Rh4AG011900

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016411
Physical Location & Seq
Reverse (-)
1977159 .. 1986192
9034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG011900.1

Sequence Viewer

Length: 468 bp
ATGGGTTTGGGAAGCGAGGTGAAAGCGTCGTTGTTCATGAGCGATTTTCTGGAGAAGTGCGGTGGCTGTGCGGTTCTGGACGGCGGGTTTGCAACGGAGCTGGAACGACATGGAGCAGATCTCAACGACCCTCTCTGGAGCGCCAAATGCGTCGTCAGTTCTCCTCACCTCGTCGGAAGGGTACATTTGGACTACCTTGATGCTGGAGCAAACATCATAATAACCGCATCTTATCAGGCCACAATTCAGGGTTTTGAGGCCAAAGGTTTCTCCAAGGAAGAACCCAAAGCCCTGCTCAGGAAAAGTGTAGAAATTGCAATCGAGGCACGAGAAATTTACTATGACAAATGCACCAAAGGTTCTTGGGATTTTGTGGACACAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCAGCATCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGTATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

155

Amino Acids

16.71

Weight (kDa)

5.34

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 25 - 151 3.3e-29 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 60, 71, 84, 225
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 1 cut(s) 183
AfiI CCNNNNNNNGG 2 cut(s) 297, 462
AgsI TTSAA 1 cut(s) 393
AluBI AGCT 2 cut(s) 100, 429
AluI AGCT 2 cut(s) 100, 429
AlwNI CAGNNNCTG 1 cut(s) 419
AoxI GGCC 2 cut(s) 237, 258
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 1 cut(s) 333
ArsI GACNNNNNNTTYG 4 cut(s) 71, 103, 138, 170
Asp700I GAANNNNTTC 1 cut(s) 388
AspLEI GCGC 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 31, 158
BauI CACGAG 1 cut(s) 327
BbvI GCAGC 1 cut(s) 425
BccI CCATC 1 cut(s) 443
BceAI ACGGC 1 cut(s) 97
BfaI CTAG 1 cut(s) 407
BfoI RGCGCY 1 cut(s) 144
BglI GCCNNNNNGGC 1 cut(s) 443
BglII AGATCT 1 cut(s) 118
BisI GCNGC 1 cut(s) 414
BlsI GCNGC 1 cut(s) 415
BmiI GGNNCC 2 cut(s) 436, 454
BmsI GCATC 3 cut(s) 190, 236, 425
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 3 cut(s) 71, 157, 225
Bpu10I CCTNAGC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 273
Bsc4I CCNNNNNNNGG 2 cut(s) 297, 462
Bse1I ACTGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 2 cut(s) 297, 462
BseMII CTCAG 1 cut(s) 310
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 153
BseXI GCAGC 1 cut(s) 425
BshFI GGCC 2 cut(s) 239, 260
BslI CCNNNNNNNGG 2 cut(s) 297, 462
BsnI GGCC 2 cut(s) 239, 260
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 4 cut(s) 60, 71, 84, 225
BspANI GGCC 2 cut(s) 239, 260
BspCNI CTCAG 1 cut(s) 309
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 436, 454
BsrI ACTGG 1 cut(s) 401
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 1 cut(s) 118
BssSI CACGAG 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 273
Bst2BI CACGAG 1 cut(s) 327
BstDEI CTNAG 1 cut(s) 296
BstF5I GGATG 1 cut(s) 397
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 1 cut(s) 121
BstMBI GATC 1 cut(s) 118
BstMWI GCNNNNNNNGC 3 cut(s) 147, 323, 443
BstV1I GCAGC 1 cut(s) 425
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BsuRI GGCC 2 cut(s) 239, 260
BtsCI GGATG 1 cut(s) 397
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 143
CseI GACGC 2 cut(s) 15, 139
Csp6I GTAC 1 cut(s) 182
CviAII CATG 2 cut(s) 37, 110
CviJI RGCY 9 cut(s) 66, 100, 239, 260, 290, 388, 429, 437, 446
CviKI_1 RGCY 9 cut(s) 66, 100, 239, 260, 290, 388, 429, 437, 446
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 1 cut(s) 296
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 2 cut(s) 40, 113
FaiI YATR 6 cut(s) 38, 111, 218, 342, 432, 462
FatI CATG 2 cut(s) 36, 109
FauI CCCGC 1 cut(s) 77
Fnu4HI GCNGC 1 cut(s) 414
FokI GGATG 1 cut(s) 384
Fsp4HI GCNGC 1 cut(s) 414
FspBI CTAG 1 cut(s) 407
GlaI GCGC 1 cut(s) 142
GluI GCNGC 1 cut(s) 414
GsuI CTGGAG 3 cut(s) 71, 157, 225
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 2 cut(s) 239, 260
HgaI GACGC 2 cut(s) 15, 139
HhaI GCGC 1 cut(s) 143
Hin1II CATG 2 cut(s) 40, 113
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HphI GGTGA 2 cut(s) 31, 158
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 2 cut(s) 176, 457
Hpy188III TCNNGA 6 cut(s) 37, 50, 77, 136, 298, 393
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 3 cut(s) 31, 155, 176
HpyAV CCTTC 2 cut(s) 171, 399
HpyCH4V TGCA 4 cut(s) 92, 317, 351, 413
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 323, 443
HpyF3I CTNAG 1 cut(s) 296
Hsp92II CATG 2 cut(s) 40, 113
HspAI GCGC 1 cut(s) 141
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 5 cut(s) 97, 113, 138, 206, 434
Lsp1109I GCAGC 1 cut(s) 425
LweI GCATC 3 cut(s) 190, 236, 425
MaeI CTAG 1 cut(s) 407
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 1 cut(s) 290
MflI RGATCY 1 cut(s) 118
MluCI AATT 3 cut(s) 243, 312, 333
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 6 cut(s) 10, 141, 174, 179, 250, 316
MroXI GAANNNNTTC 1 cut(s) 388
MwoI GCNNNNNNNGC 3 cut(s) 147, 323, 443
NdeII GATC 1 cut(s) 118
NlaIII CATG 2 cut(s) 40, 113
NlaIV GGNNCC 2 cut(s) 436, 454
PagI TCATGA 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 388
PkrI GCNGC 1 cut(s) 415
PspN4I GGNNCC 2 cut(s) 436, 454
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 118
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SatI GCNGC 1 cut(s) 414
Sau3AI GATC 1 cut(s) 118
SetI ASST 8 cut(s) 21, 102, 171, 198, 268, 361, 431, 467
SfaNI GCATC 3 cut(s) 190, 236, 425
Sse9I AATT 3 cut(s) 243, 312, 333
SsiI CCGC 4 cut(s) 60, 71, 84, 225
SspMI CTAG 1 cut(s) 407
StyI CCWWGG 1 cut(s) 273
TaqI TCGA 1 cut(s) 321
TasI AATT 3 cut(s) 243, 312, 333
TseI GCWGC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 25
TspGWI ACGGA 1 cut(s) 110
XapI RAATTY 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 388
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.