Rh2CG146200

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009643
Physical Location & Seq
Reverse (-)
12827117 .. 12828900
1784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG146200.1

Sequence Viewer

Length: 1299 bp
ATGACTCTAGTGATCAACCTATACTCTGCCTCTCGGATGACTGAAAACCAAGTCTATGACGCCGCCGAGGTCTATCTTGCAACCAAGGTCAAACCCTCCGACAAGCTCCTCCTAGTGAGCAAAACCCATGACCAGAAGACCATCAGTTTCGCCAATGCGAAAGCTGCCACCATCATCGACACATTTGAGGACATTGAGCTCAAGTGGAGCCATATCTGCCCCGTTAAGAGATCAGATGCTGTTCAAAACGACAATGAGCCACAAAAACAGTGCTTTATGTTGAGGTTTGGCACCAAACACAAAGAAAAAGTGATGGAGTGTTACTTGCCGTATGTGCTTGCTAAAGCCAAGGACATTAAGCAAGAGGAGAAGTTGTTGAAGATTTTCAGCTGCGGCGGCAATAGCTCTCGTTCGATAGGCCTTGAACACCCTGCCACTTTTGAGACAATGGCGATGGATCCGGAGCAGAAGAAAATGGTTATGGATGATTTGGATCGGTTTGTGAGGAGGAGGGACTTTTACAAGAGGGTTGGCAAGGCTTGGAAGAGAGGGTATTTGTTGTACGGTCCTCCTGGAACTGGCAAATCAAGCTTGATTGCAGCCATGGCTAATCATCTCAAGTTTGACATATATGATTTGGAGCTTTCTAGTATTTCTAGGAACTCGGACTTGAGGAGTGTGCTTCTATCTACTTCAAATCGTTCTATTCTAGTAATTGAGGACATTGATTGCAGTGTGGAAGCCGAAAATCGGGTGGTAGCTGAGAAGTTCATGGGGAGTAGATACAATAGACCATCACAAATGAAGTTTACACTCTCAGGTCTACTTAATGTCATTGATGGTCTGTGGTCATGTGTTGGAGATGAGAGGATCATCATCTTCACCACTAACCACAAGGATCGGCTAGACCCGGCATTGTTGCGACCTGGACGAATGGATGTGCACATTCACATGTCCTATTGTACTCCAAGCGGGTTCAGGATCTTGGCCTCTAACTACCTTGGCATTCATGAGAGCAACCCTCATCGGCTCTGTGGAGAAATTGAAGGGTTGATAGAGAGCACAGAGGTCACCCCTGCACAGGTTGCTGAGGAGCTTATGCAGAGTGATGATGCTGATGTTGCTCTTGAAGGGCTTGTCAATTTTCTCAAATGCAAGATACAGGTTGATAGCAAGAAAACAAAGGATGAAGGGACTAAGAAAGCAGAGAGTTTCGAAGAAGAGTTGGAACAACTTGATAATGGTACTCACCCAATTTTTTCCAAGACTAGTGCTGCGTTTTACAAAGATGTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

432

Amino Acids

48.86

Weight (kDa)

6.3

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 1 - 73 5.1e-13 Domain associated at C-terminal with AAA
AAA PF00004 185 - 319 5.2e-20 ATPase family associated with various cellular activities (AAA)
RuvB_N PF05496 185 - 243 5.2e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA_lid_At3g28540 PF25568 321 - 393 2e-21 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 290
AccI GTMKAC 1 cut(s) 823
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 4 cut(s) 63, 393, 396, 972
AclWI GGATC 6 cut(s) 452, 465, 501, 878, 906, 989
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 3 cut(s) 563, 964, 1246
AfiI CCNNNNNNNGG 3 cut(s) 578, 750, 1081
AflIII ACRYGT 1 cut(s) 951
AgsI TTSAA 6 cut(s) 245, 379, 425, 696, 1046, 1130
AhlI ACTAGT 1 cut(s) 1268
AjnI CCWGG 2 cut(s) 571, 925
AluBI AGCT 9 cut(s) 106, 164, 199, 390, 405, 591, 643, 761, 1096
AluI AGCT 9 cut(s) 106, 164, 199, 390, 405, 591, 643, 761, 1096
Alw21I GWGCWC 3 cut(s) 201, 945, 1064
Alw26I GTCTC 1 cut(s) 437
Alw44I GTGCAC 1 cut(s) 941
AlwI GGATC 6 cut(s) 452, 465, 501, 878, 906, 989
AlwNI CAGNNNCTG 1 cut(s) 239
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 2 cut(s) 418, 987
ApaLI GTGCAC 1 cut(s) 941
ApeKI GCWGC 4 cut(s) 164, 390, 599, 1274
Asp700I GAANNNNTTC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 566
AsuC2I CCSGG 1 cut(s) 911
AsuHPI GGTGA 3 cut(s) 874, 1063, 1241
AsuII TTCGAA 1 cut(s) 1215
AvaII GGWCC 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 945
BamHI GGATCC 1 cut(s) 457
BanI GGYRCC 1 cut(s) 290
BanII GRGCYC 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 143
Bbv12I GWGCWC 3 cut(s) 201, 945, 1064
BbvCI CCTCAGC 1 cut(s) 1089
BbvI GCAGC 4 cut(s) 151, 377, 611, 1261
BccI CCATC 6 cut(s) 149, 179, 307, 448, 802, 833
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 2 cut(s) 573, 927
BclI TGATCA 1 cut(s) 12
BcnI CCSGG 1 cut(s) 911
BcoDI GTCTC 1 cut(s) 437
BcuI ACTAGT 1 cut(s) 1268
BfaI CTAG 7 cut(s) 8, 113, 648, 657, 710, 905, 1269
BisI GCNGC 7 cut(s) 63, 165, 391, 394, 397, 600, 1275
BlsI GCNGC 7 cut(s) 64, 166, 392, 395, 398, 601, 1276
Bme1390I CCNGG 3 cut(s) 573, 911, 927
Bme18I GGWCC 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 3 cut(s) 209, 292, 459
BmrFI CCNGG 3 cut(s) 573, 911, 927
BmsI GCATC 2 cut(s) 226, 1102
BpiI GAAGAC 1 cut(s) 143
BplI GAGNNNNNCTC 2 cut(s) 1006, 1038
Bpu10I CCTNAGC 1 cut(s) 1089
Bpu14I TTCGAA 1 cut(s) 1215
BpuEI CTTGAG 3 cut(s) 185, 602, 691
BpuMI CCSGG 1 cut(s) 911
BsaBI GATNNNNATC 2 cut(s) 492, 875
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 5 cut(s) 66, 84, 348, 603, 1000
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 3 cut(s) 578, 750, 1081
Bse1I ACTGG 1 cut(s) 583
Bse8I GATNNNNATC 2 cut(s) 492, 875
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 2 cut(s) 573, 927
BseDI CCNNGG 5 cut(s) 66, 84, 348, 603, 1000
BseGI GGATG 4 cut(s) 42, 490, 943, 1192
BseJI GATNNNNATC 2 cut(s) 492, 875
BseLI CCNNNNNNNGG 3 cut(s) 578, 750, 1081
BseMII CTCAG 3 cut(s) 753, 831, 1080
BseNI ACTGG 1 cut(s) 583
BseRI GAGGAG 6 cut(s) 98, 380, 520, 523, 688, 1106
BseSI GKGCMC 1 cut(s) 945
BseXI GCAGC 4 cut(s) 151, 377, 611, 1261
BsgI GTGCAG 1 cut(s) 1062
BshFI GGCC 2 cut(s) 420, 989
BshNI GGYRCC 1 cut(s) 290
BsiHKAI GWGCWC 3 cut(s) 201, 945, 1064
BsiSI CCGG 2 cut(s) 461, 911
BslFI GGGAC 2 cut(s) 527, 1207
BslI CCNNNNNNNGG 3 cut(s) 578, 750, 1081
BsmAI GTCTC 1 cut(s) 437
BsmFI GGGAC 2 cut(s) 527, 1207
BsmI GAATGC 1 cut(s) 1005
BsnI GGCC 2 cut(s) 420, 989
Bsp119I TTCGAA 1 cut(s) 1215
Bsp1286I GDGCHC 3 cut(s) 201, 945, 1064
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 7 cut(s) 12, 230, 457, 493, 870, 898, 981
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 4 cut(s) 63, 393, 396, 972
BspANI GGCC 2 cut(s) 420, 989
BspCNI CTCAG 3 cut(s) 754, 830, 1081
BspEI TCCGGA 1 cut(s) 460
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 3 cut(s) 209, 292, 459
BspPI GGATC 6 cut(s) 452, 465, 501, 878, 906, 989
BspT104I TTCGAA 1 cut(s) 1215
BspT107I GGYRCC 1 cut(s) 290
BsrI ACTGG 1 cut(s) 583
BssECI CCNNGG 5 cut(s) 66, 84, 348, 603, 1000
BssMI GATC 7 cut(s) 12, 230, 457, 493, 870, 898, 981
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 4 cut(s) 84, 348, 603, 1000
Bst2UI CCWGG 2 cut(s) 573, 927
Bst4CI ACNGT 2 cut(s) 270, 566
Bst6I CTCTTC 2 cut(s) 539, 1215
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 1085
BstBI TTCGAA 1 cut(s) 1215
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 4 cut(s) 762, 817, 1089, 1197
BstDSI CCRYGG 1 cut(s) 603
BstEII GGTNACC 1 cut(s) 1069
BstF5I GGATG 4 cut(s) 42, 490, 943, 1192
BstKTI GATC 7 cut(s) 15, 233, 460, 496, 873, 901, 984
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 7 cut(s) 12, 230, 457, 493, 870, 898, 981
BstMWI GCNNNNNNNGC 9 cut(s) 164, 216, 334, 396, 402, 588, 605, 1085, 1121
BstNI CCWGG 2 cut(s) 573, 927
BstNSI RCATGY 1 cut(s) 955
BstPI GGTNACC 1 cut(s) 1069
BstSCI CCNGG 3 cut(s) 571, 909, 925
BstSLI GKGCMC 1 cut(s) 945
BstV1I GCAGC 4 cut(s) 151, 377, 611, 1261
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 2 cut(s) 457, 981
BstYI RGATCY 2 cut(s) 457, 981
BsuRI GGCC 2 cut(s) 420, 989
BtgI CCRYGG 1 cut(s) 603
BtgZI GCGATG 1 cut(s) 467
BtsCI GGATG 4 cut(s) 42, 490, 943, 1192
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 2 cut(s) 275, 739
Cac8I GCNNGC 1 cut(s) 339
CaiI CAGNNNCTG 1 cut(s) 239
CciI TCATGA 1 cut(s) 1009
Cfr13I GGNCC 1 cut(s) 566
CseI GACGC 1 cut(s) 68
Csp6I GTAC 3 cut(s) 562, 963, 1245
CviAII CATG 6 cut(s) 128, 604, 772, 852, 952, 1010
CviQI GTAC 3 cut(s) 562, 963, 1245
DdeI CTNAG 4 cut(s) 762, 817, 1089, 1197
DpnI GATC 7 cut(s) 14, 232, 459, 495, 872, 900, 983
DpnII GATC 7 cut(s) 12, 230, 457, 493, 870, 898, 981
Eam1104I CTCTTC 2 cut(s) 539, 1215
EarI CTCTTC 2 cut(s) 539, 1215
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 4 cut(s) 84, 348, 603, 1000
Eco147I AGGCCT 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 566
Eco53kI GAGCTC 1 cut(s) 199
Eco91I GGTNACC 1 cut(s) 1069
EcoICRI GAGCTC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 1069
EcoRII CCWGG 2 cut(s) 571, 925
EcoT14I CCWWGG 4 cut(s) 84, 348, 603, 1000
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 4 cut(s) 84, 348, 603, 1000
FaeI CATG 6 cut(s) 131, 607, 775, 855, 955, 1013
FaqI GGGAC 2 cut(s) 527, 1207
FatI CATG 6 cut(s) 127, 603, 771, 851, 951, 1009
FauI CCCGC 1 cut(s) 965
FbaI TGATCA 1 cut(s) 12
FblI GTMKAC 1 cut(s) 823
Fnu4HI GCNGC 7 cut(s) 63, 165, 391, 394, 397, 600, 1275
FokI GGATG 4 cut(s) 49, 497, 950, 1199
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 7 cut(s) 63, 165, 391, 394, 397, 600, 1275
FspBI CTAG 7 cut(s) 8, 113, 648, 657, 710, 905, 1269
GluI GCNGC 7 cut(s) 63, 165, 391, 394, 397, 600, 1275
HaeIII GGCC 2 cut(s) 420, 989
HapII CCGG 2 cut(s) 461, 911
HgaI GACGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 6 cut(s) 131, 607, 775, 855, 955, 1013
HindIII AAGCTT 1 cut(s) 589
HinfI GANTC 1 cut(s) 4
HpaII CCGG 2 cut(s) 461, 911
HphI GGTGA 3 cut(s) 874, 1063, 1241
Hpy166II GTNNAC 3 cut(s) 810, 824, 943
Hpy188I TCNGA 4 cut(s) 36, 100, 235, 667
Hpy188III TCNNGA 4 cut(s) 461, 979, 1010, 1127
Hpy8I GTNNAC 3 cut(s) 810, 824, 943
HpyAV CCTTC 3 cut(s) 1040, 1124, 1184
HpyCH4III ACNGT 2 cut(s) 270, 566
HpyCH4V TGCA 7 cut(s) 80, 599, 732, 943, 1079, 1102, 1155
HpyF10VI GCNNNNNNNGC 9 cut(s) 164, 216, 334, 396, 402, 588, 605, 1085, 1121
HpyF3I CTNAG 4 cut(s) 762, 817, 1089, 1197
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 6 cut(s) 131, 607, 775, 855, 955, 1013
Kpn2I TCCGGA 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 7 cut(s) 12, 230, 457, 493, 870, 898, 981
LmnI GCTCC 5 cut(s) 111, 207, 463, 640, 1093
Lsp1109I GCAGC 4 cut(s) 151, 377, 611, 1261
LweI GCATC 2 cut(s) 226, 1102
MaeI CTAG 7 cut(s) 8, 113, 648, 657, 710, 905, 1269
MaeIII GTNAC 2 cut(s) 320, 1069
MalI GATC 7 cut(s) 14, 232, 459, 495, 872, 900, 983
MboI GATC 7 cut(s) 12, 230, 457, 493, 870, 898, 981
MboII GAAGA 7 cut(s) 148, 391, 481, 556, 871, 1229, 1232
MflI RGATCY 2 cut(s) 457, 981
MhlI GDGCHC 3 cut(s) 201, 945, 1064
MluCI AATT 4 cut(s) 714, 1041, 1141, 1254
MmeI TCCRAC 3 cut(s) 123, 838, 1206
MroI TCCGGA 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 383
MseI TTAA 3 cut(s) 225, 357, 828
MslI CAYNNNNRTG 1 cut(s) 950
MspA1I CMGCKG 1 cut(s) 390
MspI CCGG 2 cut(s) 461, 911
MspR9I CCNGG 3 cut(s) 573, 911, 927
Mva1269I GAATGC 1 cut(s) 1005
MvaI CCWGG 2 cut(s) 573, 927
MwoI GCNNNNNNNGC 9 cut(s) 164, 216, 334, 396, 402, 588, 605, 1085, 1121
NciI CCSGG 1 cut(s) 911
NcoI CCATGG 1 cut(s) 603
NdeII GATC 7 cut(s) 12, 230, 457, 493, 870, 898, 981
NlaIII CATG 6 cut(s) 131, 607, 775, 855, 955, 1013
NlaIV GGNNCC 3 cut(s) 209, 292, 459
NmeAIII GCCGAG 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 1069
NspI RCATGY 1 cut(s) 955
NspV TTCGAA 1 cut(s) 1215
PagI TCATGA 1 cut(s) 1009
PceI AGGCCT 1 cut(s) 420
PciI ACATGT 1 cut(s) 951
PctI GAATGC 1 cut(s) 1005
PdmI GAANNNNTTC 1 cut(s) 383
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 7 cut(s) 64, 166, 392, 395, 398, 601, 1276
PscI ACATGT 1 cut(s) 951
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 2 cut(s) 571, 925
PspEI GGTNACC 1 cut(s) 1069
PspGI CCWGG 2 cut(s) 571, 925
PspN4I GGNNCC 3 cut(s) 209, 292, 459
PspPI GGNCC 1 cut(s) 566
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 2 cut(s) 457, 981
PvuII CAGCTG 1 cut(s) 390
RsaI GTAC 3 cut(s) 563, 964, 1246
RsaNI GTAC 3 cut(s) 562, 963, 1245
RseI CAYNNNNRTG 1 cut(s) 950
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 3 cut(s) 225, 357, 828
SatI GCNGC 7 cut(s) 63, 165, 391, 394, 397, 600, 1275
Sau3AI GATC 7 cut(s) 12, 230, 457, 493, 870, 898, 981
Sau96I GGNCC 1 cut(s) 566
ScrFI CCNGG 3 cut(s) 573, 911, 927
SduI GDGCHC 3 cut(s) 201, 945, 1064
SfaNI GCATC 2 cut(s) 226, 1102
SfuI TTCGAA 1 cut(s) 1215
SinI GGWCC 1 cut(s) 566
SmiMI CAYNNNNRTG 1 cut(s) 950
SmlI CTYRAG 3 cut(s) 200, 617, 670
SmoI CTYRAG 3 cut(s) 200, 617, 670
SpeI ACTAGT 1 cut(s) 1268
Sse9I AATT 4 cut(s) 714, 1041, 1141, 1254
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 4 cut(s) 63, 393, 396, 972
SspMI CTAG 7 cut(s) 8, 113, 648, 657, 710, 905, 1269
SstI GAGCTC 1 cut(s) 201
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 3 cut(s) 571, 909, 925
StyI CCWWGG 4 cut(s) 84, 348, 603, 1000
TaaI ACNGT 2 cut(s) 270, 566
TaqI TCGA 3 cut(s) 177, 413, 1215
TasI AATT 4 cut(s) 714, 1041, 1141, 1254
TatI WGTACW 1 cut(s) 962
TauI GCSGC 3 cut(s) 65, 396, 399
Tru1I TTAA 3 cut(s) 225, 357, 828
Tru9I TTAA 3 cut(s) 225, 357, 828
TscAI CASTG 2 cut(s) 275, 739
TseFI GTSAC 1 cut(s) 1069
TseI GCWGC 4 cut(s) 164, 390, 599, 1274
Tsp45I GTSAC 1 cut(s) 1069
TspDTI ATGAA 4 cut(s) 760, 818, 998, 1203
TspRI CASTG 2 cut(s) 275, 739
VneI GTGCAC 1 cut(s) 941
VpaK11BI GGWCC 1 cut(s) 566
XceI RCATGY 1 cut(s) 955
XmiI GTMKAC 1 cut(s) 823
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 7 cut(s) 8, 113, 648, 657, 710, 905, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.