Rh3AG283300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0003395
Physical Location & Seq
Reverse (-)
33084523 .. 33088155
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG283300.1

Sequence Viewer

Length: 1677 bp
ATGCTCGACAGAAGAAGTATATCCACAGCCTTGTCATCGCCATGTCTTGATGATTTGACTACTAAGGGGCTTCACCTTATAGCTACCACACTTGCAGCAGGTTCAGTCAAGTTTGAGAACACTCGTTGCGAGATGAAGAAGTTTATCAGAGAATCTCTGCAAAAAAAATTTAGAAGCCAAAATAATCATGACGATGAGTTGGGCATTTTTAAGCAACTATCTCAACATCTTAGTGACCCACAAAATTTCCGAGATAAGGTTGTTACAGTTCTTTCCAGATCACAGTCTCAACGTCTTGCTGTTAGAAAGGTACTGGATGGACTTGAGGGCTTGCCTTCTGAGACCCTATTTGCAATGAGCAGAAAGCTTAAAGGAGTACAACATTTACCTCAGTTACATCAAAAGAAAAATACATTGTACAAAAAGACTTTGATTGATCAATTGCGGAAAACTTGTGACACGATGCTTTCCGAACTTGGTACAGGAGATGAACTGCAGGAATCACTAGCCAAAGCCATGGCAGTAGCAGGCCTATCAGTGAAGTTACAACCAGGTCTCCATAATTCGACTGTAACAGAATTTCGCCGATTTTCAAAAGAGGTGAAGTTACATCCAGGTCTCCATAATTCGACTGTAACAGAATTTCGCCGATTTTCACAAGAGATGAAGATACTGCAGGATGATATTGCAAGGGCTATTTGGCTAGTTGAAAAAAAGGTTAGCTTGCTAGAGCTCGAAAAGTTGAAGACTATATTAGGCCGAGATGCTGAAGTATCAAACAGGAGCTTACGATATGCAATTAAGAAAATGCTAACTGAATATCTCTTCGAGTGTGGTGATATGGATACAATTCCGAAGTCATTATTGGAAGCTCTTGATATTATCAACATGTCACGAAACAAGCTGCATAGACTCTTCTTAAAGGATGAAATTGAAGAAGAGGTGGAATGTATATTGAATGTGAGTGCTCAGGCAAAGCAGATTGTATGGGATATGTTCCCTGACCATGAGTTAGATCTAGACTTTGCTGAGGCATATGTTGAGGAAATGGAGGATGAAGATGATGATGATGATGACGATGACGAGGATGATGGTGATGATAGGTCTAGCAATGATGATAACAACCATAATCATGGTTCGCCACAGGAACAAGGAATATCTGGAAGTGACAGATCTCACTCTGATGATTCACATTATGAGGATGAAAGCACTGGGGAAACAATGTTCTCCAATCAAGGAACTTCTCATGATTTCAATGGTATTAGACGAGGGTTTCCTGGGACTTCTACTGAGAGACATGAACCTGAACATGACACTGGAATGGATACAGATGACCCTTTGCATGTCGAGCCAGATGATGTGCATAAACAAACCACTTGTAACCAATACCTTGCAATTCAAGAAGTGTCTGATAAGACAAGCATGATAGCCCATGATCTTGTTGGTCATATGTTGGAGGAGTTTGCACAGAAAGATGGCTTGGATTTGGACTGGGATGATACATTATATCTGAAAGGTGATTGTGCAACTCGAGAAGATTCAGGAGTGGTAGAAGAAAGGCAGACATTGTCTCAGGAGAACAGAGGCGGTTCTGTAATTGTTAGTGTAATTGAGGATCTAATACCTTCATTTCCAAAGAGTGGAATCGAAGCATTAAGGAACTTGGTGGGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

558

Amino Acids

63.21

Weight (kDa)

4.89

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 1640
AciI CCGC 2 cut(s) 445, 1587
AclWI GGATC 1 cut(s) 1623
AcsI RAATTY 4 cut(s) 167, 244, 578, 641
AcuI CTGAAG 1 cut(s) 789
AfaI GTAC 4 cut(s) 312, 378, 419, 481
AfiI CCNNNNNNNGG 2 cut(s) 256, 1640
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 7 cut(s) 594, 710, 745, 935, 958, 1255, 1400
AjnI CCWGG 3 cut(s) 550, 613, 1276
AjuI GAANNNNNNNTTGG 4 cut(s) 848, 880, 1463, 1495
AloI GAACNNNNNNTCC 2 cut(s) 1208, 1240
AluBI AGCT 7 cut(s) 83, 367, 723, 733, 786, 872, 904
AluI AGCT 7 cut(s) 83, 367, 723, 733, 786, 872, 904
Alw21I GWGCWC 2 cut(s) 735, 970
Alw26I GTCTC 6 cut(s) 291, 335, 560, 623, 1288, 1575
AlwI GGATC 1 cut(s) 1623
Ama87I CYCGRG 1 cut(s) 1530
AoxI GGCC 2 cut(s) 529, 757
ApeKI GCWGC 2 cut(s) 95, 904
ApoI RAATTY 4 cut(s) 167, 244, 578, 641
AsuHPI GGTGA 5 cut(s) 65, 613, 848, 1106, 1529
AvaI CYCGRG 1 cut(s) 1530
BanII GRGCYC 1 cut(s) 735
BbsI GAAGAC 1 cut(s) 752
Bbv12I GWGCWC 2 cut(s) 735, 970
BbvCI CCTCAGC 1 cut(s) 1029
BbvI GCAGC 2 cut(s) 107, 891
BccI CCATC 3 cut(s) 311, 1085, 1469
BciT130I CCWGG 3 cut(s) 552, 615, 1278
BciVI GTATCC 2 cut(s) 838, 1318
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 6 cut(s) 291, 335, 560, 623, 1288, 1575
BfaI CTAG 5 cut(s) 506, 704, 728, 1019, 1107
BfmI CTRYAG 2 cut(s) 494, 674
BfuAI ACCTGC 1 cut(s) 89
BfuI GTATCC 2 cut(s) 838, 1318
BglII AGATCT 2 cut(s) 1015, 1172
BisI GCNGC 2 cut(s) 96, 905
BlsI GCNGC 2 cut(s) 97, 906
Bme1390I CCNGG 3 cut(s) 552, 615, 1278
BmeT110I CYCGRG 1 cut(s) 1530
BmrFI CCNGG 3 cut(s) 552, 615, 1278
BmrI ACTGGG 2 cut(s) 1221, 1501
BmsI GCATC 2 cut(s) 453, 754
BmuI ACTGGG 2 cut(s) 1221, 1501
BpiI GAAGAC 1 cut(s) 752
Bpu10I CCTNAGC 2 cut(s) 969, 1029
BpuEI CTTGAG 1 cut(s) 344
BsaI GGTCTC 3 cut(s) 335, 560, 623
BsaJI CCNNGG 2 cut(s) 516, 1277
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 1640
Bse1I ACTGG 4 cut(s) 318, 1216, 1321, 1496
Bse3DI GCAATG 2 cut(s) 360, 1117
BseBI CCWGG 3 cut(s) 552, 615, 1278
BseDI CCNNGG 2 cut(s) 516, 1277
BseGI GGATG 8 cut(s) 322, 610, 685, 931, 1060, 1093, 1207, 1501
BseLI CCNNNNNNNGG 2 cut(s) 256, 1640
BseMI GCAATG 2 cut(s) 360, 1117
BseMII CTCAG 6 cut(s) 330, 404, 983, 1020, 1281, 1586
BseNI ACTGG 4 cut(s) 318, 1216, 1321, 1496
BseRI GAGGAG 1 cut(s) 1472
BseXI GCAGC 2 cut(s) 107, 891
BshFI GGCC 2 cut(s) 531, 759
BsiHKAI GWGCWC 2 cut(s) 735, 970
BsiHKCI CYCGRG 1 cut(s) 1530
BslFI GGGAC 1 cut(s) 1294
BslI CCNNNNNNNGG 2 cut(s) 256, 1640
BsmAI GTCTC 6 cut(s) 291, 335, 560, 623, 1288, 1575
BsmFI GGGAC 1 cut(s) 1294
BsnI GGCC 2 cut(s) 531, 759
Bso31I GGTCTC 3 cut(s) 335, 560, 623
BsoBI CYCGRG 1 cut(s) 1530
Bsp1286I GDGCHC 2 cut(s) 735, 970
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 6 cut(s) 278, 436, 1015, 1172, 1435, 1615
Bsp19I CCATGG 1 cut(s) 516
BspACI CCGC 2 cut(s) 445, 1587
BspANI GGCC 2 cut(s) 531, 759
BspCNI CTCAG 6 cut(s) 331, 403, 982, 1021, 1282, 1585
BspHI TCATGA 2 cut(s) 187, 1246
BspMAI CTGCAG 2 cut(s) 498, 678
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 1 cut(s) 1623
BspTNI GGTCTC 3 cut(s) 335, 560, 623
BsrDI GCAATG 2 cut(s) 360, 1117
BsrGI TGTACA 1 cut(s) 417
BsrI ACTGG 4 cut(s) 318, 1216, 1321, 1496
BssECI CCNNGG 2 cut(s) 516, 1277
BssMI GATC 6 cut(s) 278, 436, 1015, 1172, 1435, 1615
BssT1I CCWWGG 1 cut(s) 516
Bst2UI CCWGG 3 cut(s) 552, 615, 1278
Bst4CI ACNGT 4 cut(s) 268, 285, 571, 634
Bst6I CTCTTC 3 cut(s) 830, 920, 933
BstAUI TGTACA 1 cut(s) 417
BstC8I GCNNGC 3 cut(s) 332, 529, 725
BstDEI CTNAG 8 cut(s) 63, 230, 339, 390, 969, 1029, 1290, 1572
BstDSI CCRYGG 1 cut(s) 516
BstF5I GGATG 8 cut(s) 322, 610, 685, 931, 1060, 1093, 1207, 1501
BstKTI GATC 6 cut(s) 281, 439, 1018, 1175, 1438, 1618
BstMAI GTCTC 6 cut(s) 291, 335, 560, 623, 1288, 1575
BstMBI GATC 6 cut(s) 278, 436, 1015, 1172, 1435, 1615
BstMWI GCNNNNNNNGC 1 cut(s) 1348
BstNI CCWGG 3 cut(s) 552, 615, 1278
BstNSI RCATGY 2 cut(s) 892, 1346
BstSCI CCNGG 3 cut(s) 550, 613, 1276
BstSFI CTRYAG 2 cut(s) 494, 674
BstV1I GCAGC 2 cut(s) 107, 891
BstV2I GAAGAC 1 cut(s) 752
BstX2I RGATCY 3 cut(s) 1015, 1172, 1615
BstXI CCANNNNNNTGG 2 cut(s) 517, 1133
BstYI RGATCY 3 cut(s) 1015, 1172, 1615
BsuI GTATCC 2 cut(s) 838, 1318
BsuRI GGCC 2 cut(s) 531, 759
BtgI CCRYGG 1 cut(s) 516
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 8 cut(s) 322, 610, 685, 931, 1060, 1093, 1207, 1501
BtsIMutI CAGTG 3 cut(s) 543, 1209, 1314
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 3 cut(s) 332, 529, 725
CciI TCATGA 2 cut(s) 187, 1246
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 4 cut(s) 311, 377, 418, 480
CviQI GTAC 4 cut(s) 311, 377, 418, 480
DdeI CTNAG 8 cut(s) 63, 230, 339, 390, 969, 1029, 1290, 1572
DpnI GATC 6 cut(s) 280, 438, 1017, 1174, 1437, 1617
DpnII GATC 6 cut(s) 278, 436, 1015, 1172, 1435, 1615
Eam1104I CTCTTC 3 cut(s) 830, 920, 933
EarI CTCTTC 3 cut(s) 830, 920, 933
Ecl136II GAGCTC 1 cut(s) 733
Eco130I CCWWGG 1 cut(s) 516
Eco147I AGGCCT 1 cut(s) 531
Eco24I GRGCYC 1 cut(s) 735
Eco31I GGTCTC 3 cut(s) 335, 560, 623
Eco53kI GAGCTC 1 cut(s) 733
Eco57I CTGAAG 1 cut(s) 789
Eco88I CYCGRG 1 cut(s) 1530
EcoICRI GAGCTC 1 cut(s) 733
EcoRII CCWGG 3 cut(s) 550, 613, 1276
EcoT14I CCWWGG 1 cut(s) 516
EcoT38I GRGCYC 1 cut(s) 735
ErhI CCWWGG 1 cut(s) 516
FalI AAGNNNNNCTT 2 cut(s) 707, 739
FaqI GGGAC 1 cut(s) 1294
FauNDI CATATG 2 cut(s) 1036, 1449
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 2 cut(s) 96, 905
FokI GGATG 8 cut(s) 329, 597, 692, 938, 1067, 1100, 1214, 1508
FriOI GRGCYC 1 cut(s) 735
Fsp4HI GCNGC 2 cut(s) 96, 905
FspBI CTAG 5 cut(s) 506, 704, 728, 1019, 1107
GluI GCNGC 2 cut(s) 96, 905
HaeIII GGCC 2 cut(s) 531, 759
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 6 cut(s) 152, 500, 912, 1187, 1538, 1644
HphI GGTGA 5 cut(s) 65, 613, 848, 1106, 1529
Hpy188I TCNGA 8 cut(s) 149, 251, 340, 472, 855, 1183, 1411, 1512
HpyAV CCTTC 2 cut(s) 345, 1635
HpyCH4III ACNGT 4 cut(s) 268, 285, 571, 634
HpyCH4IV ACGT 1 cut(s) 292
HpyF10VI GCNNNNNNNGC 1 cut(s) 1348
HpyF3I CTNAG 8 cut(s) 63, 230, 339, 390, 969, 1029, 1290, 1572
HpySE526I ACGT 1 cut(s) 292
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 6 cut(s) 278, 436, 1015, 1172, 1435, 1615
LmnI GCTCC 1 cut(s) 783
Lsp1109I GCAGC 2 cut(s) 107, 891
LweI GCATC 2 cut(s) 453, 754
MabI ACCWGGT 1 cut(s) 550
MaeI CTAG 5 cut(s) 506, 704, 728, 1019, 1107
MaeII ACGT 1 cut(s) 292
MalI GATC 6 cut(s) 280, 438, 1017, 1174, 1437, 1617
MboI GATC 6 cut(s) 278, 436, 1015, 1172, 1435, 1615
MfeI CAATTG 1 cut(s) 440
MflI RGATCY 3 cut(s) 1015, 1172, 1615
MhlI GDGCHC 2 cut(s) 735, 970
MlyI GAGTC 1 cut(s) 906
MmeI TCCRAC 1 cut(s) 1434
MseI TTAA 5 cut(s) 210, 369, 801, 920, 1655
MslI CAYNNNNRTG 6 cut(s) 40, 192, 231, 1131, 1182, 1319
MspR9I CCNGG 3 cut(s) 552, 615, 1278
MunI CAATTG 1 cut(s) 440
MvaI CCWGG 3 cut(s) 552, 615, 1278
MwoI GCNNNNNNNGC 1 cut(s) 1348
NcoI CCATGG 1 cut(s) 516
NdeI CATATG 2 cut(s) 1036, 1449
NdeII GATC 6 cut(s) 278, 436, 1015, 1172, 1435, 1615
NmeAIII GCCGAG 1 cut(s) 785
NmuCI GTSAC 4 cut(s) 233, 455, 891, 1166
NspI RCATGY 2 cut(s) 892, 1346
PaeR7I CTCGAG 1 cut(s) 1530
PagI TCATGA 2 cut(s) 187, 1246
PceI AGGCCT 1 cut(s) 531
PciI ACATGT 1 cut(s) 888
PfeI GAWTC 5 cut(s) 152, 500, 1187, 1538, 1644
PflFI GACNNNGTC 1 cut(s) 1567
PflMI CCANNNNNTGG 1 cut(s) 1640
PkrI GCNGC 2 cut(s) 97, 906
PleI GAGTC 1 cut(s) 906
PpsI GAGTC 1 cut(s) 906
PscI ACATGT 1 cut(s) 888
Psp124BI GAGCTC 1 cut(s) 735
Psp6I CCWGG 3 cut(s) 550, 613, 1276
PspGI CCWGG 3 cut(s) 550, 613, 1276
PstI CTGCAG 2 cut(s) 498, 678
PsuI RGATCY 3 cut(s) 1015, 1172, 1615
PsyI GACNNNGTC 1 cut(s) 1567
RsaI GTAC 4 cut(s) 312, 378, 419, 481
RsaNI GTAC 4 cut(s) 311, 377, 418, 480
RseI CAYNNNNRTG 6 cut(s) 40, 192, 231, 1131, 1182, 1319
SacI GAGCTC 1 cut(s) 735
SaqAI TTAA 5 cut(s) 210, 369, 801, 920, 1655
SatI GCNGC 2 cut(s) 96, 905
Sau3AI GATC 6 cut(s) 278, 436, 1015, 1172, 1435, 1615
SchI GAGTC 1 cut(s) 906
ScrFI CCNGG 3 cut(s) 552, 615, 1278
SduI GDGCHC 2 cut(s) 735, 970
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 2 cut(s) 453, 754
SfcI CTRYAG 2 cut(s) 494, 674
Sfr274I CTCGAG 1 cut(s) 1530
SlaI CTCGAG 1 cut(s) 1530
SmiMI CAYNNNNRTG 6 cut(s) 40, 192, 231, 1131, 1182, 1319
SmlI CTYRAG 2 cut(s) 323, 1530
SmoI CTYRAG 2 cut(s) 323, 1530
SseBI AGGCCT 1 cut(s) 531
SsiI CCGC 2 cut(s) 445, 1587
SspMI CTAG 5 cut(s) 506, 704, 728, 1019, 1107
SstI GAGCTC 1 cut(s) 735
StuI AGGCCT 1 cut(s) 531
StyD4I CCNGG 3 cut(s) 550, 613, 1276
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 4 cut(s) 268, 285, 571, 634
TaiI ACGT 1 cut(s) 295
TaqI TCGA 8 cut(s) 6, 566, 629, 735, 828, 1347, 1531, 1647
TatI WGTACW 2 cut(s) 376, 417
TfiI GAWTC 5 cut(s) 152, 500, 1187, 1538, 1644
Tru1I TTAA 5 cut(s) 210, 369, 801, 920, 1655
Tru9I TTAA 5 cut(s) 210, 369, 801, 920, 1655
TscAI CASTG 3 cut(s) 543, 1216, 1321
TseFI GTSAC 4 cut(s) 233, 455, 891, 1166
TseI GCWGC 2 cut(s) 95, 904
Tsp45I GTSAC 4 cut(s) 233, 455, 891, 1166
TspDTI ATGAA 8 cut(s) 149, 504, 680, 942, 1071, 1218, 1314, 1617
TspRI CASTG 3 cut(s) 543, 1216, 1321
Tth111I GACNNNGTC 1 cut(s) 1567
Van91I CCANNNNNTGG 1 cut(s) 1640
XapI RAATTY 4 cut(s) 167, 244, 578, 641
XbaI TCTAGA 1 cut(s) 1018
XceI RCATGY 2 cut(s) 892, 1346
XcmI CCANNNNNNNNNTGG 1 cut(s) 1439
XhoI CTCGAG 1 cut(s) 1530
XspI CTAG 5 cut(s) 506, 704, 728, 1019, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.