Rh5BG224000

Belongs to the peptidase M24B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0013744
Physical Location & Seq
Forward (+)
26013078 .. 26019780
6703 bp
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UTR
Exon/CDS
Intron
Rh5BG224000.1

Sequence Viewer

Length: 273 bp
ATGCCTCCTGGATTCCTGTTTGTGATAATTATGTCTACTGTCCGATGTAATGGGAACGGGTCAGTTGGTTTCTTGTCAAACCAGCAAAGAGCAATTGTTGCCTTAGCAAGTAGAGGAAGTGACGTTCCACATTCACCTGTTATGTATGCACATCTGATTGTGGAGATTGACAAAACAAAGTTATTTGTAGATACTTCCAAAGTAGGCATGAGGTGTAGGATCACCTGGCGAATGTGGGTTTCAGGCCATATGACTCCATTCTTTCTGAAGTAG

Protein Analysis

90

Amino Acids

10.08

Weight (kDa)

10.19

Isoelectric Point (pI)

23.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Creatinase_N_2 PF16189 36 - 69 3.1e-08 Creatinase/Prolidase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 35
AclWI GGATC 1 cut(s) 227
AjnI CCWGG 2 cut(s) 7, 224
AloI GAACNNNNNNTCC 2 cut(s) 108, 140
AlwI GGATC 1 cut(s) 227
AoxI GGCC 1 cut(s) 244
AsuHPI GGTGA 2 cut(s) 126, 214
BciT130I CCWGG 2 cut(s) 9, 226
Bme1390I CCNGG 2 cut(s) 9, 226
BmrFI CCNGG 2 cut(s) 9, 226
Bpu10I CCTNAGC 1 cut(s) 103
BseBI CCWGG 2 cut(s) 9, 226
BshFI GGCC 1 cut(s) 246
BsnI GGCC 1 cut(s) 246
Bsp143I GATC 1 cut(s) 219
BspANI GGCC 1 cut(s) 246
BspPI GGATC 1 cut(s) 227
BssMI GATC 1 cut(s) 219
Bst2UI CCWGG 2 cut(s) 9, 226
Bst4CI ACNGT 1 cut(s) 40
BstAPI GCANNNNNTGC 1 cut(s) 98
BstDEI CTNAG 1 cut(s) 103
BstKTI GATC 1 cut(s) 222
BstMBI GATC 1 cut(s) 219
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 2 cut(s) 9, 226
BstSCI CCNGG 2 cut(s) 7, 224
BsuRI GGCC 1 cut(s) 246
CviAII CATG 1 cut(s) 208
CviJI RGCY 1 cut(s) 246
CviKI_1 RGCY 1 cut(s) 246
DdeI CTNAG 1 cut(s) 103
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
EcoRII CCWGG 2 cut(s) 7, 224
FaeI CATG 1 cut(s) 211
FaiI YATR 6 cut(s) 32, 143, 147, 209, 249, 251
FatI CATG 1 cut(s) 207
FauNDI CATATG 1 cut(s) 249
FblI GTMKAC 1 cut(s) 35
HaeIII GGCC 1 cut(s) 246
Hin1II CATG 1 cut(s) 211
HinfI GANTC 2 cut(s) 12, 253
HphI GGTGA 2 cut(s) 126, 214
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 3 cut(s) 44, 156, 267
Hpy8I GTNNAC 1 cut(s) 36
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 1 cut(s) 149
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 1 cut(s) 103
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 1 cut(s) 211
Kzo9I GATC 1 cut(s) 219
LpnPI CCDG 7 cut(s) 21, 29, 95, 150, 211, 228, 238
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 119
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MfeI CAATTG 1 cut(s) 93
MluCI AATT 2 cut(s) 27, 93
MlyI GAGTC 1 cut(s) 247
MnlI CCTC 3 cut(s) 15, 107, 204
MspR9I CCNGG 2 cut(s) 9, 226
MunI CAATTG 1 cut(s) 93
MvaI CCWGG 2 cut(s) 9, 226
MwoI GCNNNNNNNGC 1 cut(s) 98
NdeI CATATG 1 cut(s) 249
NdeII GATC 1 cut(s) 219
NlaIII CATG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 12
PfoI TCCNGGA 1 cut(s) 7
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 7, 224
PspGI CCWGG 2 cut(s) 7, 224
Sau3AI GATC 1 cut(s) 219
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 9, 226
SetI ASST 4 cut(s) 126, 139, 215, 227
Sse9I AATT 2 cut(s) 27, 93
StyD4I CCNGG 2 cut(s) 7, 224
TaaI ACNGT 1 cut(s) 40
TaiI ACGT 1 cut(s) 126
TasI AATT 2 cut(s) 27, 93
TfiI GAWTC 1 cut(s) 12
TseFI GTSAC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.