Rh1DG091600

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014030
Physical Location & Seq
Forward (+)
17277648 .. 17278073
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG091600.1

Sequence Viewer

Length: 426 bp
ATGCTCGCCTCTTACGCCTGCGTCAAAGTCAAAGCCCACGGCGGTTTTTACAACGACGACTACGACGAGTCCAACTCCCACCGCCGCTCCCCCACCACCACAGGCATCTATTCAGCCGAGCCAATGACGGTCGTGCTGGGCCTCGATGGGCCTACCATAGACTCTTACCCGAAGATAGTTCTCGGGGAAAGCCGGCGGCTGCCTAAGCCGAACGACGGCGTTTGCTCCATCTGCTTGTCGGAGTACCAGGCCAAGGATCCCGTACGGTGCATCCCGGATTGCCATCACTGTTTCCACGTTGATTGCATAGACGAATGGCTGCGCATGAGTGCCACGTGTCCCGTCTGTAGAAACTCTCCGGCTCCGTCGCCGGGCCCGAGTCCTCTGTCCGATTTGGTACCCCTGGCACTCCATTATTCTACGTGA

Protein Analysis

141

Amino Acids

15.43

Weight (kDa)

5.79

Isoelectric Point (pI)

67.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 73 - 117 5.6e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 75 - 117 2.1e-13 Ring finger domain
zf-RING_11 PF17123 75 - 103 1.8e-07 RING-like zinc finger
zf-RING_UBOX PF13445 75 - 114 3.9e-06 RING-type zinc-finger
zf-C3HC4_2 PF13923 75 - 116 6.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014030)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07040
fragaria_vesca FvH4_7g04010
malus_domestica MD00G1152800.v1.1 MD03G1294900.v1.1
prunus_persica Prupe.2G044600_v2.0.a1
pyrus_communis pycom02g23630
rosa_chinensis RchiOBHm_Chr1g0327791
rosa_laevigata RLG00000029964
rosa_multiflora Rmu_sc0005527.1_g000012
rosa_rugosa Rorug01G0071000
rosa_samantha Rh1AG087100 Rh1BG070300 Rh1CG085200 Rh1DG091600 Rh6BG243000 Rh6CG245000
rosa_wichuraiana Rw1G006800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 323
Acc65I GGTACC 1 cut(s) 397
AccB1I GGYRCC 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 87
AciI CCGC 4 cut(s) 42, 82, 85, 196
AclWI GGATC 2 cut(s) 251, 264
AcvI CACGTG 1 cut(s) 336
AfaI GTAC 3 cut(s) 245, 264, 399
AfiI CCNNNNNNNGG 3 cut(s) 215, 253, 371
AflIII ACRYGT 1 cut(s) 335
AjnI CCWGG 2 cut(s) 246, 402
AlwI GGATC 2 cut(s) 251, 264
Ama87I CYCGRG 2 cut(s) 182, 376
AoxI GGCC 4 cut(s) 139, 149, 249, 373
ApaI GGGCCC 1 cut(s) 377
ApeKI GCWGC 2 cut(s) 199, 319
Asp718I GGTACC 1 cut(s) 397
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 4 cut(s) 139, 149, 373, 374
AsuC2I CCSGG 2 cut(s) 275, 372
AvaI CYCGRG 2 cut(s) 182, 376
BaeGI GKGCMC 1 cut(s) 377
BamHI GGATCC 1 cut(s) 256
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 377
BbrPI CACGTG 1 cut(s) 336
BbvI GCAGC 2 cut(s) 186, 306
BccI CCATC 3 cut(s) 140, 236, 291
BceAI ACGGC 2 cut(s) 55, 232
BciT130I CCWGG 2 cut(s) 248, 404
BcnI CCSGG 2 cut(s) 275, 372
BfmI CTRYAG 1 cut(s) 346
BisI GCNGC 4 cut(s) 85, 197, 200, 320
BlsI GCNGC 4 cut(s) 86, 198, 201, 321
Bme1390I CCNGG 4 cut(s) 248, 275, 372, 404
BmeT110I CYCGRG 2 cut(s) 182, 376
BmgT120I GGNCC 4 cut(s) 139, 149, 373, 374
BmiI GGNNCC 4 cut(s) 258, 363, 375, 399
BmrFI CCNGG 4 cut(s) 248, 275, 372, 404
BmsI GCATC 2 cut(s) 114, 279
BplI GAGNNNNNCTC 2 cut(s) 59, 91
Bpu10I CCTNAGC 1 cut(s) 204
BpuMI CCSGG 2 cut(s) 275, 372
BsaAI YACGTR 2 cut(s) 336, 423
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 37, 252, 402
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 253, 371
Bse118I RCCGGY 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 2 cut(s) 248, 404
BseDI CCNNGG 3 cut(s) 37, 252, 402
BseGI GGATG 1 cut(s) 270
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 3 cut(s) 215, 253, 371
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 2 cut(s) 186, 306
BseYI CCCAGC 1 cut(s) 136
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 141, 151, 251, 375
BshNI GGYRCC 1 cut(s) 397
BsiEI CGRYCG 1 cut(s) 132
BsiHKCI CYCGRG 2 cut(s) 182, 376
BsiSI CCGG 4 cut(s) 193, 275, 359, 371
BsiWI CGTACG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 3 cut(s) 215, 253, 371
BsmFI GGGAC 1 cut(s) 324
BsnI GGCC 4 cut(s) 141, 151, 251, 375
BsoBI CYCGRG 2 cut(s) 182, 376
Bsp120I GGGCCC 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 377
Bsp143I GATC 1 cut(s) 256
BspACI CCGC 4 cut(s) 42, 82, 85, 196
BspANI GGCC 4 cut(s) 141, 151, 251, 375
BspLI GGNNCC 4 cut(s) 258, 363, 375, 399
BspPI GGATC 2 cut(s) 251, 264
BspT107I GGYRCC 1 cut(s) 397
BsrBI CCGCTC 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 3 cut(s) 37, 252, 402
BssMI GATC 1 cut(s) 256
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 2 cut(s) 248, 404
Bst4CI ACNGT 3 cut(s) 130, 267, 290
BstBAI YACGTR 2 cut(s) 336, 423
BstC8I GCNNGC 3 cut(s) 6, 19, 194
BstDEI CTNAG 1 cut(s) 204
BstDSI CCRYGG 1 cut(s) 37
BstF5I GGATG 1 cut(s) 270
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 1 cut(s) 259
BstMBI GATC 1 cut(s) 256
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 3 cut(s) 14, 205, 231
BstNI CCWGG 2 cut(s) 248, 404
BstSCI CCNGG 4 cut(s) 246, 273, 370, 402
BstSFI CTRYAG 1 cut(s) 346
BstSLI GKGCMC 1 cut(s) 377
BstV1I GCAGC 2 cut(s) 186, 306
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 4 cut(s) 141, 151, 251, 375
BtgI CCRYGG 1 cut(s) 37
BtsCI GGATG 1 cut(s) 270
BtsIMutI CAGTG 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 6, 19, 194
CfoI GCGC 1 cut(s) 324
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 4 cut(s) 139, 149, 373, 374
CseI GACGC 1 cut(s) 10
Csp6I GTAC 3 cut(s) 244, 263, 398
CspCI CAANNNNNGTGG 2 cut(s) 284, 319
CviAII CATG 1 cut(s) 325
CviQI GTAC 3 cut(s) 244, 263, 398
DdeI CTNAG 1 cut(s) 204
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 377
Eco72I CACGTG 1 cut(s) 336
Eco88I CYCGRG 2 cut(s) 182, 376
EcoRII CCWGG 2 cut(s) 246, 402
EcoT14I CCWWGG 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 1 cut(s) 328
FaiI YATR 3 cut(s) 158, 308, 326
FaqI GGGAC 1 cut(s) 324
FatI CATG 1 cut(s) 324
Fnu4HI GCNGC 4 cut(s) 85, 197, 200, 320
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 4 cut(s) 85, 197, 200, 320
FspI TGCGCA 1 cut(s) 323
GlaI GCGC 1 cut(s) 323
GluI GCNGC 4 cut(s) 85, 197, 200, 320
GsaI CCCAGC 1 cut(s) 140
HaeIII GGCC 4 cut(s) 141, 151, 251, 375
HapII CCGG 4 cut(s) 193, 275, 359, 371
HgaI GACGC 1 cut(s) 10
HhaI GCGC 1 cut(s) 324
Hin1II CATG 1 cut(s) 328
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HinfI GANTC 3 cut(s) 68, 161, 379
HpaII CCGG 4 cut(s) 193, 275, 359, 371
Hpy188I TCNGA 2 cut(s) 241, 391
Hpy99I CGWCG 4 cut(s) 59, 68, 218, 370
HpyCH4III ACNGT 3 cut(s) 130, 267, 290
HpyCH4IV ACGT 3 cut(s) 297, 335, 422
HpyCH4V TGCA 2 cut(s) 270, 306
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 205, 231
HpyF3I CTNAG 1 cut(s) 204
HpySE526I ACGT 3 cut(s) 297, 335, 422
Hsp92II CATG 1 cut(s) 328
HspAI GCGC 1 cut(s) 322
KpnI GGTACC 1 cut(s) 401
KroI GCCGGC 1 cut(s) 192
KroNI GCCGGC 1 cut(s) 194
Kzo9I GATC 1 cut(s) 256
LmnI GCTCC 3 cut(s) 92, 230, 367
Lsp1109I GCAGC 2 cut(s) 186, 306
LweI GCATC 2 cut(s) 114, 279
MaeII ACGT 3 cut(s) 297, 335, 422
MalI GATC 1 cut(s) 258
MbiI CCGCTC 1 cut(s) 87
MboI GATC 1 cut(s) 256
MboII GAAGA 1 cut(s) 184
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 377
MlyI GAGTC 3 cut(s) 77, 155, 388
MmeI TCCRAC 2 cut(s) 96, 219
MnlI CCTC 3 cut(s) 19, 152, 393
MroNI GCCGGC 1 cut(s) 192
MspI CCGG 4 cut(s) 193, 275, 359, 371
MspR9I CCNGG 4 cut(s) 248, 275, 372, 404
MvaI CCWGG 2 cut(s) 248, 404
MwoI GCNNNNNNNGC 3 cut(s) 14, 205, 231
NaeI GCCGGC 1 cut(s) 194
NciI CCSGG 2 cut(s) 275, 372
NdeII GATC 1 cut(s) 256
NgoMIV GCCGGC 1 cut(s) 192
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 4 cut(s) 258, 363, 375, 399
NmeAIII GCCGAG 1 cut(s) 142
NsbI TGCGCA 1 cut(s) 323
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 63, 374
PdiI GCCGGC 1 cut(s) 194
Pfl23II CGTACG 1 cut(s) 262
PfoI TCCNGGA 1 cut(s) 273
PkrI GCNGC 4 cut(s) 86, 198, 201, 321
PleI GAGTC 3 cut(s) 76, 155, 387
PmaCI CACGTG 1 cut(s) 336
PmlI CACGTG 1 cut(s) 336
PpsI GAGTC 3 cut(s) 76, 155, 387
Ppu21I YACGTR 2 cut(s) 336, 423
Psp6I CCWGG 2 cut(s) 246, 402
PspCI CACGTG 1 cut(s) 336
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 2 cut(s) 246, 402
PspLI CGTACG 1 cut(s) 262
PspN4I GGNNCC 4 cut(s) 258, 363, 375, 399
PspOMI GGGCCC 1 cut(s) 373
PspPI GGNCC 4 cut(s) 139, 149, 373, 374
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 3 cut(s) 245, 264, 399
RsaNI GTAC 3 cut(s) 244, 263, 398
SatI GCNGC 4 cut(s) 85, 197, 200, 320
Sau3AI GATC 1 cut(s) 256
Sau96I GGNCC 4 cut(s) 139, 149, 373, 374
SchI GAGTC 3 cut(s) 77, 155, 388
ScrFI CCNGG 4 cut(s) 248, 275, 372, 404
SduI GDGCHC 1 cut(s) 377
SetI ASST 3 cut(s) 300, 338, 425
SfaNI GCATC 2 cut(s) 114, 279
SfcI CTRYAG 1 cut(s) 346
SsiI CCGC 4 cut(s) 42, 82, 85, 196
StyD4I CCNGG 4 cut(s) 246, 273, 370, 402
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 3 cut(s) 130, 267, 290
TaiI ACGT 3 cut(s) 300, 338, 425
TaqI TCGA 1 cut(s) 144
TauI GCSGC 2 cut(s) 87, 199
TscAI CASTG 1 cut(s) 293
TseI GCWGC 2 cut(s) 199, 319
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.