Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0008783

Physical Location & Seq

Genomic Coordinates Forward (+)

19681416 .. 19683431

Gene Structure 2016 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG115100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGTGGGGTCTTGCTTTTCCTGTTTGCTATGGTCCAACTAGTGCAGTTTTCAAATTTGGTTTTGGATGTGGGAGTGTTTCTATGTACTCTGAACTTAGACTTGCTAGCAATTGTGAGCTCAACAAGGGCTCTTCTTTGGTGTCGGCTTGGGCTTTGGAGGAACAACCTATTGGGAATGAGATTAGTGTAGACAAATCGGAACATGGGTTATTAGTGGAATGTGAGAATAGAGATGTTGTTGTGGAGGGAAGTGAGGGTGGTGGAGATAATGAAGTAGATGCGAGAAGTGGAGGAGGAGGAAATTGGGAGGAGGAGAGTGAGGTTGTTGATGTGCGTGCGCTGGCATCGAGCTTACAGTTTGCGAAAACAGCAGATGATGTGGAAGAGGTTCTGAAGGACAAGGGTAATTTGCCCCTTCAAGTATTTTCATCCATGATCAGGGGTTTTGGAAGAGACAAGTTGATGGATTCCGCATTTGCTGTTGTTGAATGGCTTAAGAGGAGGAAGGAAGAAACTAATAGTATGATTACCCCAAATTTATTTATCTTTAATAGTCTATTGGGTGCAGTGAAGCAGTCTAAACAGTTTGGAGAAATGGATAAAGTCTTGGCTGATATGACGCAGGAAGGGGTGGAACCGAATGTTATAACTTACAATACTATAATGGCAATTTATGTAGAACAAGGACTAAGTACTAAGGCCCTTGATGTTCTTGAGGATATTCAGAAGAAGGGCTTGATTCCATCTTCAGTGACCTACTCTACAGCATTGCTGGCTTATCAGAGAATGCAAGATGGAGTTGGAGCCTTAGAGTTCTTTGTCGAGATCAGAGAAAAGTATCGTAATGGTGATATGTGTAAAGTTTCAGAAGAAGACTGGGAAAGTGAGTTTCTAAAGCTCGAGAACTTTACAAAACGTGTTTGCTACCAAGTGATGCGGCGGTGGCTTGTGATGGATGATAATTTAAGCATCAATGTGCTAAAACTTCTAGTAAAGATGGATAATGCTGGGGTCCCACTTGGTCGGGCAGAACATGAGCGCCTTTTGTGGGCTTGTACCCGTGGAGACCATTATAATGTGGCAAAAGAATTGTATAGTAGGATAAGAGAAAGGCATTCTGAAATAAGTCTATCTGTCTGTAATCACGTTATTTGGGTGATGGGAAAGGCTAAGAAATGGTGGGCAGCTTTGGAGATTTATGAGGATATGTTGGACAAGGGGCCAAAGCCAAATAACATGTCATATGAATTGGTAGTATCTCACTTTAATGTTCTTCTGACTGCTGCTAGAAAAAAGGGTATTTGGAGATGGGGTGTCAGGTTGCTCAACAAGATGGAAGAGAAAGGTCTTAAACCGAGAAGTAAGGAATGGAATGCAGTTCTAGTTGCCTGTTCCAAGGCAGCAGAAACTTCTGCTGCTGTCCAGATTTTTAGAAGAATGGTAGAACAAGGTCAAAAACCCACAATCCTTTCTTATGGGGCCTTGCTCAGTGCCCTTGAAAAGGGGAAACTCTATGATGAGGCTCGCCAGGTGTGGGAACATATGATCAAGGTTGGTGTAAAACCCAACCTGTATGCATACACAATCATGGCCTCAGTTTTCAGTGGACAAGGAAAATTTAATTTGGTAGAGACCATTGTTCAAGAGATGGTTTCATCAGGGATTGAGCCAACGGTAGTCACATACAATGCAATTATTAGTGGCTGTGCACGAAATGATTCAAGCAGTGCTGATGCATATGACTGGTTTGATCGGATGAAAGCTAACAATATTCCTCCAAACAATGTTACCTATGAAATGATGATTGAGGCTCTTGCTAAAGAAGGTAAACCAAGGCTTGCATACGAGTTATATTTGAGGGCTCAAAACCAGGGCATTCACCTCTCTGCAAAGACTTATGATATTTTGGTCCAACCCTCAATGGTTTCTGGAGCTAGTTTTGATCTAAAACTCTTAGGGCCTCGTCCACCTCAGCAGAAAAAGGAAAATTTGCAAGGTAGAAAATCTTCTACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

671

Amino Acids

75.29

Weight (kDa)

5.97

Isoelectric Point (pI)

36.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	176 - 227	1.4e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	178 - 222	2.8e-08	PPR repeat family
PPR_3	PF13812	201 - 260	3.2e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	213 - 257	5.8e-11	PPR repeat family
PPR_long	PF17177	349 - 498	1.9e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	440 - 499	1.2e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	464 - 636	3.5e-13	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	487 - 532	7.8e-08	PPR repeat family
PPR_3	PF13812	513 - 570	5e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	534 - 571	1.8e-06	PPR repeat family
PPR_3	PF13812	546 - 605	2e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	578 - 607	1.3e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008783)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46610 AT3G46610
fragaria_vesca	FvH4_7g06820 FvH4_7g06820 FvH4_7g06820
malus_domestica	MD09G1019500.v1.1 MD17G1020100.v1.1
prunus_persica	Prupe.2G095300_v2.0.a1 Prupe.2G095300_v2.0.a1
pyrus_communis	pycom111g01620
rosa_chinensis	RchiOBHm_Chr1g0336521
rosa_laevigata	RLG00000029357
rosa_multiflora	Rmu_sc0001981.1_g000003 Rmu_sc0005044.1_g000026 Rmu_sc0009324.1_g000001
rosa_roxburghii	Rroxscaffold_4G00315530
rosa_rugosa	Rorug01G0125900
rosa_samantha	Rh1AG147000 Rh1BG115100 Rh1CG138300 Rh1DG153700
rosa_wichuraiana	Rw1G009520 Rw1G011910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	647, 1074
AccI	GTMKAC	1 cut(s)	189
AciI	CCGC	3 cut(s)	471, 937, 940
AcsI	RAATTY	4 cut(s)	53, 535, 1616, 1987
AcuI	CTGAAG	2 cut(s)	413, 732
AfaI	GTAC	3 cut(s)	86, 694, 1057
AfiI	CCNNNNNNNGG	4 cut(s)	438, 1048, 1501, 1534
AflII	CTTAAG	1 cut(s)	494
AflIII	ACRYGT	2 cut(s)	916, 1236
AgsI	TTSAA	6 cut(s)	52, 419, 488, 1499, 1643, 1722
AhlI	ACTAGT	1 cut(s)	38
AjnI	CCWGG	2 cut(s)	1527, 1869
AjuI	GAANNNNNNNTTGG	2 cut(s)	153, 185
AluBI	AGCT	6 cut(s)	118, 351, 898, 1187, 1763, 1934
AluI	AGCT	6 cut(s)	118, 351, 898, 1187, 1763, 1934
Alw21I	GWGCWC	2 cut(s)	120, 1711
Alw26I	GTCTC	3 cut(s)	447, 1059, 1625
Alw44I	GTGCAC	1 cut(s)	1707
Ama87I	CYCGRG	1 cut(s)	899
AoxI	GGCC	5 cut(s)	699, 1220, 1479, 1590, 1958
ApaLI	GTGCAC	1 cut(s)	1707
ApeKI	GCWGC	4 cut(s)	1184, 1283, 1400, 1415
ApoI	RAATTY	4 cut(s)	53, 535, 1616, 1987
ArsI	GACNNNNNNTTYG	2 cut(s)	1223, 1255
Asp700I	GAANNNNTTC	3 cut(s)	387, 1717, 2005
AspLEI	GCGC	2 cut(s)	340, 1041
AspS9I	GGNCC	7 cut(s)	32, 700, 1012, 1220, 1479, 1909, 1958
AsuHPI	GGTGA	3 cut(s)	860, 1168, 1871
AsuNHI	GCTAGC	1 cut(s)	104
AvaI	CYCGRG	1 cut(s)	899
AvaII	GGWCC	3 cut(s)	32, 1012, 1909
BaeGI	GKGCMC	2 cut(s)	1495, 1711
BanII	GRGCYC	3 cut(s)	120, 131, 1864
BarI	GAAGNNNNNNTAC	1 cut(s)	1990
BbsI	GAAGAC	1 cut(s)	879
Bbv12I	GWGCWC	2 cut(s)	120, 1711
BbvCI	CCTCAGC	1 cut(s)	1971
BbvI	GCAGC	4 cut(s)	1196, 1270, 1402, 1412
BccI	CCATC	9 cut(s)	457, 751, 788, 946, 991, 1153, 1302, 1327, 1642
BciT130I	CCWGG	2 cut(s)	1529, 1871
BclI	TGATCA	2 cut(s)	435, 1545
BcoDI	GTCTC	3 cut(s)	447, 1059, 1625
BcuI	ACTAGT	1 cut(s)	38
BfaI	CTAG	6 cut(s)	39, 105, 989, 1287, 1382, 1935
BfmI	CTRYAG	1 cut(s)	762
BfoI	RGCGCY	1 cut(s)	1042
BfrI	CTTAAG	1 cut(s)	494
BisI	GCNGC	5 cut(s)	938, 1185, 1284, 1401, 1416
BlsI	GCNGC	5 cut(s)	939, 1186, 1285, 1402, 1417
BmcAI	AGTACT	1 cut(s)	694
Bme1390I	CCNGG	2 cut(s)	1529, 1871
Bme18I	GGWCC	3 cut(s)	32, 1012, 1909
BmeT110I	CYCGRG	1 cut(s)	899
BmgT120I	GGNCC	7 cut(s)	32, 700, 1012, 1220, 1479, 1909, 1958
BmiI	GGNNCC	6 cut(s)	636, 805, 1013, 1014, 1221, 1480
BmrFI	CCNGG	2 cut(s)	1529, 1871
BmrI	ACTGGG	1 cut(s)	886
BmsI	GCATC	5 cut(s)	268, 353, 924, 978, 1723
BmtI	GCTAGC	1 cut(s)	108
BmuI	ACTGGG	1 cut(s)	886
BpiI	GAAGAC	1 cut(s)	879
BpmI	CTGGAG	1 cut(s)	1950
Bpu10I	CCTNAGC	1 cut(s)	1971
BpuEI	CTTGAG	1 cut(s)	734
BsaI	GGTCTC	2 cut(s)	1059, 1625
BsaJI	CCNNGG	4 cut(s)	1060, 1395, 1832, 1870
BsaXI	ACNNNNNCTCC	2 cut(s)	1297, 1327
Bsc4I	CCNNNNNNNGG	4 cut(s)	438, 1048, 1501, 1534
Bse1I	ACTGG	2 cut(s)	881, 1748
Bse3DI	GCAATG	1 cut(s)	767
BseBI	CCWGG	2 cut(s)	1529, 1871
BseDI	CCNNGG	4 cut(s)	1060, 1395, 1832, 1870
BseGI	GGATG	4 cut(s)	71, 428, 961, 1761
BseLI	CCNNNNNNNGG	4 cut(s)	438, 1048, 1501, 1534
BseMI	GCAATG	1 cut(s)	767
BseMII	CTCAG	3 cut(s)	1501, 1608, 1985
BseNI	ACTGG	2 cut(s)	881, 1748
BseRI	GAGGAG	5 cut(s)	306, 309, 323, 326, 514
BseSI	GKGCMC	2 cut(s)	1495, 1711
BseXI	GCAGC	4 cut(s)	1196, 1270, 1402, 1412
BseYI	CCCAGC	1 cut(s)	1007
BsgI	GTGCAG	2 cut(s)	63, 585
BshFI	GGCC	5 cut(s)	701, 1222, 1481, 1592, 1960
BsiHKAI	GWGCWC	2 cut(s)	120, 1711
BsiHKCI	CYCGRG	1 cut(s)	899
BslFI	GGGAC	1 cut(s)	998
BslI	CCNNNNNNNGG	4 cut(s)	438, 1048, 1501, 1534
BsmAI	GTCTC	3 cut(s)	447, 1059, 1625
BsmFI	GGGAC	1 cut(s)	998
BsmI	GAATGC	4 cut(s)	792, 1114, 1378, 1875
BsnI	GGCC	5 cut(s)	701, 1222, 1481, 1592, 1960
Bso31I	GGTCTC	2 cut(s)	1059, 1625
BsoBI	CYCGRG	1 cut(s)	899
Bsp1286I	GDGCHC	5 cut(s)	120, 131, 1495, 1711, 1864
Bsp143I	GATC	5 cut(s)	435, 825, 1545, 1750, 1942
BspACI	CCGC	3 cut(s)	471, 937, 940
BspANI	GGCC	5 cut(s)	701, 1222, 1481, 1592, 1960
BspCNI	CTCAG	3 cut(s)	1500, 1607, 1984
BspLI	GGNNCC	6 cut(s)	636, 805, 1013, 1014, 1221, 1480
BspOI	GCTAGC	1 cut(s)	108
BspQI	GCTCTTC	1 cut(s)	136
BspTI	CTTAAG	1 cut(s)	494
BspTNI	GGTCTC	2 cut(s)	1059, 1625
BsrDI	GCAATG	1 cut(s)	767
BsrI	ACTGG	2 cut(s)	881, 1748
BssECI	CCNNGG	4 cut(s)	1060, 1395, 1832, 1870
BssMI	GATC	5 cut(s)	435, 825, 1545, 1750, 1942
BssT1I	CCWWGG	2 cut(s)	1395, 1832
Bst2UI	CCWGG	2 cut(s)	1529, 1871
Bst4CI	ACNGT	3 cut(s)	357, 585, 1675
Bst6I	CTCTTC	4 cut(s)	136, 378, 445, 1332
BstAFI	CTTAAG	1 cut(s)	494
BstC8I	GCNNGC	6 cut(s)	106, 336, 342, 774, 1525, 1839
BstDEI	CTNAG	9 cut(s)	95, 689, 696, 808, 1170, 1487, 1594, 1954, 1971
BstDSI	CCRYGG	1 cut(s)	1060
BstENI	CCTNNNNNAGG	1 cut(s)	1499
BstF5I	GGATG	4 cut(s)	71, 428, 961, 1761
BstH2I	RGCGCY	1 cut(s)	1042
BstHHI	GCGC	2 cut(s)	340, 1041
BstKTI	GATC	5 cut(s)	438, 828, 1548, 1753, 1945
BstMAI	GTCTC	3 cut(s)	447, 1059, 1625
BstMBI	GATC	5 cut(s)	435, 825, 1545, 1750, 1942
BstMWI	GCNNNNNNNGC	3 cut(s)	368, 773, 943
BstNI	CCWGG	2 cut(s)	1529, 1871
BstNSI	RCATGY	1 cut(s)	1240
BstSCI	CCNGG	2 cut(s)	1527, 1869
BstSFI	CTRYAG	1 cut(s)	762
BstSLI	GKGCMC	2 cut(s)	1495, 1711
BstV1I	GCAGC	4 cut(s)	1196, 1270, 1402, 1412
BstV2I	GAAGAC	1 cut(s)	879
BsuRI	GGCC	5 cut(s)	701, 1222, 1481, 1592, 1960
BtgI	CCRYGG	1 cut(s)	1060
BtsCI	GGATG	4 cut(s)	71, 428, 961, 1761
BtsI	GCAGTG	2 cut(s)	573, 1732
BtsIMutI	CAGTG	5 cut(s)	573, 756, 1495, 1609, 1732
Cac8I	GCNNGC	6 cut(s)	106, 336, 342, 774, 1525, 1839
CfoI	GCGC	2 cut(s)	340, 1041
Cfr13I	GGNCC	7 cut(s)	32, 700, 1012, 1220, 1479, 1909, 1958
CseI	GACGC	1 cut(s)	628
Csp6I	GTAC	3 cut(s)	85, 693, 1056
CviAII	CATG	5 cut(s)	203, 433, 1034, 1237, 1588
CviQI	GTAC	3 cut(s)	85, 693, 1056
DdeI	CTNAG	9 cut(s)	95, 689, 696, 808, 1170, 1487, 1594, 1954, 1971
DpnI	GATC	5 cut(s)	437, 827, 1547, 1752, 1944
DpnII	GATC	5 cut(s)	435, 825, 1545, 1750, 1942
Eam1104I	CTCTTC	4 cut(s)	136, 378, 445, 1332
EarI	CTCTTC	4 cut(s)	136, 378, 445, 1332
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	2 cut(s)	1395, 1832
Eco24I	GRGCYC	3 cut(s)	120, 131, 1864
Eco31I	GGTCTC	2 cut(s)	1059, 1625
Eco47I	GGWCC	3 cut(s)	32, 1012, 1909
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	2 cut(s)	413, 732
Eco88I	CYCGRG	1 cut(s)	899
EcoICRI	GAGCTC	1 cut(s)	118
EcoNI	CCTNNNNNAGG	1 cut(s)	1499
EcoO109I	RGGNCCY	4 cut(s)	700, 1012, 1479, 1958
EcoRII	CCWGG	2 cut(s)	1527, 1869
EcoT14I	CCWWGG	2 cut(s)	1395, 1832
EcoT22I	ATGCAT	2 cut(s)	1579, 1738
EcoT38I	GRGCYC	3 cut(s)	120, 131, 1864
ErhI	CCWWGG	2 cut(s)	1395, 1832
FaeI	CATG	5 cut(s)	206, 436, 1037, 1240, 1591
FalI	AAGNNNNNCTT	2 cut(s)	719, 751
FaqI	GGGAC	1 cut(s)	998
FatI	CATG	5 cut(s)	202, 432, 1033, 1236, 1587
FauNDI	CATATG	3 cut(s)	1243, 1542, 1738
FbaI	TGATCA	2 cut(s)	435, 1545
FblI	GTMKAC	1 cut(s)	189
Fnu4HI	GCNGC	5 cut(s)	938, 1185, 1284, 1401, 1416
FokI	GGATG	4 cut(s)	78, 415, 968, 1768
FriOI	GRGCYC	3 cut(s)	120, 131, 1864
Fsp4HI	GCNGC	5 cut(s)	938, 1185, 1284, 1401, 1416
FspBI	CTAG	6 cut(s)	39, 105, 989, 1287, 1382, 1935
GlaI	GCGC	2 cut(s)	339, 1040
GluI	GCNGC	5 cut(s)	938, 1185, 1284, 1401, 1416
GsaI	CCCAGC	1 cut(s)	1011
GsuI	CTGGAG	1 cut(s)	1950
HaeII	RGCGCY	1 cut(s)	1042
HaeIII	GGCC	5 cut(s)	701, 1222, 1481, 1592, 1960
HgaI	GACGC	1 cut(s)	628
HhaI	GCGC	2 cut(s)	340, 1041
Hin1II	CATG	5 cut(s)	206, 436, 1037, 1240, 1591
Hin6I	GCGC	2 cut(s)	338, 1039
HinP1I	GCGC	2 cut(s)	338, 1039
HinfI	GANTC	3 cut(s)	467, 739, 1718
HphI	GGTGA	3 cut(s)	860, 1168, 1871
Hpy166II	GTNNAC	5 cut(s)	190, 1607, 1709, 1829, 1967
Hpy188III	TCNNGA	6 cut(s)	713, 823, 901, 1423, 1643, 1929
Hpy8I	GTNNAC	5 cut(s)	190, 1607, 1709, 1829, 1967
HpyAV	CCTTC	6 cut(s)	388, 425, 499, 620, 724, 1817
HpyCH4III	ACNGT	3 cut(s)	357, 585, 1675
HpyCH4IV	ACGT	2 cut(s)	916, 1146
HpyF10VI	GCNNNNNNNGC	3 cut(s)	368, 773, 943
HpyF3I	CTNAG	9 cut(s)	95, 689, 696, 808, 1170, 1487, 1594, 1954, 1971
HpySE526I	ACGT	2 cut(s)	916, 1146
Hsp92II	CATG	5 cut(s)	206, 436, 1037, 1240, 1591
HspAI	GCGC	2 cut(s)	338, 1039
KflI	GGGWCCC	1 cut(s)	1012
Ksp22I	TGATCA	2 cut(s)	435, 1545
Kzo9I	GATC	5 cut(s)	435, 825, 1545, 1750, 1942
LguI	GCTCTTC	1 cut(s)	136
LmnI	GCTCC	2 cut(s)	803, 1931
Lsp1109I	GCAGC	4 cut(s)	1196, 1270, 1402, 1412
LweI	GCATC	5 cut(s)	268, 353, 924, 978, 1723
MaeI	CTAG	6 cut(s)	39, 105, 989, 1287, 1382, 1935
MaeII	ACGT	2 cut(s)	916, 1146
MaeIII	GTNAC	3 cut(s)	751, 1678, 1786
MalI	GATC	5 cut(s)	437, 827, 1547, 1752, 1944
MboI	GATC	5 cut(s)	435, 825, 1545, 1750, 1942
MfeI	CAATTG	1 cut(s)	109
MhlI	GDGCHC	5 cut(s)	120, 131, 1495, 1711, 1864
MmeI	TCCRAC	4 cut(s)	59, 781, 1191, 1936
Mph1103I	ATGCAT	2 cut(s)	1579, 1738
MroXI	GAANNNNTTC	3 cut(s)	387, 1717, 2005
MseI	TTAA	6 cut(s)	495, 549, 965, 1266, 1350, 1620
MslI	CAYNNNNRTG	4 cut(s)	974, 1074, 1266, 1586
MspCI	CTTAAG	1 cut(s)	494
MspR9I	CCNGG	2 cut(s)	1529, 1871
MunI	CAATTG	1 cut(s)	109
Mva1269I	GAATGC	4 cut(s)	792, 1114, 1378, 1875
MvaI	CCWGG	2 cut(s)	1529, 1871
MwoI	GCNNNNNNNGC	3 cut(s)	368, 773, 943
NdeI	CATATG	3 cut(s)	1243, 1542, 1738
NdeII	GATC	5 cut(s)	435, 825, 1545, 1750, 1942
NheI	GCTAGC	1 cut(s)	104
NlaIII	CATG	5 cut(s)	206, 436, 1037, 1240, 1591
NlaIV	GGNNCC	6 cut(s)	636, 805, 1013, 1014, 1221, 1480
NmuCI	GTSAC	2 cut(s)	751, 1678
NsiI	ATGCAT	2 cut(s)	1579, 1738
NspI	RCATGY	1 cut(s)	1240
PaeR7I	CTCGAG	1 cut(s)	899
PciI	ACATGT	1 cut(s)	1236
PciSI	GCTCTTC	1 cut(s)	136
PctI	GAATGC	4 cut(s)	792, 1114, 1378, 1875
PdmI	GAANNNNTTC	3 cut(s)	387, 1717, 2005
PfeI	GAWTC	3 cut(s)	467, 739, 1718
PkrI	GCNGC	5 cut(s)	939, 1186, 1285, 1402, 1417
PpuMI	RGGWCCY	1 cut(s)	1012
PscI	ACATGT	1 cut(s)	1236
PsiI	TTATAA	2 cut(s)	647, 1074
Psp124BI	GAGCTC	1 cut(s)	120
Psp5II	RGGWCCY	1 cut(s)	1012
Psp6I	CCWGG	2 cut(s)	1527, 1869
PspFI	CCCAGC	1 cut(s)	1007
PspGI	CCWGG	2 cut(s)	1527, 1869
PspN4I	GGNNCC	6 cut(s)	636, 805, 1013, 1014, 1221, 1480
PspPI	GGNCC	7 cut(s)	32, 700, 1012, 1220, 1479, 1909, 1958
PspPPI	RGGWCCY	1 cut(s)	1012
RsaI	GTAC	3 cut(s)	86, 694, 1057
RsaNI	GTAC	3 cut(s)	85, 693, 1056
RseI	CAYNNNNRTG	4 cut(s)	974, 1074, 1266, 1586
SacI	GAGCTC	1 cut(s)	120
SapI	GCTCTTC	1 cut(s)	136
SaqAI	TTAA	6 cut(s)	495, 549, 965, 1266, 1350, 1620
SatI	GCNGC	5 cut(s)	938, 1185, 1284, 1401, 1416
Sau3AI	GATC	5 cut(s)	435, 825, 1545, 1750, 1942
Sau96I	GGNCC	7 cut(s)	32, 700, 1012, 1220, 1479, 1909, 1958
ScaI	AGTACT	1 cut(s)	694
ScrFI	CCNGG	2 cut(s)	1529, 1871
SduI	GDGCHC	5 cut(s)	120, 131, 1495, 1711, 1864
SfaNI	GCATC	5 cut(s)	268, 353, 924, 978, 1723
SfcI	CTRYAG	1 cut(s)	762
Sfr274I	CTCGAG	1 cut(s)	899
SinI	GGWCC	3 cut(s)	32, 1012, 1909
SlaI	CTCGAG	1 cut(s)	899
SmiMI	CAYNNNNRTG	4 cut(s)	974, 1074, 1266, 1586
SmlI	CTYRAG	3 cut(s)	494, 713, 899
SmoI	CTYRAG	3 cut(s)	494, 713, 899
SpeI	ACTAGT	1 cut(s)	38
SsiI	CCGC	3 cut(s)	471, 937, 940
SspI	AATATT	1 cut(s)	1771
SspMI	CTAG	6 cut(s)	39, 105, 989, 1287, 1382, 1935
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	2 cut(s)	1527, 1869
StyI	CCWWGG	2 cut(s)	1395, 1832
TaaI	ACNGT	3 cut(s)	357, 585, 1675
TaiI	ACGT	2 cut(s)	919, 1149
TaqI	TCGA	3 cut(s)	347, 822, 900
TatI	WGTACW	2 cut(s)	84, 692
TauI	GCSGC	1 cut(s)	940
TfiI	GAWTC	3 cut(s)	467, 739, 1718
Tru1I	TTAA	6 cut(s)	495, 549, 965, 1266, 1350, 1620
Tru9I	TTAA	6 cut(s)	495, 549, 965, 1266, 1350, 1620
TscAI	CASTG	5 cut(s)	573, 756, 1495, 1609, 1732
TseFI	GTSAC	2 cut(s)	751, 1678
TseI	GCWGC	4 cut(s)	1184, 1283, 1400, 1415
Tsp45I	GTSAC	2 cut(s)	751, 1678
TspDTI	ATGAA	6 cut(s)	285, 417, 1260, 1644, 1772, 1809
TspRI	CASTG	5 cut(s)	573, 756, 1495, 1609, 1732
Vha464I	CTTAAG	1 cut(s)	494
VneI	GTGCAC	1 cut(s)	1707
VpaK11BI	GGWCC	3 cut(s)	32, 1012, 1909
XagI	CCTNNNNNAGG	1 cut(s)	1499
XapI	RAATTY	4 cut(s)	53, 535, 1616, 1987
XceI	RCATGY	1 cut(s)	1240
XhoI	CTCGAG	1 cut(s)	899
XmiI	GTMKAC	1 cut(s)	189
XmnI	GAANNNNTTC	3 cut(s)	387, 1717, 2005
XspI	CTAG	6 cut(s)	39, 105, 989, 1287, 1382, 1935
ZrmI	AGTACT	1 cut(s)	694
Zsp2I	ATGCAT	2 cut(s)	1579, 1738

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.