Zinc finger CCCH domain-containing protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 745, 2713 |
| Acc36I | ACCTGC | 2 cut(s) | 2092, 2428 |
| AccB1I | GGYRCC | 5 cut(s) | 805, 829, 1505, 1683, 2189 |
| AccI | GTMKAC | 3 cut(s) | 67, 509, 1285 |
| AccII | CGCG | 1 cut(s) | 698 |
| AccIII | TCCGGA | 1 cut(s) | 160 |
| AciI | CCGC | 3 cut(s) | 1380, 1509, 1663 |
| AclWI | GGATC | 2 cut(s) | 523, 1334 |
| AcsI | RAATTY | 7 cut(s) | 108, 642, 942, 1124, 1190, 1706, 1999 |
| AcuI | CTGAAG | 7 cut(s) | 337, 802, 807, 855, 1506, 2586, 2598 |
| AfaI | GTAC | 5 cut(s) | 97, 156, 1417, 2185, 2815 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 2178, 2199 |
| AflIII | ACRYGT | 1 cut(s) | 129 |
| AgeI | ACCGGT | 1 cut(s) | 2269 |
| AjiI | CACGTC | 1 cut(s) | 130 |
| AjnI | CCWGG | 4 cut(s) | 1288, 1299, 1958, 2630 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 218, 250, 390, 422, 2117, 2149 |
| Alw21I | GWGCWC | 2 cut(s) | 168, 1164 |
| Alw26I | GTCTC | 4 cut(s) | 731, 1941, 2372, 2744 |
| AlwI | GGATC | 2 cut(s) | 523, 1334 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2381 |
| Ama87I | CYCGRG | 1 cut(s) | 2644 |
| Aor13HI | TCCGGA | 1 cut(s) | 160 |
| AoxI | GGCC | 1 cut(s) | 2169 |
| ApeKI | GCWGC | 5 cut(s) | 136, 272, 1913, 2435, 2581 |
| ApoI | RAATTY | 7 cut(s) | 108, 642, 942, 1124, 1190, 1706, 1999 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1778, 1810 |
| AseI | ATTAAT | 1 cut(s) | 414 |
| AsiGI | ACCGGT | 1 cut(s) | 2269 |
| AspLEI | GCGC | 2 cut(s) | 698, 761 |
| AspS9I | GGNCC | 3 cut(s) | 437, 1529, 2170 |
| AsuHPI | GGTGA | 7 cut(s) | 53, 650, 1706, 1871, 2310, 2345, 2537 |
| AsuII | TTCGAA | 2 cut(s) | 106, 396 |
| AsuNHI | GCTAGC | 1 cut(s) | 1916 |
| AvaI | CYCGRG | 1 cut(s) | 2644 |
| AvaII | GGWCC | 2 cut(s) | 437, 1529 |
| AxyI | CCTNAGG | 1 cut(s) | 441 |
| BanI | GGYRCC | 5 cut(s) | 805, 829, 1505, 1683, 2189 |
| BanII | GRGCYC | 2 cut(s) | 12, 168 |
| BbsI | GAAGAC | 2 cut(s) | 695, 741 |
| Bbv12I | GWGCWC | 2 cut(s) | 168, 1164 |
| BbvI | GCAGC | 5 cut(s) | 148, 284, 1900, 2422, 2568 |
| BccI | CCATC | 9 cut(s) | 302, 382, 448, 1010, 1046, 1051, 2230, 2318, 2358 |
| BciT130I | CCWGG | 4 cut(s) | 1290, 1301, 1960, 2632 |
| BciVI | GTATCC | 1 cut(s) | 1827 |
| BclI | TGATCA | 1 cut(s) | 1798 |
| BcoDI | GTCTC | 4 cut(s) | 731, 1941, 2372, 2744 |
| BfaI | CTAG | 8 cut(s) | 693, 888, 1242, 1349, 1604, 1917, 2145, 2463 |
| BfmI | CTRYAG | 4 cut(s) | 600, 1650, 2118, 2436 |
| BfuAI | ACCTGC | 2 cut(s) | 2092, 2428 |
| BfuI | GTATCC | 1 cut(s) | 1827 |
| BisI | GCNGC | 7 cut(s) | 137, 273, 1509, 1663, 1914, 2436, 2582 |
| BlsI | GCNGC | 7 cut(s) | 138, 274, 1510, 1664, 1915, 2437, 2583 |
| BmcAI | AGTACT | 1 cut(s) | 2185 |
| Bme1390I | CCNGG | 4 cut(s) | 1290, 1301, 1960, 2632 |
| Bme18I | GGWCC | 2 cut(s) | 437, 1529 |
| BmeT110I | CYCGRG | 1 cut(s) | 2644 |
| BmgBI | CACGTC | 1 cut(s) | 130 |
| BmgT120I | GGNCC | 3 cut(s) | 437, 1529, 2170 |
| BmrFI | CCNGG | 4 cut(s) | 1290, 1301, 1960, 2632 |
| BmsI | GCATC | 6 cut(s) | 865, 1439, 1489, 1860, 2199, 2636 |
| BmtI | GCTAGC | 1 cut(s) | 1920 |
| BpiI | GAAGAC | 2 cut(s) | 695, 741 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1239, 1271 |
| BpmI | CTGGAG | 2 cut(s) | 485, 1311 |
| Bpu14I | TTCGAA | 2 cut(s) | 106, 396 |
| BpuEI | CTTGAG | 2 cut(s) | 62, 2545 |
| BsaI | GGTCTC | 1 cut(s) | 2744 |
| BsaJI | CCNNGG | 3 cut(s) | 1300, 1893, 2645 |
| BsaWI | WCCGGW | 5 cut(s) | 160, 512, 610, 980, 2269 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2173, 2203 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 2178, 2199 |
| Bse118I | RCCGGY | 1 cut(s) | 2269 |
| Bse1I | ACTGG | 4 cut(s) | 468, 1765, 2191, 2331 |
| Bse21I | CCTNAGG | 1 cut(s) | 441 |
| Bse3DI | GCAATG | 3 cut(s) | 738, 2009, 2094 |
| BseAI | TCCGGA | 1 cut(s) | 160 |
| BseBI | CCWGG | 4 cut(s) | 1290, 1301, 1960, 2632 |
| BseDI | CCNNGG | 3 cut(s) | 1300, 1893, 2645 |
| BseGI | GGATG | 7 cut(s) | 463, 709, 1062, 1066, 1504, 1789, 2627 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 2178, 2199 |
| BseMI | GCAATG | 3 cut(s) | 738, 2009, 2094 |
| BseMII | CTCAG | 4 cut(s) | 432, 551, 1270, 2781 |
| BseNI | ACTGG | 4 cut(s) | 468, 1765, 2191, 2331 |
| BseRI | GAGGAG | 4 cut(s) | 20, 48, 386, 1260 |
| BseXI | GCAGC | 5 cut(s) | 148, 284, 1900, 2422, 2568 |
| BseYI | CCCAGC | 2 cut(s) | 1948, 2198 |
| BsgI | GTGCAG | 2 cut(s) | 1950, 2406 |
| Bsh1236I | CGCG | 1 cut(s) | 698 |
| Bsh1285I | CGRYCG | 1 cut(s) | 513 |
| BshFI | GGCC | 1 cut(s) | 2171 |
| BshNI | GGYRCC | 5 cut(s) | 805, 829, 1505, 1683, 2189 |
| BshTI | ACCGGT | 1 cut(s) | 2269 |
| BsiEI | CGRYCG | 1 cut(s) | 513 |
| BsiHKAI | GWGCWC | 2 cut(s) | 168, 1164 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2644 |
| BsiSI | CCGG | 5 cut(s) | 161, 513, 611, 981, 2270 |
| BslFI | GGGAC | 4 cut(s) | 396, 984, 1971, 2041 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 2178, 2199 |
| BsmAI | GTCTC | 4 cut(s) | 731, 1941, 2372, 2744 |
| BsmFI | GGGAC | 4 cut(s) | 396, 984, 1971, 2041 |
| BsmI | GAATGC | 3 cut(s) | 674, 676, 1627 |
| BsnI | GGCC | 1 cut(s) | 2171 |
| Bso31I | GGTCTC | 1 cut(s) | 2744 |
| BsoBI | CYCGRG | 1 cut(s) | 2644 |
| Bsp119I | TTCGAA | 2 cut(s) | 106, 396 |
| Bsp1286I | GDGCHC | 3 cut(s) | 12, 168, 1164 |
| Bsp13I | TCCGGA | 1 cut(s) | 160 |
| Bsp143I | GATC | 6 cut(s) | 515, 622, 1067, 1339, 1798, 1861 |
| BspACI | CCGC | 3 cut(s) | 1380, 1509, 1663 |
| BspANI | GGCC | 1 cut(s) | 2171 |
| BspCNI | CTCAG | 4 cut(s) | 433, 552, 1269, 2780 |
| BspEI | TCCGGA | 1 cut(s) | 160 |
| BspFNI | CGCG | 1 cut(s) | 698 |
| BspMAI | CTGCAG | 1 cut(s) | 2440 |
| BspMI | ACCTGC | 2 cut(s) | 2092, 2428 |
| BspOI | GCTAGC | 1 cut(s) | 1920 |
| BspPI | GGATC | 2 cut(s) | 523, 1334 |
| BspT104I | TTCGAA | 2 cut(s) | 106, 396 |
| BspT107I | GGYRCC | 5 cut(s) | 805, 829, 1505, 1683, 2189 |
| BspTNI | GGTCTC | 1 cut(s) | 2744 |
| BsrDI | GCAATG | 3 cut(s) | 738, 2009, 2094 |
| BsrFI | RCCGGY | 1 cut(s) | 2269 |
| BsrI | ACTGG | 4 cut(s) | 468, 1765, 2191, 2331 |
| BssAI | RCCGGY | 1 cut(s) | 2269 |
| BssECI | CCNNGG | 3 cut(s) | 1300, 1893, 2645 |
| BssMI | GATC | 6 cut(s) | 515, 622, 1067, 1339, 1798, 1861 |
| BssT1I | CCWWGG | 1 cut(s) | 1893 |
| Bst2UI | CCWGG | 4 cut(s) | 1290, 1301, 1960, 2632 |
| Bst6I | CTCTTC | 2 cut(s) | 1023, 1362 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1913 |
| BstBI | TTCGAA | 2 cut(s) | 106, 396 |
| BstC8I | GCNNGC | 3 cut(s) | 674, 1584, 1918 |
| BstDEI | CTNAG | 6 cut(s) | 441, 560, 1256, 1272, 1812, 2767 |
| BstF5I | GGATG | 7 cut(s) | 463, 709, 1062, 1066, 1504, 1789, 2627 |
| BstFNI | CGCG | 1 cut(s) | 698 |
| BstHHI | GCGC | 2 cut(s) | 698, 761 |
| BstKTI | GATC | 6 cut(s) | 518, 625, 1070, 1342, 1801, 1864 |
| BstMAI | GTCTC | 4 cut(s) | 731, 1941, 2372, 2744 |
| BstMBI | GATC | 6 cut(s) | 515, 622, 1067, 1339, 1798, 1861 |
| BstMCI | CGRYCG | 1 cut(s) | 513 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 81, 1508, 1770, 1913 |
| BstNI | CCWGG | 4 cut(s) | 1290, 1301, 1960, 2632 |
| BstSCI | CCNGG | 4 cut(s) | 1288, 1299, 1958, 2630 |
| BstSFI | CTRYAG | 4 cut(s) | 600, 1650, 2118, 2436 |
| BstUI | CGCG | 1 cut(s) | 698 |
| BstV1I | GCAGC | 5 cut(s) | 148, 284, 1900, 2422, 2568 |
| BstV2I | GAAGAC | 2 cut(s) | 695, 741 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1948 |
| Bsu36I | CCTNAGG | 1 cut(s) | 441 |
| BsuI | GTATCC | 1 cut(s) | 1827 |
| BsuRI | GGCC | 1 cut(s) | 2171 |
| BtgZI | GCGATG | 3 cut(s) | 276, 888, 1416 |
| BtrI | CACGTC | 1 cut(s) | 130 |
| BtsCI | GGATG | 7 cut(s) | 463, 709, 1062, 1066, 1504, 1789, 2627 |
| BtsI | GCAGTG | 1 cut(s) | 118 |
| BtsIMutI | CAGTG | 4 cut(s) | 118, 361, 1728, 2338 |
| BveI | ACCTGC | 2 cut(s) | 2092, 2428 |
| Cac8I | GCNNGC | 3 cut(s) | 674, 1584, 1918 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2381 |
| CfoI | GCGC | 2 cut(s) | 698, 761 |
| Cfr10I | RCCGGY | 1 cut(s) | 2269 |
| Cfr13I | GGNCC | 3 cut(s) | 437, 1529, 2170 |
| CseI | GACGC | 1 cut(s) | 2304 |
| Csp6I | GTAC | 5 cut(s) | 96, 155, 1416, 2184, 2814 |
| CspAI | ACCGGT | 1 cut(s) | 2269 |
| CviAII | CATG | 8 cut(s) | 938, 1088, 1436, 1688, 1715, 1770, 1774, 2006 |
| CviQI | GTAC | 5 cut(s) | 96, 155, 1416, 2184, 2814 |
| DdeI | CTNAG | 6 cut(s) | 441, 560, 1256, 1272, 1812, 2767 |
| DpnI | GATC | 6 cut(s) | 517, 624, 1069, 1341, 1800, 1863 |
| DpnII | GATC | 6 cut(s) | 515, 622, 1067, 1339, 1798, 1861 |
| DraI | TTTAAA | 1 cut(s) | 2638 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 745, 2713 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 745, 2713 |
| Eam1104I | CTCTTC | 2 cut(s) | 1023, 1362 |
| EarI | CTCTTC | 2 cut(s) | 1023, 1362 |
| Ecl136II | GAGCTC | 1 cut(s) | 166 |
| Eco130I | CCWWGG | 1 cut(s) | 1893 |
| Eco24I | GRGCYC | 2 cut(s) | 12, 168 |
| Eco31I | GGTCTC | 1 cut(s) | 2744 |
| Eco47I | GGWCC | 2 cut(s) | 437, 1529 |
| Eco53kI | GAGCTC | 1 cut(s) | 166 |
| Eco57I | CTGAAG | 7 cut(s) | 337, 802, 807, 855, 1506, 2586, 2598 |
| Eco81I | CCTNAGG | 1 cut(s) | 441 |
| Eco88I | CYCGRG | 1 cut(s) | 2644 |
| EcoICRI | GAGCTC | 1 cut(s) | 166 |
| EcoRI | GAATTC | 2 cut(s) | 108, 1999 |
| EcoRII | CCWGG | 4 cut(s) | 1288, 1299, 1958, 2630 |
| EcoT14I | CCWWGG | 1 cut(s) | 1893 |
| EcoT22I | ATGCAT | 2 cut(s) | 1775, 2011 |
| EcoT38I | GRGCYC | 2 cut(s) | 12, 168 |
| ErhI | CCWWGG | 1 cut(s) | 1893 |
| FaeI | CATG | 8 cut(s) | 941, 1091, 1439, 1691, 1718, 1773, 1777, 2009 |
| FaqI | GGGAC | 4 cut(s) | 396, 984, 1971, 2041 |
| FatI | CATG | 8 cut(s) | 937, 1087, 1435, 1687, 1714, 1769, 1773, 2005 |
| FauNDI | CATATG | 1 cut(s) | 1008 |
| FbaI | TGATCA | 1 cut(s) | 1798 |
| FblI | GTMKAC | 3 cut(s) | 67, 509, 1285 |
| Fnu4HI | GCNGC | 7 cut(s) | 137, 273, 1509, 1663, 1914, 2436, 2582 |
| FokI | GGATG | 7 cut(s) | 470, 716, 1069, 1073, 1511, 1796, 2614 |
| FriOI | GRGCYC | 2 cut(s) | 12, 168 |
| Fsp4HI | GCNGC | 7 cut(s) | 137, 273, 1509, 1663, 1914, 2436, 2582 |
| FspBI | CTAG | 8 cut(s) | 693, 888, 1242, 1349, 1604, 1917, 2145, 2463 |
| GlaI | GCGC | 2 cut(s) | 697, 760 |
| GluI | GCNGC | 7 cut(s) | 137, 273, 1509, 1663, 1914, 2436, 2582 |
| GsaI | CCCAGC | 2 cut(s) | 1952, 2202 |
| GsuI | CTGGAG | 2 cut(s) | 485, 1311 |
| HaeIII | GGCC | 1 cut(s) | 2171 |
| HapII | CCGG | 5 cut(s) | 161, 513, 611, 981, 2270 |
| HgaI | GACGC | 1 cut(s) | 2304 |
| HhaI | GCGC | 2 cut(s) | 698, 761 |
| Hin1II | CATG | 8 cut(s) | 941, 1091, 1439, 1691, 1718, 1773, 1777, 2009 |
| Hin6I | GCGC | 2 cut(s) | 696, 759 |
| HinP1I | GCGC | 2 cut(s) | 696, 759 |
| HincII | GTYRAC | 3 cut(s) | 68, 510, 1147 |
| HindII | GTYRAC | 3 cut(s) | 68, 510, 1147 |
| HindIII | AAGCTT | 2 cut(s) | 1410, 2775 |
| HpaII | CCGG | 5 cut(s) | 161, 513, 611, 981, 2270 |
| HphI | GGTGA | 7 cut(s) | 53, 650, 1706, 1871, 2310, 2345, 2537 |
| Hpy166II | GTNNAC | 6 cut(s) | 68, 320, 510, 1147, 1286, 2802 |
| Hpy188III | TCNNGA | 7 cut(s) | 79, 161, 173, 381, 440, 1406, 2769 |
| Hpy8I | GTNNAC | 6 cut(s) | 68, 320, 510, 1147, 1286, 2802 |
| Hpy99I | CGWCG | 3 cut(s) | 69, 72, 131 |
| HpyCH4IV | ACGT | 3 cut(s) | 129, 404, 577 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 81, 1508, 1770, 1913 |
| HpyF3I | CTNAG | 6 cut(s) | 441, 560, 1256, 1272, 1812, 2767 |
| HpySE526I | ACGT | 3 cut(s) | 129, 404, 577 |
| Hsp92II | CATG | 8 cut(s) | 941, 1091, 1439, 1691, 1718, 1773, 1777, 2009 |
| HspAI | GCGC | 2 cut(s) | 696, 759 |
| Kpn2I | TCCGGA | 1 cut(s) | 160 |
| Ksp22I | TGATCA | 1 cut(s) | 1798 |
| Kzo9I | GATC | 6 cut(s) | 515, 622, 1067, 1339, 1798, 1861 |
| LmnI | GCTCC | 6 cut(s) | 7, 163, 466, 502, 1850, 2019 |
| Lsp1109I | GCAGC | 5 cut(s) | 148, 284, 1900, 2422, 2568 |
| LweI | GCATC | 6 cut(s) | 865, 1439, 1489, 1860, 2199, 2636 |
| MaeI | CTAG | 8 cut(s) | 693, 888, 1242, 1349, 1604, 1917, 2145, 2463 |
| MaeII | ACGT | 3 cut(s) | 129, 404, 577 |
| MaeIII | GTNAC | 8 cut(s) | 563, 906, 1694, 1823, 2275, 2316, 2507, 2658 |
| MalI | GATC | 6 cut(s) | 517, 624, 1069, 1341, 1800, 1863 |
| MboI | GATC | 6 cut(s) | 515, 622, 1067, 1339, 1798, 1861 |
| MfeI | CAATTG | 2 cut(s) | 1179, 2718 |
| MhlI | GDGCHC | 3 cut(s) | 12, 168, 1164 |
| MlyI | GAGTC | 4 cut(s) | 1190, 2023, 2062, 2759 |
| MmeI | TCCRAC | 2 cut(s) | 681, 745 |
| Mph1103I | ATGCAT | 2 cut(s) | 1775, 2011 |
| MroI | TCCGGA | 1 cut(s) | 160 |
| MseI | TTAA | 9 cut(s) | 414, 1119, 1611, 1730, 1836, 2394, 2486, 2637, 2781 |
| MslI | CAYNNNNRTG | 4 cut(s) | 863, 1011, 1692, 2523 |
| MspI | CCGG | 5 cut(s) | 161, 513, 611, 981, 2270 |
| MspR9I | CCNGG | 4 cut(s) | 1290, 1301, 1960, 2632 |
| MunI | CAATTG | 2 cut(s) | 1179, 2718 |
| Mva1269I | GAATGC | 3 cut(s) | 674, 676, 1627 |
| MvaI | CCWGG | 4 cut(s) | 1290, 1301, 1960, 2632 |
| MvnI | CGCG | 1 cut(s) | 698 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 81, 1508, 1770, 1913 |
| NdeI | CATATG | 1 cut(s) | 1008 |
| NdeII | GATC | 6 cut(s) | 515, 622, 1067, 1339, 1798, 1861 |
| NheI | GCTAGC | 1 cut(s) | 1916 |
| NlaIII | CATG | 8 cut(s) | 941, 1091, 1439, 1691, 1718, 1773, 1777, 2009 |
| NmuCI | GTSAC | 6 cut(s) | 906, 1694, 1823, 2275, 2316, 2658 |
| NsiI | ATGCAT | 2 cut(s) | 1775, 2011 |
| NspV | TTCGAA | 2 cut(s) | 106, 396 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1579 |
| PctI | GAATGC | 3 cut(s) | 674, 676, 1627 |
| PfeI | GAWTC | 6 cut(s) | 266, 1362, 1885, 2650, 2696, 2710 |
| PflFI | GACNNNGTC | 1 cut(s) | 1229 |
| PinAI | ACCGGT | 1 cut(s) | 2269 |
| PkrI | GCNGC | 7 cut(s) | 138, 274, 1510, 1664, 1915, 2437, 2583 |
| PleI | GAGTC | 4 cut(s) | 1190, 2023, 2062, 2759 |
| PpsI | GAGTC | 4 cut(s) | 1190, 2023, 2062, 2759 |
| PshBI | ATTAAT | 1 cut(s) | 414 |
| Psp124BI | GAGCTC | 1 cut(s) | 168 |
| Psp6I | CCWGG | 4 cut(s) | 1288, 1299, 1958, 2630 |
| PspFI | CCCAGC | 2 cut(s) | 1948, 2198 |
| PspGI | CCWGG | 4 cut(s) | 1288, 1299, 1958, 2630 |
| PspPI | GGNCC | 3 cut(s) | 437, 1529, 2170 |
| PstI | CTGCAG | 1 cut(s) | 2440 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2381 |
| PsyI | GACNNNGTC | 1 cut(s) | 1229 |
| RsaI | GTAC | 5 cut(s) | 97, 156, 1417, 2185, 2815 |
| RsaNI | GTAC | 5 cut(s) | 96, 155, 1416, 2184, 2814 |
| RseI | CAYNNNNRTG | 4 cut(s) | 863, 1011, 1692, 2523 |
| SacI | GAGCTC | 1 cut(s) | 168 |
| SalI | GTCGAC | 2 cut(s) | 66, 508 |
| SaqAI | TTAA | 9 cut(s) | 414, 1119, 1611, 1730, 1836, 2394, 2486, 2637, 2781 |
| SatI | GCNGC | 7 cut(s) | 137, 273, 1509, 1663, 1914, 2436, 2582 |
| Sau3AI | GATC | 6 cut(s) | 515, 622, 1067, 1339, 1798, 1861 |
| Sau96I | GGNCC | 3 cut(s) | 437, 1529, 2170 |
| ScaI | AGTACT | 1 cut(s) | 2185 |
| SchI | GAGTC | 4 cut(s) | 1190, 2023, 2062, 2759 |
| ScrFI | CCNGG | 4 cut(s) | 1290, 1301, 1960, 2632 |
| SduI | GDGCHC | 3 cut(s) | 12, 168, 1164 |
| SfaNI | GCATC | 6 cut(s) | 865, 1439, 1489, 1860, 2199, 2636 |
| SfcI | CTRYAG | 4 cut(s) | 600, 1650, 2118, 2436 |
| SfuI | TTCGAA | 2 cut(s) | 106, 396 |
| SgrDI | CGTCGACG | 1 cut(s) | 66 |
| SinI | GGWCC | 2 cut(s) | 437, 1529 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 863, 1011, 1692, 2523 |
| SmlI | CTYRAG | 2 cut(s) | 77, 2560 |
| SmoI | CTYRAG | 2 cut(s) | 77, 2560 |
| SsiI | CCGC | 3 cut(s) | 1380, 1509, 1663 |
| SspI | AATATT | 1 cut(s) | 2785 |
| SspMI | CTAG | 8 cut(s) | 693, 888, 1242, 1349, 1604, 1917, 2145, 2463 |
| SstI | GAGCTC | 1 cut(s) | 168 |
| StyD4I | CCNGG | 4 cut(s) | 1288, 1299, 1958, 2630 |
| StyI | CCWWGG | 1 cut(s) | 1893 |
| TaiI | ACGT | 3 cut(s) | 132, 407, 580 |
| TaqI | TCGA | 7 cut(s) | 67, 106, 396, 509, 1357, 1365, 2669 |
| TatI | WGTACW | 3 cut(s) | 1415, 2183, 2813 |
| TauI | GCSGC | 2 cut(s) | 1511, 1665 |
| TfiI | GAWTC | 6 cut(s) | 266, 1362, 1885, 2650, 2696, 2710 |
| Tru1I | TTAA | 9 cut(s) | 414, 1119, 1611, 1730, 1836, 2394, 2486, 2637, 2781 |
| Tru9I | TTAA | 9 cut(s) | 414, 1119, 1611, 1730, 1836, 2394, 2486, 2637, 2781 |
| TscAI | CASTG | 4 cut(s) | 125, 361, 1728, 2338 |
| TseFI | GTSAC | 6 cut(s) | 906, 1694, 1823, 2275, 2316, 2658 |
| TseI | GCWGC | 5 cut(s) | 136, 272, 1913, 2435, 2581 |
| Tsp45I | GTSAC | 6 cut(s) | 906, 1694, 1823, 2275, 2316, 2658 |
| TspGWI | ACGGA | 1 cut(s) | 2440 |
| TspRI | CASTG | 4 cut(s) | 125, 361, 1728, 2338 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1229 |
| VpaK11BI | GGWCC | 2 cut(s) | 437, 1529 |
| VspI | ATTAAT | 1 cut(s) | 414 |
| XapI | RAATTY | 7 cut(s) | 108, 642, 942, 1124, 1190, 1706, 1999 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1013, 1673 |
| XmiI | GTMKAC | 3 cut(s) | 67, 509, 1285 |
| XspI | CTAG | 8 cut(s) | 693, 888, 1242, 1349, 1604, 1917, 2145, 2463 |
| ZrmI | AGTACT | 1 cut(s) | 2185 |
| Zsp2I | ATGCAT | 2 cut(s) | 1775, 2011 |