Rh5CG468400

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0016617
Physical Location & Seq
Forward (+)
65312119 .. 65313647
1529 bp
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UTR
Exon/CDS
Intron
Rh5CG468400.1

Sequence Viewer

Length: 339 bp
ATGTACAAATCCAAATCAGGTGGATCTTTACTCGGAGTAGAGCATAAACCTAAAAAAATAGAAGAAGCCCAGTCAGGAACAAGAAAATTACATCGCGATTTAGATTGTATCTCGATGAAACAATACATCAATGAGAATTCTTTTTACGAGGCACAGACAGGAGAATTTGTCCTGGAAGCGGAAGAGCTGCTGAAGCTGCGCGAAACCATCACAAGGGTTTATGCACAAAGGACAGGCAAACCCCTATGGGTTGTATCCGAAGACATGGAAAGAGATGTTTTTATGTCAGCAACAGAAGCCCAAGCTCATGGAATTGTTGATCTTGTGGCGACTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04112 ko04212 map04112 map04212
Pfam Domains
Protein Families
CLP_protease

Protein Analysis

112

Amino Acids

12.74

Weight (kDa)

5.43

Isoelectric Point (pI)

34.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 49 - 111 2.1e-14 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 96, 201
AciI CCGC 1 cut(s) 179
AclWI GGATC 1 cut(s) 31
AcsI RAATTY 2 cut(s) 136, 164
AcuI CTGAAG 1 cut(s) 212
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 178, 213
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 3 cut(s) 187, 196, 305
AluI AGCT 3 cut(s) 187, 196, 305
AlwI GGATC 1 cut(s) 31
ApeKI GCWGC 2 cut(s) 187, 196
ApoI RAATTY 2 cut(s) 136, 164
AspLEI GCGC 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 174, 183
BccI CCATC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 173
BciVI GTATCC 1 cut(s) 265
BfuI GTATCC 1 cut(s) 265
BisI GCNGC 2 cut(s) 188, 197
BlsI GCNGC 2 cut(s) 189, 198
Bme1390I CCNGG 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 64
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 267
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 213
Bse1I ACTGG 1 cut(s) 70
BseBI CCWGG 1 cut(s) 173
BseLI CCNNNNNNNGG 2 cut(s) 178, 213
BseNI ACTGG 1 cut(s) 70
BseXI GCAGC 2 cut(s) 174, 183
Bsh1236I CGCG 2 cut(s) 96, 201
BslI CCNNNNNNNGG 2 cut(s) 178, 213
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 23, 319
Bsp68I TCGCGA 1 cut(s) 96
BspACI CCGC 1 cut(s) 179
BspFNI CGCG 2 cut(s) 96, 201
BspPI GGATC 1 cut(s) 31
BspQI GCTCTTC 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 70
BssMI GATC 2 cut(s) 23, 319
Bst2UI CCWGG 1 cut(s) 173
Bst6I CTCTTC 1 cut(s) 177
BstAUI TGTACA 1 cut(s) 3
BstFNI CGCG 2 cut(s) 96, 201
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 2 cut(s) 26, 322
BstMBI GATC 2 cut(s) 23, 319
BstMWI GCNNNNNNNGC 3 cut(s) 193, 196, 296
BstNI CCWGG 1 cut(s) 173
BstSCI CCNGG 1 cut(s) 171
BstUI CGCG 2 cut(s) 96, 201
BstV1I GCAGC 2 cut(s) 174, 183
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 23
BstXI CCANNNNNNTGG 1 cut(s) 308
BstYI RGATCY 1 cut(s) 23
BsuI GTATCC 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 77
BtuMI TCGCGA 1 cut(s) 96
CfoI GCGC 1 cut(s) 201
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 1 cut(s) 36
CviAII CATG 2 cut(s) 265, 308
CviJI RGCY 5 cut(s) 68, 187, 196, 299, 305
CviKI_1 RGCY 5 cut(s) 68, 187, 196, 299, 305
CviQI GTAC 1 cut(s) 4
DpnI GATC 2 cut(s) 25, 321
DpnII GATC 2 cut(s) 23, 319
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 212
EcoRI GAATTC 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 171
FaeI CATG 2 cut(s) 268, 311
FaiI YATR 6 cut(s) 45, 222, 247, 266, 284, 309
FatI CATG 2 cut(s) 264, 307
Fnu4HI GCNGC 2 cut(s) 188, 197
Fsp4HI GCNGC 2 cut(s) 188, 197
GlaI GCGC 1 cut(s) 200
GluI GCNGC 2 cut(s) 188, 197
HhaI GCGC 1 cut(s) 201
Hin1II CATG 2 cut(s) 268, 311
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
Hpy188I TCNGA 2 cut(s) 35, 259
Hpy188III TCNNGA 3 cut(s) 75, 95, 112
HpyCH4V TGCA 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 196, 296
Hsp92II CATG 2 cut(s) 268, 311
HspAI GCGC 1 cut(s) 199
Kzo9I GATC 2 cut(s) 23, 319
LguI GCTCTTC 1 cut(s) 177
LpnPI CCDG 7 cut(s) 3, 60, 83, 144, 158, 185, 219
Lsp1109I GCAGC 2 cut(s) 174, 183
MalI GATC 2 cut(s) 25, 321
MboI GATC 2 cut(s) 23, 319
MboII GAAGA 3 cut(s) 74, 194, 272
MflI RGATCY 1 cut(s) 23
MluCI AATT 4 cut(s) 86, 136, 164, 312
MnlI CCTC 1 cut(s) 142
MspR9I CCNGG 1 cut(s) 173
MvaI CCWGG 1 cut(s) 173
MvnI CGCG 2 cut(s) 96, 201
MwoI GCNNNNNNNGC 3 cut(s) 193, 196, 296
NdeII GATC 2 cut(s) 23, 319
NlaIII CATG 2 cut(s) 268, 311
NruI TCGCGA 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 177
PfoI TCCNGGA 1 cut(s) 171
PkrI GCNGC 2 cut(s) 189, 198
Psp6I CCWGG 1 cut(s) 171
PspGI CCWGG 1 cut(s) 171
PsuI RGATCY 1 cut(s) 23
RruI TCGCGA 1 cut(s) 96
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 177
SatI GCNGC 2 cut(s) 188, 197
Sau3AI GATC 2 cut(s) 23, 319
ScrFI CCNGG 1 cut(s) 173
SetI ASST 5 cut(s) 22, 52, 189, 198, 307
Sse9I AATT 4 cut(s) 86, 136, 164, 312
SsiI CCGC 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 171
TaqI TCGA 1 cut(s) 113
TasI AATT 4 cut(s) 86, 136, 164, 312
TatI WGTACW 1 cut(s) 3
TseI GCWGC 2 cut(s) 187, 196
TspDTI ATGAA 1 cut(s) 131
XapI RAATTY 2 cut(s) 136, 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.