Rh6DG012500

PAP-specific phosphatase HAL2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0012705
Physical Location & Seq
Forward (+)
1216619 .. 1218686
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG012500.1

Sequence Viewer

Length: 624 bp
ATGGAGGAAGATAAGTACGCTAAGGAGTTGGACGTGGCCGTTAGAGTTGTGCACATGGCTTGTGCTCTGTGTCAGAGAGTTCAAGAGGGTTTGGTTTCAGCTAGCACTGACCAGGTGAAGTCCAAAGACGATGATTCCCCTGTAACAATTGCAGATATTGACATGCTGTTGATATCCAGGGGATATATGGCTGAGGGTTTGAGCAATATGTCCATCAAGGAGCTCAAGGGCGTGGAGACCAAACTTGAGAAAGCAATTAGCAGAATTAGATCCAAGAAGGATTGGAAGATATGTTCTCTTAAAGAAAAGCAGTTGTGCATAAAAATAGAAGGGCAAAGGAGGCATGGTGAAAAAGAAAAGGAGAGGAGAGAGGGCAAAGAAAGACGAGACGACAGGGATTTTAACCATGATGGCAGTAGGGACTTCAACATGCAGCGTTTTCCACACAAACAGAAATCCACTCGTAGGAGTGAAGACTTAGCTGCTGACCAATTGCATCAAGGTGGGGAAGACGATGAAAGCCTGGGAGAACATCTCATTTCATCTTCTCATGACGATAAAAATTTTGCAAAAAGTGAGAATTGTGATGTTTTTGTGCAAAGGAGCAATTTTATGAAAACTTAA

Protein Analysis

207

Amino Acids

23.82

Weight (kDa)

6.41

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 60 - 97 5.4e-08 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 264
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 562
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 1 cut(s) 465
AgsI TTSAA 2 cut(s) 83, 427
AjiI CACGTC 1 cut(s) 34
AjnI CCWGG 3 cut(s) 111, 176, 522
AloI GAACNNNNNNTCC 2 cut(s) 277, 309
AluBI AGCT 3 cut(s) 101, 223, 482
AluI AGCT 3 cut(s) 101, 223, 482
Alw21I GWGCWC 3 cut(s) 54, 67, 225
Alw26I GTCTC 2 cut(s) 230, 381
Alw44I GTGCAC 1 cut(s) 50
AlwI GGATC 1 cut(s) 264
AoxI GGCC 1 cut(s) 36
ApaLI GTGCAC 1 cut(s) 50
ApeKI GCWGC 2 cut(s) 433, 482
ApoI RAATTY 1 cut(s) 562
AsuHPI GGTGA 2 cut(s) 127, 359
AsuNHI GCTAGC 1 cut(s) 101
BaeGI GKGCMC 1 cut(s) 54
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 2 cut(s) 480, 516
Bbv12I GWGCWC 3 cut(s) 54, 67, 225
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 445, 469
BccI CCATC 2 cut(s) 221, 404
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 3 cut(s) 113, 178, 524
BcoDI GTCTC 2 cut(s) 230, 381
BfaI CTAG 1 cut(s) 102
BisI GCNGC 2 cut(s) 434, 483
BlsI GCNGC 2 cut(s) 435, 484
Bme1390I CCNGG 3 cut(s) 113, 178, 524
BmgBI CACGTC 1 cut(s) 34
BmrFI CCNGG 3 cut(s) 113, 178, 524
BmsI GCATC 1 cut(s) 505
BmtI GCTAGC 1 cut(s) 105
BpiI GAAGAC 2 cut(s) 480, 516
BplI GAGNNNNNCTC 2 cut(s) 519, 551
Bpu10I CCTNAGC 2 cut(s) 21, 192
BpuEI CTTGAG 2 cut(s) 209, 266
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 177, 523
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 1 cut(s) 465
BseBI CCWGG 3 cut(s) 113, 178, 524
BseDI CCNNGG 2 cut(s) 177, 523
BseLI CCNNNNNNNGG 1 cut(s) 465
BseMII CTCAG 1 cut(s) 183
BseRI GAGGAG 1 cut(s) 379
BseSI GKGCMC 1 cut(s) 54
BseXI GCAGC 2 cut(s) 445, 469
BshFI GGCC 1 cut(s) 38
BsiHKAI GWGCWC 3 cut(s) 54, 67, 225
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 465
BsmAI GTCTC 2 cut(s) 230, 381
BsmBI CGTCTC 1 cut(s) 381
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 1 cut(s) 38
Bso31I GGTCTC 1 cut(s) 230
Bsp1286I GDGCHC 3 cut(s) 54, 67, 225
Bsp143I GATC 1 cut(s) 269
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 184
BspHI TCATGA 1 cut(s) 550
BspOI GCTAGC 1 cut(s) 105
BspPI GGATC 1 cut(s) 264
BspTNI GGTCTC 1 cut(s) 230
BssECI CCNNGG 2 cut(s) 177, 523
BssMI GATC 1 cut(s) 269
Bst2UI CCWGG 3 cut(s) 113, 178, 524
BstC8I GCNNGC 1 cut(s) 103
BstDEI CTNAG 3 cut(s) 21, 192, 478
BstKTI GATC 1 cut(s) 272
BstMAI GTCTC 2 cut(s) 230, 381
BstMBI GATC 1 cut(s) 269
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNI CCWGG 3 cut(s) 113, 178, 524
BstNSI RCATGY 2 cut(s) 166, 433
BstSCI CCNGG 3 cut(s) 111, 176, 522
BstSLI GKGCMC 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 445, 469
BstV2I GAAGAC 2 cut(s) 480, 516
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
BsuRI GGCC 1 cut(s) 38
BtrI CACGTC 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 103
CciI TCATGA 1 cut(s) 550
CsiI ACCWGGT 1 cut(s) 111
Csp6I GTAC 1 cut(s) 16
CviAII CATG 6 cut(s) 55, 163, 344, 407, 430, 551
CviJI RGCY 7 cut(s) 38, 59, 101, 191, 223, 482, 522
CviKI_1 RGCY 7 cut(s) 38, 59, 101, 191, 223, 482, 522
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 3 cut(s) 21, 192, 478
DpnI GATC 1 cut(s) 271
DpnII GATC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 230
Eco32I GATATC 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 223
EcoRII CCWGG 3 cut(s) 111, 176, 522
EcoRV GATATC 1 cut(s) 174
EcoT38I GRGCYC 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 6 cut(s) 58, 166, 347, 410, 433, 554
FaqI GGGAC 1 cut(s) 434
FatI CATG 6 cut(s) 54, 162, 343, 406, 429, 550
Fnu4HI GCNGC 2 cut(s) 434, 483
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 2 cut(s) 434, 483
FspBI CTAG 1 cut(s) 102
GluI GCNGC 2 cut(s) 434, 483
HaeIII GGCC 1 cut(s) 38
Hin1II CATG 6 cut(s) 58, 166, 347, 410, 433, 554
HinfI GANTC 1 cut(s) 134
HphI GGTGA 2 cut(s) 127, 359
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 2 cut(s) 83, 551
Hpy8I GTNNAC 1 cut(s) 52
HpyAV CCTTC 2 cut(s) 271, 323
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 7 cut(s) 52, 152, 318, 433, 496, 569, 598
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 3 cut(s) 21, 192, 478
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 6 cut(s) 58, 166, 347, 410, 433, 554
Kzo9I GATC 1 cut(s) 269
LmnI GCTCC 2 cut(s) 220, 603
LpnPI CCDG 8 cut(s) 98, 125, 153, 163, 190, 379, 509, 536
Lsp1109I GCAGC 2 cut(s) 445, 469
LweI GCATC 1 cut(s) 505
MabI ACCWGGT 1 cut(s) 111
MaeI CTAG 1 cut(s) 102
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 142
MalI GATC 1 cut(s) 271
MboI GATC 1 cut(s) 269
MboII GAAGA 5 cut(s) 20, 298, 485, 521, 537
MfeI CAATTG 2 cut(s) 147, 491
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 3 cut(s) 54, 67, 225
MluCI AATT 7 cut(s) 147, 255, 264, 491, 562, 580, 607
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 5 cut(s) 79, 187, 333, 357, 364
MseI TTAA 3 cut(s) 300, 402, 622
MslI CAYNNNNRTG 1 cut(s) 501
MspR9I CCNGG 3 cut(s) 113, 178, 524
MunI CAATTG 2 cut(s) 147, 491
MvaI CCWGG 3 cut(s) 113, 178, 524
MwoI GCNNNNNNNGC 1 cut(s) 340
NdeII GATC 1 cut(s) 269
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 6 cut(s) 58, 166, 347, 410, 433, 554
NspI RCATGY 2 cut(s) 166, 433
PagI TCATGA 1 cut(s) 550
PfeI GAWTC 1 cut(s) 134
PkrI GCNGC 2 cut(s) 435, 484
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 3 cut(s) 111, 176, 522
PspGI CCWGG 3 cut(s) 111, 176, 522
PsuI RGATCY 1 cut(s) 269
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 501
SacI GAGCTC 1 cut(s) 225
SaqAI TTAA 3 cut(s) 300, 402, 622
SatI GCNGC 2 cut(s) 434, 483
Sau3AI GATC 1 cut(s) 269
ScrFI CCNGG 3 cut(s) 113, 178, 524
SduI GDGCHC 3 cut(s) 54, 67, 225
SetI ASST 6 cut(s) 36, 103, 117, 225, 484, 505
SexAI ACCWGGT 1 cut(s) 111
SfaNI GCATC 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 2 cut(s) 224, 245
SmoI CTYRAG 2 cut(s) 224, 245
Sse9I AATT 7 cut(s) 147, 255, 264, 491, 562, 580, 607
SspMI CTAG 1 cut(s) 102
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 3 cut(s) 111, 176, 522
TaiI ACGT 1 cut(s) 36
TasI AATT 7 cut(s) 147, 255, 264, 491, 562, 580, 607
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 3 cut(s) 300, 402, 622
Tru9I TTAA 3 cut(s) 300, 402, 622
TscAI CASTG 1 cut(s) 112
TseI GCWGC 2 cut(s) 433, 482
TspDTI ATGAA 2 cut(s) 531, 531
TspRI CASTG 1 cut(s) 112
VneI GTGCAC 1 cut(s) 50
XapI RAATTY 1 cut(s) 562
XceI RCATGY 2 cut(s) 166, 433
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.