Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0005450

Physical Location & Seq

Genomic Coordinates Forward (+)

40863875 .. 40869119

Gene Structure 5245 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG242700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2643 bp

ATGGAAGCTAACTACGCTAACAACCACCACCACAACCAAGCGACGCGGTGCTTCGGGCCGCAGATGAAAATGACGATTCAGCCCTCGCAGCAGCAGCAGCAGCAGCACTCCGACAACGACCGCAGCAGCGGCGAGCTCCGCGCCCTCGACTGCAACCTCACCTCCCTCTGCGACCACATCCAGACCGAAGGCTTCAACTCCGGCGCCTTCTCCGACGTCCTCGTCCTCGCCATGGGCTCCACCTACCACCTCCACCGCCTCATCCTCTCCCGCAGCCCCTACTTCAGGAATATGCTTCATGGACCTTGGAAAGAAGCCAACGCTCCCATTGTGACATTACATGTAGATGATAAGAATATTAATGCAGAGGCAATTGCAACGGCCTTGGCGTACTTGTATGGCCACCACCCCAAGCTTAGTGATACCAATGCCTTTCGCGTTCTTGCTGCTGCTTCTTTTCTTGATCTTCAGGATTTATGTGCGATATGCACTGACTTCATTATATCGGAACTATGGACTTCAAACTTCTTAGCCTATCAGGTGTTTGCGGAGGGGCAAGATTATGGCATACATGGAGAACGGGTAAGAAATGCTTGCTGGGGCTACCTTTGTCAAAGCGGTGCCATGGAGTTGAAAGAGGTGCTTCCGAGACTTTCTTCTCAAACGTTGCTTGCATTACTCACCTCTGACGAGCTCTGGGTACCCAGTGAAGAGAAACGGTTTGAGCTGGCATTGTGCACATTCCTTTCAAAGGGTGCTCAATGCAAACAGGAAGATTATGATCATGGAAGTTCCAGTTCGGAGACAGGGACGGATGCACATTCCGATTCCTCTAAATCAAATGGAAAGAATCTGACTGATAGCTTCACTAATAAAAGGTTGGAGTCTGAATTGGGGCGCTTAACTCTAAAAGATGATTTAGAGGGACATAATACAGCTCGTAACCTTCTGATAGAGCTTGCAGACTGTGTAGTTGACTTCCAAACAGGAGGTTCAAATGCCAAACAGCAAGTGCAACAAGTTGCTTACCCTCAGTCAAATTTTGAGCCAGGATACAACTGCAGCATGGGAGGTCCTTCATCATTTAATACATTTTCCGACATAGATGCAATTAGAACTTCATGTTATGCAGAAATGCCTATTGGTGTTGGAGTAAGTAGATTGGGGGAAAATGGAGGGGCTATGGAGGGGCCATCTGATGAAGGGTCATGCTACCATTTAAATAACAATAGTTGGCTTGGTAGGGACCAGTCAAGGCAATGCTCGTCAATGAACTCTTCAAGTAGTGAGCTAATGCCAAATGATTGGGGAAGATGTGGCATGCCTCCTCTTTCCTGGGGTGGCAGGGTTGTAGGCAGAAGACAGCTTAAAGGTTATGGTAAAGGAGAACTTGGGGTTGGTGGGGAGGAATATGATGCATTTGTAAATATATTTGAAGGAGGATCTCTTCTATACTGCAACATGTCTTTTGAGGCACTTTTGAATGTAAGAAAGCAACTTGAGGAAATGGGGTTTCCTTGCAAAGCTGTAAATGATGGTCTTTGGCTGCAGATGCTTTTGAGCCAGAGAGTTCAAGAAATTGGCGCTGATACATGCAAAAGTTGCTGTCTAACAAGCATAGCATGCAGTTGCAGACAGCAATTTTCGTTCTCACATGGAGGTACCAACGGATACTACATGCAAGAGCATAATCAGAGTAACTCTTCTGGAGTATACATCACTGAGTCTGCTCCAGGTGAAGGAAATGGCCTCTTCAGGCCTGTACGTGTGCATGTCAGGGGTCCTATTGATGGGCTTGCTGGTATTGGACGTGGAACCACATTTGTCCCAGCAGCCGCTTGGCCTCCAACTCGGTTCGTCTTTTCTCGTGTGCCATTTGGTATGGGCAACAGAAATTGCCAGCAGTCTCTTGCTAATGATGATTCAGAGGCTAGAGCTGACCACACTGCAGAGCTGTCAGGAGATGGATTGACAGCTTTGGTTGGGCTAAGCCAAGGAGGTAACAATGTAGCTAATGCTCATGTAGAGCAAACAGAGAGGGGCTATGAGATGGATATGCAAAGCCGAATGCCTGGAACTTCCATGTCAGTGCCAAGTTCTAGTGGTGCACCCGTGCAGATGGTAGAGTCATCAGACCAAGCACTTGGGATTGAGTGGGATAATGCAAACAGTTCCTCCATATCATTGGATATGAAAACACCTTTAAGTCACTTCCCTCCATTCCGCTTTGGGGTGCAATTTGAGGATGTACACAGACTCAGTGATGGCCAAGTCAAGCACTCTTCAGAAGTCTTTTATGCTGGTTCTTTATGGAAGGTTAGTGTTCAGGCTTTTAATGATGAGGACCCTCAAGGACGCCGAACACTTGGATTATTTCTCCACCGACGTAAAGCTGAGATTACCGACCCGTATAGAAAGGTGCAGATGTATGTCGATTCTCGTGAAAAGGTTACTGCCCGCTACCAGCTAATATGTCCATCAAAGAGGGAAGTCATGGTGTTTGGAAGCTACAAGCAAACAGGCACCCTTTTACCAAAAGCTCCGAAGGGCTGGGGCTGGCGAACAGCTCTGTTATTTGATGAACTTGCAGATCTTCTTCAAAATGGGGCCTTAAGAGTGGCTGCAGTGGTGCAGCTTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0006355 GO:0007276 GO:0007283 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0012505 GO:0016363 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0031323 GO:0031326 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032504 GO:0032991 GO:0034399 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0048232 GO:0048609 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051704 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB

Protein Analysis

880

Amino Acids

96.82

Weight (kDa)

5.57

Isoelectric Point (pI)

48.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	65 - 169	9.1e-15	BTB/POZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15840 AT4G15840
fragaria_vesca	FvH4_4g18240 FvH4_4g18240 FvH4_4g18240 FvH4_4g18240 FvH4_4g18240 FvH4_4g18240 FvH4_4g18240
malus_domestica	MD08G1066800.v1.1 MD13G1167900.v1.1
prunus_persica	Prupe.1G135400_v2.0.a1 Prupe.1G135400_v2.0.a1 Prupe.1G135400_v2.0.a1 Prupe.1G135400_v2.0.a1
pyrus_communis	pycom13g14360
rosa_chinensis	RchiOBHm_Chr4g0422381
rosa_laevigata	RLG00000007614
rosa_multiflora	Rmu_sc0013245.1_g000001 Rmu_sc0034689.1_g000001
rosa_roxburghii	Rroxscaffold_5G00364500
rosa_rugosa	Rorug04G0179600 Rorug04G0179700
rosa_samantha	Rh4AG239100 Rh4AG239500 Rh4BG242700 Rh4CG254100 Rh4DG238200
rosa_wichuraiana	Rw4G020670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	221
AatII	GACGTC	1 cut(s)	219
Acc65I	GGTACC	2 cut(s)	700, 1661
AccB1I	GGYRCC	5 cut(s)	203, 620, 700, 1661, 2522
AccI	GTMKAC	1 cut(s)	1713
AccII	CGCG	3 cut(s)	46, 141, 438
AclI	AACGTT	1 cut(s)	665
AclWI	GGATC	1 cut(s)	1450
AcoI	YGGCCR	2 cut(s)	400, 2266
AcsI	RAATTY	1 cut(s)	1039
AcuI	CTGAAG	4 cut(s)	268, 452, 1738, 2268
AcyI	GRCGYC	3 cut(s)	204, 216, 2356
AfaI	GTAC	5 cut(s)	392, 702, 1663, 1764, 2250
AfiI	CCNNNNNNNGG	6 cut(s)	232, 285, 751, 1739, 1790, 2230
AflII	CTTAAG	1 cut(s)	2611
AflIII	ACRYGT	3 cut(s)	340, 1461, 1765
AjiI	CACGTC	1 cut(s)	1811
AjnI	CCWGG	4 cut(s)	1048, 1334, 1732, 2071
AjuI	GAANNNNNNNTTGG	4 cut(s)	1125, 1157, 1380, 1412
Alw21I	GWGCWC	5 cut(s)	138, 696, 740, 760, 2110
Alw26I	GTCTC	3 cut(s)	643, 797, 1911
Alw44I	GTGCAC	2 cut(s)	736, 2106
AlwI	GGATC	1 cut(s)	1450
AoxI	GGCC	9 cut(s)	56, 381, 400, 1190, 1747, 1757, 1841, 2266, 2607
ApaLI	GTGCAC	2 cut(s)	736, 2106
ApoI	RAATTY	1 cut(s)	1039
AseI	ATTAAT	1 cut(s)	360
Asp718I	GGTACC	2 cut(s)	700, 1661
AspLEI	GCGC	4 cut(s)	143, 206, 900, 1586
AspS9I	GGNCC	8 cut(s)	56, 302, 1073, 1190, 1246, 1781, 2344, 2607
AsuHPI	GGTGA	3 cut(s)	151, 673, 1748
AvaII	GGWCC	5 cut(s)	302, 1073, 1246, 1781, 2344
BaeGI	GKGCMC	2 cut(s)	740, 2110
BalI	TGGCCA	2 cut(s)	402, 2268
BanI	GGYRCC	5 cut(s)	203, 620, 700, 1661, 2522
BanII	GRGCYC	3 cut(s)	138, 239, 696
BauI	CACGAG	2 cut(s)	1866, 2439
BbsI	GAAGAC	1 cut(s)	1366
Bbv12I	GWGCWC	5 cut(s)	138, 696, 740, 760, 2110
BccI	CCATC	8 cut(s)	1201, 1529, 1784, 1958, 2044, 2113, 2258, 2485
BceAI	ACGGC	1 cut(s)	396
BcgI	CGANNNNNNTGC	2 cut(s)	1088, 1122
BciT130I	CCWGG	4 cut(s)	1050, 1336, 1734, 2073
BciVI	GTATCC	2 cut(s)	1046, 1664
BclI	TGATCA	1 cut(s)	781
BcoDI	GTCTC	3 cut(s)	643, 797, 1911
BfaI	CTAG	2 cut(s)	1932, 2100
BfmI	CTRYAG	4 cut(s)	1060, 1547, 1947, 2622
BfoI	RGCGCY	3 cut(s)	207, 901, 1587
BfrI	CTTAAG	1 cut(s)	2611
BfuI	GTATCC	2 cut(s)	1046, 1664
BglII	AGATCT	1 cut(s)	2590
BlpI	GCTNAGC	1 cut(s)	1988
Bme1390I	CCNGG	4 cut(s)	1050, 1336, 1734, 2073
Bme18I	GGWCC	5 cut(s)	302, 1073, 1246, 1781, 2344
BmgBI	CACGTC	1 cut(s)	1811
BmgT120I	GGNCC	8 cut(s)	56, 302, 1073, 1190, 1246, 1781, 2344, 2607
BmrFI	CCNGG	4 cut(s)	1050, 1336, 1734, 2073
BmrI	ACTGGG	1 cut(s)	699
BmsI	GCATC	4 cut(s)	805, 1096, 1405, 1542
BmuI	ACTGGG	1 cut(s)	699
BpiI	GAAGAC	1 cut(s)	1366
BpmI	CTGGAG	2 cut(s)	1716, 1728
Bpu1102I	GCTNAGC	1 cut(s)	1988
BpuEI	CTTGAG	2 cut(s)	1520, 2334
BsaAI	YACGTR	1 cut(s)	1766
BsaBI	GATNNNNATC	1 cut(s)	780
BsaHI	GRCGYC	3 cut(s)	204, 216, 2356
BsaJI	CCNNGG	6 cut(s)	231, 305, 384, 624, 1335, 1993
BsaXI	ACNNNNNCTCC	6 cut(s)	146, 176, 567, 597, 2159, 2189
Bsc4I	CCNNNNNNNGG	6 cut(s)	232, 285, 751, 1739, 1790, 2230
Bse1I	ACTGG	3 cut(s)	705, 795, 1249
Bse3DI	GCAATG	1 cut(s)	1265
Bse8I	GATNNNNATC	1 cut(s)	780
BseBI	CCWGG	4 cut(s)	1050, 1336, 1734, 2073
BseDI	CCNNGG	6 cut(s)	231, 305, 384, 624, 1335, 1993
BseGI	GGATG	4 cut(s)	177, 261, 820, 2251
BseJI	GATNNNNATC	1 cut(s)	780
BseLI	CCNNNNNNNGG	6 cut(s)	232, 285, 751, 1739, 1790, 2230
BseMI	GCAATG	1 cut(s)	1265
BseMII	CTCAG	4 cut(s)	1046, 1713, 2272, 2385
BseNI	ACTGG	3 cut(s)	705, 795, 1249
BseRI	GAGGAG	1 cut(s)	1317
BseSI	GKGCMC	2 cut(s)	740, 2110
BseYI	CCCAGC	3 cut(s)	597, 1828, 2550
BsgI	GTGCAG	2 cut(s)	2135, 2441
Bsh1236I	CGCG	3 cut(s)	46, 141, 438
Bsh1285I	CGRYCG	1 cut(s)	121
BshFI	GGCC	9 cut(s)	58, 383, 402, 1192, 1749, 1759, 1843, 2268, 2609
BshNI	GGYRCC	5 cut(s)	203, 620, 700, 1661, 2522
BsiEI	CGRYCG	1 cut(s)	121
BsiHKAI	GWGCWC	5 cut(s)	138, 696, 740, 760, 2110
BsiSI	CCGG	1 cut(s)	201
BslFI	GGGAC	4 cut(s)	823, 939, 1259, 1811
BslI	CCNNNNNNNGG	6 cut(s)	232, 285, 751, 1739, 1790, 2230
BsmAI	GTCTC	3 cut(s)	643, 797, 1911
BsmFI	GGGAC	4 cut(s)	823, 939, 1259, 1811
BsmI	GAATGC	1 cut(s)	2073
BsnI	GGCC	9 cut(s)	58, 383, 402, 1192, 1749, 1759, 1843, 2268, 2609
Bsp1286I	GDGCHC	6 cut(s)	138, 239, 696, 740, 760, 2110
Bsp1407I	TGTACA	1 cut(s)	2248
Bsp143I	GATC	4 cut(s)	463, 781, 1442, 2590
Bsp1720I	GCTNAGC	1 cut(s)	1988
Bsp19I	CCATGG	2 cut(s)	231, 624
BspANI	GGCC	9 cut(s)	58, 383, 402, 1192, 1749, 1759, 1843, 2268, 2609
BspCNI	CTCAG	4 cut(s)	1045, 1714, 2271, 2386
BspFNI	CGCG	3 cut(s)	46, 141, 438
BspMAI	CTGCAG	4 cut(s)	1064, 1551, 1951, 2626
BspPI	GGATC	1 cut(s)	1450
BspT107I	GGYRCC	5 cut(s)	203, 620, 700, 1661, 2522
BspTI	CTTAAG	1 cut(s)	2611
BsrDI	GCAATG	1 cut(s)	1265
BsrGI	TGTACA	1 cut(s)	2248
BsrI	ACTGG	3 cut(s)	705, 795, 1249
BssECI	CCNNGG	6 cut(s)	231, 305, 384, 624, 1335, 1993
BssMI	GATC	4 cut(s)	463, 781, 1442, 2590
BssNAI	GTATAC	1 cut(s)	1714
BssNI	GRCGYC	3 cut(s)	204, 216, 2356
BssSI	CACGAG	2 cut(s)	1866, 2439
BssT1I	CCWWGG	5 cut(s)	231, 305, 384, 624, 1993
Bst1107I	GTATAC	1 cut(s)	1714
Bst2BI	CACGAG	2 cut(s)	1866, 2439
Bst2UI	CCWGG	4 cut(s)	1050, 1336, 1734, 2073
Bst4CI	ACNGT	3 cut(s)	720, 968, 2171
Bst6I	CTCTTC	6 cut(s)	705, 1282, 1452, 1708, 1757, 2287
BstACI	GRCGYC	3 cut(s)	204, 216, 2356
BstAFI	CTTAAG	1 cut(s)	2611
BstAPI	GCANNNNNTGC	2 cut(s)	1602, 1623
BstAUI	TGTACA	1 cut(s)	2248
BstBAI	YACGTR	1 cut(s)	1766
BstDEI	CTNAG	7 cut(s)	416, 529, 1032, 1722, 1988, 2258, 2394
BstDSI	CCRYGG	2 cut(s)	231, 624
BstENI	CCTNNNNNAGG	2 cut(s)	283, 749
BstF5I	GGATG	4 cut(s)	177, 261, 820, 2251
BstFNI	CGCG	3 cut(s)	46, 141, 438
BstH2I	RGCGCY	3 cut(s)	207, 901, 1587
BstHHI	GCGC	4 cut(s)	143, 206, 900, 1586
BstKTI	GATC	4 cut(s)	466, 784, 1445, 2593
BstMAI	GTCTC	3 cut(s)	643, 797, 1911
BstMBI	GATC	4 cut(s)	463, 781, 1442, 2590
BstMCI	CGRYCG	1 cut(s)	121
BstNI	CCWGG	4 cut(s)	1050, 1336, 1734, 2073
BstNSI	RCATGY	7 cut(s)	344, 1324, 1465, 1596, 1626, 1681, 1775
BstSCI	CCNGG	4 cut(s)	1048, 1334, 1732, 2071
BstSFI	CTRYAG	4 cut(s)	1060, 1547, 1947, 2622
BstSLI	GKGCMC	2 cut(s)	740, 2110
BstUI	CGCG	3 cut(s)	46, 141, 438
BstV2I	GAAGAC	1 cut(s)	1366
BstX2I	RGATCY	2 cut(s)	1442, 2590
BstXI	CCANNNNNNTGG	3 cut(s)	1305, 2144, 2185
BstYI	RGATCY	2 cut(s)	1442, 2590
BstZ17I	GTATAC	1 cut(s)	1714
BsuI	GTATCC	2 cut(s)	1046, 1664
BsuRI	GGCC	9 cut(s)	58, 383, 402, 1192, 1749, 1759, 1843, 2268, 2609
BtgI	CCRYGG	2 cut(s)	231, 624
BtrI	CACGTC	1 cut(s)	1811
BtsCI	GGATG	4 cut(s)	177, 261, 820, 2251
BtsI	GCAGTG	2 cut(s)	1944, 2631
BtsIMutI	CAGTG	7 cut(s)	489, 712, 1719, 1944, 2094, 2266, 2631
CfoI	GCGC	4 cut(s)	143, 206, 900, 1586
Cfr13I	GGNCC	8 cut(s)	56, 302, 1073, 1190, 1246, 1781, 2344, 2607
CseI	GACGC	2 cut(s)	52, 2364
Csp6I	GTAC	5 cut(s)	391, 701, 1662, 1763, 2249
CviQI	GTAC	5 cut(s)	391, 701, 1662, 1763, 2249
DdeI	CTNAG	7 cut(s)	416, 529, 1032, 1722, 1988, 2258, 2394
DinI	GGCGCC	1 cut(s)	205
DpnI	GATC	4 cut(s)	465, 783, 1444, 2592
DpnII	GATC	4 cut(s)	463, 781, 1442, 2590
DraI	TTTAAA	1 cut(s)	1221
DrdI	GACNNNNNNGTC	1 cut(s)	221
DseDI	GACNNNNNNGTC	1 cut(s)	221
EaeI	YGGCCR	2 cut(s)	400, 2266
Eam1104I	CTCTTC	6 cut(s)	705, 1282, 1452, 1708, 1757, 2287
EarI	CTCTTC	6 cut(s)	705, 1282, 1452, 1708, 1757, 2287
Ecl136II	GAGCTC	2 cut(s)	136, 694
Eco130I	CCWWGG	5 cut(s)	231, 305, 384, 624, 1993
Eco147I	AGGCCT	1 cut(s)	1759
Eco24I	GRGCYC	3 cut(s)	138, 239, 696
Eco47I	GGWCC	5 cut(s)	302, 1073, 1246, 1781, 2344
Eco53kI	GAGCTC	2 cut(s)	136, 694
Eco57I	CTGAAG	4 cut(s)	268, 452, 1738, 2268
EcoICRI	GAGCTC	2 cut(s)	136, 694
EcoNI	CCTNNNNNAGG	2 cut(s)	283, 749
EcoO109I	RGGNCCY	4 cut(s)	1073, 1781, 2344, 2607
EcoRII	CCWGG	4 cut(s)	1048, 1334, 1732, 2071
EcoT14I	CCWWGG	5 cut(s)	231, 305, 384, 624, 1993
EcoT22I	ATGCAT	1 cut(s)	1420
EcoT38I	GRGCYC	3 cut(s)	138, 239, 696
EgeI	GGCGCC	1 cut(s)	205
EheI	GGCGCC	1 cut(s)	205
ErhI	CCWWGG	5 cut(s)	231, 305, 384, 624, 1993
FalI	AAGNNNNNCTT	6 cut(s)	577, 609, 627, 659, 1374, 1406
FaqI	GGGAC	4 cut(s)	823, 939, 1259, 1811
FauI	CCCGC	2 cut(s)	278, 2465
FbaI	TGATCA	1 cut(s)	781
FblI	GTMKAC	1 cut(s)	1713
FokI	GGATG	4 cut(s)	164, 248, 827, 2258
FriOI	GRGCYC	3 cut(s)	138, 239, 696
FspBI	CTAG	2 cut(s)	1932, 2100
GlaI	GCGC	4 cut(s)	142, 205, 899, 1585
GsaI	CCCAGC	3 cut(s)	601, 1832, 2554
GsuI	CTGGAG	2 cut(s)	1716, 1728
HaeII	RGCGCY	3 cut(s)	207, 901, 1587
HaeIII	GGCC	9 cut(s)	58, 383, 402, 1192, 1749, 1759, 1843, 2268, 2609
HapII	CCGG	1 cut(s)	201
HgaI	GACGC	2 cut(s)	52, 2364
HhaI	GCGC	4 cut(s)	143, 206, 900, 1586
Hin1I	GRCGYC	3 cut(s)	204, 216, 2356
Hin6I	GCGC	4 cut(s)	141, 204, 898, 1584
HinP1I	GCGC	4 cut(s)	141, 204, 898, 1584
HincII	GTYRAC	1 cut(s)	976
HindII	GTYRAC	1 cut(s)	976
HindIII	AAGCTT	1 cut(s)	413
HinfI	GANTC	9 cut(s)	76, 827, 850, 884, 1724, 1922, 2126, 2256, 2435
HpaII	CCGG	1 cut(s)	201
HphI	GGTGA	3 cut(s)	151, 673, 1748
Hpy166II	GTNNAC	5 cut(s)	738, 976, 1714, 2108, 2251
Hpy188III	TCNNGA	8 cut(s)	181, 286, 461, 470, 1574, 1707, 1959, 2441
Hpy8I	GTNNAC	5 cut(s)	738, 976, 1714, 2108, 2251
Hpy99I	CGWCG	3 cut(s)	46, 218, 2388
HpyAV	CCTTC	9 cut(s)	182, 217, 956, 1086, 1196, 1430, 1733, 2308, 2539
HpyCH4III	ACNGT	3 cut(s)	720, 968, 2171
HpyCH4IV	ACGT	5 cut(s)	216, 665, 1765, 1810, 2386
HpyF3I	CTNAG	7 cut(s)	416, 529, 1032, 1722, 1988, 2258, 2394
HpySE526I	ACGT	5 cut(s)	216, 665, 1765, 1810, 2386
Hsp92I	GRCGYC	3 cut(s)	204, 216, 2356
HspAI	GCGC	4 cut(s)	141, 204, 898, 1584
KasI	GGCGCC	1 cut(s)	203
KpnI	GGTACC	2 cut(s)	704, 1665
Ksp22I	TGATCA	1 cut(s)	781
Kzo9I	GATC	4 cut(s)	463, 781, 1442, 2590
LmnI	GCTCC	5 cut(s)	141, 242, 328, 1735, 2545
LweI	GCATC	4 cut(s)	805, 1096, 1405, 1542
MaeI	CTAG	2 cut(s)	1932, 2100
MaeII	ACGT	5 cut(s)	216, 665, 1765, 1810, 2386
MaeIII	GTNAC	6 cut(s)	331, 941, 1697, 2000, 2207, 2449
MalI	GATC	4 cut(s)	465, 783, 1444, 2592
MboI	GATC	4 cut(s)	463, 781, 1442, 2590
MfeI	CAATTG	1 cut(s)	372
MflI	RGATCY	2 cut(s)	1442, 2590
MhlI	GDGCHC	6 cut(s)	138, 239, 696, 740, 760, 2110
MlsI	TGGCCA	2 cut(s)	402, 2268
MluCI	AATT	8 cut(s)	372, 890, 1039, 1110, 1578, 1640, 1894, 2237
MluNI	TGGCCA	2 cut(s)	402, 2268
Mly113I	GGCGCC	1 cut(s)	204
MlyI	GAGTC	4 cut(s)	893, 1733, 2135, 2250
MmeI	TCCRAC	6 cut(s)	135, 237, 861, 1122, 1129, 1871
Mox20I	TGGCCA	2 cut(s)	402, 2268
Mph1103I	ATGCAT	1 cut(s)	1420
MscI	TGGCCA	2 cut(s)	402, 2268
MseI	TTAA	8 cut(s)	360, 902, 1086, 1220, 1368, 2204, 2334, 2612
MslI	CAYNNNNRTG	2 cut(s)	345, 2087
Msp20I	TGGCCA	2 cut(s)	402, 2268
MspA1I	CMGCKG	1 cut(s)	129
MspCI	CTTAAG	1 cut(s)	2611
MspI	CCGG	1 cut(s)	201
MspR9I	CCNGG	4 cut(s)	1050, 1336, 1734, 2073
MunI	CAATTG	1 cut(s)	372
Mva1269I	GAATGC	1 cut(s)	2073
MvaI	CCWGG	4 cut(s)	1050, 1336, 1734, 2073
MvnI	CGCG	3 cut(s)	46, 141, 438
NarI	GGCGCC	1 cut(s)	204
NcoI	CCATGG	2 cut(s)	231, 624
NdeII	GATC	4 cut(s)	463, 781, 1442, 2590
NmuCI	GTSAC	2 cut(s)	331, 2207
NsiI	ATGCAT	1 cut(s)	1420
NspI	RCATGY	7 cut(s)	344, 1324, 1465, 1596, 1626, 1681, 1775
PaeI	GCATGC	2 cut(s)	1324, 1626
PceI	AGGCCT	1 cut(s)	1759
PciI	ACATGT	2 cut(s)	340, 1461
PcsI	WCGNNNNNNNCGW	1 cut(s)	386
PctI	GAATGC	1 cut(s)	2073
PfeI	GAWTC	5 cut(s)	76, 827, 850, 1922, 2435
PleI	GAGTC	4 cut(s)	892, 1732, 2134, 2250
PluTI	GGCGCC	1 cut(s)	207
PpsI	GAGTC	4 cut(s)	892, 1732, 2134, 2250
Ppu21I	YACGTR	1 cut(s)	1766
PpuMI	RGGWCCY	3 cut(s)	1073, 1781, 2344
PscI	ACATGT	2 cut(s)	340, 1461
PshBI	ATTAAT	1 cut(s)	360
Psp124BI	GAGCTC	2 cut(s)	138, 696
Psp1406I	AACGTT	1 cut(s)	665
Psp5II	RGGWCCY	3 cut(s)	1073, 1781, 2344
Psp6I	CCWGG	4 cut(s)	1048, 1334, 1732, 2071
PspFI	CCCAGC	3 cut(s)	597, 1828, 2550
PspGI	CCWGG	4 cut(s)	1048, 1334, 1732, 2071
PspPI	GGNCC	8 cut(s)	56, 302, 1073, 1190, 1246, 1781, 2344, 2607
PspPPI	RGGWCCY	3 cut(s)	1073, 1781, 2344
PstI	CTGCAG	4 cut(s)	1064, 1551, 1951, 2626
PsuI	RGATCY	2 cut(s)	1442, 2590
RsaI	GTAC	5 cut(s)	392, 702, 1663, 1764, 2250
RsaNI	GTAC	5 cut(s)	391, 701, 1662, 1763, 2249
RseI	CAYNNNNRTG	2 cut(s)	345, 2087
SacI	GAGCTC	2 cut(s)	138, 696
SaqAI	TTAA	8 cut(s)	360, 902, 1086, 1220, 1368, 2204, 2334, 2612
Sau3AI	GATC	4 cut(s)	463, 781, 1442, 2590
Sau96I	GGNCC	8 cut(s)	56, 302, 1073, 1190, 1246, 1781, 2344, 2607
SchI	GAGTC	4 cut(s)	893, 1733, 2135, 2250
ScrFI	CCNGG	4 cut(s)	1050, 1336, 1734, 2073
SduI	GDGCHC	6 cut(s)	138, 239, 696, 740, 760, 2110
SfaNI	GCATC	4 cut(s)	805, 1096, 1405, 1542
SfcI	CTRYAG	4 cut(s)	1060, 1547, 1947, 2622
SfoI	GGCGCC	1 cut(s)	205
SinI	GGWCC	5 cut(s)	302, 1073, 1246, 1781, 2344
SmiI	ATTTAAAT	1 cut(s)	1221
SmiMI	CAYNNNNRTG	2 cut(s)	345, 2087
SmlI	CTYRAG	3 cut(s)	1499, 2349, 2611
SmoI	CTYRAG	3 cut(s)	1499, 2349, 2611
SphI	GCATGC	2 cut(s)	1324, 1626
Sse9I	AATT	8 cut(s)	372, 890, 1039, 1110, 1578, 1640, 1894, 2237
SseBI	AGGCCT	1 cut(s)	1759
SspDI	GGCGCC	1 cut(s)	203
SspI	AATATT	1 cut(s)	358
SspMI	CTAG	2 cut(s)	1932, 2100
SstI	GAGCTC	2 cut(s)	138, 696
StuI	AGGCCT	1 cut(s)	1759
StyD4I	CCNGG	4 cut(s)	1048, 1334, 1732, 2071
StyI	CCWWGG	5 cut(s)	231, 305, 384, 624, 1993
SwaI	ATTTAAAT	1 cut(s)	1221
TaaI	ACNGT	3 cut(s)	720, 968, 2171
TaiI	ACGT	5 cut(s)	219, 668, 1768, 1813, 2389
TaqI	TCGA	2 cut(s)	147, 2433
TaqII	GACCGA	1 cut(s)	200
TasI	AATT	8 cut(s)	372, 890, 1039, 1110, 1578, 1640, 1894, 2237
TatI	WGTACW	1 cut(s)	2248
TauI	GCSGC	3 cut(s)	61, 132, 1838
TfiI	GAWTC	5 cut(s)	76, 827, 850, 1922, 2435
Tru1I	TTAA	8 cut(s)	360, 902, 1086, 1220, 1368, 2204, 2334, 2612
Tru9I	TTAA	8 cut(s)	360, 902, 1086, 1220, 1368, 2204, 2334, 2612
TscAI	CASTG	7 cut(s)	496, 712, 1726, 1951, 2094, 2266, 2631
TseFI	GTSAC	2 cut(s)	331, 2207
Tsp45I	GTSAC	2 cut(s)	331, 2207
TspDTI	ATGAA	9 cut(s)	80, 287, 487, 1068, 1110, 1215, 1286, 2207, 2595
TspGWI	ACGGA	2 cut(s)	827, 1683
TspRI	CASTG	7 cut(s)	496, 712, 1726, 1951, 2094, 2266, 2631
Vha464I	CTTAAG	1 cut(s)	2611
VneI	GTGCAC	2 cut(s)	736, 2106
VpaK11BI	GGWCC	5 cut(s)	302, 1073, 1246, 1781, 2344
VspI	ATTAAT	1 cut(s)	360
XagI	CCTNNNNNAGG	2 cut(s)	283, 749
XapI	RAATTY	1 cut(s)	1039
XceI	RCATGY	7 cut(s)	344, 1324, 1465, 1596, 1626, 1681, 1775
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1836
XmiI	GTMKAC	1 cut(s)	1713
XspI	CTAG	2 cut(s)	1932, 2100
ZraI	GACGTC	1 cut(s)	217
Zsp2I	ATGCAT	1 cut(s)	1420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.