Rh4AG015300

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016411
Physical Location & Seq
Reverse (-)
3111994 .. 3113310
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG015300.1

Sequence Viewer

Length: 489 bp
ATGGGTTTGGGAAGCGAGGTGAAAACATCGTCGTTCATGAGCGATTTTCTGGAGAAGTGCGGTGGCTGTGCCGTTCTGGACGGCGGGTTTGGGACGGAGCTGGAACGACATGGAGCTGATATCAACGACCCTCTCTGGAGCGCCAAATGCCTCGTCAGTTCTCCTCACCTCGTCCGTAGGGTACATTTGGACTACCTTGATGCTGGCGCAAGCATCATAATAACCGCATCTTATCAGGCCACAATTCAGGGTTTTGAGGCCAAAGGCTTCTCTAAAGAAGAAGCCAAAGCCCTGCTCAGGAAAAGTTTAGAAATTGCAATTGAGGCACAAGAAATTTACTATGACAAATGCACCAAAGGTTCTTGGGATTTTGTGGACGCAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCAGCATCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGAATAGGTCAGTTAACTCAGCATATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

162

Amino Acids

17.41

Weight (kDa)

5.2

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 25 - 153 1.5e-30 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 60, 84, 225
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 1 cut(s) 183
AfiI CCNNNNNNNGG 2 cut(s) 297, 462
AgsI TTSAA 1 cut(s) 393
AluBI AGCT 3 cut(s) 100, 116, 429
AluI AGCT 3 cut(s) 100, 116, 429
AlwNI CAGNNNCTG 1 cut(s) 419
AoxI GGCC 2 cut(s) 237, 258
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 1 cut(s) 333
ArsI GACNNNNNNTTYG 4 cut(s) 71, 103, 138, 170
Asp700I GAANNNNTTC 1 cut(s) 388
AspLEI GCGC 2 cut(s) 143, 209
AsuHPI GGTGA 2 cut(s) 31, 158
BbvI GCAGC 1 cut(s) 425
BccI CCATC 1 cut(s) 443
BceAI ACGGC 2 cut(s) 56, 97
BfaI CTAG 1 cut(s) 407
BfoI RGCGCY 1 cut(s) 144
BglI GCCNNNNNGGC 1 cut(s) 443
BisI GCNGC 1 cut(s) 414
BlsI GCNGC 1 cut(s) 415
BmiI GGNNCC 2 cut(s) 436, 454
BmsI GCATC 4 cut(s) 190, 222, 236, 425
BpmI CTGGAG 2 cut(s) 71, 157
Bpu10I CCTNAGC 1 cut(s) 296
Bsc4I CCNNNNNNNGG 2 cut(s) 297, 462
Bse1I ACTGG 1 cut(s) 401
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 2 cut(s) 297, 462
BseMII CTCAG 2 cut(s) 310, 489
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 153
BseXI GCAGC 1 cut(s) 425
BshFI GGCC 2 cut(s) 239, 260
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 2 cut(s) 297, 462
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 2 cut(s) 239, 260
BspACI CCGC 3 cut(s) 60, 84, 225
BspANI GGCC 2 cut(s) 239, 260
BspCNI CTCAG 2 cut(s) 309, 488
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 436, 454
BsrI ACTGG 1 cut(s) 401
BstC8I GCNNGC 2 cut(s) 205, 211
BstDEI CTNAG 2 cut(s) 296, 475
BstF5I GGATG 1 cut(s) 397
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 2 cut(s) 143, 209
BstMWI GCNNNNNNNGC 3 cut(s) 147, 323, 443
BstV1I GCAGC 1 cut(s) 425
BsuRI GGCC 2 cut(s) 239, 260
BtsCI GGATG 1 cut(s) 397
Cac8I GCNNGC 2 cut(s) 205, 211
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 36
CfoI GCGC 2 cut(s) 143, 209
CseI GACGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 182
CviAII CATG 2 cut(s) 37, 110
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 2 cut(s) 296, 475
Eco32I GATATC 1 cut(s) 121
EcoRV GATATC 1 cut(s) 121
FaeI CATG 2 cut(s) 40, 113
FaiI YATR 7 cut(s) 38, 111, 218, 342, 432, 481, 483
FaqI GGGAC 1 cut(s) 106
FatI CATG 2 cut(s) 36, 109
FauI CCCGC 1 cut(s) 77
FauNDI CATATG 1 cut(s) 481
Fnu4HI GCNGC 1 cut(s) 414
FokI GGATG 1 cut(s) 384
Fsp4HI GCNGC 1 cut(s) 414
FspBI CTAG 1 cut(s) 407
GlaI GCGC 2 cut(s) 142, 208
GluI GCNGC 1 cut(s) 414
GsuI CTGGAG 2 cut(s) 71, 157
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 2 cut(s) 239, 260
HgaI GACGC 1 cut(s) 386
HhaI GCGC 2 cut(s) 143, 209
Hin1II CATG 2 cut(s) 40, 113
Hin6I GCGC 2 cut(s) 141, 207
HinP1I GCGC 2 cut(s) 141, 207
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HpaI GTTAAC 1 cut(s) 472
HphI GGTGA 2 cut(s) 31, 158
Hpy166II GTNNAC 2 cut(s) 376, 472
Hpy188I TCNGA 1 cut(s) 457
Hpy188III TCNNGA 6 cut(s) 37, 50, 77, 136, 298, 393
Hpy8I GTNNAC 2 cut(s) 376, 472
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 399
HpyCH4V TGCA 3 cut(s) 317, 351, 413
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 323, 443
HpyF3I CTNAG 2 cut(s) 296, 475
Hsp92II CATG 2 cut(s) 40, 113
HspAI GCGC 2 cut(s) 141, 207
KspAI GTTAAC 1 cut(s) 472
LmnI GCTCC 4 cut(s) 97, 113, 138, 434
Lsp1109I GCAGC 1 cut(s) 425
LweI GCATC 4 cut(s) 190, 222, 236, 425
MaeI CTAG 1 cut(s) 407
MboII GAAGA 1 cut(s) 290
MfeI CAATTG 1 cut(s) 318
MluCI AATT 4 cut(s) 243, 312, 318, 333
MnlI CCTC 7 cut(s) 10, 141, 161, 174, 179, 250, 316
MroXI GAANNNNTTC 1 cut(s) 388
MseI TTAA 1 cut(s) 471
MunI CAATTG 1 cut(s) 318
MwoI GCNNNNNNNGC 3 cut(s) 147, 323, 443
NdeI CATATG 1 cut(s) 481
NlaIII CATG 2 cut(s) 40, 113
NlaIV GGNNCC 2 cut(s) 436, 454
PagI TCATGA 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 388
PkrI GCNGC 1 cut(s) 415
PspN4I GGNNCC 2 cut(s) 436, 454
PstNI CAGNNNCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 1 cut(s) 414
SetI ASST 8 cut(s) 21, 102, 118, 171, 198, 361, 431, 467
SfaNI GCATC 4 cut(s) 190, 222, 236, 425
Sse9I AATT 4 cut(s) 243, 312, 318, 333
SsiI CCGC 3 cut(s) 60, 84, 225
SspMI CTAG 1 cut(s) 407
TasI AATT 4 cut(s) 243, 312, 318, 333
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TseI GCWGC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 25
TspGWI ACGGA 2 cut(s) 110, 164
XapI RAATTY 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 388
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.