Rh4BG010300

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016411
Physical Location & Seq
Reverse (-)
1492528 .. 1493810
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG010300.1

Sequence Viewer

Length: 465 bp
ATGGATTTGGGAAGCGAGAAAACGTCGTCGTTCATGAGCGATTTTCTGGAAAAGTGCGGTGGCTGTGCCGTTCTGGACGGCGGGTTTGCGACGGAGCTGGAACGACATGGAGCTGATCTCAACGACCCTCTCTGGAGCGCCAAATGCCTCGTCAGTTCTCCTCACCTCGTCCGTAGGGTACATTTGGACTACCTTGATGCTGGAGCAAACATCATAATAACCGCATCTTATCAGGCCACAATTCAGGGTTTTGAGGCCAAAGGTTTCTCTAAAGAAGAAGCCAAAGCCCTGCTCAGGAAAAGTGTAGAAATTGCAATTGAGGCACGAGAAATTTACTATGATAAATGCACCAAAGGGTCTTGGGATTTTGTGGACGCAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCAGCATCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGTATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

154

Amino Acids

16.73

Weight (kDa)

5.35

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 24 - 150 3.3e-31 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 57, 81, 222
AcsI RAATTY 1 cut(s) 330
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 2 cut(s) 294, 459
AgsI TTSAA 1 cut(s) 390
AluBI AGCT 3 cut(s) 97, 113, 426
AluI AGCT 3 cut(s) 97, 113, 426
AlwNI CAGNNNCTG 1 cut(s) 416
AoxI GGCC 2 cut(s) 234, 255
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 1 cut(s) 330
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 135, 167
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 155
BauI CACGAG 1 cut(s) 324
BbvI GCAGC 1 cut(s) 422
BccI CCATC 1 cut(s) 440
BceAI ACGGC 2 cut(s) 53, 94
BfaI CTAG 1 cut(s) 404
BfoI RGCGCY 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 1 cut(s) 411
BlsI GCNGC 1 cut(s) 412
BmiI GGNNCC 2 cut(s) 433, 451
BmsI GCATC 3 cut(s) 187, 233, 422
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BpmI CTGGAG 2 cut(s) 154, 222
Bpu10I CCTNAGC 1 cut(s) 293
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 459
Bse1I ACTGG 1 cut(s) 398
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 2 cut(s) 294, 459
BseMII CTCAG 1 cut(s) 307
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 422
BshFI GGCC 2 cut(s) 236, 257
BslI CCNNNNNNNGG 2 cut(s) 294, 459
BsnI GGCC 2 cut(s) 236, 257
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 3 cut(s) 57, 81, 222
BspANI GGCC 2 cut(s) 236, 257
BspCNI CTCAG 1 cut(s) 306
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 433, 451
BsrI ACTGG 1 cut(s) 398
BssMI GATC 1 cut(s) 115
BssSI CACGAG 1 cut(s) 324
Bst2BI CACGAG 1 cut(s) 324
BstDEI CTNAG 1 cut(s) 293
BstF5I GGATG 1 cut(s) 394
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 1 cut(s) 118
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 3 cut(s) 144, 320, 440
BstV1I GCAGC 1 cut(s) 422
BsuRI GGCC 2 cut(s) 236, 257
BtsCI GGATG 1 cut(s) 394
CaiI CAGNNNCTG 1 cut(s) 416
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 140
CseI GACGC 1 cut(s) 383
Csp6I GTAC 1 cut(s) 179
CviAII CATG 2 cut(s) 34, 107
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 1 cut(s) 293
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
FaeI CATG 2 cut(s) 37, 110
FaiI YATR 6 cut(s) 35, 108, 215, 339, 429, 459
FatI CATG 2 cut(s) 33, 106
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 1 cut(s) 411
FokI GGATG 1 cut(s) 381
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 139
GluI GCNGC 1 cut(s) 411
GsuI CTGGAG 2 cut(s) 154, 222
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 2 cut(s) 236, 257
HgaI GACGC 1 cut(s) 383
HhaI GCGC 1 cut(s) 140
Hin1II CATG 2 cut(s) 37, 110
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 1 cut(s) 454
Hpy188III TCNNGA 6 cut(s) 34, 47, 74, 133, 295, 390
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 3 cut(s) 28, 31, 94
HpyAV CCTTC 1 cut(s) 396
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 314, 348, 410
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 320, 440
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 2 cut(s) 37, 110
HspAI GCGC 1 cut(s) 138
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 5 cut(s) 94, 110, 135, 203, 431
Lsp1109I GCAGC 1 cut(s) 422
LweI GCATC 3 cut(s) 187, 233, 422
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 23
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 1 cut(s) 287
MfeI CAATTG 1 cut(s) 315
MluCI AATT 4 cut(s) 240, 309, 315, 330
MnlI CCTC 6 cut(s) 138, 158, 171, 176, 247, 313
MroXI GAANNNNTTC 1 cut(s) 385
MunI CAATTG 1 cut(s) 315
MwoI GCNNNNNNNGC 3 cut(s) 144, 320, 440
NdeII GATC 1 cut(s) 115
NlaIII CATG 2 cut(s) 37, 110
NlaIV GGNNCC 2 cut(s) 433, 451
PagI TCATGA 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 385
PkrI GCNGC 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 433, 451
PstNI CAGNNNCTG 1 cut(s) 416
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 1 cut(s) 115
SetI ASST 8 cut(s) 26, 99, 115, 168, 195, 265, 428, 464
SfaNI GCATC 3 cut(s) 187, 233, 422
Sse9I AATT 4 cut(s) 240, 309, 315, 330
SsiI CCGC 3 cut(s) 57, 81, 222
SspMI CTAG 1 cut(s) 404
TaiI ACGT 1 cut(s) 26
TasI AATT 4 cut(s) 240, 309, 315, 330
TseI GCWGC 1 cut(s) 410
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 2 cut(s) 107, 161
XapI RAATTY 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 385
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.