Rh4DG367900

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004356
Physical Location & Seq
Reverse (-)
58741813 .. 58743856
2044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG367900.1

Sequence Viewer

Length: 1353 bp
ATGAGAAGCTCCTGGTTGAGTAAACTCTCTCTCATTTTTGGCCCTAGACCACCCCTCAACTGGTTACTCTTGTGCCTCGTGAGTGTACTTGCACTAATTGCAGTGTTGGGATCATCATCTTCAAGTACATTTGATTCTTTAACCCCTAGTTCAGTACCAGACATCTACACAAACTATAGGAGGCTAAAAGAGCAAGCGGCAGTTGATTATTTGGAGCTGAGGTCTCTCTCACTGGGGGGTAGTAGAGAAAAAGAGCTTGGCCTTTGTGGCAGAGATAAAGAACACTCTGTGCCTTGTTACAATGTTTCAGCAAATCTTTTAGCCGGGTATAAAGATGGGGAGGAGTTTGATCGGCATTGTGAAGTATCAAGAGACAGAGAACAGTGTCTAGTTCGCCCCCCTAAGGACTATAAGATCCCTCTGAGGTGGCCAGCTGGTAGGGACGTGATATGGAGTGGAAACGTGAAGATAACCAAAGACCAATTTCTGTCATCTGGAAGCATGACCAAAAGGTTGATGTTACTAGAAGAAAATCAAATTTCCTTCCACTCTGAGGATGGTTTGATTTTTGATGGTGTCAAGGATTATTCTCGTCAAATTGCTGAGATGCTGGGTTTGAGCAGTGACTCTGAATTTCCTCAAGCTGGTGTTCACACAGTACTAGATATTGGTTGTGGTTTTGGTAGCTTTGGCGCTCATTTAGTATCCCTAAAGTTAATGGCTATTTGTATTGCGGCATATGAGGCAACTGGCAGTCAAGTTCAGTTGGCCCTTGAGAGAGGTCTTCCAGCAATGATCGGCAACTTCATCACAAGACAACTTCCATATCCATCTCTGTCATTTGAAATGGTTCATTGTGCTCAATGTGGTATTCTTTGGGACAAAAAAGATGGGATGTTTCTTTTAGAAGTTGACCGGGTACTCAAGCCTGGAGGTTACTTTGTTTTAACCTCACCCACAAGCCAGCCATATGGAAGTTCATCGACTATGAAGGAGGGCATAGCTACAGACTCTTCACTAATGGAAGAATTAACCCAGAAAATCTGTTGGACTCTTTTGGCTCAAAAATATGAAACTTTTATCTGGCAGAAAACTGTGGATTCTAATTGCTATACATCTCGCAAGCAGGGTGCTATTCCACTTTGTCAAGAAGAGCATGAGGTTCAGTCATATTATAAGCCCCTAGTATCGTGTATAAGTGGGACCATGAGCAAACGCTGGACTCCAATCCGAAATAGGTCTAGTTCCCAGTGGAACTCATCTAAGCTTGAAGTTCATGGAAAGTATTGTTCCATCGTACATTTGTTTTTCTTCACTTCTCTGTCTCTCTTCGCTGGAAGGGGTTGTTTCTGA

Protein Analysis

450

Amino Acids

50.31

Weight (kDa)

7.48

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 97 - 418 8.9e-79 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_31 PF13847 219 - 343 3.2e-06 Methyltransferase domain
Methyltransf_25 PF13649 220 - 312 4.9e-07 Methyltransferase domain
Methyltransf_11 PF08241 221 - 316 6.2e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1176
AciI CCGC 2 cut(s) 197, 734
AclWI GGATC 2 cut(s) 118, 409
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 2 cut(s) 537, 632
AdeI CACNNNGTG 1 cut(s) 289
AfaI GTAC 6 cut(s) 87, 127, 156, 660, 921, 1299
AfiI CCNNNNNNNGG 4 cut(s) 60, 403, 553, 644
AgsI TTSAA 3 cut(s) 123, 845, 1271
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 2 cut(s) 11, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AluBI AGCT 8 cut(s) 9, 217, 256, 434, 644, 687, 1004, 1267
AluI AGCT 8 cut(s) 9, 217, 256, 434, 644, 687, 1004, 1267
Alw21I GWGCWC 1 cut(s) 862
Alw26I GTCTC 3 cut(s) 228, 366, 1329
AlwI GGATC 2 cut(s) 118, 409
AoxI GGCC 4 cut(s) 40, 259, 428, 768
ApoI RAATTY 2 cut(s) 537, 632
Asp700I GAANNNNTTC 1 cut(s) 849
AspLEI GCGC 1 cut(s) 695
AspS9I GGNCC 3 cut(s) 41, 769, 1203
AsuC2I CCSGG 2 cut(s) 325, 917
AsuHPI GGTGA 1 cut(s) 945
AvaII GGWCC 1 cut(s) 1203
AxyI CCTNAGG 1 cut(s) 402
BalI TGGCCA 1 cut(s) 430
BauI CACGAG 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 776
Bbv12I GWGCWC 1 cut(s) 862
BbvCI CCTCAGC 1 cut(s) 218
BccI CCATC 6 cut(s) 329, 551, 566, 838, 884, 1301
BciT130I CCWGG 2 cut(s) 13, 930
BciVI GTATCC 1 cut(s) 715
BcnI CCSGG 2 cut(s) 325, 917
BcoDI GTCTC 3 cut(s) 228, 366, 1329
BfaI CTAG 7 cut(s) 45, 147, 389, 524, 662, 1184, 1242
BfmI CTRYAG 2 cut(s) 175, 1005
BfoI RGCGCY 1 cut(s) 696
BfuI GTATCC 1 cut(s) 715
BglI GCCNNNNNGGC 1 cut(s) 267
BisI GCNGC 2 cut(s) 198, 735
BlsI GCNGC 2 cut(s) 199, 736
BmcAI AGTACT 1 cut(s) 660
Bme1390I CCNGG 4 cut(s) 13, 325, 917, 930
Bme18I GGWCC 1 cut(s) 1203
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 3 cut(s) 41, 769, 1203
BmiI GGNNCC 1 cut(s) 1204
BmrFI CCNGG 4 cut(s) 13, 325, 917, 930
BmrI ACTGGG 2 cut(s) 242, 1243
BmsI GCATC 1 cut(s) 597
BmuI ACTGGG 2 cut(s) 242, 1243
BpiI GAAGAC 1 cut(s) 776
BpmI CTGGAG 1 cut(s) 951
Bpu10I CCTNAGC 1 cut(s) 218
BpuEI CTTGAG 3 cut(s) 624, 794, 908
BpuMI CCSGG 2 cut(s) 325, 917
BsaBI GATNNNNATC 1 cut(s) 115
BsaI GGTCTC 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 403, 553, 644
Bse1I ACTGG 4 cut(s) 65, 237, 754, 1249
Bse21I CCTNAGG 1 cut(s) 402
Bse3DI GCAATG 1 cut(s) 798
Bse8I GATNNNNATC 1 cut(s) 115
BseBI CCWGG 2 cut(s) 13, 930
BseGI GGATG 2 cut(s) 562, 900
BseJI GATNNNNATC 1 cut(s) 115
BseLI CCNNNNNNNGG 4 cut(s) 60, 403, 553, 644
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 4 cut(s) 209, 413, 543, 594
BseNI ACTGG 4 cut(s) 65, 237, 754, 1249
BseRI GAGGAG 1 cut(s) 356
BseYI CCCAGC 1 cut(s) 610
BshFI GGCC 4 cut(s) 42, 261, 430, 770
BsiHKAI GWGCWC 1 cut(s) 862
BsiSI CCGG 2 cut(s) 324, 916
BslFI GGGAC 3 cut(s) 455, 893, 1216
BslI CCNNNNNNNGG 4 cut(s) 60, 403, 553, 644
BsmAI GTCTC 3 cut(s) 228, 366, 1329
BsmFI GGGAC 3 cut(s) 455, 893, 1216
BsnI GGCC 4 cut(s) 42, 261, 430, 770
Bso31I GGTCTC 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 862
Bsp143I GATC 4 cut(s) 110, 349, 414, 795
BspACI CCGC 2 cut(s) 197, 734
BspANI GGCC 4 cut(s) 42, 261, 430, 770
BspCNI CTCAG 4 cut(s) 210, 414, 544, 595
BspLI GGNNCC 1 cut(s) 1204
BspPI GGATC 2 cut(s) 118, 409
BspQI GCTCTTC 1 cut(s) 1146
BspTNI GGTCTC 1 cut(s) 228
BsrDI GCAATG 1 cut(s) 798
BsrI ACTGG 4 cut(s) 65, 237, 754, 1249
BssMI GATC 4 cut(s) 110, 349, 414, 795
BssSI CACGAG 1 cut(s) 77
Bst2BI CACGAG 1 cut(s) 77
Bst2UI CCWGG 2 cut(s) 13, 930
Bst4CI ACNGT 3 cut(s) 384, 658, 1096
Bst6I CTCTTC 3 cut(s) 1018, 1146, 1334
BstAPI GCANNNNNTGC 1 cut(s) 98
BstC8I GCNNGC 4 cut(s) 195, 432, 965, 1124
BstDEI CTNAG 6 cut(s) 218, 402, 422, 552, 603, 1263
BstF5I GGATG 2 cut(s) 562, 900
BstH2I RGCGCY 1 cut(s) 696
BstHHI GCGC 1 cut(s) 695
BstKTI GATC 4 cut(s) 113, 352, 417, 798
BstMAI GTCTC 3 cut(s) 228, 366, 1329
BstMBI GATC 4 cut(s) 110, 349, 414, 795
BstMWI GCNNNNNNNGC 4 cut(s) 98, 190, 267, 743
BstNI CCWGG 2 cut(s) 13, 930
BstSCI CCNGG 4 cut(s) 11, 323, 915, 928
BstSFI CTRYAG 2 cut(s) 175, 1005
BstV2I GAAGAC 1 cut(s) 776
BstX2I RGATCY 1 cut(s) 414
BstXI CCANNNNNNTGG 1 cut(s) 971
BstYI RGATCY 1 cut(s) 414
Bsu36I CCTNAGG 1 cut(s) 402
BsuI GTATCC 1 cut(s) 715
BsuRI GGCC 4 cut(s) 42, 261, 430, 770
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 2 cut(s) 562, 900
BtsI GCAGTG 2 cut(s) 108, 628
BtsIMutI CAGTG 5 cut(s) 108, 230, 389, 628, 1256
Cac8I GCNNGC 4 cut(s) 195, 432, 965, 1124
CfoI GCGC 1 cut(s) 695
Cfr13I GGNCC 3 cut(s) 41, 769, 1203
Csp6I GTAC 6 cut(s) 86, 126, 155, 659, 920, 1298
CviAII CATG 4 cut(s) 502, 1157, 1207, 1277
CviQI GTAC 6 cut(s) 86, 126, 155, 659, 920, 1298
DdeI CTNAG 6 cut(s) 218, 402, 422, 552, 603, 1263
DpnI GATC 4 cut(s) 112, 351, 416, 797
DpnII GATC 4 cut(s) 110, 349, 414, 795
DraIII CACNNNGTG 1 cut(s) 289
EaeI YGGCCR 1 cut(s) 428
Eam1104I CTCTTC 3 cut(s) 1018, 1146, 1334
EarI CTCTTC 3 cut(s) 1018, 1146, 1334
Eco31I GGTCTC 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 1203
Eco81I CCTNAGG 1 cut(s) 402
EcoRII CCWGG 2 cut(s) 11, 928
FaeI CATG 4 cut(s) 505, 1160, 1210, 1280
FaqI GGGAC 3 cut(s) 455, 893, 1216
FatI CATG 4 cut(s) 501, 1156, 1206, 1276
FauNDI CATATG 2 cut(s) 739, 970
Fnu4HI GCNGC 2 cut(s) 198, 735
FokI GGATG 2 cut(s) 569, 907
Fsp4HI GCNGC 2 cut(s) 198, 735
FspBI CTAG 7 cut(s) 45, 147, 389, 524, 662, 1184, 1242
GlaI GCGC 1 cut(s) 694
GluI GCNGC 2 cut(s) 198, 735
GsaI CCCAGC 1 cut(s) 614
GsuI CTGGAG 1 cut(s) 951
HaeII RGCGCY 1 cut(s) 696
HaeIII GGCC 4 cut(s) 42, 261, 430, 770
HapII CCGG 2 cut(s) 324, 916
HhaI GCGC 1 cut(s) 695
Hin1II CATG 4 cut(s) 505, 1160, 1210, 1280
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HincII GTYRAC 1 cut(s) 913
HindII GTYRAC 1 cut(s) 913
HindIII AAGCTT 1 cut(s) 1265
HinfI GANTC 6 cut(s) 134, 626, 1010, 1051, 1100, 1222
HpaII CCGG 2 cut(s) 324, 916
HphI GGTGA 1 cut(s) 945
Hpy166II GTNNAC 4 cut(s) 23, 86, 652, 913
Hpy188I TCNGA 5 cut(s) 423, 553, 631, 1232, 1352
Hpy188III TCNNGA 4 cut(s) 79, 369, 495, 1148
Hpy8I GTNNAC 4 cut(s) 23, 86, 652, 913
HpyAV CCTTC 3 cut(s) 553, 985, 1332
HpyCH4III ACNGT 3 cut(s) 384, 658, 1096
HpyCH4IV ACGT 2 cut(s) 444, 462
HpyCH4V TGCA 2 cut(s) 92, 101
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 190, 267, 743
HpyF3I CTNAG 6 cut(s) 218, 402, 422, 552, 603, 1263
HpySE526I ACGT 2 cut(s) 444, 462
Hsp92II CATG 4 cut(s) 505, 1160, 1210, 1280
HspAI GCGC 1 cut(s) 693
Kzo9I GATC 4 cut(s) 110, 349, 414, 795
LguI GCTCTTC 1 cut(s) 1146
LmnI GCTCC 2 cut(s) 14, 214
LweI GCATC 1 cut(s) 597
MaeI CTAG 7 cut(s) 45, 147, 389, 524, 662, 1184, 1242
MaeII ACGT 2 cut(s) 444, 462
MaeIII GTNAC 5 cut(s) 63, 296, 519, 623, 935
MalI GATC 4 cut(s) 112, 351, 416, 797
MboI GATC 4 cut(s) 110, 349, 414, 795
MboII GAAGA 9 cut(s) 111, 478, 539, 776, 1005, 1037, 1163, 1303, 1321
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 862
MlsI TGGCCA 1 cut(s) 430
MluCI AATT 7 cut(s) 96, 482, 537, 597, 632, 1028, 1105
MluNI TGGCCA 1 cut(s) 430
MlyI GAGTC 4 cut(s) 620, 1004, 1045, 1216
MmeI TCCRAC 1 cut(s) 1028
Mox20I TGGCCA 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 849
MscI TGGCCA 1 cut(s) 430
MseI TTAA 4 cut(s) 140, 716, 947, 1031
Msp20I TGGCCA 1 cut(s) 430
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 2 cut(s) 324, 916
MspR9I CCNGG 4 cut(s) 13, 325, 917, 930
MvaI CCWGG 2 cut(s) 13, 930
MwoI GCNNNNNNNGC 4 cut(s) 98, 190, 267, 743
NciI CCSGG 2 cut(s) 325, 917
NdeI CATATG 2 cut(s) 739, 970
NdeII GATC 4 cut(s) 110, 349, 414, 795
NlaIII CATG 4 cut(s) 505, 1160, 1210, 1280
NlaIV GGNNCC 1 cut(s) 1204
NmuCI GTSAC 1 cut(s) 623
PciSI GCTCTTC 1 cut(s) 1146
PdmI GAANNNNTTC 1 cut(s) 849
PfeI GAWTC 2 cut(s) 134, 1100
PkrI GCNGC 2 cut(s) 199, 736
PleI GAGTC 4 cut(s) 620, 1004, 1045, 1216
PpsI GAGTC 4 cut(s) 620, 1004, 1045, 1216
PsiI TTATAA 1 cut(s) 1176
Psp6I CCWGG 2 cut(s) 11, 928
PspFI CCCAGC 1 cut(s) 610
PspGI CCWGG 2 cut(s) 11, 928
PspN4I GGNNCC 1 cut(s) 1204
PspPI GGNCC 3 cut(s) 41, 769, 1203
PsuI RGATCY 1 cut(s) 414
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 6 cut(s) 87, 127, 156, 660, 921, 1299
RsaNI GTAC 6 cut(s) 86, 126, 155, 659, 920, 1298
SapI GCTCTTC 1 cut(s) 1146
SaqAI TTAA 4 cut(s) 140, 716, 947, 1031
SatI GCNGC 2 cut(s) 198, 735
Sau3AI GATC 4 cut(s) 110, 349, 414, 795
Sau96I GGNCC 3 cut(s) 41, 769, 1203
ScaI AGTACT 1 cut(s) 660
SchI GAGTC 4 cut(s) 620, 1004, 1045, 1216
ScrFI CCNGG 4 cut(s) 13, 325, 917, 930
SduI GDGCHC 1 cut(s) 862
SfaNI GCATC 1 cut(s) 597
SfcI CTRYAG 2 cut(s) 175, 1005
SinI GGWCC 1 cut(s) 1203
SmlI CTYRAG 3 cut(s) 639, 773, 923
SmoI CTYRAG 3 cut(s) 639, 773, 923
Sse9I AATT 7 cut(s) 96, 482, 537, 597, 632, 1028, 1105
SsiI CCGC 2 cut(s) 197, 734
SspMI CTAG 7 cut(s) 45, 147, 389, 524, 662, 1184, 1242
StyD4I CCNGG 4 cut(s) 11, 323, 915, 928
TaaI ACNGT 3 cut(s) 384, 658, 1096
TaiI ACGT 2 cut(s) 447, 465
TaqI TCGA 1 cut(s) 983
TasI AATT 7 cut(s) 96, 482, 537, 597, 632, 1028, 1105
TatI WGTACW 3 cut(s) 85, 125, 658
TauI GCSGC 2 cut(s) 200, 737
TfiI GAWTC 2 cut(s) 134, 1100
Tru1I TTAA 4 cut(s) 140, 716, 947, 1031
Tru9I TTAA 4 cut(s) 140, 716, 947, 1031
TscAI CASTG 5 cut(s) 108, 237, 389, 628, 1256
TseFI GTSAC 1 cut(s) 623
Tsp45I GTSAC 1 cut(s) 623
TspDTI ATGAA 6 cut(s) 796, 842, 969, 1004, 1086, 1265
TspRI CASTG 5 cut(s) 108, 237, 389, 628, 1256
VpaK11BI GGWCC 1 cut(s) 1203
XapI RAATTY 2 cut(s) 537, 632
XcmI CCANNNNNNNNNTGG 2 cut(s) 57, 554
XmnI GAANNNNTTC 1 cut(s) 849
XspI CTAG 7 cut(s) 45, 147, 389, 524, 662, 1184, 1242
ZrmI AGTACT 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.