Rh4BG209000

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011627
Physical Location & Seq
Reverse (-)
36048296 .. 36067220
18925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG209000.1

Sequence Viewer

Length: 981 bp
ATGAATGTAGCTATTCGTCTCCCTGCTTTCCATGTTTCTTTTATGCGGCAGGATTATTGGTTTAGTAGGATTATACTATCTGCTGATCCCATTTTGAATCCGGACGACTTGATGGTTCTAATTTTAAATAAAAGGGGTACTGTACCTTTGGCTTTCACTAAAGCAGGCCAAAACTTCTGGAGTTGTTACACTGAGAGAGATGGTGTCTGCTTTGAGGATGCTATATTTCATCAAGGCCAAGCCTATGCAATTGGAGTGGGGGGATCGGTTTTTTCACTCCAGCTGAATGATCACAGCAAGCTTTTAGACAAATTAGAACTTGCACCTCCATTGGGCTTAGATTCTGATGCTGATAGAGACCTTACTCTGGAGTCAAGCAATGGGGAACTATTGCATTCATGCATCAAAGTTGCAATGGACTTTGTCTCACCTGAAAATATCGGCGAGTGCTTTCACTTGACAGAAGCATTCCTCACACTTCCTGAAAATCATAGGGCTAAAGCAGTCAAATTGGAGATTGTACATATTAAAGGGGGAGAGATTTGGACTGGAAACAGAGAAAAGATAATATCCGGGAAAGAAGAAGAGAGATTGAAATTGAGCAGCCCCTACACTGGAACTCTATTCATCAATCCAGCCACGCTCTTTCGCCTACCCTGCTTTCGCTCACTCTTAATATACGAAAATTTGAATAGCAATTGGAGTGAATTATCATTCATCAAAGCCACCATCTTTGTTGCATACCCAAGCTCAGAAGCACAGCCATTCATCCAGAGTGAACAATTATCAAGAAGATTTGTTTCTTCAATTCAGTTCTCTAATTTAGACGAATCAGAAACGATTCGAGCTCTGTTTTCGGAATCCAAAAGAAAGAAGGAAAATCCAATTCCAAACGACTCCGATCGTCTTCTTGAGCATACAAGTGATGGCATCTACCAGCACAACGAAACGGGTCAAAAGATTGTTGGAAGGAGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

326

Amino Acids

36.89

Weight (kDa)

5.65

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 4 - 161 1.6e-13 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 1 cut(s) 46
AclWI GGATC 2 cut(s) 80, 271
AcsI RAATTY 1 cut(s) 685
AfaI GTAC 3 cut(s) 139, 144, 522
AfiI CCNNNNNNNGG 3 cut(s) 332, 367, 614
AgsI TTSAA 4 cut(s) 97, 595, 691, 807
AluBI AGCT 5 cut(s) 11, 283, 301, 750, 848
AluI AGCT 5 cut(s) 11, 283, 301, 750, 848
Alw21I GWGCWC 1 cut(s) 850
Alw26I GTCTC 3 cut(s) 23, 351, 430
AlwI GGATC 2 cut(s) 80, 271
Aor13HI TCCGGA 1 cut(s) 100
AoxI GGCC 2 cut(s) 166, 235
ApeKI GCWGC 1 cut(s) 603
ApoI RAATTY 1 cut(s) 685
Asp700I GAANNNNTTC 1 cut(s) 840
AsuC2I CCSGG 1 cut(s) 574
AsuHPI GGTGA 1 cut(s) 420
BanII GRGCYC 1 cut(s) 850
BbsI GAAGAC 1 cut(s) 899
Bbv12I GWGCWC 1 cut(s) 850
BbvI GCAGC 1 cut(s) 615
BccI CCATC 4 cut(s) 106, 194, 737, 920
BclI TGATCA 1 cut(s) 289
BcnI CCSGG 1 cut(s) 574
BcoDI GTCTC 3 cut(s) 23, 351, 430
BisI GCNGC 2 cut(s) 47, 604
BlsI GCNGC 2 cut(s) 48, 605
Bme1390I CCNGG 1 cut(s) 574
BmrFI CCNGG 1 cut(s) 574
BmsI GCATC 4 cut(s) 208, 337, 411, 939
BpiI GAAGAC 1 cut(s) 899
BpmI CTGGAG 3 cut(s) 199, 263, 389
BpuEI CTTGAG 1 cut(s) 932
BpuMI CCSGG 1 cut(s) 574
BsaI GGTCTC 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 100
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 367, 614
Bse1I ACTGG 2 cut(s) 553, 619
Bse3DI GCAATG 2 cut(s) 385, 420
BseAI TCCGGA 1 cut(s) 100
BseGI GGATG 3 cut(s) 223, 768, 981
BseLI CCNNNNNNNGG 3 cut(s) 332, 367, 614
BseMI GCAATG 2 cut(s) 385, 420
BseMII CTCAG 2 cut(s) 183, 765
BseNI ACTGG 2 cut(s) 553, 619
BseXI GCAGC 1 cut(s) 615
Bsh1285I CGRYCG 1 cut(s) 904
BshFI GGCC 2 cut(s) 168, 237
BsiEI CGRYCG 1 cut(s) 904
BsiHKAI GWGCWC 1 cut(s) 850
BsiSI CCGG 2 cut(s) 101, 573
BslI CCNNNNNNNGG 3 cut(s) 332, 367, 614
BsmAI GTCTC 3 cut(s) 23, 351, 430
BsmBI CGTCTC 1 cut(s) 23
BsmI GAATGC 2 cut(s) 394, 467
BsnI GGCC 2 cut(s) 168, 237
Bso31I GGTCTC 1 cut(s) 351
Bsp1286I GDGCHC 1 cut(s) 850
Bsp13I TCCGGA 1 cut(s) 100
Bsp1407I TGTACA 1 cut(s) 520
Bsp143I GATC 4 cut(s) 85, 263, 289, 901
BspACI CCGC 1 cut(s) 46
BspANI GGCC 2 cut(s) 168, 237
BspCNI CTCAG 2 cut(s) 184, 764
BspEI TCCGGA 1 cut(s) 100
BspPI GGATC 2 cut(s) 80, 271
BspTNI GGTCTC 1 cut(s) 351
BsrDI GCAATG 2 cut(s) 385, 420
BsrGI TGTACA 1 cut(s) 520
BsrI ACTGG 2 cut(s) 553, 619
BssMI GATC 4 cut(s) 85, 263, 289, 901
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 579
BstAUI TGTACA 1 cut(s) 520
BstC8I GCNNGC 2 cut(s) 166, 299
BstDEI CTNAG 3 cut(s) 192, 337, 751
BstF5I GGATG 3 cut(s) 223, 768, 981
BstKTI GATC 4 cut(s) 88, 266, 292, 904
BstMAI GTCTC 3 cut(s) 23, 351, 430
BstMBI GATC 4 cut(s) 85, 263, 289, 901
BstMCI CGRYCG 1 cut(s) 904
BstMWI GCNNNNNNNGC 1 cut(s) 657
BstSCI CCNGG 1 cut(s) 572
BstV1I GCAGC 1 cut(s) 615
BstV2I GAAGAC 1 cut(s) 899
BsuRI GGCC 2 cut(s) 168, 237
BtsCI GGATG 3 cut(s) 223, 768, 981
BtsIMutI CAGTG 2 cut(s) 189, 612
Cac8I GCNNGC 2 cut(s) 166, 299
Csp6I GTAC 3 cut(s) 138, 143, 521
CspCI CAANNNNNGTGG 4 cut(s) 237, 272, 715, 750
CviAII CATG 2 cut(s) 32, 399
CviQI GTAC 3 cut(s) 138, 143, 521
DdeI CTNAG 3 cut(s) 192, 337, 751
DpnI GATC 4 cut(s) 87, 265, 291, 903
DpnII GATC 4 cut(s) 85, 263, 289, 901
DraI TTTAAA 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
Ecl136II GAGCTC 1 cut(s) 848
Eco24I GRGCYC 1 cut(s) 850
Eco31I GGTCTC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 848
EcoICRI GAGCTC 1 cut(s) 848
EcoT22I ATGCAT 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 850
Esp3I CGTCTC 1 cut(s) 23
FaeI CATG 2 cut(s) 35, 402
FatI CATG 2 cut(s) 31, 398
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 2 cut(s) 47, 604
FokI GGATG 2 cut(s) 230, 755
FriOI GRGCYC 1 cut(s) 850
Fsp4HI GCNGC 2 cut(s) 47, 604
GluI GCNGC 2 cut(s) 47, 604
GsuI CTGGAG 3 cut(s) 199, 263, 389
HaeIII GGCC 2 cut(s) 168, 237
HapII CCGG 2 cut(s) 101, 573
Hin1II CATG 2 cut(s) 35, 402
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 7 cut(s) 97, 341, 371, 830, 841, 860, 896
HpaII CCGG 2 cut(s) 101, 573
HphI GGTGA 1 cut(s) 420
Hpy166II GTNNAC 1 cut(s) 779
Hpy188I TCNGA 5 cut(s) 346, 754, 835, 859, 901
Hpy188III TCNNGA 7 cut(s) 101, 178, 368, 482, 772, 789, 911
Hpy8I GTNNAC 1 cut(s) 779
HpyAV CCTTC 2 cut(s) 868, 963
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4V TGCA 6 cut(s) 248, 323, 394, 402, 413, 740
HpyF10VI GCNNNNNNNGC 1 cut(s) 657
HpyF3I CTNAG 3 cut(s) 192, 337, 751
Hsp92II CATG 2 cut(s) 35, 402
Kpn2I TCCGGA 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 4 cut(s) 85, 263, 289, 901
Lsp1109I GCAGC 1 cut(s) 615
LweI GCATC 4 cut(s) 208, 337, 411, 939
MaeIII GTNAC 1 cut(s) 185
MalI GATC 4 cut(s) 87, 265, 291, 903
MboI GATC 4 cut(s) 85, 263, 289, 901
MboII GAAGA 5 cut(s) 593, 596, 795, 804, 899
MfeI CAATTG 2 cut(s) 249, 697
MhlI GDGCHC 1 cut(s) 850
MlyI GAGTC 2 cut(s) 380, 890
MmeI TCCRAC 1 cut(s) 946
MnlI CCTC 4 cut(s) 208, 336, 482, 966
Mph1103I ATGCAT 1 cut(s) 404
MroI TCCGGA 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 840
MseI TTAA 3 cut(s) 125, 528, 674
MslI CAYNNNNRTG 1 cut(s) 921
MspA1I CMGCKG 1 cut(s) 283
MspI CCGG 2 cut(s) 101, 573
MspR9I CCNGG 1 cut(s) 574
MunI CAATTG 2 cut(s) 249, 697
Mva1269I GAATGC 2 cut(s) 394, 467
MwoI GCNNNNNNNGC 1 cut(s) 657
NciI CCSGG 1 cut(s) 574
NdeII GATC 4 cut(s) 85, 263, 289, 901
NlaIII CATG 2 cut(s) 35, 402
NsiI ATGCAT 1 cut(s) 404
PctI GAATGC 2 cut(s) 394, 467
PdmI GAANNNNTTC 1 cut(s) 840
PfeI GAWTC 5 cut(s) 97, 341, 830, 841, 860
PflFI GACNNNGTC 1 cut(s) 422
PfoI TCCNGGA 1 cut(s) 572
PkrI GCNGC 2 cut(s) 48, 605
Ple19I CGATCG 1 cut(s) 904
PleI GAGTC 2 cut(s) 379, 890
PpsI GAGTC 2 cut(s) 379, 890
Psp124BI GAGCTC 1 cut(s) 850
PsyI GACNNNGTC 1 cut(s) 422
PvuI CGATCG 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 283
RsaI GTAC 3 cut(s) 139, 144, 522
RsaNI GTAC 3 cut(s) 138, 143, 521
RseI CAYNNNNRTG 1 cut(s) 921
SacI GAGCTC 1 cut(s) 850
SaqAI TTAA 3 cut(s) 125, 528, 674
SatI GCNGC 2 cut(s) 47, 604
Sau3AI GATC 4 cut(s) 85, 263, 289, 901
SchI GAGTC 2 cut(s) 380, 890
ScrFI CCNGG 1 cut(s) 574
SduI GDGCHC 1 cut(s) 850
SetI ASST 9 cut(s) 13, 148, 285, 303, 328, 363, 433, 752, 850
SfaNI GCATC 4 cut(s) 208, 337, 411, 939
SmiMI CAYNNNNRTG 1 cut(s) 921
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
SsiI CCGC 1 cut(s) 46
SstI GAGCTC 1 cut(s) 850
StyD4I CCNGG 1 cut(s) 572
TaaI ACNGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 844
TatI WGTACW 1 cut(s) 520
TauI GCSGC 1 cut(s) 49
TfiI GAWTC 5 cut(s) 97, 341, 830, 841, 860
Tru1I TTAA 3 cut(s) 125, 528, 674
Tru9I TTAA 3 cut(s) 125, 528, 674
TscAI CASTG 2 cut(s) 196, 619
TseI GCWGC 1 cut(s) 603
TspDTI ATGAA 6 cut(s) 17, 218, 387, 616, 706, 757
TspRI CASTG 2 cut(s) 196, 619
Tth111I GACNNNGTC 1 cut(s) 422
XapI RAATTY 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 840
Zsp2I ATGCAT 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.