Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0003395

Physical Location & Seq

Genomic Coordinates Reverse (-)

32826244 .. 32829376

Gene Structure 3133 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G025050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1890 bp

ATGGAAAACGTTGTGTTCTACCAAACGGATTGCGAGACCCTTTGGTCTCAGATTAAATGCAAAGAGAAGCAGATTCAACTCAAACGGAGGTGGTTGTTGGGTCTTCCTCTCTCTGAATCTGAGCACAACCTGTTTCAAAGTCCTGACCTTGAACGAGATTCATTGCCGGAATCTTTGCTCAGGGAAGATGATCTGTTCTATGAGACTGCAAAGTCTTCTGTTCAAGCAGCCTTTGGAGCCTGCAATGTTGAAGCAGGGAAGCAAGATAATATTCTAATGCTCGACAGAAGAAGTATATCCACAGCCTTGTCATCGCCATGTCTTGATGATTTGACTACTAAGGGGCTTCACCTTATAGCTACTACACTTGCAGCAGGTTCAGTCAAGTTTGAGAACACTAGTTGCGAGATGAAGAAGTTTATCAGAGAATCTCTGCAAAAAAAATTTAGAAGCCAAAATAATCATGACGATGAGTTGGTCATTTTAAAGCAACTATCTCAACATCTTAGTGACCCACAAAATTTCCGAGATAAGGTTGTTACCGTTCTTTCCAGATCACAGTCTCAACGTCTTGCTGTTAGAAAGGTACTGGATGGACTTGAGGACTTGCCTTCTGAGACCCTATTTGCAATGAGCAGAAAGCTTAAAGGAGTACAACATTTACCTCAGTTACATCAAAAGAAAAATACATTGTACAAAAAGACTTTGATTGATCAATTGCGGAAAACTTGTGACACGATGCTTTCCGAACTTGGTACAGGAGATGAACTGCAGGAATCACTAGCCAAAGCCATGGCAGTAGCAGGCCTATCAGTGAAGTTACAACCAGGTCTCCATAATTCGACTGTAACAGAATTTCACCGATTTTCACAAGAGATAAAGATACTGCAGGATGATATTGCAAGGGCTATTTGGCTAGTTGAAAAAAAGGTTAGCTTGCTAGAGCTCGAAAAGTTGAAGACTATTTTAGGCCGAGATGCTGAAGTATCAAACAGGAGCTTACGATATGCAATTAAGAAAATGCTAACTGAATATCTCTTCGAGTGTGGTGATATGGATACAATTCCGAAGTCATTATTAGAAGCTCTTGATATTATCAACATGTCACGAAACAAGCTGCATAGACTCTTCTTAAAGGATGAAATTGAAGAAGAGGTGGAATGTATATTGAATGTGAGTGCTCAGGCAAAGCAGATTGTATGGGATATGTTCCCCGACCATGAGTTAGATCTAGACTTTGCTGAGGCATATGTTGAGGAAATGGAGGATGAAGACGATGATGAGGATGACGATGATGATGGTGATGATAGGTCTAGCAATGATGATAACAACCATAATCATGGTTCGCCACAGGAACAAGGAATATCTGGAAGTGACAGATCTCACTCTGATGATTCACATTATGAGGATGAAAGCACTGGGGAAACAATGTTCTCCAATCAAGGAACTTCTCATGATTTCAATGGTATTAGACGAGGGTTTCCTGGGACTTCTACTGAGAGACATGAACCTGAACATGACACTGGAATGGATACAGATGACCCTTTGCATGTCGAGCCAGAGCCAGATGATGTGCATAAACAAACCACTTGTAACCAATACCTTGCAATTCAAGAAGTGTCTGATAAGACAAGCATGATAGCCCATGATCTTGTTGGTCATATGTTGGAGGAGTTTGCACAGAAAGATGGCTTGGATTTGGACTGGGATGATACATTATATCTGAAAGGTGATTGTGCAACTCGAGAAGATTCAGGAGTGGTAGAAGAAAGGCAGACATTGTCTCAGGAGAACAAAGGCGGTTCTGTAATTGTTAGTGTAATTGAGGATCTAATACCTTCATTTCCAAAGAGTGGAATCGAAGCATTAAGGAACTTGGTGGGCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

629

Amino Acids

71.29

Weight (kDa)

4.74

Isoelectric Point (pI)

55.16

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003395)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40520 AT5G40520
fragaria_vesca	FvH4_1g27220 FvH4_1g27220 FvH4_1g27220 FvH4_1g27220 FvH4_1g27220 FvH4_1g27220 FvH4_1g27220 FvH4_1g27220 FvH4_1g27220 FvH4_1g27220 FvH4_1g27220
malus_domestica	MD04G1046800.v1.1
prunus_persica	Prupe.8G045700_v2.0.a1 Prupe.8G045700_v2.0.a1 Prupe.8G045700_v2.0.a1 Prupe.8G045700_v2.0.a1 Prupe.8G045700_v2.0.a1 Prupe.8G045700_v2.0.a1 Prupe.8G045700_v2.0.a1 Prupe.8G045700_v2.0.a1 Prupe.8G045700_v2.0.a1
pyrus_communis	pycom04g04060
rosa_chinensis	RchiOBHm_Chr3g0487611
rosa_laevigata	RLG00000022996
rosa_multiflora	Rmu_sc0012412.1_g000009
rosa_roxburghii	Rroxscaffold_6G00394650
rosa_rugosa	Rorug03G0235000 Rorug03G0235100 Rorug03G0235100
rosa_samantha	Rh3AG283300 Rh3BG319000 Rh3CG317100 Rh3DG315300
rosa_wichuraiana	Rw3G025050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	43, 211
Acc36I	ACCTGC	1 cut(s)	365
AccB7I	CCANNNNNTGG	1 cut(s)	1853
AciI	CCGC	2 cut(s)	721, 1800
AclI	AACGTT	1 cut(s)	9
AclWI	GGATC	1 cut(s)	1836
AcsI	RAATTY	3 cut(s)	443, 520, 854
AcuI	CTGAAG	1 cut(s)	1002
AfaI	GTAC	4 cut(s)	588, 654, 695, 757
AfiI	CCNNNNNNNGG	2 cut(s)	532, 1853
AflIII	ACRYGT	1 cut(s)	1101
AhlI	ACTAGT	1 cut(s)	398
AjnI	CCWGG	2 cut(s)	826, 1483
AjuI	GAANNNNNNNTTGG	2 cut(s)	1676, 1708
AloI	GAACNNNNNNTCC	2 cut(s)	1415, 1447
AluBI	AGCT	7 cut(s)	359, 643, 936, 946, 999, 1085, 1117
AluI	AGCT	7 cut(s)	359, 643, 936, 946, 999, 1085, 1117
Alw21I	GWGCWC	3 cut(s)	126, 948, 1183
Alw26I	GTCTC	8 cut(s)	29, 51, 197, 567, 611, 836, 1495, 1788
AlwI	GGATC	1 cut(s)	1836
Ama87I	CYCGRG	1 cut(s)	1743
AoxI	GGCC	2 cut(s)	805, 970
ApeKI	GCWGC	3 cut(s)	227, 371, 1117
ApoI	RAATTY	3 cut(s)	443, 520, 854
AsuHPI	GGTGA	5 cut(s)	341, 851, 1061, 1313, 1742
AvaI	CYCGRG	1 cut(s)	1743
BanII	GRGCYC	1 cut(s)	948
BbsI	GAAGAC	4 cut(s)	95, 207, 965, 1278
Bbv12I	GWGCWC	3 cut(s)	126, 948, 1183
BbvCI	CCTCAGC	1 cut(s)	1242
BbvI	GCAGC	3 cut(s)	239, 383, 1104
BccI	CCATC	3 cut(s)	587, 1292, 1682
BciT130I	CCWGG	2 cut(s)	828, 1485
BciVI	GTATCC	2 cut(s)	1051, 1525
BclI	TGATCA	1 cut(s)	712
BcoDI	GTCTC	8 cut(s)	29, 51, 197, 567, 611, 836, 1495, 1788
BcuI	ACTAGT	1 cut(s)	398
BfaI	CTAG	6 cut(s)	399, 782, 917, 941, 1232, 1314
BfmI	CTRYAG	2 cut(s)	770, 887
BfuAI	ACCTGC	1 cut(s)	365
BfuI	GTATCC	2 cut(s)	1051, 1525
BglII	AGATCT	2 cut(s)	1228, 1379
BisI	GCNGC	3 cut(s)	228, 372, 1118
BlsI	GCNGC	3 cut(s)	229, 373, 1119
Bme1390I	CCNGG	2 cut(s)	828, 1485
BmeT110I	CYCGRG	1 cut(s)	1743
BmiI	GGNNCC	1 cut(s)	238
BmrFI	CCNGG	2 cut(s)	828, 1485
BmrI	ACTGGG	2 cut(s)	1428, 1714
BmsI	GCATC	2 cut(s)	729, 967
BmuI	ACTGGG	2 cut(s)	1428, 1714
BpiI	GAAGAC	4 cut(s)	95, 207, 965, 1278
Bpu10I	CCTNAGC	3 cut(s)	179, 1182, 1242
BpuEI	CTTGAG	1 cut(s)	620
BsaI	GGTCTC	4 cut(s)	29, 51, 611, 836
BsaJI	CCNNGG	2 cut(s)	792, 1484
BsaXI	ACNNNNNCTCC	2 cut(s)	816, 846
Bsc4I	CCNNNNNNNGG	2 cut(s)	532, 1853
Bse1I	ACTGG	4 cut(s)	594, 1423, 1528, 1709
Bse3DI	GCAATG	4 cut(s)	161, 250, 636, 1324
BseBI	CCWGG	2 cut(s)	828, 1485
BseDI	CCNNGG	2 cut(s)	792, 1484
BseGI	GGATG	7 cut(s)	598, 898, 1144, 1273, 1291, 1414, 1714
BseLI	CCNNNNNNNGG	2 cut(s)	532, 1853
BseMI	GCAATG	4 cut(s)	161, 250, 636, 1324
BseMII	CTCAG	9 cut(s)	62, 111, 193, 606, 680, 1196, 1233, 1488, 1799
BseNI	ACTGG	4 cut(s)	594, 1423, 1528, 1709
BseRI	GAGGAG	1 cut(s)	1685
BseXI	GCAGC	3 cut(s)	239, 383, 1104
BshFI	GGCC	2 cut(s)	807, 972
BsiHKAI	GWGCWC	3 cut(s)	126, 948, 1183
BsiHKCI	CYCGRG	1 cut(s)	1743
BsiSI	CCGG	1 cut(s)	167
BslFI	GGGAC	1 cut(s)	1501
BslI	CCNNNNNNNGG	2 cut(s)	532, 1853
BsmAI	GTCTC	8 cut(s)	29, 51, 197, 567, 611, 836, 1495, 1788
BsmFI	GGGAC	1 cut(s)	1501
BsnI	GGCC	2 cut(s)	807, 972
Bso31I	GGTCTC	4 cut(s)	29, 51, 611, 836
BsoBI	CYCGRG	1 cut(s)	1743
Bsp1286I	GDGCHC	3 cut(s)	126, 948, 1183
Bsp1407I	TGTACA	1 cut(s)	693
Bsp143I	GATC	7 cut(s)	190, 554, 712, 1228, 1379, 1648, 1828
Bsp19I	CCATGG	1 cut(s)	792
BspACI	CCGC	2 cut(s)	721, 1800
BspANI	GGCC	2 cut(s)	807, 972
BspCNI	CTCAG	9 cut(s)	61, 112, 192, 607, 679, 1195, 1234, 1489, 1798
BspHI	TCATGA	2 cut(s)	463, 1453
BspLI	GGNNCC	1 cut(s)	238
BspMAI	CTGCAG	2 cut(s)	774, 891
BspMI	ACCTGC	1 cut(s)	365
BspPI	GGATC	1 cut(s)	1836
BspTNI	GGTCTC	4 cut(s)	29, 51, 611, 836
BsrDI	GCAATG	4 cut(s)	161, 250, 636, 1324
BsrGI	TGTACA	1 cut(s)	693
BsrI	ACTGG	4 cut(s)	594, 1423, 1528, 1709
BssECI	CCNNGG	2 cut(s)	792, 1484
BssMI	GATC	7 cut(s)	190, 554, 712, 1228, 1379, 1648, 1828
BssT1I	CCWWGG	1 cut(s)	792
Bst2UI	CCWGG	2 cut(s)	828, 1485
Bst4CI	ACNGT	3 cut(s)	544, 561, 847
Bst6I	CTCTTC	3 cut(s)	1043, 1133, 1146
BstAUI	TGTACA	1 cut(s)	693
BstC8I	GCNNGC	3 cut(s)	241, 805, 938
BstDSI	CCRYGG	1 cut(s)	792
BstF5I	GGATG	7 cut(s)	598, 898, 1144, 1273, 1291, 1414, 1714
BstKTI	GATC	7 cut(s)	193, 557, 715, 1231, 1382, 1651, 1831
BstMAI	GTCTC	8 cut(s)	29, 51, 197, 567, 611, 836, 1495, 1788
BstMBI	GATC	7 cut(s)	190, 554, 712, 1228, 1379, 1648, 1828
BstMWI	GCNNNNNNNGC	2 cut(s)	236, 1555
BstNI	CCWGG	2 cut(s)	828, 1485
BstNSI	RCATGY	2 cut(s)	1105, 1553
BstSCI	CCNGG	2 cut(s)	826, 1483
BstSFI	CTRYAG	2 cut(s)	770, 887
BstV1I	GCAGC	3 cut(s)	239, 383, 1104
BstV2I	GAAGAC	4 cut(s)	95, 207, 965, 1278
BstX2I	RGATCY	3 cut(s)	1228, 1379, 1828
BstXI	CCANNNNNNTGG	2 cut(s)	793, 1340
BstYI	RGATCY	3 cut(s)	1228, 1379, 1828
BsuI	GTATCC	2 cut(s)	1051, 1525
BsuRI	GGCC	2 cut(s)	807, 972
BtgI	CCRYGG	1 cut(s)	792
BtgZI	GCGATG	1 cut(s)	297
BtsCI	GGATG	7 cut(s)	598, 898, 1144, 1273, 1291, 1414, 1714
BtsIMutI	CAGTG	3 cut(s)	819, 1416, 1521
BveI	ACCTGC	1 cut(s)	365
Cac8I	GCNNGC	3 cut(s)	241, 805, 938
CciI	TCATGA	2 cut(s)	463, 1453
CsiI	ACCWGGT	1 cut(s)	826
Csp6I	GTAC	4 cut(s)	587, 653, 694, 756
CviQI	GTAC	4 cut(s)	587, 653, 694, 756
DpnI	GATC	7 cut(s)	192, 556, 714, 1230, 1381, 1650, 1830
DpnII	GATC	7 cut(s)	190, 554, 712, 1228, 1379, 1648, 1828
DraI	TTTAAA	1 cut(s)	486
DrdI	GACNNNNNNGTC	2 cut(s)	43, 211
DseDI	GACNNNNNNGTC	2 cut(s)	43, 211
Eam1104I	CTCTTC	3 cut(s)	1043, 1133, 1146
EarI	CTCTTC	3 cut(s)	1043, 1133, 1146
Ecl136II	GAGCTC	1 cut(s)	946
Eco130I	CCWWGG	1 cut(s)	792
Eco147I	AGGCCT	1 cut(s)	807
Eco24I	GRGCYC	1 cut(s)	948
Eco31I	GGTCTC	4 cut(s)	29, 51, 611, 836
Eco53kI	GAGCTC	1 cut(s)	946
Eco57I	CTGAAG	1 cut(s)	1002
Eco88I	CYCGRG	1 cut(s)	1743
EcoICRI	GAGCTC	1 cut(s)	946
EcoRII	CCWGG	2 cut(s)	826, 1483
EcoT14I	CCWWGG	1 cut(s)	792
EcoT38I	GRGCYC	1 cut(s)	948
ErhI	CCWWGG	1 cut(s)	792
FalI	AAGNNNNNCTT	2 cut(s)	920, 952
FaqI	GGGAC	1 cut(s)	1501
FauNDI	CATATG	2 cut(s)	1249, 1662
FbaI	TGATCA	1 cut(s)	712
Fnu4HI	GCNGC	3 cut(s)	228, 372, 1118
FokI	GGATG	7 cut(s)	605, 905, 1151, 1280, 1298, 1421, 1721
FriOI	GRGCYC	1 cut(s)	948
Fsp4HI	GCNGC	3 cut(s)	228, 372, 1118
FspBI	CTAG	6 cut(s)	399, 782, 917, 941, 1232, 1314
GluI	GCNGC	3 cut(s)	228, 372, 1118
HaeIII	GGCC	2 cut(s)	807, 972
HapII	CCGG	1 cut(s)	167
HindIII	AAGCTT	1 cut(s)	641
HpaII	CCGG	1 cut(s)	167
HphI	GGTGA	5 cut(s)	341, 851, 1061, 1313, 1742
HpyAV	CCTTC	2 cut(s)	621, 1848
HpyCH4III	ACNGT	3 cut(s)	544, 561, 847
HpyCH4IV	ACGT	2 cut(s)	9, 568
HpyF10VI	GCNNNNNNNGC	2 cut(s)	236, 1555
HpySE526I	ACGT	2 cut(s)	9, 568
Ksp22I	TGATCA	1 cut(s)	712
Kzo9I	GATC	7 cut(s)	190, 554, 712, 1228, 1379, 1648, 1828
LmnI	GCTCC	2 cut(s)	236, 996
Lsp1109I	GCAGC	3 cut(s)	239, 383, 1104
LweI	GCATC	2 cut(s)	729, 967
MabI	ACCWGGT	1 cut(s)	826
MaeI	CTAG	6 cut(s)	399, 782, 917, 941, 1232, 1314
MaeII	ACGT	2 cut(s)	9, 568
MaeIII	GTNAC	9 cut(s)	509, 538, 669, 731, 819, 847, 1104, 1373, 1592
MalI	GATC	7 cut(s)	192, 556, 714, 1230, 1381, 1650, 1830
MboI	GATC	7 cut(s)	190, 554, 712, 1228, 1379, 1648, 1828
MfeI	CAATTG	1 cut(s)	716
MflI	RGATCY	3 cut(s)	1228, 1379, 1828
MhlI	GDGCHC	3 cut(s)	126, 948, 1183
MlyI	GAGTC	1 cut(s)	1119
MmeI	TCCRAC	1 cut(s)	1647
MseI	TTAA	6 cut(s)	54, 485, 645, 1014, 1133, 1868
MslI	CAYNNNNRTG	6 cut(s)	316, 468, 507, 1338, 1389, 1526
MspI	CCGG	1 cut(s)	167
MspR9I	CCNGG	2 cut(s)	828, 1485
MunI	CAATTG	1 cut(s)	716
MvaI	CCWGG	2 cut(s)	828, 1485
MwoI	GCNNNNNNNGC	2 cut(s)	236, 1555
NcoI	CCATGG	1 cut(s)	792
NdeI	CATATG	2 cut(s)	1249, 1662
NdeII	GATC	7 cut(s)	190, 554, 712, 1228, 1379, 1648, 1828
NlaIV	GGNNCC	1 cut(s)	238
NmeAIII	GCCGAG	1 cut(s)	998
NmuCI	GTSAC	4 cut(s)	509, 731, 1104, 1373
NspI	RCATGY	2 cut(s)	1105, 1553
PaeR7I	CTCGAG	1 cut(s)	1743
PagI	TCATGA	2 cut(s)	463, 1453
PceI	AGGCCT	1 cut(s)	807
PciI	ACATGT	1 cut(s)	1101
PfeI	GAWTC	9 cut(s)	73, 116, 158, 170, 428, 776, 1394, 1751, 1857
PflFI	GACNNNGTC	1 cut(s)	1780
PflMI	CCANNNNNTGG	1 cut(s)	1853
PkrI	GCNGC	3 cut(s)	229, 373, 1119
PleI	GAGTC	1 cut(s)	1119
PpsI	GAGTC	1 cut(s)	1119
PscI	ACATGT	1 cut(s)	1101
Psp124BI	GAGCTC	1 cut(s)	948
Psp1406I	AACGTT	1 cut(s)	9
Psp6I	CCWGG	2 cut(s)	826, 1483
PspGI	CCWGG	2 cut(s)	826, 1483
PspN4I	GGNNCC	1 cut(s)	238
PstI	CTGCAG	2 cut(s)	774, 891
PsuI	RGATCY	3 cut(s)	1228, 1379, 1828
PsyI	GACNNNGTC	1 cut(s)	1780
RsaI	GTAC	4 cut(s)	588, 654, 695, 757
RsaNI	GTAC	4 cut(s)	587, 653, 694, 756
RseI	CAYNNNNRTG	6 cut(s)	316, 468, 507, 1338, 1389, 1526
SacI	GAGCTC	1 cut(s)	948
SaqAI	TTAA	6 cut(s)	54, 485, 645, 1014, 1133, 1868
SatI	GCNGC	3 cut(s)	228, 372, 1118
Sau3AI	GATC	7 cut(s)	190, 554, 712, 1228, 1379, 1648, 1828
SchI	GAGTC	1 cut(s)	1119
ScrFI	CCNGG	2 cut(s)	828, 1485
SduI	GDGCHC	3 cut(s)	126, 948, 1183
SexAI	ACCWGGT	1 cut(s)	826
SfaNI	GCATC	2 cut(s)	729, 967
SfcI	CTRYAG	2 cut(s)	770, 887
Sfr274I	CTCGAG	1 cut(s)	1743
SlaI	CTCGAG	1 cut(s)	1743
SmiMI	CAYNNNNRTG	6 cut(s)	316, 468, 507, 1338, 1389, 1526
SmlI	CTYRAG	2 cut(s)	599, 1743
SmoI	CTYRAG	2 cut(s)	599, 1743
SpeI	ACTAGT	1 cut(s)	398
SseBI	AGGCCT	1 cut(s)	807
SsiI	CCGC	2 cut(s)	721, 1800
SspI	AATATT	1 cut(s)	271
SspMI	CTAG	6 cut(s)	399, 782, 917, 941, 1232, 1314
SstI	GAGCTC	1 cut(s)	948
StuI	AGGCCT	1 cut(s)	807
StyD4I	CCNGG	2 cut(s)	826, 1483
StyI	CCWWGG	1 cut(s)	792
TaaI	ACNGT	3 cut(s)	544, 561, 847
TaiI	ACGT	2 cut(s)	12, 571
TaqI	TCGA	7 cut(s)	282, 842, 948, 1041, 1554, 1744, 1860
TatI	WGTACW	2 cut(s)	652, 693
TfiI	GAWTC	9 cut(s)	73, 116, 158, 170, 428, 776, 1394, 1751, 1857
Tru1I	TTAA	6 cut(s)	54, 485, 645, 1014, 1133, 1868
Tru9I	TTAA	6 cut(s)	54, 485, 645, 1014, 1133, 1868
TscAI	CASTG	3 cut(s)	819, 1423, 1528
TseFI	GTSAC	4 cut(s)	509, 731, 1104, 1373
TseI	GCWGC	3 cut(s)	227, 371, 1117
Tsp45I	GTSAC	4 cut(s)	509, 731, 1104, 1373
TspDTI	ATGAA	8 cut(s)	150, 425, 780, 1155, 1284, 1425, 1521, 1830
TspGWI	ACGGA	2 cut(s)	41, 100
TspRI	CASTG	3 cut(s)	819, 1423, 1528
Tth111I	GACNNNGTC	1 cut(s)	1780
Van91I	CCANNNNNTGG	1 cut(s)	1853
XapI	RAATTY	3 cut(s)	443, 520, 854
XbaI	TCTAGA	1 cut(s)	1231
XceI	RCATGY	2 cut(s)	1105, 1553
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1652
XhoI	CTCGAG	1 cut(s)	1743
XspI	CTAG	6 cut(s)	399, 782, 917, 941, 1232, 1314

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.