Rh6BG179700

atnic1,nic1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003846
Physical Location & Seq
Forward (+)
30911893 .. 30915177
3285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG179700.1

Sequence Viewer

Length: 675 bp
ATGGTGTCTCAGACAATTGATCTTTTGAAGAATGAAATTCCTCTGGACCAGGAACCTGTGGATCTGTCAGAGGATGTTGTCACTGGACTTGTTCTAGTGGACATAATCAATGGCTTCTGTACTGTTGGTGCTGGAAATCTGGCTCCAAGAGAACCCGATAGGCAGATCTCGGAGATGATTAGTGAGTCTGCAAGACTTGCCAGACACTTTTGTGAAAAGAAATGGCCAGTTTTGGCATTCATTGATTCTCATCAACCTGAAAAGCTGGAAGACCCTTATCCCCTTCATTGTATCGAGGGCACTGATGAATCAAACTTGGTCCCTGCATTAAGTTGGTTGGAGAAAGAACCAAATGTCACAATCAGGCGCAAAGATTGCTACGATGGATTCGTAGGCTCAATTCAACCTGATGGTTCAAATGCTTTTGTTGATTGGGTGAAGAAAAATCAGATCAGAACTTTATTAGTGGTAGGAATATGCACGGACATCTGTGTTCTGGACTTTGTTTGTTCCACAATCTCAGCTAGAAACCGTGGTTTTCTGACCCCTTTGGTGGACGTAGTGGTTTACTCGCGTGCCTGCGCCACATTTAATGTTCCTCTTCAGGTTGCCAGAAACACCAAAGGAGCTTTAGCTCACCCTCAGGTAATCATTTGCCAACACATCCTAGAATAA

Protein Analysis

224

Amino Acids

24.88

Weight (kDa)

4.96

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 29 - 203 5e-24 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 574
AclWI GGATC 1 cut(s) 69
AcoI YGGCCR 1 cut(s) 224
AcsI RAATTY 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 587
AfaI GTAC 1 cut(s) 121
AfiI CCNNNNNNNGG 1 cut(s) 553
AgsI TTSAA 3 cut(s) 28, 404, 417
AjnI CCWGG 1 cut(s) 48
AleI CACNNNNGTG 1 cut(s) 210
AluBI AGCT 4 cut(s) 265, 524, 629, 635
AluI AGCT 4 cut(s) 265, 524, 629, 635
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 1 cut(s) 69
AoxI GGCC 1 cut(s) 224
ApoI RAATTY 1 cut(s) 36
AspLEI GCGC 2 cut(s) 369, 584
AspS9I GGNCC 2 cut(s) 46, 319
AsuHPI GGTGA 2 cut(s) 448, 629
AvaII GGWCC 2 cut(s) 46, 319
AxyI CCTNAGG 1 cut(s) 642
BaeGI GKGCMC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 226
BbsI GAAGAC 1 cut(s) 276
BccI CCATC 2 cut(s) 377, 404
BciT130I CCWGG 1 cut(s) 50
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 3 cut(s) 95, 525, 668
BglII AGATCT 1 cut(s) 165
Bme1390I CCNGG 1 cut(s) 50
Bme18I GGWCC 2 cut(s) 46, 319
BmgT120I GGNCC 2 cut(s) 46, 319
BmiI GGNNCC 3 cut(s) 54, 144, 321
BmrFI CCNGG 1 cut(s) 50
BpiI GAAGAC 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 532
Bsc4I CCNNNNNNNGG 1 cut(s) 553
Bse1I ACTGG 2 cut(s) 88, 227
Bse21I CCTNAGG 1 cut(s) 642
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 532
BseGI GGATG 2 cut(s) 79, 663
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 1 cut(s) 553
BseMII CTCAG 3 cut(s) 23, 534, 656
BseNI ACTGG 2 cut(s) 88, 227
BseSI GKGCMC 1 cut(s) 302
Bsh1236I CGCG 1 cut(s) 574
BshFI GGCC 1 cut(s) 226
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 553
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 236
BsnI GGCC 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 302
Bsp143I GATC 4 cut(s) 19, 61, 165, 450
BspANI GGCC 1 cut(s) 226
BspCNI CTCAG 3 cut(s) 22, 533, 655
BspFNI CGCG 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 54, 144, 321
BspPI GGATC 1 cut(s) 69
BsrI ACTGG 2 cut(s) 88, 227
BssECI CCNNGG 1 cut(s) 532
BssMI GATC 4 cut(s) 19, 61, 165, 450
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 124, 533
Bst6I CTCTTC 1 cut(s) 606
BstAPI GCANNNNNTGC 2 cut(s) 197, 375
BstC8I GCNNGC 2 cut(s) 576, 580
BstDEI CTNAG 3 cut(s) 9, 520, 642
BstDSI CCRYGG 1 cut(s) 532
BstF5I GGATG 2 cut(s) 79, 663
BstFNI CGCG 1 cut(s) 574
BstHHI GCGC 2 cut(s) 369, 584
BstKTI GATC 4 cut(s) 22, 64, 168, 453
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 4 cut(s) 19, 61, 165, 450
BstMWI GCNNNNNNNGC 2 cut(s) 197, 375
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 48
BstSLI GKGCMC 1 cut(s) 302
BstUI CGCG 1 cut(s) 574
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 2 cut(s) 61, 165
BstYI RGATCY 2 cut(s) 61, 165
Bsu36I CCTNAGG 1 cut(s) 642
BsuRI GGCC 1 cut(s) 226
BtgI CCRYGG 1 cut(s) 532
BtsCI GGATG 2 cut(s) 79, 663
BtsIMutI CAGTG 2 cut(s) 81, 300
Cac8I GCNNGC 2 cut(s) 576, 580
CfoI GCGC 2 cut(s) 369, 584
Cfr13I GGNCC 2 cut(s) 46, 319
Csp6I GTAC 1 cut(s) 120
CviJI RGCY 8 cut(s) 114, 143, 226, 265, 396, 524, 629, 635
CviKI_1 RGCY 8 cut(s) 114, 143, 226, 265, 396, 524, 629, 635
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 3 cut(s) 9, 520, 642
DpnI GATC 4 cut(s) 21, 63, 167, 452
DpnII GATC 4 cut(s) 19, 61, 165, 450
EaeI YGGCCR 1 cut(s) 224
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco47I GGWCC 2 cut(s) 46, 319
Eco57I CTGAAG 1 cut(s) 587
Eco81I CCTNAGG 1 cut(s) 642
EcoRII CCWGG 1 cut(s) 48
FaiI YATR 2 cut(s) 104, 478
FaqI GGGAC 1 cut(s) 305
FokI GGATG 2 cut(s) 86, 650
FspBI CTAG 3 cut(s) 95, 525, 668
GlaI GCGC 2 cut(s) 368, 583
HaeIII GGCC 1 cut(s) 226
HhaI GCGC 2 cut(s) 369, 584
Hin6I GCGC 2 cut(s) 367, 582
HinP1I GCGC 2 cut(s) 367, 582
HinfI GANTC 4 cut(s) 185, 245, 308, 387
HphI GGTGA 2 cut(s) 448, 629
Hpy166II GTNNAC 3 cut(s) 100, 556, 568
Hpy188I TCNGA 6 cut(s) 12, 70, 172, 450, 455, 543
Hpy188III TCNNGA 2 cut(s) 44, 497
Hpy8I GTNNAC 3 cut(s) 100, 556, 568
HpyAV CCTTC 1 cut(s) 293
HpyCH4III ACNGT 2 cut(s) 124, 533
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 3 cut(s) 191, 326, 480
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 375
HpyF3I CTNAG 3 cut(s) 9, 520, 642
HpySE526I ACGT 1 cut(s) 558
HspAI GCGC 2 cut(s) 367, 582
Kzo9I GATC 4 cut(s) 19, 61, 165, 450
LmnI GCTCC 2 cut(s) 148, 626
MaeI CTAG 3 cut(s) 95, 525, 668
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 2 cut(s) 79, 355
MalI GATC 4 cut(s) 21, 63, 167, 452
MboI GATC 4 cut(s) 19, 61, 165, 450
MboII GAAGA 4 cut(s) 40, 281, 451, 593
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 2 cut(s) 61, 165
MhlI GDGCHC 1 cut(s) 302
MlsI TGGCCA 1 cut(s) 226
MluCI AATT 3 cut(s) 15, 36, 399
MluNI TGGCCA 1 cut(s) 226
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 1 cut(s) 318
MnlI CCTC 5 cut(s) 51, 64, 289, 609, 651
Mox20I TGGCCA 1 cut(s) 226
MscI TGGCCA 1 cut(s) 226
MseI TTAA 2 cut(s) 329, 591
MslI CAYNNNNRTG 1 cut(s) 210
Msp20I TGGCCA 1 cut(s) 226
MspR9I CCNGG 1 cut(s) 50
MunI CAATTG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 236
MvaI CCWGG 1 cut(s) 50
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 2 cut(s) 197, 375
NdeII GATC 4 cut(s) 19, 61, 165, 450
NlaIV GGNNCC 3 cut(s) 54, 144, 321
NmuCI GTSAC 2 cut(s) 79, 355
OliI CACNNNNGTG 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PctI GAATGC 1 cut(s) 236
PfeI GAWTC 3 cut(s) 245, 308, 387
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 3 cut(s) 54, 144, 321
PspPI GGNCC 2 cut(s) 46, 319
PsuI RGATCY 2 cut(s) 61, 165
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
RseI CAYNNNNRTG 1 cut(s) 210
SaqAI TTAA 2 cut(s) 329, 591
Sau3AI GATC 4 cut(s) 19, 61, 165, 450
Sau96I GGNCC 2 cut(s) 46, 319
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 1 cut(s) 302
SinI GGWCC 2 cut(s) 46, 319
SmiMI CAYNNNNRTG 1 cut(s) 210
Sse9I AATT 3 cut(s) 15, 36, 399
SspMI CTAG 3 cut(s) 95, 525, 668
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 2 cut(s) 124, 533
TaiI ACGT 1 cut(s) 561
TaqI TCGA 1 cut(s) 294
TasI AATT 3 cut(s) 15, 36, 399
TatI WGTACW 1 cut(s) 119
TfiI GAWTC 3 cut(s) 245, 308, 387
Tru1I TTAA 2 cut(s) 329, 591
Tru9I TTAA 2 cut(s) 329, 591
TscAI CASTG 2 cut(s) 88, 307
TseFI GTSAC 2 cut(s) 79, 355
Tsp45I GTSAC 2 cut(s) 79, 355
TspDTI ATGAA 4 cut(s) 48, 229, 275, 321
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 2 cut(s) 88, 307
VpaK11BI GGWCC 2 cut(s) 46, 319
XapI RAATTY 1 cut(s) 36
XspI CTAG 3 cut(s) 95, 525, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.