Rh2AG206200

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014837
Physical Location & Seq
Forward (+)
20916532 .. 20917827
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG206200.1

Sequence Viewer

Length: 306 bp
ATGGAAGATCCGGGCCAGTCCAAGACAGAAACAAATACAAAAGATATCGGTGGAACTTGGAAACAATCAATCAAATCATTCAAGGTAGCTATCATGGATGGTATAACTATGGGATGTGGGGCTGGAATTTCACTCCTAGGAATGTTTCGTGTGGTGACTGATAAAACTGTCTTTTGCAATACAGAGGCTCAAATGGATCCTGATGCAGGAGCTTCATATTATCTTTCTCGTCTACCTAGCTACTTAGGAGAATACTTAGCTCTTACAGGGGACAAGCTTAATGGTGCAGGAGAATACTTAGCCTGA

Protein Analysis

101

Amino Acids

10.81

Weight (kDa)

4.73

Isoelectric Point (pI)

14.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 27 - 96 2e-24 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 232
AclWI GGATC 3 cut(s) 2, 191, 204
AcsI RAATTY 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 1 cut(s) 82
AluBI AGCT 5 cut(s) 89, 212, 240, 260, 277
AluI AGCT 5 cut(s) 89, 212, 240, 260, 277
AlwI GGATC 3 cut(s) 2, 191, 204
AoxI GGCC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 126
AspA2I CCTAGG 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 13
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 166
AvrII CCTAGG 1 cut(s) 136
BamHI GGATCC 1 cut(s) 196
BccI CCATC 1 cut(s) 92
BcnI CCSGG 1 cut(s) 12
BfaI CTAG 2 cut(s) 137, 237
BlnI CCTAGG 1 cut(s) 136
Bme1390I CCNGG 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 1 cut(s) 193
BpuMI CCSGG 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 16
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 2 cut(s) 103, 119
BseLI CCNNNNNNNGG 1 cut(s) 206
BseNI ACTGG 1 cut(s) 16
BsgI GTGCAG 1 cut(s) 306
BshFI GGCC 1 cut(s) 15
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 1 cut(s) 15
Bsp143I GATC 2 cut(s) 7, 196
BspANI GGCC 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 198
BspPI GGATC 3 cut(s) 2, 191, 204
BsrI ACTGG 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 2 cut(s) 7, 196
BssT1I CCWWGG 1 cut(s) 136
Bst4CI ACNGT 1 cut(s) 169
BstDEI CTNAG 3 cut(s) 244, 256, 298
BstENI CCTNNNNNAGG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 103, 119
BstKTI GATC 2 cut(s) 10, 199
BstMBI GATC 2 cut(s) 7, 196
BstSCI CCNGG 1 cut(s) 10
BstX2I RGATCY 2 cut(s) 7, 196
BstYI RGATCY 2 cut(s) 7, 196
BsuRI GGCC 1 cut(s) 15
BtsCI GGATG 2 cut(s) 103, 119
Cfr13I GGNCC 1 cut(s) 13
CviAII CATG 1 cut(s) 94
CviJI RGCY 9 cut(s) 15, 89, 122, 188, 212, 240, 260, 277, 302
CviKI_1 RGCY 9 cut(s) 15, 89, 122, 188, 212, 240, 260, 277, 302
DdeI CTNAG 3 cut(s) 244, 256, 298
DpnI GATC 2 cut(s) 9, 198
DpnII GATC 2 cut(s) 7, 196
Eco130I CCWWGG 1 cut(s) 136
Eco32I GATATC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 204
EcoRV GATATC 1 cut(s) 46
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 1 cut(s) 97
FaiI YATR 4 cut(s) 95, 104, 110, 217
FaqI GGGAC 1 cut(s) 284
FatI CATG 1 cut(s) 93
FblI GTMKAC 1 cut(s) 232
FokI GGATG 2 cut(s) 110, 126
FspBI CTAG 2 cut(s) 137, 237
HaeIII GGCC 1 cut(s) 15
HapII CCGG 1 cut(s) 11
Hin1II CATG 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 275
HpaII CCGG 1 cut(s) 11
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 1 cut(s) 233
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 1 cut(s) 233
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4V TGCA 3 cut(s) 177, 206, 287
HpyF3I CTNAG 3 cut(s) 244, 256, 298
Hsp92II CATG 1 cut(s) 97
Kzo9I GATC 2 cut(s) 7, 196
LmnI GCTCC 1 cut(s) 209
LpnPI CCDG 7 cut(s) 24, 29, 108, 192, 213, 252, 273
LweI GCATC 1 cut(s) 193
MaeI CTAG 2 cut(s) 137, 237
MaeIII GTNAC 1 cut(s) 154
MalI GATC 2 cut(s) 9, 198
MboI GATC 2 cut(s) 7, 196
MboII GAAGA 1 cut(s) 17
MflI RGATCY 2 cut(s) 7, 196
MluCI AATT 1 cut(s) 126
MnlI CCTC 1 cut(s) 178
MseI TTAA 1 cut(s) 279
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 1 cut(s) 12
NciI CCSGG 1 cut(s) 12
NdeII GATC 2 cut(s) 7, 196
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 13
PsuI RGATCY 2 cut(s) 7, 196
SaqAI TTAA 1 cut(s) 279
Sau3AI GATC 2 cut(s) 7, 196
Sau96I GGNCC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 12
SetI ASST 7 cut(s) 87, 91, 214, 238, 242, 262, 279
SfaNI GCATC 1 cut(s) 193
Sse9I AATT 1 cut(s) 126
SspMI CTAG 2 cut(s) 137, 237
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 169
TasI AATT 1 cut(s) 126
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TseFI GTSAC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 1 cut(s) 204
XagI CCTNNNNNAGG 1 cut(s) 204
XapI RAATTY 1 cut(s) 126
XmaJI CCTAGG 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 232
XspI CTAG 2 cut(s) 137, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.