Rh4AG073300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013768
Physical Location & Seq
Forward (+)
15070374 .. 15080953
10580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG073300.1

Sequence Viewer

Length: 441 bp
ATGCTAGCTAGGCCTTTGGTGCTTTGCAAAGGGTTTGGCTGTCAAGGCTGTGTCTGCGAATATGTTCCTCTTCCGTTTTGTTTTGGAATCGGACCTGGAATTTGTGTTAGCCAGGGAACTGTGGAACTTTGTGGGAAACCCACTACTTGTGAAAAGATTGCCCTCTTAGTTGAGTTCCATCCAAAGAATGCAGAAGATTTCTTATTCACAAAGAAGCTTAAAGAAGTTGAACGCATCTCACCACCACCGTGTCACCCACGGTCTACTCTGGTGAGAAGTACTGCTCGTGTCTTACCAGATTCTGCCTTAAATGCTAGTATTTCACATGTGCTCGCGAAGACGGTTGGTCAGCAAGCAGTTACCAATTCTAAAAAGGAGGACAGGTTTCGGTATCTAGTTAAGGACAGCTATCCAAGTCTTTTCGAGCCGGTCTGTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

15.97

Weight (kDa)

8.7

Isoelectric Point (pI)

30.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 263
AccII CGCG 1 cut(s) 335
AcsI RAATTY 1 cut(s) 99
AfaI GTAC 1 cut(s) 280
AfiI CCNNNNNNNGG 1 cut(s) 434
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 1 cut(s) 230
AhdI GACNNNNNGTC 1 cut(s) 345
AjnI CCWGG 2 cut(s) 94, 111
AleI CACNNNNGTG 1 cut(s) 247
AluBI AGCT 3 cut(s) 8, 217, 408
AluI AGCT 3 cut(s) 8, 217, 408
Alw21I GWGCWC 1 cut(s) 333
AlwNI CAGNNNCTG 1 cut(s) 302
AoxI GGCC 1 cut(s) 11
ApoI RAATTY 1 cut(s) 99
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
Asp700I GAANNNNTTC 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 92
AsuHPI GGTGA 3 cut(s) 231, 245, 283
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 92
BauI CACGAG 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 333
BccI CCATC 1 cut(s) 186
BciT130I CCWGG 2 cut(s) 96, 113
BfaI CTAG 4 cut(s) 5, 9, 315, 395
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 2 cut(s) 96, 113
Bme18I GGWCC 1 cut(s) 92
BmeRI GACNNNNNGTC 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 92
BmrFI CCNGG 2 cut(s) 96, 113
BmsI GCATC 1 cut(s) 243
BmtI GCTAGC 1 cut(s) 8
BpiI GAAGAC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 112, 257
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse118I RCCGGY 1 cut(s) 427
BseBI CCWGG 2 cut(s) 96, 113
BseDI CCNNGG 2 cut(s) 112, 257
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 434
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 1 cut(s) 428
BslI CCNNNNNNNGG 1 cut(s) 434
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 333
Bsp68I TCGCGA 1 cut(s) 335
BspANI GGCC 1 cut(s) 13
BspFNI CGCG 1 cut(s) 335
BspOI GCTAGC 1 cut(s) 8
BsrFI RCCGGY 1 cut(s) 427
BssAI RCCGGY 1 cut(s) 427
BssECI CCNNGG 2 cut(s) 112, 257
BssSI CACGAG 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 285
Bst2UI CCWGG 2 cut(s) 96, 113
Bst4CI ACNGT 4 cut(s) 121, 249, 261, 343
Bst6I CTCTTC 1 cut(s) 75
BstC8I GCNNGC 3 cut(s) 6, 333, 354
BstDEI CTNAG 1 cut(s) 166
BstDSI CCRYGG 1 cut(s) 257
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 335
BstMWI GCNNNNNNNGC 5 cut(s) 10, 19, 45, 54, 311
BstNI CCWGG 2 cut(s) 96, 113
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 94, 111
BstUI CGCG 1 cut(s) 335
BstV2I GAAGAC 1 cut(s) 344
BsuRI GGCC 1 cut(s) 13
BtgI CCRYGG 1 cut(s) 257
BtsCI GGATG 1 cut(s) 178
BtuMI TCGCGA 1 cut(s) 335
Cac8I GCNNGC 3 cut(s) 6, 333, 354
CaiI CAGNNNCTG 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 427
Cfr13I GGNCC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 279
CviAII CATG 1 cut(s) 326
CviJI RGCY 8 cut(s) 8, 13, 39, 48, 111, 217, 408, 427
CviKI_1 RGCY 8 cut(s) 8, 13, 39, 48, 111, 217, 408, 427
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 1 cut(s) 166
DriI GACNNNNNGTC 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 75
Eam1105I GACNNNNNGTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 75
Eco147I AGGCCT 1 cut(s) 13
Eco47I GGWCC 1 cut(s) 92
EcoRII CCWGG 2 cut(s) 94, 111
FaeI CATG 1 cut(s) 329
FaiI YATR 2 cut(s) 63, 327
FatI CATG 1 cut(s) 325
FblI GTMKAC 1 cut(s) 263
FokI GGATG 1 cut(s) 165
FspBI CTAG 4 cut(s) 5, 9, 315, 395
HaeIII GGCC 1 cut(s) 13
HapII CCGG 1 cut(s) 428
Hin1II CATG 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 2 cut(s) 87, 299
HpaII CCGG 1 cut(s) 428
HphI GGTGA 3 cut(s) 231, 245, 283
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 1 cut(s) 92
Hpy188III TCNNGA 1 cut(s) 334
Hpy8I GTNNAC 1 cut(s) 264
HpyCH4III ACNGT 4 cut(s) 121, 249, 261, 343
HpyCH4V TGCA 2 cut(s) 27, 191
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 19, 45, 54, 311
HpyF3I CTNAG 1 cut(s) 166
Hsp92II CATG 1 cut(s) 329
LpnPI CCDG 7 cut(s) 81, 98, 108, 125, 254, 309, 367
LweI GCATC 1 cut(s) 243
MaeI CTAG 4 cut(s) 5, 9, 315, 395
MaeIII GTNAC 2 cut(s) 251, 358
MboII GAAGA 3 cut(s) 62, 206, 349
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 2 cut(s) 99, 364
MnlI CCTC 3 cut(s) 78, 173, 370
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 3 cut(s) 219, 308, 399
MslI CAYNNNNRTG 1 cut(s) 247
MspI CCGG 1 cut(s) 428
MspR9I CCNGG 2 cut(s) 96, 113
Mva1269I GAATGC 1 cut(s) 193
MvaI CCWGG 2 cut(s) 96, 113
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 5 cut(s) 10, 19, 45, 54, 311
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 1 cut(s) 329
NmuCI GTSAC 1 cut(s) 251
NruI TCGCGA 1 cut(s) 335
NspI RCATGY 1 cut(s) 329
OliI CACNNNNGTG 1 cut(s) 247
PceI AGGCCT 1 cut(s) 13
PciI ACATGT 1 cut(s) 325
PctI GAATGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 87, 299
PscI ACATGT 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 94, 111
PspGI CCWGG 2 cut(s) 94, 111
PspPI GGNCC 1 cut(s) 92
PstNI CAGNNNCTG 1 cut(s) 302
RruI TCGCGA 1 cut(s) 335
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
RseI CAYNNNNRTG 1 cut(s) 247
SaqAI TTAA 3 cut(s) 219, 308, 399
Sau96I GGNCC 1 cut(s) 92
ScaI AGTACT 1 cut(s) 280
ScrFI CCNGG 2 cut(s) 96, 113
SduI GDGCHC 1 cut(s) 333
SetI ASST 5 cut(s) 10, 97, 219, 386, 410
SfaNI GCATC 1 cut(s) 243
SinI GGWCC 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 247
Sse9I AATT 2 cut(s) 99, 364
SseBI AGGCCT 1 cut(s) 13
SspMI CTAG 4 cut(s) 5, 9, 315, 395
StuI AGGCCT 1 cut(s) 13
StyD4I CCNGG 2 cut(s) 94, 111
TaaI ACNGT 4 cut(s) 121, 249, 261, 343
TaqI TCGA 1 cut(s) 423
TasI AATT 2 cut(s) 99, 364
TatI WGTACW 1 cut(s) 278
TfiI GAWTC 2 cut(s) 87, 299
Tru1I TTAA 3 cut(s) 219, 308, 399
Tru9I TTAA 3 cut(s) 219, 308, 399
TseFI GTSAC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 251
TspGWI ACGGA 1 cut(s) 63
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 1 cut(s) 99
XceI RCATGY 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 4 cut(s) 5, 9, 315, 395
ZrmI AGTACT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.