Rh1AG000200

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0012705
Physical Location & Seq
Reverse (-)
94023 .. 106145
12123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG000200.1

Sequence Viewer

Length: 474 bp
ATGTGGCCCACTCCGGTGGAAGATGGTGGTGGCTGGCGTGGCAGCGGATTTATCACTGCCCTGGGTTGGCCTGCACTGAAGGAGTTGTCAAATGTCAGCGGTGGGCACTTGAGCGGCTCAACTGACAGTGACACTAATGACGCAAAGAAAATTGGTGCTGAAGAAGATTTTGAGAATTTTCTTGGTTGCTTCAAAGATACGAATATGCAAGCCCAAGGTGTGGATCCCAAGAGGGGTTGGGGATTTCGTGGTGTTCACAAGTTTGAAATCTCACAAGAAGTTGCCAAACTGCGTGCCCAGATAACCACTTTGCAGAACAGTAACAGGGGATATATGGTTGAGGGTTTGAGCAATATGTCTATCAAGGAGCTCAAGGGCGTGGAGACCAAACTTGAGAAAGCAATTAGCAGAATTAGATCCAAGAAGGATTGGAAGATATGTTCACTTAAAGAAAAGCAGTTACTCAAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SSB

Protein Analysis

157

Amino Acids

17.38

Weight (kDa)

9.08

Isoelectric Point (pI)

27.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 89 - 146 5.2e-16 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 220
AccBSI CCGCTC 1 cut(s) 114
AciI CCGC 3 cut(s) 45, 99, 114
AclWI GGATC 3 cut(s) 218, 231, 411
AcsI RAATTY 1 cut(s) 175
AcuI CTGAAG 2 cut(s) 98, 180
AfiI CCNNNNNNNGG 3 cut(s) 66, 220, 233
AgsI TTSAA 2 cut(s) 193, 266
AjnI CCWGG 1 cut(s) 60
AleI CACNNNNGTG 1 cut(s) 14
AloI GAACNNNNNNTCC 2 cut(s) 424, 456
AluBI AGCT 2 cut(s) 370, 470
AluI AGCT 2 cut(s) 370, 470
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 1 cut(s) 377
AlwI GGATC 3 cut(s) 218, 231, 411
AoxI GGCC 2 cut(s) 5, 68
ApeKI GCWGC 1 cut(s) 42
ApoI RAATTY 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 6
BaeGI GKGCMC 2 cut(s) 108, 298
BamHI GGATCC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 372
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 54
BccI CCATC 1 cut(s) 17
BciT130I CCWGG 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 377
BisI GCNGC 2 cut(s) 43, 115
BlsI GCNGC 2 cut(s) 44, 116
Bme1390I CCNGG 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 1 cut(s) 62
BpuEI CTTGAG 3 cut(s) 130, 356, 413
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 3 cut(s) 60, 61, 214
BsaWI WCCGGW 1 cut(s) 13
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 220, 233
BseBI CCWGG 1 cut(s) 62
BseDI CCNNGG 3 cut(s) 60, 61, 214
BseLI CCNNNNNNNGG 3 cut(s) 66, 220, 233
BseSI GKGCMC 2 cut(s) 108, 298
BseXI GCAGC 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 2 cut(s) 7, 70
BsiHKAI GWGCWC 1 cut(s) 372
BsiSI CCGG 1 cut(s) 14
BslI CCNNNNNNNGG 3 cut(s) 66, 220, 233
BsmAI GTCTC 1 cut(s) 377
BsnI GGCC 2 cut(s) 7, 70
Bso31I GGTCTC 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 108, 298, 372
Bsp143I GATC 2 cut(s) 223, 416
BspACI CCGC 3 cut(s) 45, 99, 114
BspANI GGCC 2 cut(s) 7, 70
BspLI GGNNCC 1 cut(s) 225
BspPI GGATC 3 cut(s) 218, 231, 411
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 114
BssECI CCNNGG 3 cut(s) 60, 61, 214
BssMI GATC 2 cut(s) 223, 416
BssT1I CCWWGG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 2 cut(s) 128, 320
BstC8I GCNNGC 4 cut(s) 35, 72, 210, 294
BstDEI CTNAG 1 cut(s) 471
BstKTI GATC 2 cut(s) 226, 419
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 2 cut(s) 223, 416
BstMWI GCNNNNNNNGC 1 cut(s) 39
BstNI CCWGG 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 60
BstSLI GKGCMC 2 cut(s) 108, 298
BstV1I GCAGC 1 cut(s) 54
BstX2I RGATCY 2 cut(s) 223, 416
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 2 cut(s) 223, 416
BsuRI GGCC 2 cut(s) 7, 70
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 3 cut(s) 54, 74, 133
Cac8I GCNNGC 4 cut(s) 35, 72, 210, 294
Cfr13I GGNCC 1 cut(s) 6
CseI GACGC 1 cut(s) 149
CviJI RGCY 7 cut(s) 7, 33, 70, 117, 212, 370, 470
CviKI_1 RGCY 7 cut(s) 7, 33, 70, 117, 212, 370, 470
DdeI CTNAG 1 cut(s) 471
DpnI GATC 2 cut(s) 225, 418
DpnII GATC 2 cut(s) 223, 416
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 2 cut(s) 98, 180
EcoICRI GAGCTC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 214
FaiI YATR 5 cut(s) 206, 333, 335, 356, 439
Fnu4HI GCNGC 2 cut(s) 43, 115
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 2 cut(s) 43, 115
GluI GCNGC 2 cut(s) 43, 115
HaeIII GGCC 2 cut(s) 7, 70
HapII CCGG 1 cut(s) 14
HgaI GACGC 1 cut(s) 149
HindIII AAGCTT 1 cut(s) 468
HpaII CCGG 1 cut(s) 14
Hpy166II GTNNAC 2 cut(s) 256, 443
Hpy8I GTNNAC 2 cut(s) 256, 443
HpyAV CCTTC 2 cut(s) 73, 418
HpyCH4III ACNGT 2 cut(s) 128, 320
HpyCH4V TGCA 3 cut(s) 74, 208, 313
HpyF10VI GCNNNNNNNGC 1 cut(s) 39
HpyF3I CTNAG 1 cut(s) 471
Kzo9I GATC 2 cut(s) 223, 416
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 7 cut(s) 19, 27, 47, 74, 84, 310, 311
Lsp1109I GCAGC 1 cut(s) 54
MaeIII GTNAC 3 cut(s) 128, 320, 459
MalI GATC 2 cut(s) 225, 418
MbiI CCGCTC 1 cut(s) 114
MboI GATC 2 cut(s) 223, 416
MboII GAAGA 4 cut(s) 32, 173, 176, 445
MflI RGATCY 2 cut(s) 223, 416
MhlI GDGCHC 3 cut(s) 108, 298, 372
MluCI AATT 4 cut(s) 150, 175, 402, 411
MnlI CCTC 2 cut(s) 225, 334
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 14
MspA1I CMGCKG 2 cut(s) 45, 99
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 1 cut(s) 62
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 1 cut(s) 39
NdeII GATC 2 cut(s) 223, 416
NlaIV GGNNCC 1 cut(s) 225
NmuCI GTSAC 1 cut(s) 128
OliI CACNNNNGTG 1 cut(s) 14
PasI CCCWGGG 1 cut(s) 61
PflMI CCANNNNNTGG 1 cut(s) 220
PkrI GCNGC 2 cut(s) 44, 116
Psp124BI GAGCTC 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 60
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 225
PspPI GGNCC 1 cut(s) 6
PsuI RGATCY 2 cut(s) 223, 416
RseI CAYNNNNRTG 1 cut(s) 14
SacI GAGCTC 1 cut(s) 372
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 2 cut(s) 43, 115
Sau3AI GATC 2 cut(s) 223, 416
Sau96I GGNCC 1 cut(s) 6
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 3 cut(s) 108, 298, 372
SetI ASST 3 cut(s) 220, 372, 472
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 3 cut(s) 109, 371, 392
SmoI CTYRAG 3 cut(s) 109, 371, 392
Sse9I AATT 4 cut(s) 150, 175, 402, 411
SsiI CCGC 3 cut(s) 45, 99, 114
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 214
TaaI ACNGT 2 cut(s) 128, 320
TasI AATT 4 cut(s) 150, 175, 402, 411
TauI GCSGC 1 cut(s) 117
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 3 cut(s) 61, 81, 133
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 1 cut(s) 128
TspRI CASTG 3 cut(s) 61, 81, 133
Van91I CCANNNNNTGG 1 cut(s) 220
XapI RAATTY 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.