Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0004411

Physical Location & Seq

Genomic Coordinates Forward (+)

33641809 .. 33645177

Gene Structure 3369 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG194900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2724 bp

ATGAGTATAATTGGGGGTAAGTGGGATGATGATGAGTGCTCTGTCATTGGAGACAAAGGGGATATCGGGTTCATCGATTTCAAAGATGACAAATCGGTTTGTAGTTACAACCCAGCTGAAGAGGGTCCGATTGTTATTTCAGTCCCATTCCCTCTTGTGGGAGGAAAGCAAGGTCAAAAGCCTCGATCAGTAGTTGTAGGAGAAACAGTTTCGGATAAAATAACAATTGAGAACACCACCCGTAAGCCAGTGGAGCTATGGGGTGTTAAAATTTATGCCTCAACTCCAGAGGACTCTTTCACACTTTCTCTGATGGAGCCTCCAAAGAAAGACTCTGGTGTAGAGGCCATTAGAGCCTTCCTTGAGTCTGCTTCCCTCGAGGACAGAGTGCTGCAACCAGGAGACACTTTGACTGTGTGGCTGTCTTGCAAGCCCAAGGAAATTGGTATGCACACCAGCGTTGTGCAGTTTGATTTCGAGGATAATCAGATTGAACGTGCTGTTTTTCTCTTGGCTGATGACAAAATCTCCCAATCTCTGGCCTCAACAAGACCATTCGAAAGACGCACTAAAAAGAAACCAATTGTTGTGGATTCCTATCATGTGGCTGTAAGCCCTAGTAGAAAAACAAAACGCCTACCCTACAAAAATAGGCTTCCGCGATATGACATTCCAAAGGAACTTAGGGATTACCTCTTGAGCGGGCACGATCCTGAATGTGTTACAGAAGGTCTTAAGAGGCGGAACTATGCTGATCACTTCAAGACTTTACTGATGATGGAGGAAATACAGTTAGAGGAAAGCATGAGAAGTTATGATATGGAATATGTTAGTTTGAGGAGGAGGGGATATCATTATTTGTCCCTTGAAGTCCCAGGGCTTGCTGAGAGAAGGCCTTCACTTGTCCAAGGGGATCATGTTTTCGCCAAGCTTTCTGAGTATGCAGATGACACAACACTTAATCCATATCAGGGTTTCATTCACCATGTAGAGGCTGATGAAGTTTACTTGAAGTTTGCGCAAGATTTTCACAGTGACCACAGAGACGGTAATCTTTATAACGTGCAATTCACATATAATCGAGTTTCCATGAGAAGGTTATATCAAGCGACTGATGCTGCTGCAGAGTTAGACAGAGGGTTCCTATTTCCATCTGAGTCCCCTCAAAGATGGATTAATGCCCCAAAACTGTACCCTATACCTTCTTGTAGACTTAATGAAGAGCAGATGCGTGCAGTTGAGATGATCCTTGGCTGCAAAGGAGGACCACCTTATGTGATTCATGGACCTCCTGGCACAGGCAAGACAATGACAATAGTGGAAGCAATCCTCCAACTCTACAAAAGTCAAGCAAAAGCTCGAGTTCTTGTTTGTGCACCTTCAAATAGTGCAGCAGACCACATCCTCGAGAAACTCCTCAATGAAAAGGCTGACGTAGTAGTTAAAGAACATGAAATATTCAGGCTTAATGCACCTACCCGTCCTTATGAAGATATCAAGCCTGATTATATTAAATTTTGCTCCTTCGAGGACAATATCTTCAACTGTCCTCCTCCCAAAGCCCTCAGGCGCTATAGGATCATCATATCAACCTATATGAGTGCCGCTCTTCTTTATGCAGAAGGTATCGAAAAAGGCCACTTCTCTCATATTTTCTTGGATGAGGCAGGACAAGCTTCAGAACCAGAAACCATGATCCCAGTAGCTAGTCTGCATGAGAGGAATACAGTTGTTGTTCTTGCTGGAGATCCTATGCAATTAGGGCCGATCATAAACTCCAAGAAAGCAGAAGATTACTATTTGGGGATATCATACTTGGAGAGGATGTTCGACTGTGAATTTTATAGAGATGAAGATTACAGTTACGTGACAAAGTTGGTTAGAAATTACAGATGTCACCCAGACATTTTGCATCTCCCCAATGAGTTATTCTATGGAGGAGAGTTGATTGCTTGTAAAGATGACTCAGTTTCCATCATTACAAGGCTGGACTTTCTTCCGAATAAGGACTTCCCCGTGGTTTTCTTTGGCATTCAAGGTTGTGATGAGAGGGAAGGAAACAATCCATCATGGTTTAACCGGACTGAAGCTAGTAAGGTAGTGGAGGTCACTAAGAGATTGACTGCCGGAAAGAACCTGAGTGAAGAAGATATTGGGGTTATAACTCCTTACCGACAGCAAGTGCTCAAAGTGAAGAAAGCTTTTGAAGATTTGGACATGCCTGACATCAAGGTTGGAAGTGTCGAGCAGTTTCAGGGACAGGAGAGGGAAGTTATCATAATATCAACTGTGCGATCAACAATCAAGCACAATGAATTTGACAGAACATACTGTTTGGGATTTCTGAGCAATCCAAAACGGTTTAATGTGGCTATTACTCGTGCCAAATCTTTGCTGATTATAATTGGGAACCCACATATTATCAGCAAGGATCCCAACTGGAATAAGCTTCTGTGGCGCTGTGCAGACAACAATTCCTATCAGGGTTGTAATCTCCCTGTCAGGCAGGAGGAATTTTCTTATGAGAACCCAACACAGGAAGATAACTGGGACAACAGTGAAGAAAACGCTCAGTTCTCTAGAACTGATTCATGGGTTCAAGGATCGTGGGACGCAGAAGCTCCGCAACCTATCCAGAACGGAGATGGAGCTGAACAGTGGCTGGAAACAGAAGTTCCTCAGCCTGTCGTTGATGAATCTGAATGGTCTGATGGCTGGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003724 GO:0003824 GO:0004386 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019222 GO:0031047 GO:0035194 GO:0035821 GO:0040029 GO:0043207 GO:0044003 GO:0044403 GO:0044419 GO:0045087 GO:0048519 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0098542 GO:0098586 GO:0140098

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_11 AAA_12

Protein Analysis

907

Amino Acids

103.52

Weight (kDa)

5.28

Isoelectric Point (pI)

47.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MOV-10_beta-barrel	PF21634	272 - 357	3.4e-31	Helicase MOV-10, beta-barrel domain
ResIII	PF04851	403 - 563	1.3e-08	Type III restriction enzyme, res subunit
AAA_11	PF13086	405 - 494	1.4e-20	AAA domain
AAA_30	PF13604	405 - 592	1.2e-13	AAA domain
AAA_19	PF13245	423 - 589	4.6e-09	AAA domain
AAA_11	PF13086	520 - 594	7.5e-12	AAA domain
AAA_12	PF13087	604 - 805	1.2e-49	AAA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004411)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05460 AT1G05460
fragaria_vesca	FvH4_4g15972 FvH4_4g15972 FvH4_4g15972 FvH4_4g15972 FvH4_4g15972 FvH4_4g15972 FvH4_4g15972
malus_domestica	MD13G1196500.v1.1
prunus_persica	Prupe.1G017300_v2.0.a1 Prupe.1G017300_v2.0.a1 Prupe.1G017300_v2.0.a1 Prupe.1G017300_v2.0.a1 Prupe.1G017300_v2.0.a1 Prupe.1G017300_v2.0.a1
pyrus_communis	pycom13g16860
rosa_chinensis	RchiOBHm_Chr4g0415581
rosa_laevigata	RLG00000008062
rosa_multiflora	Rmu_sc0004599.1_g000006
rosa_roxburghii	Rroxscaffold_5G00359390
rosa_rugosa	Rorug04G0134800 Rorug04G0134900 Rorug04G0135000 Rorug04G0135100 Rorug04G0135200 Rorug04G0135300
rosa_samantha	Rh4AG196200 Rh4BG194900 Rh4CG206900 Rh4DG193800
rosa_wichuraiana	Rw4G016680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1059, 2162, 2402
AbsI	CCTCGAGG	1 cut(s)	377
Acc16I	TGCGCA	1 cut(s)	1020
AccB7I	CCANNNNNTGG	1 cut(s)	538
AccBSI	CCGCTC	2 cut(s)	702, 1607
AccI	GTMKAC	1 cut(s)	1210
AccII	CGCG	1 cut(s)	661
AciI	CCGC	5 cut(s)	659, 702, 742, 1605, 2624
AclWI	GGATC	9 cut(s)	704, 921, 1240, 1586, 1690, 1742, 2426, 2439, 2611
AcsI	RAATTY	5 cut(s)	270, 1514, 1838, 2315, 2513
AcuI	CTGAAG	3 cut(s)	138, 1662, 2106
AfaI	GTAC	1 cut(s)	1193
AfiI	CCNNNNNNNGG	8 cut(s)	157, 158, 538, 971, 991, 1095, 1298, 2536
AflII	CTTAAG	1 cut(s)	734
AjnI	CCWGG	3 cut(s)	397, 874, 1291
AjuI	GAANNNNNNNTTGG	4 cut(s)	1913, 1945, 2136, 2168
AloI	GAACNNNNNNTCC	4 cut(s)	53, 85, 1811, 1843
Alw21I	GWGCWC	3 cut(s)	41, 1378, 2187
Alw26I	GTCTC	3 cut(s)	45, 396, 1038
Alw44I	GTGCAC	1 cut(s)	1374
AlwI	GGATC	9 cut(s)	704, 921, 1240, 1586, 1690, 1742, 2426, 2439, 2611
AlwNI	CAGNNNCTG	1 cut(s)	2662
Ama87I	CYCGRG	3 cut(s)	377, 1359, 1406
AoxI	GGCC	5 cut(s)	345, 540, 893, 1636, 1763
ApaLI	GTGCAC	1 cut(s)	1374
ApeKI	GCWGC	5 cut(s)	391, 1118, 1121, 1254, 1391
ApoI	RAATTY	5 cut(s)	270, 1514, 1838, 2315, 2513
AseI	ATTAAT	1 cut(s)	1176
Asp700I	GAANNNNTTC	2 cut(s)	895, 2587
AspLEI	GCGC	3 cut(s)	1021, 1572, 2460
AspS9I	GGNCC	4 cut(s)	125, 1265, 1286, 1763
AsuHPI	GGTGA	2 cut(s)	974, 1889
AsuII	TTCGAA	1 cut(s)	558
AvaI	CYCGRG	3 cut(s)	377, 1359, 1406
AvaII	GGWCC	3 cut(s)	125, 1265, 1286
AxyI	CCTNAGG	1 cut(s)	1565
BaeGI	GKGCMC	2 cut(s)	708, 1378
BamHI	GGATCC	1 cut(s)	2431
BauI	CACGAG	1 cut(s)	2379
Bbv12I	GWGCWC	3 cut(s)	41, 1378, 2187
BbvCI	CCTCAGC	1 cut(s)	2679
BbvI	GCAGC	5 cut(s)	378, 1105, 1108, 1241, 1403
BccI	CCATC	8 cut(s)	307, 772, 1159, 1164, 1982, 2074, 2639, 2705
BciT130I	CCWGG	3 cut(s)	399, 876, 1293
BclI	TGATCA	1 cut(s)	754
BcoDI	GTCTC	3 cut(s)	45, 396, 1038
BfaI	CTAG	4 cut(s)	618, 1707, 2091, 2580
BfmI	CTRYAG	2 cut(s)	1122, 1573
BfoI	RGCGCY	2 cut(s)	1573, 2461
BfrI	CTTAAG	1 cut(s)	734
BisI	GCNGC	6 cut(s)	392, 1119, 1122, 1255, 1392, 1605
BlsI	GCNGC	6 cut(s)	393, 1120, 1123, 1256, 1393, 1606
Bme1390I	CCNGG	3 cut(s)	399, 876, 1293
Bme18I	GGWCC	3 cut(s)	125, 1265, 1286
BmeT110I	CYCGRG	3 cut(s)	377, 1359, 1406
BmgT120I	GGNCC	4 cut(s)	125, 1265, 1286, 1763
BmiI	GGNNCC	5 cut(s)	126, 318, 1142, 2411, 2433
BmrFI	CCNGG	3 cut(s)	399, 876, 1293
BmrI	ACTGGG	2 cut(s)	1694, 2557
BmsI	GCATC	3 cut(s)	1105, 1218, 1921
BmuI	ACTGGG	2 cut(s)	1694, 2557
BplI	GAGNNNNNCTC	2 cut(s)	1591, 1623
BpmI	CTGGAG	2 cut(s)	270, 1764
Bpu10I	CCTNAGC	1 cut(s)	2679
Bpu14I	TTCGAA	1 cut(s)	558
BpuEI	CTTGAG	2 cut(s)	383, 718
Bsa29I	ATCGAT	1 cut(s)	75
BsaAI	YACGTR	1 cut(s)	1867
BsaBI	GATNNNNATC	1 cut(s)	597
BsaJI	CCNNGG	6 cut(s)	435, 874, 875, 907, 1249, 2016
BsaWI	WCCGGW	1 cut(s)	2079
BsaXI	ACNNNNNCTCC	6 cut(s)	192, 222, 512, 542, 1811, 1841
Bsc4I	CCNNNNNNNGG	8 cut(s)	157, 158, 538, 971, 991, 1095, 1298, 2536
Bse1I	ACTGG	4 cut(s)	248, 1700, 2444, 2552
Bse21I	CCTNAGG	1 cut(s)	1565
Bse8I	GATNNNNATC	1 cut(s)	597
BseBI	CCWGG	3 cut(s)	399, 876, 1293
BseCI	ATCGAT	1 cut(s)	75
BseDI	CCNNGG	6 cut(s)	435, 874, 875, 907, 1249, 2016
BseGI	GGATG	4 cut(s)	31, 1401, 1666, 1830
BseJI	GATNNNNATC	1 cut(s)	597
BseLI	CCNNNNNNNGG	8 cut(s)	157, 158, 538, 971, 991, 1095, 1298, 2536
BseMII	CTCAG	9 cut(s)	876, 927, 1146, 1579, 1980, 2129, 2336, 2585, 2693
BseNI	ACTGG	4 cut(s)	248, 1700, 2444, 2552
BseRI	GAGGAG	5 cut(s)	853, 856, 1406, 1542, 1953
BseSI	GKGCMC	2 cut(s)	708, 1378
BseXI	GCAGC	5 cut(s)	378, 1105, 1108, 1241, 1403
BseYI	CCCAGC	1 cut(s)	112
BsgI	GTGCAG	4 cut(s)	485, 1254, 1410, 2484
Bsh1236I	CGCG	1 cut(s)	661
BshFI	GGCC	5 cut(s)	347, 542, 895, 1638, 1765
BshVI	ATCGAT	1 cut(s)	75
BsiHKAI	GWGCWC	3 cut(s)	41, 1378, 2187
BsiHKCI	CYCGRG	3 cut(s)	377, 1359, 1406
BsiSI	CCGG	2 cut(s)	2080, 2127
BslFI	GGGAC	7 cut(s)	128, 847, 857, 1144, 2271, 2564, 2624
BslI	CCNNNNNNNGG	8 cut(s)	157, 158, 538, 971, 991, 1095, 1298, 2536
BsmAI	GTCTC	3 cut(s)	45, 396, 1038
BsmBI	CGTCTC	1 cut(s)	1038
BsmFI	GGGAC	7 cut(s)	128, 847, 857, 1144, 2271, 2564, 2624
BsmI	GAATGC	1 cut(s)	2031
BsnI	GGCC	5 cut(s)	347, 542, 895, 1638, 1765
BsoBI	CYCGRG	3 cut(s)	377, 1359, 1406
Bsp119I	TTCGAA	1 cut(s)	558
Bsp1286I	GDGCHC	4 cut(s)	41, 708, 1378, 2187
BspACI	CCGC	5 cut(s)	659, 702, 742, 1605, 2624
BspANI	GGCC	5 cut(s)	347, 542, 895, 1638, 1765
BspCNI	CTCAG	9 cut(s)	877, 928, 1147, 1578, 1979, 2130, 2337, 2584, 2692
BspDI	ATCGAT	1 cut(s)	75
BspFNI	CGCG	1 cut(s)	661
BspLI	GGNNCC	5 cut(s)	126, 318, 1142, 2411, 2433
BspMAI	CTGCAG	1 cut(s)	1126
BspPI	GGATC	9 cut(s)	704, 921, 1240, 1586, 1690, 1742, 2426, 2439, 2611
BspQI	GCTCTTC	2 cut(s)	1215, 1614
BspT104I	TTCGAA	1 cut(s)	558
BspTI	CTTAAG	1 cut(s)	734
BsrBI	CCGCTC	2 cut(s)	702, 1607
BsrI	ACTGG	4 cut(s)	248, 1700, 2444, 2552
BssECI	CCNNGG	6 cut(s)	435, 874, 875, 907, 1249, 2016
BssSI	CACGAG	1 cut(s)	2379
BssT1I	CCWWGG	3 cut(s)	435, 907, 1249
Bst2BI	CACGAG	1 cut(s)	2379
Bst2UI	CCWGG	3 cut(s)	399, 876, 1293
Bst6I	CTCTTC	3 cut(s)	114, 1215, 1614
BstAFI	CTTAAG	1 cut(s)	734
BstBAI	YACGTR	1 cut(s)	1867
BstBI	TTCGAA	1 cut(s)	558
BstC8I	GCNNGC	4 cut(s)	431, 704, 882, 1233
BstDSI	CCRYGG	1 cut(s)	2016
BstENI	CCTNNNNNAGG	1 cut(s)	1296
BstF5I	GGATG	4 cut(s)	31, 1401, 1666, 1830
BstFNI	CGCG	1 cut(s)	661
BstH2I	RGCGCY	2 cut(s)	1573, 2461
BstHHI	GCGC	3 cut(s)	1021, 1572, 2460
BstMAI	GTCTC	3 cut(s)	45, 396, 1038
BstMWI	GCNNNNNNNGC	6 cut(s)	253, 353, 1115, 1673, 1762, 2455
BstNI	CCWGG	3 cut(s)	399, 876, 1293
BstNSI	RCATGY	1 cut(s)	2221
BstSCI	CCNGG	3 cut(s)	397, 874, 1291
BstSFI	CTRYAG	2 cut(s)	1122, 1573
BstSLI	GKGCMC	2 cut(s)	708, 1378
BstUI	CGCG	1 cut(s)	661
BstV1I	GCAGC	5 cut(s)	378, 1105, 1108, 1241, 1403
BstX2I	RGATCY	2 cut(s)	1747, 2431
BstYI	RGATCY	2 cut(s)	1747, 2431
Bsu15I	ATCGAT	1 cut(s)	75
Bsu36I	CCTNAGG	1 cut(s)	1565
BsuRI	GGCC	5 cut(s)	347, 542, 895, 1638, 1765
BsuTUI	ATCGAT	1 cut(s)	75
BtgI	CCRYGG	1 cut(s)	2016
BtsCI	GGATG	4 cut(s)	31, 1401, 1666, 1830
BtsIMutI	CAGTG	4 cut(s)	255, 1039, 2563, 2663
Cac8I	GCNNGC	4 cut(s)	431, 704, 882, 1233
CaiI	CAGNNNCTG	1 cut(s)	2662
CfoI	GCGC	3 cut(s)	1021, 1572, 2460
Cfr13I	GGNCC	4 cut(s)	125, 1265, 1286, 1763
ClaI	ATCGAT	1 cut(s)	75
CseI	GACGC	2 cut(s)	573, 2621
Csp6I	GTAC	1 cut(s)	1192
CspCI	CAANNNNNGTGG	4 cut(s)	570, 605, 2588, 2623
CviQI	GTAC	1 cut(s)	1192
Eam1104I	CTCTTC	3 cut(s)	114, 1215, 1614
EarI	CTCTTC	3 cut(s)	114, 1215, 1614
EciI	GGCGGA	1 cut(s)	757
Eco130I	CCWWGG	3 cut(s)	435, 907, 1249
Eco147I	AGGCCT	1 cut(s)	895
Eco32I	GATATC	4 cut(s)	64, 851, 1495, 1809
Eco47I	GGWCC	3 cut(s)	125, 1265, 1286
Eco57I	CTGAAG	3 cut(s)	138, 1662, 2106
Eco81I	CCTNAGG	1 cut(s)	1565
Eco88I	CYCGRG	3 cut(s)	377, 1359, 1406
EcoNI	CCTNNNNNAGG	1 cut(s)	1296
EcoRII	CCWGG	3 cut(s)	397, 874, 1291
EcoRV	GATATC	4 cut(s)	64, 851, 1495, 1809
EcoT14I	CCWWGG	3 cut(s)	435, 907, 1249
ErhI	CCWWGG	3 cut(s)	435, 907, 1249
Esp3I	CGTCTC	1 cut(s)	1038
FaqI	GGGAC	7 cut(s)	128, 847, 857, 1144, 2271, 2564, 2624
FauI	CCCGC	1 cut(s)	695
FbaI	TGATCA	1 cut(s)	754
FblI	GTMKAC	1 cut(s)	1210
Fnu4HI	GCNGC	6 cut(s)	392, 1119, 1122, 1255, 1392, 1605
FokI	GGATG	4 cut(s)	38, 1388, 1673, 1837
Fsp4HI	GCNGC	6 cut(s)	392, 1119, 1122, 1255, 1392, 1605
FspBI	CTAG	4 cut(s)	618, 1707, 2091, 2580
FspI	TGCGCA	1 cut(s)	1020
GlaI	GCGC	3 cut(s)	1020, 1571, 2459
GluI	GCNGC	6 cut(s)	392, 1119, 1122, 1255, 1392, 1605
GsaI	CCCAGC	1 cut(s)	116
GsuI	CTGGAG	2 cut(s)	270, 1764
HaeII	RGCGCY	2 cut(s)	1573, 2461
HaeIII	GGCC	5 cut(s)	347, 542, 895, 1638, 1765
HapII	CCGG	2 cut(s)	2080, 2127
HgaI	GACGC	2 cut(s)	573, 2621
HhaI	GCGC	3 cut(s)	1021, 1572, 2460
Hin6I	GCGC	3 cut(s)	1019, 1570, 2458
HinP1I	GCGC	3 cut(s)	1019, 1570, 2458
HindIII	AAGCTT	4 cut(s)	929, 1674, 2199, 2447
HinfI	GANTC	9 cut(s)	293, 332, 365, 593, 1157, 1279, 1964, 2588, 2696
HpaII	CCGG	2 cut(s)	2080, 2127
HphI	GGTGA	2 cut(s)	974, 1889
Hpy166II	GTNNAC	3 cut(s)	1006, 1211, 1376
Hpy188III	TCNNGA	7 cut(s)	287, 697, 713, 763, 1408, 2580, 2635
Hpy8I	GTNNAC	3 cut(s)	1006, 1211, 1376
HpyCH4IV	ACGT	4 cut(s)	496, 1062, 1434, 1866
HpyF10VI	GCNNNNNNNGC	6 cut(s)	253, 353, 1115, 1673, 1762, 2455
HpySE526I	ACGT	4 cut(s)	496, 1062, 1434, 1866
HspAI	GCGC	3 cut(s)	1019, 1570, 2458
Ksp22I	TGATCA	1 cut(s)	754
LguI	GCTCTTC	2 cut(s)	1215, 1614
LmnI	GCTCC	5 cut(s)	253, 316, 1526, 2626, 2648
Lsp1109I	GCAGC	5 cut(s)	378, 1105, 1108, 1241, 1403
LweI	GCATC	3 cut(s)	1105, 1218, 1921
MaeI	CTAG	4 cut(s)	618, 1707, 2091, 2580
MaeII	ACGT	4 cut(s)	496, 1062, 1434, 1866
MaeIII	GTNAC	7 cut(s)	104, 721, 1034, 1862, 1867, 1895, 2107
MbiI	CCGCTC	2 cut(s)	702, 1607
MfeI	CAATTG	2 cut(s)	225, 582
MflI	RGATCY	2 cut(s)	1747, 2431
MhlI	GDGCHC	4 cut(s)	41, 708, 1378, 2187
MlyI	GAGTC	5 cut(s)	287, 326, 374, 1166, 1958
MmeI	TCCRAC	2 cut(s)	1357, 2215
MroXI	GAANNNNTTC	2 cut(s)	895, 2587
MspA1I	CMGCKG	1 cut(s)	116
MspCI	CTTAAG	1 cut(s)	734
MspI	CCGG	2 cut(s)	2080, 2127
MspR9I	CCNGG	3 cut(s)	399, 876, 1293
MunI	CAATTG	2 cut(s)	225, 582
Mva1269I	GAATGC	1 cut(s)	2031
MvaI	CCWGG	3 cut(s)	399, 876, 1293
MvnI	CGCG	1 cut(s)	661
MwoI	GCNNNNNNNGC	6 cut(s)	253, 353, 1115, 1673, 1762, 2455
NlaIV	GGNNCC	5 cut(s)	126, 318, 1142, 2411, 2433
NmuCI	GTSAC	4 cut(s)	1034, 1867, 1895, 2107
NsbI	TGCGCA	1 cut(s)	1020
NspI	RCATGY	1 cut(s)	2221
NspV	TTCGAA	1 cut(s)	558
PaeR7I	CTCGAG	3 cut(s)	377, 1359, 1406
PasI	CCCWGGG	1 cut(s)	875
PceI	AGGCCT	1 cut(s)	895
PciSI	GCTCTTC	2 cut(s)	1215, 1614
PcsI	WCGNNNNNNNCGW	1 cut(s)	72
PctI	GAATGC	1 cut(s)	2031
PdmI	GAANNNNTTC	2 cut(s)	895, 2587
PfeI	GAWTC	4 cut(s)	593, 1279, 2588, 2696
PflMI	CCANNNNNTGG	1 cut(s)	538
PkrI	GCNGC	6 cut(s)	393, 1120, 1123, 1256, 1393, 1606
PleI	GAGTC	5 cut(s)	287, 326, 373, 1165, 1958
PpsI	GAGTC	5 cut(s)	287, 326, 373, 1165, 1958
Ppu21I	YACGTR	1 cut(s)	1867
PshBI	ATTAAT	1 cut(s)	1176
PsiI	TTATAA	3 cut(s)	1059, 2162, 2402
Psp6I	CCWGG	3 cut(s)	397, 874, 1291
PspFI	CCCAGC	1 cut(s)	112
PspGI	CCWGG	3 cut(s)	397, 874, 1291
PspN4I	GGNNCC	5 cut(s)	126, 318, 1142, 2411, 2433
PspPI	GGNCC	4 cut(s)	125, 1265, 1286, 1763
PspXI	VCTCGAGB	2 cut(s)	377, 1359
PstI	CTGCAG	1 cut(s)	1126
PstNI	CAGNNNCTG	1 cut(s)	2662
PsuI	RGATCY	2 cut(s)	1747, 2431
PvuII	CAGCTG	1 cut(s)	116
RsaI	GTAC	1 cut(s)	1193
RsaNI	GTAC	1 cut(s)	1192
SapI	GCTCTTC	2 cut(s)	1215, 1614
SatI	GCNGC	6 cut(s)	392, 1119, 1122, 1255, 1392, 1605
Sau96I	GGNCC	4 cut(s)	125, 1265, 1286, 1763
SchI	GAGTC	5 cut(s)	287, 326, 374, 1166, 1958
ScrFI	CCNGG	3 cut(s)	399, 876, 1293
SduI	GDGCHC	4 cut(s)	41, 708, 1378, 2187
SfaNI	GCATC	3 cut(s)	1105, 1218, 1921
SfcI	CTRYAG	2 cut(s)	1122, 1573
Sfr274I	CTCGAG	3 cut(s)	377, 1359, 1406
SfuI	TTCGAA	1 cut(s)	558
SinI	GGWCC	3 cut(s)	125, 1265, 1286
SlaI	CTCGAG	3 cut(s)	377, 1359, 1406
SmlI	CTYRAG	6 cut(s)	362, 377, 697, 734, 1359, 1406
SmoI	CTYRAG	6 cut(s)	362, 377, 697, 734, 1359, 1406
SseBI	AGGCCT	1 cut(s)	895
SsiI	CCGC	5 cut(s)	659, 702, 742, 1605, 2624
SspI	AATATT	1 cut(s)	1458
SspMI	CTAG	4 cut(s)	618, 1707, 2091, 2580
StuI	AGGCCT	1 cut(s)	895
StyD4I	CCNGG	3 cut(s)	397, 874, 1291
StyI	CCWWGG	3 cut(s)	435, 907, 1249
TaiI	ACGT	4 cut(s)	499, 1065, 1437, 1869
TauI	GCSGC	1 cut(s)	1607
TfiI	GAWTC	4 cut(s)	593, 1279, 2588, 2696
TscAI	CASTG	4 cut(s)	255, 1039, 2563, 2663
TseFI	GTSAC	4 cut(s)	1034, 1867, 1895, 2107
TseI	GCWGC	5 cut(s)	391, 1118, 1121, 1254, 1391
Tsp45I	GTSAC	4 cut(s)	1034, 1867, 1895, 2107
TspGWI	ACGGA	1 cut(s)	2655
TspRI	CASTG	4 cut(s)	255, 1039, 2563, 2663
Van91I	CCANNNNNTGG	1 cut(s)	538
Vha464I	CTTAAG	1 cut(s)	734
VneI	GTGCAC	1 cut(s)	1374
VpaK11BI	GGWCC	3 cut(s)	125, 1265, 1286
VspI	ATTAAT	1 cut(s)	1176
XagI	CCTNNNNNAGG	1 cut(s)	1296
XapI	RAATTY	5 cut(s)	270, 1514, 1838, 2315, 2513
XbaI	TCTAGA	1 cut(s)	2579
XceI	RCATGY	1 cut(s)	2221
XcmI	CCANNNNNNNNNTGG	2 cut(s)	255, 2642
XhoI	CTCGAG	3 cut(s)	377, 1359, 1406
XmiI	GTMKAC	1 cut(s)	1210
XmnI	GAANNNNTTC	2 cut(s)	895, 2587
XspI	CTAG	4 cut(s)	618, 1707, 2091, 2580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.