Rh7AG150200

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0013768
Physical Location & Seq
Forward (+)
12168863 .. 12169765
903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG150200.1

Sequence Viewer

Length: 903 bp
ATGCCAACCATATTTCAAGTTTATGCGGTTGGCCAACAAAAGCAAATCAAGCCTCATCCATTATATTCCGTTAAAGAAAGGAATAGACTTGGTCTTAGATCTCGTGTGGTTGCCAAGTACAATGGACTGATCTTACTTCAAGTTCAAGTTCTCCTCCGGAAGAAGTACTACCTTGTTCTATGGAATCCGACGATTGCCAAGCATAGAAGGATCCCCTATGAGTTCGAGAAACATAAACGATTCCATAGGGTTTTCGGAGTAGGGTATGATTCTGATGCAGACGACTTGAAGGTCTGTACTCTTGAGCAACTATCCGATTTGAGTACCGAATTAAAAGTTTACAGTCTCGCATCTGGCACATGCGAAACCATAAAACTAGAACTTCCATATCATAATTTGCTACTTATTAGAACACCTGTGGTGTTGATGAACAATTCTCGTGTAGCTTGGGTGATGAAAAAGGTCGCATCTGATTATGAGGCAGGTCGTCACTCCATTGTAACTTTTGACCTAGTGAGGAAGGAGTTTCATGGCTTTCCTCTTCCCCGACCATTTAAAAATTTTGCGCGCGGAGAATTGGTGGTGTTGGGAGGCCTGTTATCCATTTGGTATGAAAACTGGGTTTGGATTATGAAGGAAGAATATTCTTGGACTAAGCTTCACTCTTTGCTCTTCAGTTCTGCCCAGCCCCTATTGTTTGATGGTGATCGGGGGAGGGTTCTCTTGAAAGTTGAGCTGAGTAATGGTGGTGCACGGCTTGAGTGGTTCACTTTAAAGACAGGAGAGATGACAAAGATTGATGGGTGCTGTACTCTCCCAGGTTCAGGTGTTCATGTTTGTGAGGGAAACATTCGTCTTCTCCTCGATGGTCATCCTGATGGGAAGCATAGTTACTGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

300

Amino Acids

34.66

Weight (kDa)

9.42

Isoelectric Point (pI)

26.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 18 - 222 3.1e-10 F-box associated beta propeller domain
FBA_3 PF08268 33 - 220 3.3e-07 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 290
Acc36I ACCTGC 1 cut(s) 473
AccII CGCG 2 cut(s) 568, 570
AccIII TCCGGA 1 cut(s) 156
AciI CCGC 2 cut(s) 26, 570
AclWI GGATC 2 cut(s) 205, 218
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 1 cut(s) 559
AcuI CTGAAG 1 cut(s) 658
AfaI GTAC 5 cut(s) 119, 167, 298, 325, 811
AfiI CCNNNNNNNGG 1 cut(s) 824
AgsI TTSAA 5 cut(s) 17, 140, 146, 289, 727
AjnI CCWGG 1 cut(s) 817
AluBI AGCT 3 cut(s) 446, 658, 736
AluI AGCT 3 cut(s) 446, 658, 736
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 1 cut(s) 350
Alw44I GTGCAC 1 cut(s) 750
AlwI GGATC 2 cut(s) 205, 218
Aor13HI TCCGGA 1 cut(s) 156
AoxI GGCC 2 cut(s) 31, 592
ApaLI GTGCAC 1 cut(s) 750
ApoI RAATTY 1 cut(s) 559
AspLEI GCGC 2 cut(s) 568, 570
AsuHPI GGTGA 2 cut(s) 463, 716
BaeGI GKGCMC 1 cut(s) 754
BalI TGGCCA 1 cut(s) 33
BamHI GGATCC 1 cut(s) 210
BarI GAAGNNNNNNTAC 3 cut(s) 152, 184, 875
BauI CACGAG 2 cut(s) 102, 438
BbsI GAAGAC 1 cut(s) 848
Bbv12I GWGCWC 1 cut(s) 754
BccI CCATC 4 cut(s) 695, 794, 860, 872
BceAI ACGGC 1 cut(s) 770
BciT130I CCWGG 1 cut(s) 819
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 2 cut(s) 377, 512
BfuAI ACCTGC 1 cut(s) 473
BglII AGATCT 1 cut(s) 98
BmcAI AGTACT 1 cut(s) 167
Bme1390I CCNGG 1 cut(s) 819
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 1 cut(s) 819
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 3 cut(s) 265, 359, 476
BmuI ACTGGG 1 cut(s) 628
BpiI GAAGAC 1 cut(s) 848
BpuEI CTTGAG 2 cut(s) 323, 779
BsaBI GATNNNNATC 2 cut(s) 705, 870
BsaJI CCNNGG 1 cut(s) 817
BsaWI WCCGGW 1 cut(s) 156
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 824
Bse1I ACTGG 2 cut(s) 623, 899
Bse8I GATNNNNATC 2 cut(s) 705, 870
BseAI TCCGGA 1 cut(s) 156
BseBI CCWGG 1 cut(s) 819
BseDI CCNNGG 1 cut(s) 817
BseGI GGATG 2 cut(s) 55, 871
BseJI GATNNNNATC 2 cut(s) 705, 870
BseLI CCNNNNNNNGG 1 cut(s) 824
BseMII CTCAG 1 cut(s) 728
BseNI ACTGG 2 cut(s) 623, 899
BsePI GCGCGC 1 cut(s) 566
BseRI GAGGAG 2 cut(s) 143, 851
BseSI GKGCMC 1 cut(s) 754
BseYI CCCAGC 1 cut(s) 684
Bsh1236I CGCG 2 cut(s) 568, 570
BshFI GGCC 2 cut(s) 33, 594
BsiHKAI GWGCWC 1 cut(s) 754
BsiSI CCGG 1 cut(s) 157
BslI CCNNNNNNNGG 1 cut(s) 824
BsmAI GTCTC 1 cut(s) 350
BsnI GGCC 2 cut(s) 33, 594
Bsp1286I GDGCHC 1 cut(s) 754
Bsp13I TCCGGA 1 cut(s) 156
Bsp143I GATC 4 cut(s) 98, 129, 210, 706
BspACI CCGC 2 cut(s) 26, 570
BspANI GGCC 2 cut(s) 33, 594
BspCNI CTCAG 1 cut(s) 729
BspEI TCCGGA 1 cut(s) 156
BspFNI CGCG 2 cut(s) 568, 570
BspLI GGNNCC 1 cut(s) 212
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 2 cut(s) 205, 218
BspQI GCTCTTC 1 cut(s) 677
BsrI ACTGG 2 cut(s) 623, 899
BssECI CCNNGG 1 cut(s) 817
BssHII GCGCGC 1 cut(s) 566
BssMI GATC 4 cut(s) 98, 129, 210, 706
BssSI CACGAG 2 cut(s) 102, 438
Bst2BI CACGAG 2 cut(s) 102, 438
Bst2UI CCWGG 1 cut(s) 819
Bst4CI ACNGT 1 cut(s) 344
Bst6I CTCTTC 2 cut(s) 546, 677
BstC8I GCNNGC 1 cut(s) 568
BstDEI CTNAG 3 cut(s) 95, 654, 737
BstF5I GGATG 2 cut(s) 55, 871
BstFNI CGCG 2 cut(s) 568, 570
BstHHI GCGC 2 cut(s) 568, 570
BstKTI GATC 4 cut(s) 101, 132, 213, 709
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 4 cut(s) 98, 129, 210, 706
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNI CCWGG 1 cut(s) 819
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 817
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 2 cut(s) 568, 570
BstV2I GAAGAC 1 cut(s) 848
BstX2I RGATCY 2 cut(s) 98, 210
BstYI RGATCY 2 cut(s) 98, 210
BsuRI GGCC 2 cut(s) 33, 594
BtsCI GGATG 2 cut(s) 55, 871
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 1 cut(s) 568
CfoI GCGC 2 cut(s) 568, 570
Csp6I GTAC 5 cut(s) 118, 166, 297, 324, 810
CviAII CATG 3 cut(s) 360, 530, 833
CviJI RGCY 9 cut(s) 33, 52, 446, 534, 594, 658, 688, 736, 757
CviKI_1 RGCY 9 cut(s) 33, 52, 446, 534, 594, 658, 688, 736, 757
CviQI GTAC 5 cut(s) 118, 166, 297, 324, 810
DdeI CTNAG 3 cut(s) 95, 654, 737
DpnI GATC 4 cut(s) 100, 131, 212, 708
DpnII GATC 4 cut(s) 98, 129, 210, 706
DraI TTTAAA 2 cut(s) 556, 774
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 2 cut(s) 546, 677
EarI CTCTTC 2 cut(s) 546, 677
Eco147I AGGCCT 1 cut(s) 594
Eco57I CTGAAG 1 cut(s) 658
EcoRII CCWGG 1 cut(s) 817
FaeI CATG 3 cut(s) 363, 533, 836
FatI CATG 3 cut(s) 359, 529, 832
FokI GGATG 2 cut(s) 42, 858
FspBI CTAG 2 cut(s) 377, 512
GlaI GCGC 2 cut(s) 567, 569
GsaI CCCAGC 1 cut(s) 688
HaeIII GGCC 2 cut(s) 33, 594
HapII CCGG 1 cut(s) 157
HhaI GCGC 2 cut(s) 568, 570
Hin1II CATG 3 cut(s) 363, 533, 836
Hin6I GCGC 2 cut(s) 566, 568
HinP1I GCGC 2 cut(s) 566, 568
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 3 cut(s) 184, 240, 269
HpaII CCGG 1 cut(s) 157
HphI GGTGA 2 cut(s) 463, 716
Hpy166II GTNNAC 3 cut(s) 340, 752, 768
Hpy188I TCNGA 5 cut(s) 189, 257, 274, 316, 472
Hpy188III TCNNGA 5 cut(s) 157, 226, 302, 724, 875
Hpy8I GTNNAC 3 cut(s) 340, 752, 768
Hpy99I CGWCG 1 cut(s) 193
HpyAV CCTTC 4 cut(s) 201, 283, 514, 628
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4V TGCA 2 cut(s) 278, 752
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 3 cut(s) 95, 654, 737
Hsp92II CATG 3 cut(s) 363, 533, 836
HspAI GCGC 2 cut(s) 566, 568
Kpn2I TCCGGA 1 cut(s) 156
Kzo9I GATC 4 cut(s) 98, 129, 210, 706
LguI GCTCTTC 1 cut(s) 677
LweI GCATC 3 cut(s) 265, 359, 476
MaeI CTAG 2 cut(s) 377, 512
MaeIII GTNAC 3 cut(s) 488, 499, 890
MalI GATC 4 cut(s) 100, 131, 212, 708
MboI GATC 4 cut(s) 98, 129, 210, 706
MboII GAAGA 5 cut(s) 172, 533, 650, 664, 848
MflI RGATCY 2 cut(s) 98, 210
MhlI GDGCHC 1 cut(s) 754
MlsI TGGCCA 1 cut(s) 33
MluCI AATT 5 cut(s) 329, 394, 433, 559, 575
MluNI TGGCCA 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 212
MnlI CCTC 9 cut(s) 63, 164, 472, 510, 549, 584, 708, 835, 872
Mox20I TGGCCA 1 cut(s) 33
MroI TCCGGA 1 cut(s) 156
MscI TGGCCA 1 cut(s) 33
MseI TTAA 4 cut(s) 72, 332, 555, 773
MslI CAYNNNNRTG 2 cut(s) 837, 876
Msp20I TGGCCA 1 cut(s) 33
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 1 cut(s) 819
MvaI CCWGG 1 cut(s) 819
MvnI CGCG 2 cut(s) 568, 570
MwoI GCNNNNNNNGC 1 cut(s) 49
NdeII GATC 4 cut(s) 98, 129, 210, 706
NlaIII CATG 3 cut(s) 363, 533, 836
NlaIV GGNNCC 1 cut(s) 212
NmuCI GTSAC 1 cut(s) 488
NspI RCATGY 1 cut(s) 363
PauI GCGCGC 1 cut(s) 566
PceI AGGCCT 1 cut(s) 594
PciSI GCTCTTC 1 cut(s) 677
PfeI GAWTC 3 cut(s) 184, 240, 269
PflFI GACNNNGTC 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 817
PspFI CCCAGC 1 cut(s) 684
PspGI CCWGG 1 cut(s) 817
PspN4I GGNNCC 1 cut(s) 212
PsuI RGATCY 2 cut(s) 98, 210
PsyI GACNNNGTC 1 cut(s) 90
PteI GCGCGC 1 cut(s) 566
RsaI GTAC 5 cut(s) 119, 167, 298, 325, 811
RsaNI GTAC 5 cut(s) 118, 166, 297, 324, 810
RseI CAYNNNNRTG 2 cut(s) 837, 876
SapI GCTCTTC 1 cut(s) 677
SaqAI TTAA 4 cut(s) 72, 332, 555, 773
Sau3AI GATC 4 cut(s) 98, 129, 210, 706
ScaI AGTACT 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 819
SduI GDGCHC 1 cut(s) 754
SfaNI GCATC 3 cut(s) 265, 359, 476
SmiMI CAYNNNNRTG 2 cut(s) 837, 876
SmlI CTYRAG 2 cut(s) 302, 758
SmoI CTYRAG 2 cut(s) 302, 758
Sse9I AATT 5 cut(s) 329, 394, 433, 559, 575
SseBI AGGCCT 1 cut(s) 594
SsiI CCGC 2 cut(s) 26, 570
SspI AATATT 1 cut(s) 644
SspMI CTAG 2 cut(s) 377, 512
StuI AGGCCT 1 cut(s) 594
StyD4I CCNGG 1 cut(s) 817
TaaI ACNGT 1 cut(s) 344
TaqI TCGA 2 cut(s) 225, 864
TasI AATT 5 cut(s) 329, 394, 433, 559, 575
TatI WGTACW 4 cut(s) 117, 165, 296, 809
TfiI GAWTC 3 cut(s) 184, 240, 269
Tru1I TTAA 4 cut(s) 72, 332, 555, 773
Tru9I TTAA 4 cut(s) 72, 332, 555, 773
TseFI GTSAC 1 cut(s) 488
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 6 cut(s) 443, 470, 518, 627, 647, 821
TspGWI ACGGA 1 cut(s) 58
Tth111I GACNNNGTC 1 cut(s) 90
VneI GTGCAC 1 cut(s) 750
XapI RAATTY 1 cut(s) 559
XceI RCATGY 1 cut(s) 363
XspI CTAG 2 cut(s) 377, 512
ZrmI AGTACT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.