
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 1 cut(s) 311
AsuHPI GGTGA 2 cut(s) 442, 493
AvaII GGWCC 1 cut(s) 311
BanI GGYRCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 93
BbvI GCAGC 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 179
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
Bme18I GGWCC 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 2 cut(s) 217, 312
BmsI GCATC 3 cut(s) 38, 316, 528
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 352, 466
BsaWI WCCGGW 1 cut(s) 521
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 288, 289
Bse3DI GCAATG 1 cut(s) 449
BseDI CCNNGG 2 cut(s) 352, 466
BseLI CCNNNNNNNGG 3 cut(s) 82, 288, 289
BseMI GCAATG 1 cut(s) 449
BseMII CTCAG 2 cut(s) 72, 220
BseRI GAGGAG 2 cut(s) 98, 101
BseXI GCAGC 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 562
BshNI GGYRCC 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 93
BsiSI CCGG 1 cut(s) 522
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 3 cut(s) 82, 288, 289
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 230
BsmI GAATGC 2 cut(s) 405, 629
Bso31I GGTCTC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 93
Bsp143I GATC 5 cut(s) 12, 108, 150, 417, 592
BspACI CCGC 1 cut(s) 486
BspCNI CTCAG 2 cut(s) 73, 219
BspHI TCATGA 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 217, 312
BspPI GGATC 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 352, 466
BssMI GATC 5 cut(s) 12, 108, 150, 417, 592
BssT1I CCWWGG 2 cut(s) 352, 466
Bst4CI ACNGT 3 cut(s) 166, 181, 610
Bst6I CTCTTC 1 cut(s) 98
BstC8I GCNNGC 1 cut(s) 331
BstDEI CTNAG 2 cut(s) 81, 206
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 5 cut(s) 15, 111, 153, 420, 595
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 5 cut(s) 12, 108, 150, 417, 592
BstV1I GCAGC 1 cut(s) 346
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 2 cut(s) 177, 478
Cac8I GCNNGC 1 cut(s) 331
CciI TCATGA 1 cut(s) 183
CfoI GCGC 1 cut(s) 405
Cfr13I GGNCC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 349
CviAII CATG 3 cut(s) 184, 346, 583
CviJI RGCY 7 cut(s) 5, 42, 91, 205, 395, 507, 512
CviKI_1 RGCY 7 cut(s) 5, 42, 91, 205, 395, 507, 512
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 2 cut(s) 81, 206
DpnI GATC 5 cut(s) 14, 110, 152, 419, 594
DpnII GATC 5 cut(s) 12, 108, 150, 417, 592
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Ecl136II GAGCTC 1 cut(s) 91
Eco130I CCWWGG 2 cut(s) 352, 466
Eco24I GRGCYC 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 311
Eco53kI GAGCTC 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 91
EcoRI GAATTC 1 cut(s) 439
EcoT14I CCWWGG 2 cut(s) 352, 466
EcoT38I GRGCYC 1 cut(s) 93
ErhI CCWWGG 2 cut(s) 352, 466
FaeI CATG 3 cut(s) 187, 349, 586
FaiI YATR 6 cut(s) 155, 185, 316, 347, 572, 584
FaqI GGGAC 1 cut(s) 230
FatI CATG 3 cut(s) 183, 345, 582
FauI CCCGC 1 cut(s) 479
Fnu4HI GCNGC 1 cut(s) 360
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 360
GlaI GCGC 1 cut(s) 404
GluI GCNGC 1 cut(s) 360
HapII CCGG 1 cut(s) 522
HhaI GCGC 1 cut(s) 405
Hin1II CATG 3 cut(s) 187, 349, 586
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HinfI GANTC 1 cut(s) 196
HpaII CCGG 1 cut(s) 522
HphI GGTGA 2 cut(s) 442, 493
Hpy188I TCNGA 4 cut(s) 150, 201, 209, 445
Hpy188III TCNNGA 2 cut(s) 184, 295
HpyAV CCTTC 2 cut(s) 267, 271
HpyCH4III ACNGT 3 cut(s) 166, 181, 610
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 5 cut(s) 329, 454, 579, 613, 627
HpyF3I CTNAG 2 cut(s) 81, 206
HpySE526I ACGT 1 cut(s) 272
Hsp92II CATG 3 cut(s) 187, 349, 586
HspAI GCGC 1 cut(s) 403
Kzo9I GATC 5 cut(s) 12, 108, 150, 417, 592
LmnI GCTCC 2 cut(s) 88, 410
LpnPI CCDG 8 cut(s) 5, 61, 113, 261, 274, 381, 517, 535
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 3 cut(s) 38, 316, 528
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 1 cut(s) 337
MalI GATC 5 cut(s) 14, 110, 152, 419, 594
MboI GATC 5 cut(s) 12, 108, 150, 417, 592
MboII GAAGA 3 cut(s) 71, 115, 562
MhlI GDGCHC 1 cut(s) 93
MluCI AATT 7 cut(s) 113, 304, 320, 439, 515, 533, 587
MlyI GAGTC 1 cut(s) 190
MnlI CCTC 5 cut(s) 76, 79, 99, 421, 439
MseI TTAA 2 cut(s) 192, 494
MslI CAYNNNNRTG 2 cut(s) 158, 182
MspA1I CMGCKG 1 cut(s) 486
MspI CCGG 1 cut(s) 522
Mva1269I GAATGC 2 cut(s) 405, 629
NdeII GATC 5 cut(s) 12, 108, 150, 417, 592
NlaIII CATG 3 cut(s) 187, 349, 586
NlaIV GGNNCC 2 cut(s) 217, 312
PagI TCATGA 1 cut(s) 183
PctI GAATGC 2 cut(s) 405, 629
PkrI GCNGC 1 cut(s) 361
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Psp124BI GAGCTC 1 cut(s) 93
Psp1406I AACGTT 1 cut(s) 272
PspN4I GGNNCC 2 cut(s) 217, 312
PspPI GGNCC 1 cut(s) 311
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 2 cut(s) 158, 182
SacI GAGCTC 1 cut(s) 93
SaqAI TTAA 2 cut(s) 192, 494
SatI GCNGC 1 cut(s) 360
Sau3AI GATC 5 cut(s) 12, 108, 150, 417, 592
Sau96I GGNCC 1 cut(s) 311
SchI GAGTC 1 cut(s) 190
SduI GDGCHC 1 cut(s) 93
SfaNI GCATC 3 cut(s) 38, 316, 528
SinI GGWCC 1 cut(s) 311
SmiMI CAYNNNNRTG 2 cut(s) 158, 182
Sse9I AATT 7 cut(s) 113, 304, 320, 439, 515, 533, 587
SsiI CCGC 1 cut(s) 486
SstI GAGCTC 1 cut(s) 93
StyI CCWWGG 2 cut(s) 352, 466
TaaI ACNGT 3 cut(s) 166, 181, 610
TaiI ACGT 1 cut(s) 275
TaqI TCGA 2 cut(s) 264, 426
TasI AATT 7 cut(s) 113, 304, 320, 439, 515, 533, 587
Tru1I TTAA 2 cut(s) 192, 494
Tru9I TTAA 2 cut(s) 192, 494
TscAI CASTG 2 cut(s) 184, 478
TseI GCWGC 1 cut(s) 359
TspDTI ATGAA 6 cut(s) 72, 127, 200, 334, 563, 599
TspRI CASTG 2 cut(s) 184, 478
VpaK11BI GGWCC 1 cut(s) 311
XapI RAATTY 3 cut(s) 320, 439, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.