Rh4CG016400

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016411
Physical Location & Seq
Reverse (-)
3152486 .. 3153821
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG016400.1

Sequence Viewer

Length: 468 bp
ATGGGTTTGGGAAGCGAGGTGAAAACGTTGTCGTTCATGAGCGATTTTCTGGAGAAGTGCGGTGGCTGTGCCCTTCTGGACGGCGGGTTTGCGACGGAGCTGGAACGGCATGGAGCTGATCTCAACGATCCTCTCAGGAGCGCCAAATGCCTCGTCAGTTCTCCTCACCTCGTCCGAAGGGTACATTTGGACTACCTTGATGCTGGAGCAAACATCATAATAACCGCATCTTATCAGGCCACAATTCAGGGTTTTGAGGCCAAAGGTTTCTCTAAAGAAGAAGCCAAAGCCCTGCTCAGGAAAAGTGTAGAAATTGCAATTGAGGCACGAGAAATTTACTATGACAAATGCACCAAAGGTTCTTGGGATTTTGTGGACGCAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCAGCATCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGTATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

155

Amino Acids

16.78

Weight (kDa)

5.8

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 25 - 151 4.8e-30 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 60, 84, 225
AclI AACGTT 1 cut(s) 26
AclWI GGATC 1 cut(s) 122
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 1 cut(s) 183
AfiI CCNNNNNNNGG 2 cut(s) 297, 462
AgsI TTSAA 1 cut(s) 393
AluBI AGCT 3 cut(s) 100, 116, 429
AluI AGCT 3 cut(s) 100, 116, 429
AlwI GGATC 1 cut(s) 122
AlwNI CAGNNNCTG 1 cut(s) 419
AoxI GGCC 2 cut(s) 237, 258
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 1 cut(s) 333
ArsI GACNNNNNNTTYG 4 cut(s) 71, 103, 138, 170
Asp700I GAANNNNTTC 1 cut(s) 388
AspLEI GCGC 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 31, 158
BaeGI GKGCMC 1 cut(s) 73
BauI CACGAG 1 cut(s) 327
BbvI GCAGC 1 cut(s) 425
BccI CCATC 1 cut(s) 443
BceAI ACGGC 2 cut(s) 97, 122
BfaI CTAG 1 cut(s) 407
BfoI RGCGCY 1 cut(s) 144
BglI GCCNNNNNGGC 1 cut(s) 443
BisI GCNGC 1 cut(s) 414
BlsI GCNGC 1 cut(s) 415
BmiI GGNNCC 2 cut(s) 436, 454
BmsI GCATC 3 cut(s) 190, 236, 425
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 2 cut(s) 71, 225
Bpu10I CCTNAGC 1 cut(s) 296
Bsc4I CCNNNNNNNGG 2 cut(s) 297, 462
Bse1I ACTGG 1 cut(s) 401
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 2 cut(s) 297, 462
BseMII CTCAG 2 cut(s) 148, 310
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 153
BseSI GKGCMC 1 cut(s) 73
BseXI GCAGC 1 cut(s) 425
BshFI GGCC 2 cut(s) 239, 260
BslI CCNNNNNNNGG 2 cut(s) 297, 462
BsnI GGCC 2 cut(s) 239, 260
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 2 cut(s) 118, 127
BspACI CCGC 3 cut(s) 60, 84, 225
BspANI GGCC 2 cut(s) 239, 260
BspCNI CTCAG 2 cut(s) 147, 309
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 436, 454
BspPI GGATC 1 cut(s) 122
BsrI ACTGG 1 cut(s) 401
BssMI GATC 2 cut(s) 118, 127
BssSI CACGAG 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 327
BstDEI CTNAG 2 cut(s) 134, 296
BstF5I GGATG 1 cut(s) 397
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 2 cut(s) 121, 130
BstMBI GATC 2 cut(s) 118, 127
BstMWI GCNNNNNNNGC 4 cut(s) 106, 147, 323, 443
BstSLI GKGCMC 1 cut(s) 73
BstV1I GCAGC 1 cut(s) 425
BsuRI GGCC 2 cut(s) 239, 260
BtsCI GGATG 1 cut(s) 397
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 143
CseI GACGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 182
CviAII CATG 2 cut(s) 37, 110
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 2 cut(s) 134, 296
DpnI GATC 2 cut(s) 120, 129
DpnII GATC 2 cut(s) 118, 127
FaeI CATG 2 cut(s) 40, 113
FaiI YATR 6 cut(s) 38, 111, 218, 342, 432, 462
FatI CATG 2 cut(s) 36, 109
FauI CCCGC 1 cut(s) 77
Fnu4HI GCNGC 1 cut(s) 414
FokI GGATG 1 cut(s) 384
Fsp4HI GCNGC 1 cut(s) 414
FspBI CTAG 1 cut(s) 407
GlaI GCGC 1 cut(s) 142
GluI GCNGC 1 cut(s) 414
GsuI CTGGAG 2 cut(s) 71, 225
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 2 cut(s) 239, 260
HgaI GACGC 1 cut(s) 386
HhaI GCGC 1 cut(s) 143
Hin1II CATG 2 cut(s) 40, 113
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HphI GGTGA 2 cut(s) 31, 158
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 2 cut(s) 176, 457
Hpy188III TCNNGA 6 cut(s) 37, 50, 77, 136, 298, 393
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 3 cut(s) 83, 171, 399
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 3 cut(s) 317, 351, 413
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 147, 323, 443
HpyF3I CTNAG 2 cut(s) 134, 296
HpySE526I ACGT 1 cut(s) 26
Hsp92II CATG 2 cut(s) 40, 113
HspAI GCGC 1 cut(s) 141
Kzo9I GATC 2 cut(s) 118, 127
LmnI GCTCC 5 cut(s) 97, 113, 138, 206, 434
Lsp1109I GCAGC 1 cut(s) 425
LweI GCATC 3 cut(s) 190, 236, 425
MaeI CTAG 1 cut(s) 407
MaeII ACGT 1 cut(s) 26
MalI GATC 2 cut(s) 120, 129
MboI GATC 2 cut(s) 118, 127
MboII GAAGA 1 cut(s) 290
MfeI CAATTG 1 cut(s) 318
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 4 cut(s) 243, 312, 318, 333
MnlI CCTC 7 cut(s) 10, 141, 161, 174, 179, 250, 316
MroXI GAANNNNTTC 1 cut(s) 388
MunI CAATTG 1 cut(s) 318
MwoI GCNNNNNNNGC 4 cut(s) 106, 147, 323, 443
NdeII GATC 2 cut(s) 118, 127
NlaIII CATG 2 cut(s) 40, 113
NlaIV GGNNCC 2 cut(s) 436, 454
PagI TCATGA 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 388
PkrI GCNGC 1 cut(s) 415
Psp1406I AACGTT 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 436, 454
PstNI CAGNNNCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SatI GCNGC 1 cut(s) 414
Sau3AI GATC 2 cut(s) 118, 127
SduI GDGCHC 1 cut(s) 73
SfaNI GCATC 3 cut(s) 190, 236, 425
Sse9I AATT 4 cut(s) 243, 312, 318, 333
SsiI CCGC 3 cut(s) 60, 84, 225
SspMI CTAG 1 cut(s) 407
TaiI ACGT 1 cut(s) 29
TasI AATT 4 cut(s) 243, 312, 318, 333
TseI GCWGC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 25
TspGWI ACGGA 1 cut(s) 110
XapI RAATTY 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 388
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.