Rw1G009520

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008783
Physical Location & Seq
Forward (+)
20954193 .. 20964965
10773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G009520.1

Sequence Viewer

Length: 1776 bp
ATGCCAGAACATTCAATATCCTCCAGAGCCATACAATTCCCAAGAGAAGAGATTAGCCAGTCACAGGGCGTAGCCGAGCCGAGTTTGCCATCCCGGGTCGGACTAGTCCGATGGTCAGACCGCCCGACCGGAACCGACCGAAAGGCCTTTAGTTTGAAACTGCTGGTGCTTAGAATTGAATGGCTGGGAGAAAACCACAACATCACTGAGAAACAAGACCACAACTCAACTCAGCTGGCTTTGCAATTCTGGAGTTTCAGGTACTCTGAACTTAGACTTGCTAGCAATTGTGAGCCCAACAAGGGCTCTTCTTTTGTGTCGGCTTGGGCTTTGGAGGAACAAGCTATTGGGAATGAGATTAGTGTAGACAAATCGCAACATGGGTTGTTAGGGGAATGTGAGAGCAAGGATGTTGTTGTGGAGGGAATTGAGGAGAGTGAAATTGTTGATGTGCGTGCGCTGGCATCGAGCTTACAGTTTGCGAAAACAGCAGATGATGTGGAAGAGGTTCTGAAGGACAAGGGTAATTTGCCTCTTCAAGTATTCTCATCCATGATCAGGGGTTTTGGAAGAGACAAGTTGATGGATTCCGCGTTTGCTGTTGTTGAATGGCTTAAGAGGAGGAAGGAAGAAACTAATGGTATGATTACCCCAAATTTATTTATCTTTAATAGTCTATTGGGTGCAGTGAAGCAATCTAAACAGTTTGGAGAAATGGATAAAGTCTTGGCTGATATGACGCAGGAAGGGGTGGAACCGAATGTTATAACTTACAATACTATAATGGCAATTTATGTAGAACAAGGACTAAGTAGTAAGGCCCTTGATGTTCTTGAGGATATTCAGAAGAAGGGCTTGATTCCATCTTCAGTGACCTACTCTACAGCATTGCTGGCTTATCAGAGAATGCAAGATGGAGTTGGAGCCTTAGAGTTCTTTGTCAAGGTCAGAGAAAAGTATCGTAATGGTGATATATGTAAAGTTTCAGAAGAAGACTGGGAAAATGAATTTCTAAAGCTCGAGAACTTTACAAAACGTGTTTGCTACCAAGTGATGCGGCGGTGGCTTGTGGTGGATGATAATTTAAGCATCAGTGTGCTAAAACTTCTAGTAAAGATGGATAATGCGGGGAGAAAAAAGGGAATTTGGAGATGGGGTGTCAGGTTGCTCAACAAGATGGAAGAGAAAGGTCTTAAACCAAGAAGTAAGGAATGGAATGCAGTTCTAGTTGCCTGTTCTAAGGCTGCAGAAACTTCTGCTGCTGTTCAGATTTTTAGAAGAATGGTAGAACAAGGTCAAAAACCCACAATCCTTTCTTATGGGGCCTTGCTCAGTGCTCTTGAAAAGGGGAAACTCTATGATGAGGCTCACCAGGTGTGGGAACATATGATCAAGGTTGGTGTAAAACCCAACCTGTATGCGTACACAATCATGGCCTCAGTTTTCAGTGGACAAGGAAAATTTAATTTGGTGGAGACCATTGTTCAAGAGATGGTTTCATCAGGGATTGAGCCAACGGTAGTCACATACAATGCAATTATTAGTGGCTGTGCACGAAATGATTCAAGCAGTGCTGATGCATATGACTGGTTTGATCGGATGAAAGCTAACAATATTCCTCCAAACAATGTTACTTATGAAATGATGATCGAGGCTCTTGCTAAAGAAGATTCTAGAATATATCCTGTGGAGGTTGGATTTCTAAGAAATCAGTTATGTAGAGGAATGGTGGATAGTCTTGTCCTAGCCATTTCTGTCTTCACTCTGAGGCTATGA

Protein Analysis

591

Amino Acids

66.63

Weight (kDa)

5.82

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 216 - 267 1.4e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 218 - 262 2.4e-08 PPR repeat family
PPR_3 PF13812 241 - 300 2.6e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 253 - 297 4e-11 PPR repeat family
PPR_long PF17177 366 - 470 5.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 388 - 447 9.9e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 435 - 480 3e-08 PPR repeat family
PPR_long PF17177 454 - 555 2.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 461 - 518 4.2e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 482 - 519 1.5e-06 PPR repeat family
PPR_3 PF13812 494 - 553 1.7e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 526 - 555 9.8e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 767
AccI GTMKAC 1 cut(s) 366
AccII CGCG 1 cut(s) 593
AciI CCGC 5 cut(s) 121, 591, 1057, 1060, 1127
AcsI RAATTY 4 cut(s) 655, 1007, 1143, 1460
AcuI CTGAAG 2 cut(s) 533, 852
AdeI CACNNNGTG 1 cut(s) 1375
AfaI GTAC 2 cut(s) 263, 1424
AfiI CCNNNNNNNGG 4 cut(s) 64, 302, 558, 1378
AflII CTTAAG 1 cut(s) 614
AflIII ACRYGT 1 cut(s) 1036
AgsI TTSAA 8 cut(s) 15, 157, 179, 539, 608, 1343, 1487, 1566
AhlI ACTAGT 1 cut(s) 103
AjnI CCWGG 1 cut(s) 1371
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AluBI AGCT 5 cut(s) 235, 344, 471, 1018, 1607
AluI AGCT 5 cut(s) 235, 344, 471, 1018, 1607
Alw21I GWGCWC 2 cut(s) 1339, 1555
Alw26I GTCTC 2 cut(s) 567, 1469
Alw44I GTGCAC 1 cut(s) 1551
Ama87I CYCGRG 2 cut(s) 93, 1019
AoxI GGCC 4 cut(s) 144, 819, 1323, 1434
ApaLI GTGCAC 1 cut(s) 1551
ApeKI GCWGC 2 cut(s) 1244, 1259
ApoI RAATTY 4 cut(s) 655, 1007, 1143, 1460
Asp700I GAANNNNTTC 2 cut(s) 507, 1561
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 2 cut(s) 820, 1323
AsuC2I CCSGG 2 cut(s) 94, 95
AsuHPI GGTGA 2 cut(s) 980, 1361
AsuNHI GCTAGC 1 cut(s) 281
AvaI CYCGRG 2 cut(s) 93, 1019
BaeGI GKGCMC 1 cut(s) 1555
BanII GRGCYC 2 cut(s) 297, 308
BbsI GAAGAC 2 cut(s) 999, 1750
Bbv12I GWGCWC 2 cut(s) 1339, 1555
BbvI GCAGC 2 cut(s) 1231, 1246
BccI CCATC 9 cut(s) 97, 105, 577, 871, 908, 1111, 1146, 1171, 1486
BcgI CGANNNNNNTGC 2 cut(s) 1640, 1674
BciT130I CCWGG 1 cut(s) 1373
BclI TGATCA 2 cut(s) 555, 1389
BcnI CCSGG 2 cut(s) 94, 95
BcoDI GTCTC 2 cut(s) 567, 1469
BcuI ACTAGT 1 cut(s) 103
BfaI CTAG 6 cut(s) 104, 282, 1109, 1226, 1674, 1745
BfmI CTRYAG 2 cut(s) 882, 1245
BfrI CTTAAG 1 cut(s) 614
BisI GCNGC 3 cut(s) 1058, 1245, 1260
BlsI GCNGC 3 cut(s) 1059, 1246, 1261
Bme1390I CCNGG 3 cut(s) 94, 95, 1373
BmeT110I CYCGRG 2 cut(s) 93, 1019
BmgT120I GGNCC 2 cut(s) 820, 1323
BmiI GGNNCC 4 cut(s) 133, 756, 925, 1324
BmrFI CCNGG 3 cut(s) 94, 95, 1373
BmrI ACTGGG 1 cut(s) 1006
BmsI GCATC 4 cut(s) 473, 1044, 1098, 1567
BmtI GCTAGC 1 cut(s) 285
BmuI ACTGGG 1 cut(s) 1006
BpiI GAAGAC 2 cut(s) 999, 1750
BpmI CTGGAG 2 cut(s) 7, 271
BpuEI CTTGAG 1 cut(s) 854
BpuMI CCSGG 2 cut(s) 94, 95
BsaI GGTCTC 1 cut(s) 1469
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 1141, 1171
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 302, 558, 1378
Bse1I ACTGG 3 cut(s) 58, 1001, 1592
Bse3DI GCAATG 1 cut(s) 887
BseBI CCWGG 1 cut(s) 1373
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 5 cut(s) 89, 415, 548, 1081, 1605
BseLI CCNNNNNNNGG 4 cut(s) 64, 302, 558, 1378
BseMI GCAATG 1 cut(s) 887
BseMII CTCAG 5 cut(s) 198, 245, 1345, 1452, 1757
BseNI ACTGG 3 cut(s) 58, 1001, 1592
BseRI GAGGAG 2 cut(s) 446, 634
BseSI GKGCMC 1 cut(s) 1555
BseXI GCAGC 2 cut(s) 1231, 1246
BseYI CCCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 705
Bsh1236I CGCG 1 cut(s) 593
Bsh1285I CGRYCG 2 cut(s) 129, 139
BshFI GGCC 4 cut(s) 146, 821, 1325, 1436
BsiEI CGRYCG 2 cut(s) 129, 139
BsiHKAI GWGCWC 2 cut(s) 1339, 1555
BsiHKCI CYCGRG 2 cut(s) 93, 1019
BsiSI CCGG 2 cut(s) 94, 129
BslI CCNNNNNNNGG 4 cut(s) 64, 302, 558, 1378
BsmAI GTCTC 2 cut(s) 567, 1469
BsmI GAATGC 2 cut(s) 912, 1222
BsnI GGCC 4 cut(s) 146, 821, 1325, 1436
Bso31I GGTCTC 1 cut(s) 1469
BsoBI CYCGRG 2 cut(s) 93, 1019
Bsp1286I GDGCHC 4 cut(s) 297, 308, 1339, 1555
Bsp143I GATC 4 cut(s) 555, 1389, 1594, 1647
BspACI CCGC 5 cut(s) 121, 591, 1057, 1060, 1127
BspANI GGCC 4 cut(s) 146, 821, 1325, 1436
BspCNI CTCAG 5 cut(s) 199, 244, 1344, 1451, 1758
BspFNI CGCG 1 cut(s) 593
BspLI GGNNCC 4 cut(s) 133, 756, 925, 1324
BspMAI CTGCAG 1 cut(s) 1249
BspOI GCTAGC 1 cut(s) 285
BspQI GCTCTTC 1 cut(s) 313
BspTI CTTAAG 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 1469
BsrDI GCAATG 1 cut(s) 887
BsrI ACTGG 3 cut(s) 58, 1001, 1592
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 4 cut(s) 555, 1389, 1594, 1647
Bst2UI CCWGG 1 cut(s) 1373
Bst4CI ACNGT 3 cut(s) 477, 705, 1519
Bst6I CTCTTC 6 cut(s) 42, 313, 498, 540, 565, 1176
BstAFI CTTAAG 1 cut(s) 614
BstC8I GCNNGC 5 cut(s) 237, 283, 456, 462, 894
BstF5I GGATG 5 cut(s) 89, 415, 548, 1081, 1605
BstFNI CGCG 1 cut(s) 593
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 4 cut(s) 558, 1392, 1597, 1650
BstMAI GTCTC 2 cut(s) 567, 1469
BstMBI GATC 4 cut(s) 555, 1389, 1594, 1647
BstMCI CGRYCG 2 cut(s) 129, 139
BstMWI GCNNNNNNNGC 5 cut(s) 85, 241, 488, 893, 1063
BstNI CCWGG 1 cut(s) 1373
BstSCI CCNGG 3 cut(s) 92, 93, 1371
BstSFI CTRYAG 2 cut(s) 882, 1245
BstSLI GKGCMC 1 cut(s) 1555
BstUI CGCG 1 cut(s) 593
BstV1I GCAGC 2 cut(s) 1231, 1246
BstV2I GAAGAC 2 cut(s) 999, 1750
BsuRI GGCC 4 cut(s) 146, 821, 1325, 1436
BtsCI GGATG 5 cut(s) 89, 415, 548, 1081, 1605
BtsI GCAGTG 2 cut(s) 693, 1576
BtsIMutI CAGTG 7 cut(s) 204, 693, 876, 1099, 1339, 1453, 1576
Cac8I GCNNGC 5 cut(s) 237, 283, 456, 462, 894
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 820, 1323
Cfr9I CCCGGG 1 cut(s) 93
CseI GACGC 1 cut(s) 748
CsiI ACCWGGT 1 cut(s) 1371
Csp6I GTAC 2 cut(s) 262, 1423
CviAII CATG 3 cut(s) 380, 553, 1432
CviQI GTAC 2 cut(s) 262, 1423
DpnI GATC 4 cut(s) 557, 1391, 1596, 1649
DpnII GATC 4 cut(s) 555, 1389, 1594, 1647
DraIII CACNNNGTG 1 cut(s) 1375
Eam1104I CTCTTC 6 cut(s) 42, 313, 498, 540, 565, 1176
EarI CTCTTC 6 cut(s) 42, 313, 498, 540, 565, 1176
Eco147I AGGCCT 1 cut(s) 146
Eco24I GRGCYC 2 cut(s) 297, 308
Eco31I GGTCTC 1 cut(s) 1469
Eco57I CTGAAG 2 cut(s) 533, 852
Eco88I CYCGRG 2 cut(s) 93, 1019
EcoO109I RGGNCCY 2 cut(s) 820, 1323
EcoRII CCWGG 1 cut(s) 1371
EcoT22I ATGCAT 1 cut(s) 1582
EcoT38I GRGCYC 2 cut(s) 297, 308
FaeI CATG 3 cut(s) 383, 556, 1435
FalI AAGNNNNNCTT 2 cut(s) 839, 871
FatI CATG 3 cut(s) 379, 552, 1431
FauI CCCGC 1 cut(s) 1120
FauNDI CATATG 2 cut(s) 1386, 1582
FbaI TGATCA 2 cut(s) 555, 1389
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 3 cut(s) 1058, 1245, 1260
FokI GGATG 5 cut(s) 76, 422, 535, 1088, 1612
FriOI GRGCYC 2 cut(s) 297, 308
Fsp4HI GCNGC 3 cut(s) 1058, 1245, 1260
FspBI CTAG 6 cut(s) 104, 282, 1109, 1226, 1674, 1745
GlaI GCGC 1 cut(s) 459
GluI GCNGC 3 cut(s) 1058, 1245, 1260
GsaI CCCAGC 1 cut(s) 188
GsuI CTGGAG 2 cut(s) 7, 271
HaeIII GGCC 4 cut(s) 146, 821, 1325, 1436
HapII CCGG 2 cut(s) 94, 129
HgaI GACGC 1 cut(s) 748
HhaI GCGC 1 cut(s) 460
Hin1II CATG 3 cut(s) 383, 556, 1435
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HinfI GANTC 4 cut(s) 587, 859, 1562, 1670
HpaII CCGG 2 cut(s) 94, 129
HphI GGTGA 2 cut(s) 980, 1361
Hpy166II GTNNAC 4 cut(s) 367, 1425, 1451, 1553
Hpy188III TCNNGA 7 cut(s) 24, 250, 833, 1021, 1340, 1487, 1674
Hpy8I GTNNAC 4 cut(s) 367, 1425, 1451, 1553
HpyAV CCTTC 4 cut(s) 508, 619, 740, 844
HpyCH4III ACNGT 3 cut(s) 477, 705, 1519
HpyCH4IV ACGT 1 cut(s) 1036
HpyCH4V TGCA 8 cut(s) 244, 686, 910, 1220, 1247, 1535, 1553, 1580
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 241, 488, 893, 1063
HpySE526I ACGT 1 cut(s) 1036
Hsp92II CATG 3 cut(s) 383, 556, 1435
HspAI GCGC 1 cut(s) 458
Ksp22I TGATCA 2 cut(s) 555, 1389
Kzo9I GATC 4 cut(s) 555, 1389, 1594, 1647
LguI GCTCTTC 1 cut(s) 313
LmnI GCTCC 1 cut(s) 923
Lsp1109I GCAGC 2 cut(s) 1231, 1246
LweI GCATC 4 cut(s) 473, 1044, 1098, 1567
MabI ACCWGGT 1 cut(s) 1371
MaeI CTAG 6 cut(s) 104, 282, 1109, 1226, 1674, 1745
MaeII ACGT 1 cut(s) 1036
MaeIII GTNAC 4 cut(s) 60, 871, 1522, 1630
MalI GATC 4 cut(s) 557, 1391, 1596, 1649
MboI GATC 4 cut(s) 555, 1389, 1594, 1647
MfeI CAATTG 1 cut(s) 286
MhlI GDGCHC 4 cut(s) 297, 308, 1339, 1555
MmeI TCCRAC 3 cut(s) 79, 901, 1675
Mph1103I ATGCAT 1 cut(s) 1582
MroXI GAANNNNTTC 2 cut(s) 507, 1561
MseI TTAA 5 cut(s) 615, 669, 1085, 1194, 1464
MslI CAYNNNNRTG 2 cut(s) 1094, 1430
MspA1I CMGCKG 1 cut(s) 235
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 2 cut(s) 94, 129
MspR9I CCNGG 3 cut(s) 94, 95, 1373
MunI CAATTG 1 cut(s) 286
Mva1269I GAATGC 2 cut(s) 912, 1222
MvaI CCWGG 1 cut(s) 1373
MvnI CGCG 1 cut(s) 593
MwoI GCNNNNNNNGC 5 cut(s) 85, 241, 488, 893, 1063
NciI CCSGG 2 cut(s) 94, 95
NdeI CATATG 2 cut(s) 1386, 1582
NdeII GATC 4 cut(s) 555, 1389, 1594, 1647
NheI GCTAGC 1 cut(s) 281
NlaIII CATG 3 cut(s) 383, 556, 1435
NlaIV GGNNCC 4 cut(s) 133, 756, 925, 1324
NmeAIII GCCGAG 2 cut(s) 100, 105
NmuCI GTSAC 3 cut(s) 60, 871, 1522
NsiI ATGCAT 1 cut(s) 1582
PaeR7I CTCGAG 1 cut(s) 1019
PceI AGGCCT 1 cut(s) 146
PciSI GCTCTTC 1 cut(s) 313
PctI GAATGC 2 cut(s) 912, 1222
PdmI GAANNNNTTC 2 cut(s) 507, 1561
PfeI GAWTC 4 cut(s) 587, 859, 1562, 1670
PkrI GCNGC 3 cut(s) 1059, 1246, 1261
PsiI TTATAA 1 cut(s) 767
Psp6I CCWGG 1 cut(s) 1371
PspFI CCCAGC 1 cut(s) 184
PspGI CCWGG 1 cut(s) 1371
PspN4I GGNNCC 4 cut(s) 133, 756, 925, 1324
PspPI GGNCC 2 cut(s) 820, 1323
PstI CTGCAG 1 cut(s) 1249
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 2 cut(s) 263, 1424
RsaNI GTAC 2 cut(s) 262, 1423
RseI CAYNNNNRTG 2 cut(s) 1094, 1430
SapI GCTCTTC 1 cut(s) 313
SaqAI TTAA 5 cut(s) 615, 669, 1085, 1194, 1464
SatI GCNGC 3 cut(s) 1058, 1245, 1260
Sau3AI GATC 4 cut(s) 555, 1389, 1594, 1647
Sau96I GGNCC 2 cut(s) 820, 1323
ScrFI CCNGG 3 cut(s) 94, 95, 1373
SduI GDGCHC 4 cut(s) 297, 308, 1339, 1555
SexAI ACCWGGT 1 cut(s) 1371
SfaNI GCATC 4 cut(s) 473, 1044, 1098, 1567
SfcI CTRYAG 2 cut(s) 882, 1245
Sfr274I CTCGAG 1 cut(s) 1019
SlaI CTCGAG 1 cut(s) 1019
SmaI CCCGGG 1 cut(s) 95
SmiMI CAYNNNNRTG 2 cut(s) 1094, 1430
SmlI CTYRAG 3 cut(s) 614, 833, 1019
SmoI CTYRAG 3 cut(s) 614, 833, 1019
SpeI ACTAGT 1 cut(s) 103
SseBI AGGCCT 1 cut(s) 146
SsiI CCGC 5 cut(s) 121, 591, 1057, 1060, 1127
SspI AATATT 1 cut(s) 1615
SspMI CTAG 6 cut(s) 104, 282, 1109, 1226, 1674, 1745
StuI AGGCCT 1 cut(s) 146
StyD4I CCNGG 3 cut(s) 92, 93, 1371
TaaI ACNGT 3 cut(s) 477, 705, 1519
TaiI ACGT 1 cut(s) 1039
TaqI TCGA 3 cut(s) 467, 1020, 1650
TaqII GACCGA 1 cut(s) 153
TauI GCSGC 1 cut(s) 1060
TfiI GAWTC 4 cut(s) 587, 859, 1562, 1670
Tru1I TTAA 5 cut(s) 615, 669, 1085, 1194, 1464
Tru9I TTAA 5 cut(s) 615, 669, 1085, 1194, 1464
TscAI CASTG 7 cut(s) 211, 693, 876, 1099, 1339, 1453, 1576
TseFI GTSAC 3 cut(s) 60, 871, 1522
TseI GCWGC 2 cut(s) 1244, 1259
Tsp45I GTSAC 3 cut(s) 60, 871, 1522
TspDTI ATGAA 4 cut(s) 1020, 1488, 1616, 1653
TspMI CCCGGG 1 cut(s) 93
TspRI CASTG 7 cut(s) 211, 693, 876, 1099, 1339, 1453, 1576
Vha464I CTTAAG 1 cut(s) 614
VneI GTGCAC 1 cut(s) 1551
XapI RAATTY 4 cut(s) 655, 1007, 1143, 1460
XbaI TCTAGA 1 cut(s) 1673
XhoI CTCGAG 1 cut(s) 1019
XmaI CCCGGG 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 366
XmnI GAANNNNTTC 2 cut(s) 507, 1561
XspI CTAG 6 cut(s) 104, 282, 1109, 1226, 1674, 1745
Zsp2I ATGCAT 1 cut(s) 1582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.