Rh5DG283700

Bromodomain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007816
Physical Location & Seq
Reverse (-)
34907695 .. 34913869
6175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG283700.1

Sequence Viewer

Length: 2154 bp
ATGTCAACATCTCATTGTGGCAGGAAAGATACTTATGGCGTGTATGCAGAACCAGTCGATCCCGAAGAGCTTCCTGACTATCATGATGTCATTGAGCATCCCATGGATTTCGCGACGGTGAGGAAGCAGTTGGCAAATGGAGCCTATTCTACACTGGAACAATTTGAGAGAGATGTCTTTTTAATATGTTCAAACGCAATGCAATACAATTCACCTGAGACAATATACCATAAACAGGCATCTTCTATACAAGAGCTAGGAAGGAGAAAATTTGAGAGGTTAAGAATTGACTATGAACGGTCAGAAAAGGAGGTCAAATCAGTGCAGAAAACAAAGTCAAATTCCTTGGTAAAGAAACAGATAAAGAAGCCTCTGTCCCGAACATTACAGGAACCCATTGGCTCTGATTTCTCCTCAGGTGCCACTCTTGCCACTGCTGCTGAAGTCCAGAACAGTTCCCATCCAACACAAGGGGCTGGTTGTGAGAGAGCTAGCAACATTGATGGGCCTGTAGAGGGTAATTCATCCTTGAATGAAGCTAATCTAGAGAAGGCAGAAGAAATGCTATCAGGGAAGAGTCTACCATCTAAAGTTGGGAAGAAGCCATCTGTGTTTGATGACAACCGTCGTGCAACCTATAACATATCCAATCAGCCTGTGATCACATCAGAATCAATATTCACAACCTTTGAGGGTGAAATCAAGCAGTTTGTTGCAGTCGGGCTTCATGCAGAATATTCATATGCCAGGAGCCTGGCTCGATTCTCTGGAAGTCTTGGACCAGTTGCTTGGAAAGTTGCATCAAAGAGGATCGAACAGGCCTTACCAGATGGATGTAAATTTGGTCGTGGATGGGTCGAAGAATATGAGCCACTTCCAACCCCTGTACTGATGGTAGACAAAGGTACCCAAAATCAATCTGCTTTGGCTGCAAAATTCTTTTCACACAATGAATCAAGAAAGGATGATAGAACTTTAAGGACTTCAGTTCCTGCTAAGGATCGCCCTGTTACTAGGTCTGTTATAGAAGAGAGACAGCACTGTGTTAGTGTTCCTACTTCAGCGGGGAAACCATCATTTTTTGGTTCTTCTAGGGGACATTACTCGGAAGAGAAACCGCCTGTTATTAGTTCTGTCGGAGGAAAAGGTGGTAATGCAGTTAGTGCCATTCATAAGCAGCAAAATCCACAATCCAGGTTTATTGAGAGTGGGAAACAGGTTCCGAAGAAAGTTGAGTTGAACTCTGTACCCTCAGTTAATCAAAATAATGCCAACCTTGTTGCTGAAAAGCAATCACCAAGAAACTCAGAGCCAGCAACTTCAAGGTCCAGAGGTACATCATCGAGGAACATGAATCTTCAGCAGCCTGTACCTTTTAAGATGCCGGATTCTAATGGTGTTATTACCTCACGGTTGCCTAATGGGAAAGGTGCGAGTGCTTGTATGGAGAATCGGATGATTGGTTCTTCTGATAGGGCTCCTAGCCAAATGGAAAGAACTGAAGCTTATTTTCCTCATGCACAGGAGCAGGGTCTTAGTGACCCAGTACAGTTGATGAAAAAATTGGCGGAGAAGACTCAAAAGCAACAGCAGTCATCAATTCAATCGTCAATTGATACTAAACCTGTCGTGTCATCGGTTCCATCAACGAGAAGAGATGATCCAAGCAATGCTGCAGCAGCTGCTGCCCGTGCTTGGATGTCTATAGGGGGTGCATCCTTTAAACAGCCAACAGACAATCCGACAGTGCCCAAAAGTCAGATCTATGCAGATTCATTATATAACCCATCACGAGAGATTAATTCACAAATTTCACGAGTTCGGGGGGAGGTTCCTAACTCTGGTGCAATGCAATTTCAGACTGAGAACAATTTTTCATTTCCAACTTTTTTACCACGACCTGTTCATATGGTGAATGAACCACAGTTCCAAAATCGACCTATATTTTTCCCTCAGTTGGCAGCAGCTGATTTGTCTAGGTTTCAGGTGCCGCCCCCTTGGAGGGGTCTCAGTCCTTGTGCCCAGCCAAGGCAGAAACAAGAATGTCTTCCTCCAGATTTAAATATTGGTTTCCAGTCCCCAGGGTCTCCAGTGAAACAGTCATCTGGTGTTATGGTTGACTCTCAGCAGCCAGACCTGGCTTTGCAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0042393 GO:0070577 GO:0140030 GO:0140033
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromodomain

Protein Analysis

717

Amino Acids

78.87

Weight (kDa)

9.09

Isoelectric Point (pI)

64.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromodomain PF00439 9 - 81 1.3e-22 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 905
AccB1I GGYRCC 3 cut(s) 419, 905, 1987
AccI GTMKAC 2 cut(s) 580, 897
AccII CGCG 1 cut(s) 113
AciI CCGC 4 cut(s) 1064, 1118, 1568, 1991
AclWI GGATC 4 cut(s) 53, 818, 1008, 1655
AcsI RAATTY 5 cut(s) 269, 340, 839, 935, 1809
AcuI CTGAAG 5 cut(s) 462, 969, 1044, 1343, 1521
AfaI GTAC 6 cut(s) 888, 907, 1248, 1336, 1371, 1548
AgsI TTSAA 5 cut(s) 192, 532, 1240, 1323, 1604
AjnI CCWGG 5 cut(s) 746, 753, 1193, 2080, 2136
AloI GAACNNNNNNTCC 4 cut(s) 1447, 1479, 1911, 1943
AluBI AGCT 7 cut(s) 70, 256, 491, 539, 1505, 1682, 1967
AluI AGCT 7 cut(s) 70, 256, 491, 539, 1505, 1682, 1967
Alw26I GTCTC 4 cut(s) 212, 1027, 2012, 2091
AlwI GGATC 4 cut(s) 53, 818, 1008, 1655
AlwNI CAGNNNCTG 4 cut(s) 992, 1682, 1685, 1967
AoxI GGCC 2 cut(s) 506, 819
ApoI RAATTY 5 cut(s) 269, 340, 839, 935, 1809
AseI ATTAAT 1 cut(s) 1800
Asp700I GAANNNNTTC 2 cut(s) 69, 2046
Asp718I GGTACC 1 cut(s) 905
AspS9I GGNCC 3 cut(s) 506, 779, 1326
AsuHPI GGTGA 5 cut(s) 130, 204, 707, 1287, 1924
AsuNHI GCTAGC 1 cut(s) 491
AvaII GGWCC 2 cut(s) 779, 1326
AxyI CCTNAGG 1 cut(s) 415
BaeGI GKGCMC 2 cut(s) 1752, 2023
BaeI ACNNNNGTAYC 2 cut(s) 889, 922
BanI GGYRCC 3 cut(s) 419, 905, 1987
BanII GRGCYC 1 cut(s) 1480
BauI CACGAG 2 cut(s) 1791, 1815
BbsI GAAGAC 2 cut(s) 1580, 2039
BciT130I CCWGG 5 cut(s) 748, 755, 1195, 2082, 2138
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 4 cut(s) 212, 1027, 2012, 2091
BfaI CTAG 7 cut(s) 257, 492, 545, 1014, 1092, 1482, 1977
BfmI CTRYAG 3 cut(s) 510, 1674, 1704
BglII AGATCT 1 cut(s) 1761
Bme1390I CCNGG 5 cut(s) 748, 755, 1195, 2082, 2138
Bme18I GGWCC 2 cut(s) 779, 1326
BmgT120I GGNCC 3 cut(s) 506, 779, 1326
BmrFI CCNGG 5 cut(s) 748, 755, 1195, 2082, 2138
BmrI ACTGGG 1 cut(s) 1538
BmsI GCATC 5 cut(s) 106, 248, 809, 1371, 1724
BmtI GCTAGC 1 cut(s) 495
BmuI ACTGGG 1 cut(s) 1538
BoxI GACNNNNGTC 1 cut(s) 624
BpiI GAAGAC 2 cut(s) 1580, 2039
BplI GAGNNNNNCTC 4 cut(s) 742, 774, 1226, 1258
BpmI CTGGAG 2 cut(s) 2037, 2073
Bpu10I CCTNAGC 1 cut(s) 996
BsaI GGTCTC 2 cut(s) 2012, 2091
BsaJI CCNNGG 6 cut(s) 102, 345, 1997, 2027, 2080, 2081
Bse1I ACTGG 6 cut(s) 53, 159, 782, 1544, 2074, 2090
Bse21I CCTNAGG 1 cut(s) 415
Bse3DI GCAATG 3 cut(s) 204, 1675, 1854
BseBI CCWGG 5 cut(s) 748, 755, 1195, 2082, 2138
BseDI CCNNGG 6 cut(s) 102, 345, 1997, 2027, 2080, 2081
BseGI GGATG 9 cut(s) 97, 460, 524, 839, 857, 970, 1461, 1704, 1715
BseMI GCAATG 3 cut(s) 204, 1675, 1854
BseMII CTCAG 8 cut(s) 207, 429, 1266, 1320, 1854, 1967, 2023, 2138
BseNI ACTGG 6 cut(s) 53, 159, 782, 1544, 2074, 2090
BseRI GAGGAG 1 cut(s) 403
BseSI GKGCMC 2 cut(s) 1752, 2023
BseYI CCCAGC 1 cut(s) 2022
BsgI GTGCAG 1 cut(s) 344
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 2 cut(s) 508, 821
BshNI GGYRCC 3 cut(s) 419, 905, 1987
BsiSI CCGG 1 cut(s) 1385
BslFI GGGAC 3 cut(s) 361, 1110, 2062
BsmAI GTCTC 4 cut(s) 212, 1027, 2012, 2091
BsmFI GGGAC 3 cut(s) 361, 1110, 2062
BsnI GGCC 2 cut(s) 508, 821
Bso31I GGTCTC 2 cut(s) 2012, 2091
Bsp1286I GDGCHC 3 cut(s) 1480, 1752, 2023
Bsp143I GATC 6 cut(s) 58, 660, 810, 1000, 1660, 1761
Bsp19I CCATGG 1 cut(s) 102
Bsp68I TCGCGA 1 cut(s) 113
BspACI CCGC 4 cut(s) 1064, 1118, 1568, 1991
BspANI GGCC 2 cut(s) 508, 821
BspCNI CTCAG 8 cut(s) 208, 428, 1265, 1319, 1855, 1966, 2022, 2137
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 1 cut(s) 82
BspMAI CTGCAG 1 cut(s) 1678
BspOI GCTAGC 1 cut(s) 495
BspPI GGATC 4 cut(s) 53, 818, 1008, 1655
BspQI GCTCTTC 1 cut(s) 60
BspT107I GGYRCC 3 cut(s) 419, 905, 1987
BspTNI GGTCTC 2 cut(s) 2012, 2091
BsrDI GCAATG 3 cut(s) 204, 1675, 1854
BsrI ACTGG 6 cut(s) 53, 159, 782, 1544, 2074, 2090
BssECI CCNNGG 6 cut(s) 102, 345, 1997, 2027, 2080, 2081
BssMI GATC 6 cut(s) 58, 660, 810, 1000, 1660, 1761
BssSI CACGAG 2 cut(s) 1791, 1815
BssT1I CCWWGG 4 cut(s) 102, 345, 1997, 2027
Bst2BI CACGAG 2 cut(s) 1791, 1815
Bst2UI CCWGG 5 cut(s) 748, 755, 1195, 2082, 2138
Bst6I CTCTTC 5 cut(s) 60, 569, 1023, 1104, 1648
BstAPI GCANNNNNTGC 3 cut(s) 1163, 1682, 1685
BstC8I GCNNGC 2 cut(s) 493, 1314
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 9 cut(s) 97, 460, 524, 839, 857, 970, 1461, 1704, 1715
BstFNI CGCG 1 cut(s) 113
BstKTI GATC 6 cut(s) 61, 663, 813, 1003, 1663, 1764
BstMAI GTCTC 4 cut(s) 212, 1027, 2012, 2091
BstMBI GATC 6 cut(s) 58, 660, 810, 1000, 1660, 1761
BstMWI GCNNNNNNNGC 9 cut(s) 140, 428, 437, 929, 1163, 1679, 1682, 1685, 1691
BstNI CCWGG 5 cut(s) 748, 755, 1195, 2082, 2138
BstPAI GACNNNNGTC 1 cut(s) 624
BstSCI CCNGG 5 cut(s) 746, 753, 1193, 2080, 2136
BstSFI CTRYAG 3 cut(s) 510, 1674, 1704
BstSLI GKGCMC 2 cut(s) 1752, 2023
BstUI CGCG 1 cut(s) 113
BstV2I GAAGAC 2 cut(s) 1580, 2039
BstX2I RGATCY 1 cut(s) 1761
BstXI CCANNNNNNTGG 2 cut(s) 754, 789
BstYI RGATCY 1 cut(s) 1761
Bsu36I CCTNAGG 1 cut(s) 415
BsuRI GGCC 2 cut(s) 508, 821
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 9 cut(s) 97, 460, 524, 839, 857, 970, 1461, 1704, 1715
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 6 cut(s) 152, 327, 432, 1039, 1752, 2097
BtuMI TCGCGA 1 cut(s) 113
Cac8I GCNNGC 2 cut(s) 493, 1314
CaiI CAGNNNCTG 4 cut(s) 992, 1682, 1685, 1967
CciI TCATGA 1 cut(s) 82
Cfr13I GGNCC 3 cut(s) 506, 779, 1326
Csp6I GTAC 6 cut(s) 887, 906, 1247, 1335, 1370, 1547
CviAII CATG 5 cut(s) 83, 103, 728, 1351, 1517
CviQI GTAC 6 cut(s) 887, 906, 1247, 1335, 1370, 1547
DpnI GATC 6 cut(s) 60, 662, 812, 1002, 1662, 1763
DpnII GATC 6 cut(s) 58, 660, 810, 1000, 1660, 1761
DraI TTTAAA 2 cut(s) 1723, 2061
Eam1104I CTCTTC 5 cut(s) 60, 569, 1023, 1104, 1648
EarI CTCTTC 5 cut(s) 60, 569, 1023, 1104, 1648
EciI GGCGGA 1 cut(s) 1583
Eco130I CCWWGG 4 cut(s) 102, 345, 1997, 2027
Eco147I AGGCCT 1 cut(s) 821
Eco24I GRGCYC 1 cut(s) 1480
Eco31I GGTCTC 2 cut(s) 2012, 2091
Eco47I GGWCC 2 cut(s) 779, 1326
Eco57I CTGAAG 5 cut(s) 462, 969, 1044, 1343, 1521
Eco81I CCTNAGG 1 cut(s) 415
EcoRII CCWGG 5 cut(s) 746, 753, 1193, 2080, 2136
EcoT14I CCWWGG 4 cut(s) 102, 345, 1997, 2027
EcoT38I GRGCYC 1 cut(s) 1480
ErhI CCWWGG 4 cut(s) 102, 345, 1997, 2027
FaeI CATG 5 cut(s) 86, 106, 731, 1354, 1520
FalI AAGNNNNNCTT 2 cut(s) 2031, 2063
FaqI GGGAC 3 cut(s) 361, 1110, 2062
FatI CATG 5 cut(s) 82, 102, 727, 1350, 1516
FauI CCCGC 1 cut(s) 1057
FauNDI CATATG 2 cut(s) 742, 1908
FbaI TGATCA 1 cut(s) 660
FblI GTMKAC 2 cut(s) 580, 897
FokI GGATG 9 cut(s) 84, 447, 511, 846, 864, 977, 1468, 1702, 1711
FriOI GRGCYC 1 cut(s) 1480
FspBI CTAG 7 cut(s) 257, 492, 545, 1014, 1092, 1482, 1977
GsaI CCCAGC 1 cut(s) 2026
GsuI CTGGAG 2 cut(s) 2037, 2073
HaeIII GGCC 2 cut(s) 508, 821
HapII CCGG 1 cut(s) 1385
Hin1II CATG 5 cut(s) 86, 106, 731, 1354, 1520
HincII GTYRAC 2 cut(s) 6, 2119
HindII GTYRAC 2 cut(s) 6, 2119
HindIII AAGCTT 1 cut(s) 1503
HpaII CCGG 1 cut(s) 1385
HphI GGTGA 5 cut(s) 130, 204, 707, 1287, 1924
Hpy166II GTNNAC 4 cut(s) 6, 581, 898, 2119
Hpy8I GTNNAC 4 cut(s) 6, 581, 898, 2119
Hpy99I CGWCG 2 cut(s) 118, 630
HpyAV CCTTC 2 cut(s) 255, 544
HpyF10VI GCNNNNNNNGC 9 cut(s) 140, 428, 437, 929, 1163, 1679, 1682, 1685, 1691
Hsp92II CATG 5 cut(s) 86, 106, 731, 1354, 1520
KpnI GGTACC 1 cut(s) 909
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 6 cut(s) 58, 660, 810, 1000, 1660, 1761
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 4 cut(s) 140, 750, 1483, 1525
LweI GCATC 5 cut(s) 106, 248, 809, 1371, 1724
MaeI CTAG 7 cut(s) 257, 492, 545, 1014, 1092, 1482, 1977
MaeIII GTNAC 2 cut(s) 1009, 1538
MalI GATC 6 cut(s) 60, 662, 812, 1002, 1662, 1763
MboI GATC 6 cut(s) 58, 660, 810, 1000, 1660, 1761
MfeI CAATTG 1 cut(s) 1611
MflI RGATCY 1 cut(s) 1761
MhlI GDGCHC 3 cut(s) 1480, 1752, 2023
MlyI GAGTC 3 cut(s) 586, 1570, 2114
MmeI TCCRAC 5 cut(s) 488, 902, 1117, 1766, 1907
MroXI GAANNNNTTC 2 cut(s) 69, 2046
MseI TTAA 8 cut(s) 182, 281, 977, 1257, 1377, 1722, 1800, 2060
MspA1I CMGCKG 3 cut(s) 1064, 1682, 1967
MspI CCGG 1 cut(s) 1385
MspR9I CCNGG 5 cut(s) 748, 755, 1195, 2082, 2138
MunI CAATTG 1 cut(s) 1611
MvaI CCWGG 5 cut(s) 748, 755, 1195, 2082, 2138
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 9 cut(s) 140, 428, 437, 929, 1163, 1679, 1682, 1685, 1691
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 2 cut(s) 742, 1908
NdeII GATC 6 cut(s) 58, 660, 810, 1000, 1660, 1761
NheI GCTAGC 1 cut(s) 491
NlaIII CATG 5 cut(s) 86, 106, 731, 1354, 1520
NmuCI GTSAC 1 cut(s) 1538
NruI TCGCGA 1 cut(s) 113
PagI TCATGA 1 cut(s) 82
PasI CCCWGGG 1 cut(s) 2081
PceI AGGCCT 1 cut(s) 821
PciSI GCTCTTC 1 cut(s) 60
PdmI GAANNNNTTC 2 cut(s) 69, 2046
PfeI GAWTC 7 cut(s) 671, 762, 953, 1354, 1388, 1450, 1772
PleI GAGTC 3 cut(s) 585, 1570, 2114
PpsI GAGTC 3 cut(s) 585, 1570, 2114
PshAI GACNNNNGTC 1 cut(s) 624
PshBI ATTAAT 1 cut(s) 1800
Psp6I CCWGG 5 cut(s) 746, 753, 1193, 2080, 2136
PspFI CCCAGC 1 cut(s) 2022
PspGI CCWGG 5 cut(s) 746, 753, 1193, 2080, 2136
PspPI GGNCC 3 cut(s) 506, 779, 1326
PsrI GAACNNNNNNTAC 2 cut(s) 807, 839
PstI CTGCAG 1 cut(s) 1678
PstNI CAGNNNCTG 4 cut(s) 992, 1682, 1685, 1967
PsuI RGATCY 1 cut(s) 1761
PvuII CAGCTG 2 cut(s) 1682, 1967
RruI TCGCGA 1 cut(s) 113
RsaI GTAC 6 cut(s) 888, 907, 1248, 1336, 1371, 1548
RsaNI GTAC 6 cut(s) 887, 906, 1247, 1335, 1370, 1547
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 8 cut(s) 182, 281, 977, 1257, 1377, 1722, 1800, 2060
Sau3AI GATC 6 cut(s) 58, 660, 810, 1000, 1660, 1761
Sau96I GGNCC 3 cut(s) 506, 779, 1326
SchI GAGTC 3 cut(s) 586, 1570, 2114
ScrFI CCNGG 5 cut(s) 748, 755, 1195, 2082, 2138
SduI GDGCHC 3 cut(s) 1480, 1752, 2023
SfaNI GCATC 5 cut(s) 106, 248, 809, 1371, 1724
SfcI CTRYAG 3 cut(s) 510, 1674, 1704
SinI GGWCC 2 cut(s) 779, 1326
SmiI ATTTAAAT 1 cut(s) 2061
SseBI AGGCCT 1 cut(s) 821
SsiI CCGC 4 cut(s) 1064, 1118, 1568, 1991
SspI AATATT 3 cut(s) 678, 737, 2065
SspMI CTAG 7 cut(s) 257, 492, 545, 1014, 1092, 1482, 1977
StuI AGGCCT 1 cut(s) 821
StyD4I CCNGG 5 cut(s) 746, 753, 1193, 2080, 2136
StyI CCWWGG 4 cut(s) 102, 345, 1997, 2027
SwaI ATTTAAAT 1 cut(s) 2061
TaqI TCGA 6 cut(s) 57, 760, 813, 858, 1343, 1936
TatI WGTACW 2 cut(s) 886, 1546
TauI GCSGC 1 cut(s) 1993
TfiI GAWTC 7 cut(s) 671, 762, 953, 1354, 1388, 1450, 1772
Tru1I TTAA 8 cut(s) 182, 281, 977, 1257, 1377, 1722, 1800, 2060
Tru9I TTAA 8 cut(s) 182, 281, 977, 1257, 1377, 1722, 1800, 2060
TscAI CASTG 6 cut(s) 159, 327, 439, 1046, 1752, 2097
TseFI GTSAC 1 cut(s) 1538
Tsp45I GTSAC 1 cut(s) 1538
TspRI CASTG 6 cut(s) 159, 327, 439, 1046, 1752, 2097
VpaK11BI GGWCC 2 cut(s) 779, 1326
VspI ATTAAT 1 cut(s) 1800
XapI RAATTY 5 cut(s) 269, 340, 839, 935, 1809
XbaI TCTAGA 1 cut(s) 544
XmiI GTMKAC 2 cut(s) 580, 897
XmnI GAANNNNTTC 2 cut(s) 69, 2046
XspI CTAG 7 cut(s) 257, 492, 545, 1014, 1092, 1482, 1977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.