Rh4CG016500

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016411
Physical Location & Seq
Reverse (-)
3156240 .. 3177239
21000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG016500.1

Sequence Viewer

Length: 525 bp
ATGGATTTGGGAAGCGAGAAAACGTCGTCGTTCATGAGCGATTTTCTGGAAAAGTGCGGTGGCTGTGCCGTTCTGGACGGCGGGTTTGCGACGGAGCTGGAACGACATGGAGCTGATCTCAACGACCCTCTCTGGAGCGCCAAATGCCTCGTCAGTTCTCCTCACCTCGTCCGTAGGGTACATTTGGACTACCTTGATGCTGGAGCAAACATCATAATAACCGCATCTTATCAGGCCACAATTCAGGGTTTTGAGGCCAAAGGTTTCTCTAAAGAAGAAGCCAAAGCCCTGCTCAGGAAAAGTGTAGAAATTGCAATCGAGGCACGAGAAATTTACTATGACAAATGCACCAAAGGTTCTTGGGATTTTGTGGACACAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCAGCATCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGTATAGTCTCAGACTACTAGGACTAGCACAAACAAATAACTCGAGATTAAAAGGAGAACGAGAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

174

Amino Acids

18.94

Weight (kDa)

5.59

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 24 - 154 1.6e-31 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 57, 81, 222
AcsI RAATTY 1 cut(s) 330
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 294
AgsI TTSAA 1 cut(s) 390
AluBI AGCT 3 cut(s) 97, 113, 426
AluI AGCT 3 cut(s) 97, 113, 426
Alw26I GTCTC 2 cut(s) 467, 512
AlwNI CAGNNNCTG 1 cut(s) 416
Ama87I CYCGRG 1 cut(s) 496
AoxI GGCC 2 cut(s) 234, 255
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 1 cut(s) 330
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 135, 167
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 496
BauI CACGAG 1 cut(s) 324
BbvI GCAGC 1 cut(s) 422
BccI CCATC 1 cut(s) 440
BceAI ACGGC 2 cut(s) 53, 94
BcoDI GTCTC 2 cut(s) 467, 512
BfaI CTAG 3 cut(s) 404, 473, 479
BfoI RGCGCY 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 1 cut(s) 411
BlsI GCNGC 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 496
BmiI GGNNCC 2 cut(s) 433, 451
BmsI GCATC 3 cut(s) 187, 233, 422
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BpmI CTGGAG 2 cut(s) 154, 222
Bpu10I CCTNAGC 1 cut(s) 293
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse1I ACTGG 1 cut(s) 398
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 2 cut(s) 307, 478
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 422
BshFI GGCC 2 cut(s) 236, 257
BsiHKCI CYCGRG 1 cut(s) 496
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 2 cut(s) 467, 512
BsnI GGCC 2 cut(s) 236, 257
BsoBI CYCGRG 1 cut(s) 496
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 3 cut(s) 57, 81, 222
BspANI GGCC 2 cut(s) 236, 257
BspCNI CTCAG 2 cut(s) 306, 477
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 433, 451
BsrI ACTGG 1 cut(s) 398
BssMI GATC 1 cut(s) 115
BssSI CACGAG 1 cut(s) 324
Bst2BI CACGAG 1 cut(s) 324
BstDEI CTNAG 2 cut(s) 293, 464
BstF5I GGATG 1 cut(s) 394
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 2 cut(s) 467, 512
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 3 cut(s) 144, 320, 440
BstV1I GCAGC 1 cut(s) 422
BsuRI GGCC 2 cut(s) 236, 257
BtsCI GGATG 1 cut(s) 394
CaiI CAGNNNCTG 1 cut(s) 416
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 140
Csp6I GTAC 1 cut(s) 179
CviAII CATG 2 cut(s) 34, 107
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 2 cut(s) 293, 464
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
Eco88I CYCGRG 1 cut(s) 496
FaeI CATG 2 cut(s) 37, 110
FaiI YATR 6 cut(s) 35, 108, 215, 339, 429, 459
FatI CATG 2 cut(s) 33, 106
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 1 cut(s) 411
FokI GGATG 1 cut(s) 381
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 3 cut(s) 404, 473, 479
GlaI GCGC 1 cut(s) 139
GluI GCNGC 1 cut(s) 411
GsuI CTGGAG 2 cut(s) 154, 222
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 2 cut(s) 236, 257
HhaI GCGC 1 cut(s) 140
Hin1II CATG 2 cut(s) 37, 110
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 454, 467
Hpy188III TCNNGA 7 cut(s) 34, 47, 74, 133, 295, 390, 498
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 3 cut(s) 28, 31, 94
HpyAV CCTTC 1 cut(s) 396
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 314, 348, 410
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 320, 440
HpyF3I CTNAG 2 cut(s) 293, 464
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 2 cut(s) 37, 110
HspAI GCGC 1 cut(s) 138
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 5 cut(s) 94, 110, 135, 203, 431
Lsp1109I GCAGC 1 cut(s) 422
LweI GCATC 3 cut(s) 187, 233, 422
MaeI CTAG 3 cut(s) 404, 473, 479
MaeII ACGT 1 cut(s) 23
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 1 cut(s) 287
MluCI AATT 3 cut(s) 240, 309, 330
MnlI CCTC 6 cut(s) 138, 158, 171, 176, 247, 313
MroXI GAANNNNTTC 1 cut(s) 385
MseI TTAA 2 cut(s) 503, 523
MwoI GCNNNNNNNGC 3 cut(s) 144, 320, 440
NdeII GATC 1 cut(s) 115
NlaIII CATG 2 cut(s) 37, 110
NlaIV GGNNCC 2 cut(s) 433, 451
PaeR7I CTCGAG 1 cut(s) 496
PagI TCATGA 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 385
PkrI GCNGC 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 433, 451
PstNI CAGNNNCTG 1 cut(s) 416
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
SaqAI TTAA 2 cut(s) 503, 523
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 1 cut(s) 115
SetI ASST 8 cut(s) 26, 99, 115, 168, 195, 265, 358, 428
SfaNI GCATC 3 cut(s) 187, 233, 422
Sfr274I CTCGAG 1 cut(s) 496
SlaI CTCGAG 1 cut(s) 496
SmlI CTYRAG 1 cut(s) 496
SmoI CTYRAG 1 cut(s) 496
Sse9I AATT 3 cut(s) 240, 309, 330
SsiI CCGC 3 cut(s) 57, 81, 222
SspMI CTAG 3 cut(s) 404, 473, 479
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 318, 497
TasI AATT 3 cut(s) 240, 309, 330
Tru1I TTAA 2 cut(s) 503, 523
Tru9I TTAA 2 cut(s) 503, 523
TseI GCWGC 1 cut(s) 410
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 2 cut(s) 107, 161
XapI RAATTY 1 cut(s) 330
XhoI CTCGAG 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 385
XspI CTAG 3 cut(s) 404, 473, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.