Rh3DG309900

respiratory chain complex III assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0009643
Physical Location & Seq
Forward (+)
33276057 .. 33283584
7528 bp
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UTR
Exon/CDS
Intron
Rh3DG309900.1

Sequence Viewer

Length: 438 bp
ATGCACTGTATCTTGATAATCCTTAATTCGACCGAGAGTCCTGGTTTAAAGCTCATAGCAAGGCATTGCTGCGTCCGGGCCGAATGGACATGCACATTAACATGTCCTATTGCACTGCAGGGGGTTTTAACATATTGGCCTCGAACAATCTTGGCATTCATCACATGCAGCAACCGTCACAGGCTTTGTGGTGGAATTGAAGGACTAATTGAGAGTAGTGAGGTCACTCCTGCAGAGGTTGCTGAGGAGCTATTGAGGATCGATAATGCTGATATTGCTCTTGAAGGACTAGTGAGTTTGCTCAAGGGAAAGAAAGTTGAGAGAAATGAAGCAAAGGAGGAGGGGAGTAACAAAGTTGAGATTCAAGATGTAAAGAGGCAGAAAACTGAGTCAAATGTCAATGGTGACAATCAAAACATGTTGCAGCTGAATCAATAG

Protein Analysis

145

Amino Acids

16.11

Weight (kDa)

5.65

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_lid_At3g28540 PF25568 58 - 112 1.2e-11 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 266
AflIII ACRYGT 2 cut(s) 101, 417
AgsI TTSAA 3 cut(s) 200, 284, 365
AhdI GACNNNNNGTC 1 cut(s) 36
AhlI ACTAGT 1 cut(s) 289
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 3 cut(s) 52, 250, 427
AluI AGCT 3 cut(s) 52, 250, 427
AlwI GGATC 1 cut(s) 266
AoxI GGCC 2 cut(s) 78, 137
ApeKI GCWGC 3 cut(s) 69, 168, 424
AspS9I GGNCC 1 cut(s) 78
AsuC2I CCSGG 1 cut(s) 77
AsuHPI GGTGA 1 cut(s) 416
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 56, 180
BciT130I CCWGG 1 cut(s) 42
BcnI CCSGG 1 cut(s) 77
BcuI ACTAGT 1 cut(s) 289
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 2 cut(s) 116, 231
BisI GCNGC 3 cut(s) 70, 169, 425
BlsI GCNGC 3 cut(s) 71, 170, 426
Bme1390I CCNGG 2 cut(s) 42, 77
BmeRI GACNNNNNGTC 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 78
BmrFI CCNGG 2 cut(s) 42, 77
Bpu10I CCTNAGC 1 cut(s) 243
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 77
Bsa29I ATCGAT 1 cut(s) 261
Bse3DI GCAATG 1 cut(s) 64
BseBI CCWGG 1 cut(s) 42
BseCI ATCGAT 1 cut(s) 261
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 2 cut(s) 234, 378
BseRI GAGGAG 2 cut(s) 260, 353
BseXI GCAGC 2 cut(s) 56, 180
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 2 cut(s) 80, 139
BshVI ATCGAT 1 cut(s) 261
BsiEI CGRYCG 1 cut(s) 33
BsiSI CCGG 1 cut(s) 76
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 2 cut(s) 80, 139
Bsp143I GATC 1 cut(s) 258
BspANI GGCC 2 cut(s) 80, 139
BspCNI CTCAG 2 cut(s) 235, 379
BspDI ATCGAT 1 cut(s) 261
BspMAI CTGCAG 2 cut(s) 120, 235
BspPI GGATC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 64
BssMI GATC 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 8, 176
BstAPI GCANNNNNTGC 1 cut(s) 239
BstDEI CTNAG 2 cut(s) 243, 387
BstKTI GATC 1 cut(s) 261
BstMBI GATC 1 cut(s) 258
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 2 cut(s) 239, 275
BstNI CCWGG 1 cut(s) 42
BstNSI RCATGY 4 cut(s) 93, 105, 168, 421
BstSCI CCNGG 2 cut(s) 40, 75
BstSFI CTRYAG 2 cut(s) 116, 231
BstV1I GCAGC 2 cut(s) 56, 180
Bsu15I ATCGAT 1 cut(s) 261
BsuRI GGCC 2 cut(s) 80, 139
BsuTUI ATCGAT 1 cut(s) 261
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 2 cut(s) 4, 113
Cfr13I GGNCC 1 cut(s) 78
ClaI ATCGAT 1 cut(s) 261
CseI GACGC 1 cut(s) 61
CviAII CATG 4 cut(s) 90, 102, 165, 418
CviJI RGCY 6 cut(s) 52, 80, 139, 184, 250, 427
CviKI_1 RGCY 6 cut(s) 52, 80, 139, 184, 250, 427
DdeI CTNAG 2 cut(s) 243, 387
DpnI GATC 1 cut(s) 260
DpnII GATC 1 cut(s) 258
DraI TTTAAA 1 cut(s) 48
DriI GACNNNNNGTC 1 cut(s) 36
Eam1105I GACNNNNNGTC 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 40
FaeI CATG 4 cut(s) 93, 105, 168, 421
FaiI YATR 6 cut(s) 56, 91, 103, 133, 166, 419
FatI CATG 4 cut(s) 89, 101, 164, 417
Fnu4HI GCNGC 3 cut(s) 70, 169, 425
Fsp4HI GCNGC 3 cut(s) 70, 169, 425
FspBI CTAG 1 cut(s) 290
GluI GCNGC 3 cut(s) 70, 169, 425
HaeIII GGCC 2 cut(s) 80, 139
HapII CCGG 1 cut(s) 76
HgaI GACGC 1 cut(s) 61
Hin1II CATG 4 cut(s) 93, 105, 168, 421
HinfI GANTC 4 cut(s) 37, 361, 389, 430
HpaII CCGG 1 cut(s) 76
HphI GGTGA 1 cut(s) 416
Hpy188III TCNNGA 3 cut(s) 13, 281, 365
HpyAV CCTTC 2 cut(s) 194, 278
HpyCH4III ACNGT 2 cut(s) 8, 176
HpyCH4V TGCA 7 cut(s) 4, 93, 113, 118, 168, 233, 424
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 275
HpyF3I CTNAG 2 cut(s) 243, 387
Hsp92II CATG 4 cut(s) 93, 105, 168, 421
Kzo9I GATC 1 cut(s) 258
LmnI GCTCC 1 cut(s) 247
LpnPI CCDG 6 cut(s) 27, 54, 89, 104, 166, 243
Lsp1109I GCAGC 2 cut(s) 56, 180
MaeI CTAG 1 cut(s) 290
MaeIII GTNAC 4 cut(s) 176, 223, 347, 404
MalI GATC 1 cut(s) 260
MboI GATC 1 cut(s) 258
MluCI AATT 3 cut(s) 25, 195, 207
MlyI GAGTC 2 cut(s) 46, 398
MnlI CCTC 8 cut(s) 150, 214, 229, 238, 249, 331, 334, 369
MseI TTAA 4 cut(s) 24, 47, 98, 128
MslI CAYNNNNRTG 1 cut(s) 100
MspA1I CMGCKG 1 cut(s) 427
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 42, 77
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 2 cut(s) 239, 275
NciI CCSGG 1 cut(s) 77
NdeII GATC 1 cut(s) 258
NlaIII CATG 4 cut(s) 93, 105, 168, 421
NmuCI GTSAC 3 cut(s) 176, 223, 404
NspI RCATGY 4 cut(s) 93, 105, 168, 421
PciI ACATGT 2 cut(s) 101, 417
PctI GAATGC 1 cut(s) 155
PfeI GAWTC 2 cut(s) 361, 430
PkrI GCNGC 3 cut(s) 71, 170, 426
PleI GAGTC 2 cut(s) 45, 397
PpsI GAGTC 2 cut(s) 45, 397
PscI ACATGT 2 cut(s) 101, 417
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspPI GGNCC 1 cut(s) 78
PstI CTGCAG 2 cut(s) 120, 235
PvuII CAGCTG 1 cut(s) 427
RseI CAYNNNNRTG 1 cut(s) 100
SaqAI TTAA 4 cut(s) 24, 47, 98, 128
SatI GCNGC 3 cut(s) 70, 169, 425
Sau3AI GATC 1 cut(s) 258
Sau96I GGNCC 1 cut(s) 78
SchI GAGTC 2 cut(s) 46, 398
ScrFI CCNGG 2 cut(s) 42, 77
SetI ASST 5 cut(s) 54, 225, 240, 252, 429
SfcI CTRYAG 2 cut(s) 116, 231
SmiMI CAYNNNNRTG 1 cut(s) 100
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SpeI ACTAGT 1 cut(s) 289
Sse9I AATT 3 cut(s) 25, 195, 207
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 2 cut(s) 40, 75
TaaI ACNGT 2 cut(s) 8, 176
TaqI TCGA 3 cut(s) 29, 142, 261
TaqII GACCGA 1 cut(s) 47
TasI AATT 3 cut(s) 25, 195, 207
TfiI GAWTC 2 cut(s) 361, 430
Tru1I TTAA 4 cut(s) 24, 47, 98, 128
Tru9I TTAA 4 cut(s) 24, 47, 98, 128
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 3 cut(s) 176, 223, 404
TseI GCWGC 3 cut(s) 69, 168, 424
Tsp45I GTSAC 3 cut(s) 176, 223, 404
TspDTI ATGAA 2 cut(s) 148, 342
TspRI CASTG 1 cut(s) 120
XceI RCATGY 4 cut(s) 93, 105, 168, 421
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.