Rh3DG168300

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0009919
Physical Location & Seq
Reverse (-)
14677537 .. 14681081
3545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG168300.1

Sequence Viewer

Length: 849 bp
ATGAATGAGTCGTTGTTGAACTCGCGTTTGATGTTCATACGAGCCGCCATGAAAAACCTAGAAGCCAACCAATTTGGGACCAGTCAGGCTAGAACTACAGACCATATGCCTTTCCTAATTGTACTGAGGACAAGCATTGTTGCAATGCAGGTTTCCCTAGATACAAGCATGCATGTCATGGTGTTGGAATTGCTTTGTAGTGAGGGTTGGATTCTCGCTTTAACTCCTCCCCCCCTCCTCCCCCTTATTCTTATCCCATTCCCTTCTCCCCGTACTTTCCCCAATTCTCTCAAACCCTCTCAGATCAGCGTTTCGCAGATCGATCTAGGCGATCTAATATTCGGTGGGATTTCTGGTTCGAATTTTGAAGAGATCTTGAAGCAGAGCAGAGAGTCTCGAGATGGCGGAAGAGCACAGATGCCAAGCACCAGAAGAGACTTTTGCCTCAAGGAGCAACAGGAGGCCTCCATCAAATCCACCGTCGAAGCCTCTCTCTCCGCCTCCGCGGCCGCCTCTTCGTCGGCCTCGTCGTCCTCCTCTTCTCCGGTGATTGACTTCCGCTCTCTCCCTCCGCCTCCGCTGTTGACTTTGCCGGAGGTCGCCGAGGCCGAAGATGTTCCCACTCCTCCAGAGGTCGCCGTCCCGGCGGTGTCGCAGCCGAACCGGTGCAACGTCTGCCGGAAACGGGTCGGGTTGACCGGGTTCAAGTGCAAGTGCGGGACCACCTTCTGCGGGGTCCACCGTTACCCGGAGAAGCACGCGTGCTCGTTCGACTTCAAGACGGTCGGGAAGGAGGAGATCGCGAGGAGCAATCCGGTGATCAAAGCAGAGAAGCTCGAGAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

282

Amino Acids

30.69

Weight (kDa)

8.11

Isoelectric Point (pI)

60.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AN1 PF01428 223 - 259 1.9e-09 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 139
AccBSI CCGCTC 1 cut(s) 561
AccII CGCG 4 cut(s) 25, 506, 761, 803
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 1 cut(s) 361
AfaI GTAC 2 cut(s) 123, 274
AfiI CCNNNNNNNGG 3 cut(s) 685, 732, 748
AflIII ACRYGT 1 cut(s) 759
AgeI ACCGGT 1 cut(s) 663
AgsI TTSAA 5 cut(s) 19, 368, 379, 706, 778
AloI GAACNNNNNNTCC 2 cut(s) 340, 372
AluBI AGCT 1 cut(s) 835
AluI AGCT 1 cut(s) 835
Alw21I GWGCWC 2 cut(s) 415, 767
Alw26I GTCTC 2 cut(s) 399, 429
Ama87I CYCGRG 2 cut(s) 396, 836
AoxI GGCC 4 cut(s) 462, 507, 522, 606
ApeKI GCWGC 1 cut(s) 655
ApoI RAATTY 1 cut(s) 361
ArsI GACNNNNNNTTYG 2 cut(s) 465, 497
AsiGI ACCGGT 1 cut(s) 663
Asp700I GAANNNNTTC 1 cut(s) 615
AspS9I GGNCC 3 cut(s) 78, 720, 736
AsuC2I CCSGG 3 cut(s) 644, 700, 749
AsuHPI GGTGA 2 cut(s) 559, 829
AsuII TTCGAA 1 cut(s) 359
AvaI CYCGRG 2 cut(s) 396, 836
AvaII GGWCC 3 cut(s) 78, 720, 736
Bbv12I GWGCWC 2 cut(s) 415, 767
BbvI GCAGC 1 cut(s) 667
BccI CCATC 2 cut(s) 395, 476
BceAI ACGGC 1 cut(s) 623
BclI TGATCA 1 cut(s) 819
BcnI CCSGG 3 cut(s) 644, 700, 749
BcoDI GTCTC 2 cut(s) 399, 429
BfaI CTAG 4 cut(s) 59, 90, 158, 326
BfmI CTRYAG 1 cut(s) 96
BfuAI ACCTGC 1 cut(s) 139
BglI GCCNNNNNGGC 2 cut(s) 506, 644
BglII AGATCT 1 cut(s) 372
BisI GCNGC 4 cut(s) 45, 507, 510, 656
BlsI GCNGC 4 cut(s) 46, 508, 511, 657
Bme1390I CCNGG 3 cut(s) 644, 700, 749
Bme18I GGWCC 3 cut(s) 78, 720, 736
BmeT110I CYCGRG 2 cut(s) 396, 836
BmgT120I GGNCC 3 cut(s) 78, 720, 736
BmiI GGNNCC 3 cut(s) 79, 721, 737
BmrFI CCNGG 3 cut(s) 644, 700, 749
BmsI GCATC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 612
Bpu14I TTCGAA 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 3 cut(s) 644, 700, 749
Bsa29I ATCGAT 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 504, 603
BsaWI WCCGGW 3 cut(s) 544, 663, 814
Bsc4I CCNNNNNNNGG 3 cut(s) 685, 732, 748
Bse118I RCCGGY 1 cut(s) 663
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 150
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 504, 603
BseLI CCNNNNNNNGG 3 cut(s) 685, 732, 748
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 2 cut(s) 116, 314
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 6 cut(s) 216, 227, 526, 615, 809, 820
BseX3I CGGCCG 1 cut(s) 507
BseXI GCAGC 1 cut(s) 667
Bsh1236I CGCG 4 cut(s) 25, 506, 761, 803
Bsh1285I CGRYCG 2 cut(s) 510, 786
BshFI GGCC 4 cut(s) 464, 509, 524, 608
BshTI ACCGGT 1 cut(s) 663
BshVI ATCGAT 1 cut(s) 321
BsiEI CGRYCG 2 cut(s) 510, 786
BsiHKAI GWGCWC 2 cut(s) 415, 767
BsiHKCI CYCGRG 2 cut(s) 396, 836
BsiSI CCGG 8 cut(s) 545, 593, 644, 664, 679, 699, 749, 815
BslFI GGGAC 3 cut(s) 91, 626, 733
BslI CCNNNNNNNGG 3 cut(s) 685, 732, 748
BsmAI GTCTC 2 cut(s) 399, 429
BsmFI GGGAC 3 cut(s) 91, 626, 733
BsnI GGCC 4 cut(s) 464, 509, 524, 608
BsoBI CYCGRG 2 cut(s) 396, 836
Bsp119I TTCGAA 1 cut(s) 359
Bsp1286I GDGCHC 2 cut(s) 415, 767
Bsp143I GATC 7 cut(s) 303, 318, 322, 331, 372, 798, 819
Bsp68I TCGCGA 1 cut(s) 803
BspANI GGCC 4 cut(s) 464, 509, 524, 608
BspCNI CTCAG 2 cut(s) 117, 313
BspDI ATCGAT 1 cut(s) 321
BspFNI CGCG 4 cut(s) 25, 506, 761, 803
BspLI GGNNCC 3 cut(s) 79, 721, 737
BspMI ACCTGC 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 403
BspT104I TTCGAA 1 cut(s) 359
BsrBI CCGCTC 1 cut(s) 561
BsrDI GCAATG 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 663
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 663
BssECI CCNNGG 2 cut(s) 504, 603
BssMI GATC 7 cut(s) 303, 318, 322, 331, 372, 798, 819
Bst4CI ACNGT 3 cut(s) 481, 743, 784
Bst6I CTCTTC 5 cut(s) 363, 403, 427, 520, 544
BstAPI GCANNNNNTGC 1 cut(s) 675
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 3 cut(s) 170, 759, 763
BstDEI CTNAG 2 cut(s) 125, 300
BstDSI CCRYGG 1 cut(s) 504
BstFNI CGCG 4 cut(s) 25, 506, 761, 803
BstKTI GATC 7 cut(s) 306, 321, 325, 334, 375, 801, 822
BstMAI GTCTC 2 cut(s) 399, 429
BstMBI GATC 7 cut(s) 303, 318, 322, 331, 372, 798, 819
BstMCI CGRYCG 2 cut(s) 510, 786
BstMWI GCNNNNNNNGC 3 cut(s) 506, 644, 675
BstNSI RCATGY 2 cut(s) 172, 176
BstSCI CCNGG 3 cut(s) 642, 698, 747
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 4 cut(s) 25, 506, 761, 803
BstV1I GCAGC 1 cut(s) 667
BstX2I RGATCY 1 cut(s) 372
BstYI RGATCY 1 cut(s) 372
BstZI CGGCCG 1 cut(s) 507
Bsu15I ATCGAT 1 cut(s) 321
BsuRI GGCC 4 cut(s) 464, 509, 524, 608
BsuTUI ATCGAT 1 cut(s) 321
BtgI CCRYGG 1 cut(s) 504
BtuMI TCGCGA 1 cut(s) 803
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 3 cut(s) 170, 759, 763
CciNI GCGGCCGC 1 cut(s) 507
Cfr10I RCCGGY 1 cut(s) 663
Cfr13I GGNCC 3 cut(s) 78, 720, 736
Cfr42I CCGCGG 1 cut(s) 507
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 2 cut(s) 122, 273
CspAI ACCGGT 1 cut(s) 663
CviAII CATG 4 cut(s) 49, 169, 173, 178
CviQI GTAC 2 cut(s) 122, 273
DdeI CTNAG 2 cut(s) 125, 300
DpnI GATC 7 cut(s) 305, 320, 324, 333, 374, 800, 821
DpnII GATC 7 cut(s) 303, 318, 322, 331, 372, 798, 819
EaeI YGGCCR 1 cut(s) 507
EagI CGGCCG 1 cut(s) 507
Eam1104I CTCTTC 5 cut(s) 363, 403, 427, 520, 544
EarI CTCTTC 5 cut(s) 363, 403, 427, 520, 544
EciI GGCGGA 3 cut(s) 420, 487, 561
EclXI CGGCCG 1 cut(s) 507
Eco147I AGGCCT 1 cut(s) 464
Eco47I GGWCC 3 cut(s) 78, 720, 736
Eco52I CGGCCG 1 cut(s) 507
Eco88I CYCGRG 2 cut(s) 396, 836
EcoT22I ATGCAT 1 cut(s) 174
FaeI CATG 4 cut(s) 52, 172, 176, 181
FaiI YATR 7 cut(s) 38, 50, 105, 107, 170, 174, 179
FaqI GGGAC 3 cut(s) 91, 626, 733
FatI CATG 4 cut(s) 48, 168, 172, 177
FauI CCCGC 2 cut(s) 710, 725
FauNDI CATATG 1 cut(s) 105
FbaI TGATCA 1 cut(s) 819
Fnu4HI GCNGC 4 cut(s) 45, 507, 510, 656
Fsp4HI GCNGC 4 cut(s) 45, 507, 510, 656
FspBI CTAG 4 cut(s) 59, 90, 158, 326
GluI GCNGC 4 cut(s) 45, 507, 510, 656
GsuI CTGGAG 1 cut(s) 612
HaeIII GGCC 4 cut(s) 464, 509, 524, 608
HapII CCGG 8 cut(s) 545, 593, 644, 664, 679, 699, 749, 815
Hin1II CATG 4 cut(s) 52, 172, 176, 181
HincII GTYRAC 2 cut(s) 585, 696
HindII GTYRAC 2 cut(s) 585, 696
HinfI GANTC 3 cut(s) 8, 211, 392
HpaII CCGG 8 cut(s) 545, 593, 644, 664, 679, 699, 749, 815
HphI GGTGA 2 cut(s) 559, 829
Hpy166II GTNNAC 3 cut(s) 585, 696, 739
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 8 cut(s) 376, 396, 398, 629, 778, 787, 802, 838
Hpy8I GTNNAC 3 cut(s) 585, 696, 739
Hpy99I CGWCG 3 cut(s) 485, 523, 532
HpyAV CCTTC 3 cut(s) 273, 736, 784
HpyCH4III ACNGT 3 cut(s) 481, 743, 784
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 5 cut(s) 143, 148, 172, 669, 711
HpyF10VI GCNNNNNNNGC 3 cut(s) 506, 644, 675
HpyF3I CTNAG 2 cut(s) 125, 300
HpySE526I ACGT 1 cut(s) 672
Hsp92II CATG 4 cut(s) 52, 172, 176, 181
Ksp22I TGATCA 1 cut(s) 819
KspI CCGCGG 1 cut(s) 507
Kzo9I GATC 7 cut(s) 303, 318, 322, 331, 372, 798, 819
LguI GCTCTTC 1 cut(s) 403
LmnI GCTCC 2 cut(s) 451, 807
Lsp1109I GCAGC 1 cut(s) 667
LweI GCATC 1 cut(s) 408
MaeI CTAG 4 cut(s) 59, 90, 158, 326
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 1 cut(s) 743
MalI GATC 7 cut(s) 305, 320, 324, 333, 374, 800, 821
MbiI CCGCTC 1 cut(s) 561
MboI GATC 7 cut(s) 303, 318, 322, 331, 372, 798, 819
MboII GAAGA 6 cut(s) 380, 420, 444, 507, 531, 623
MflI RGATCY 1 cut(s) 372
MhlI GDGCHC 2 cut(s) 415, 767
MluCI AATT 5 cut(s) 71, 117, 188, 283, 361
MluI ACGCGT 1 cut(s) 759
MlyI GAGTC 2 cut(s) 17, 401
MmeI TCCRAC 2 cut(s) 165, 188
Mph1103I ATGCAT 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 1 cut(s) 221
MspA1I CMGCKG 2 cut(s) 506, 580
MspI CCGG 8 cut(s) 545, 593, 644, 664, 679, 699, 749, 815
MspR9I CCNGG 3 cut(s) 644, 700, 749
MvnI CGCG 4 cut(s) 25, 506, 761, 803
MwoI GCNNNNNNNGC 3 cut(s) 506, 644, 675
NciI CCSGG 3 cut(s) 644, 700, 749
NdeI CATATG 1 cut(s) 105
NdeII GATC 7 cut(s) 303, 318, 322, 331, 372, 798, 819
NlaIII CATG 4 cut(s) 52, 172, 176, 181
NlaIV GGNNCC 3 cut(s) 79, 721, 737
NmeAIII GCCGAG 1 cut(s) 628
NotI GCGGCCGC 1 cut(s) 507
NruI TCGCGA 1 cut(s) 803
NsiI ATGCAT 1 cut(s) 174
NspI RCATGY 2 cut(s) 172, 176
NspV TTCGAA 1 cut(s) 359
PaeI GCATGC 1 cut(s) 172
PaeR7I CTCGAG 2 cut(s) 396, 836
PceI AGGCCT 1 cut(s) 464
PciSI GCTCTTC 1 cut(s) 403
PcsI WCGNNNNNNNCGW 4 cut(s) 327, 524, 527, 606
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 1 cut(s) 211
PinAI ACCGGT 1 cut(s) 663
PkrI GCNGC 4 cut(s) 46, 508, 511, 657
PleI GAGTC 2 cut(s) 16, 400
PpsI GAGTC 2 cut(s) 16, 400
PspN4I GGNNCC 3 cut(s) 79, 721, 737
PspPI GGNCC 3 cut(s) 78, 720, 736
PsuI RGATCY 1 cut(s) 372
RruI TCGCGA 1 cut(s) 803
RsaI GTAC 2 cut(s) 123, 274
RsaNI GTAC 2 cut(s) 122, 273
SacII CCGCGG 1 cut(s) 507
SapI GCTCTTC 1 cut(s) 403
SaqAI TTAA 1 cut(s) 221
SatI GCNGC 4 cut(s) 45, 507, 510, 656
Sau3AI GATC 7 cut(s) 303, 318, 322, 331, 372, 798, 819
Sau96I GGNCC 3 cut(s) 78, 720, 736
SchI GAGTC 2 cut(s) 17, 401
ScrFI CCNGG 3 cut(s) 644, 700, 749
SduI GDGCHC 2 cut(s) 415, 767
SetI ASST 7 cut(s) 60, 153, 600, 636, 675, 728, 837
SfaNI GCATC 1 cut(s) 408
SfcI CTRYAG 1 cut(s) 96
Sfr274I CTCGAG 2 cut(s) 396, 836
Sfr303I CCGCGG 1 cut(s) 507
SfuI TTCGAA 1 cut(s) 359
SgrBI CCGCGG 1 cut(s) 507
SinI GGWCC 3 cut(s) 78, 720, 736
SlaI CTCGAG 2 cut(s) 396, 836
SmlI CTYRAG 3 cut(s) 396, 446, 836
SmoI CTYRAG 3 cut(s) 396, 446, 836
SphI GCATGC 1 cut(s) 172
Sse9I AATT 5 cut(s) 71, 117, 188, 283, 361
SseBI AGGCCT 1 cut(s) 464
SspI AATATT 1 cut(s) 339
SspMI CTAG 4 cut(s) 59, 90, 158, 326
StuI AGGCCT 1 cut(s) 464
StyD4I CCNGG 3 cut(s) 642, 698, 747
TaaI ACNGT 3 cut(s) 481, 743, 784
TaiI ACGT 1 cut(s) 675
TaqI TCGA 6 cut(s) 321, 359, 397, 483, 771, 837
TasI AATT 5 cut(s) 71, 117, 188, 283, 361
TatI WGTACW 1 cut(s) 121
TauI GCSGC 3 cut(s) 47, 509, 512
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TseI GCWGC 1 cut(s) 655
TspDTI ATGAA 3 cut(s) 17, 25, 65
VpaK11BI GGWCC 3 cut(s) 78, 720, 736
XapI RAATTY 1 cut(s) 361
XceI RCATGY 2 cut(s) 172, 176
XhoI CTCGAG 2 cut(s) 396, 836
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 4 cut(s) 59, 90, 158, 326
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.