Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0002885

Physical Location & Seq

Genomic Coordinates Reverse (-)

30021263 .. 30030577

Gene Structure 9315 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG185600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2364 bp

ATGCCGAGGAAGAGTAAAGCAAAGAAGGAAGAGATTTGCGAGGAGGTTTGCTTCTCTTGCAAAGATGGAGGACTCCTCTTGGTCTGCGACTACAAGGGTTGTCTTAAAGCTTATCATCCGGAATGTGCGGGAAAAGATGACTCTTTTGTGGAGACTGCACGGCGCTGGAACTGCAAATGGCATTCTTGCTTCATGTGCCATAAGGCTGCAAAGTTTCAATGCTTTTGCTGCCCACAAGCTGTATGCGGACGCTGCCTCTGTGACTCTGAGTTTGTGGTTATTAAAGTGGATAGAGGATTTTGCAATCATTGCCTGAAGCTTGCTGTACTCATAGAAGAAAACTTGGATGCTGATTCTGACGGGGGCAGAGTTGACTTCAAAGATATAAACACATTCGAGTACTTATTTAAGGAGTATTGGCAGGTAATGAAAAAGAAAGAACGCTTGTCTGCAGAACATGTCCACTCTGCGAAAAAGTTATTAAAGAAGGGAAAGAACTACGTGCGTGACTTTGATTCGAGTATAATTTGTAAGAAAGATGAAAAATTTTCAGAATCCGATGAAGAAAGTGATCTTCAGGTGTCTGATTATGATGGAGTGGAAAGTGGAGAAGAATACATGCCAGTTGCCAAAAAGAAGAGATCCAAGGGAAAACAGTGTGCAGTGAAAAGAAAAGTAGAAGCAGAGAAACGAGAATTTATGGGATGGGGATCAAAGTCTCTCCTAGAATTTCTTGCTTCTATGGGTAAAGATACATCCAAGGAGTTGTCACAGTATGAAGTCACCTCTGTGGTTACAGACTATTGTAAAGAAAACAAGCTTTTTGACCCAGAAAAGAAGAAGAAAGTAATTTGCGATGCAACCTTGCAGTCTCTTTTAGGGAGGAAAACATTGAATAAAAATAGTATATATAACCTTCTGACTTCCCATTTTGCTGACAATCTGGAAGAGTCAACGGAGGGGGATGAGAGTGGAAGTAGTTCCGAAGACAAAGATGAGAGTTTTATGGGTACCTGTAAAAGGCAGATCAAGTTGAGCCCTAAAGTACCAGATGTGCAGAAAAGCTGTTTTGCTTCCATAGTTCCTGAAAACATCAAGCTTGTGTACCTGAAGAGGAGCTTAGTGGAGGAATTGTTAAAGCAACCTGAGACTTTTGGTGAGAAAGTAACAGGATGTTTTGTGAGAGTCAAATCTGACCCAAATGATTATTCACAGAAAAATTCTCACCAGCTCTTGCAAATTAAAGGTATAAAGGAAATTTTGAGGAACGGCAGAGAGGATACAGAAATACTTATGCAATTTTTCAGCAATTTTAAAGATTTACCTGTTTCCAAGCTGTCAGATGATGACTTCTGTAAGGAAGAATGTGAAGATTTGCATCAGAGAGTGAAAGATGGCCTCCTTAAAAAGCCAACTATTGTGGAGCTTGAACAGAAGGCTAGAAGTCTACATGAGAATATAACAAAGCACTGGCTTGCAAGAGAACTGAATCTGTTGCAGAAGCGCATTGACCTGGCAAATGAAAAAGGATGGAGGAGAGAATATCCTTCTATTAAGTTAATTAATATAGCAACAAAGCTGCTACTTGAAACACCATCAGAACAATCACGGCTGTTAAATGAGGTTCCGGAAGTAATTGCAGCCATAGAAAAATGTGATCCTGTTCTTGAGTACTCCAGAAGCCAGGATAAACAAGGAAATGATGGCTCTTCCGAATCTGCCCCCAGAGAATCTCCTCAAACTCCTGGCACTGCTCTAGCCAATGGAACCTCATGGCGTGCAAATGGCAGAACAGATCCTGCAGGTTCACTGCTAGACCAGGAAAGAGACCTGGAGGGTGCAGCAGCTAAACGTAAGAGAAAGCAGGCTTCATCAAACTTTGAACAGAGCTCTAGTATAGATGGTTCAAATGGTGACCATGAGAGAGACCTGGAAGATGCAAAGGATAGATCTAGGAGAAAGAAAGTTCCTACGATTCATGACTGCAAGCAGTCTCCTTGCATTCCATCTGGTCAGGCAGCAGAACTAGAGGAACCTAAAGTAGCTCGACTAGAAAGGATGAACTGTGAAAGCACATCTGAAGTTAAGGTAATTGAGTTGAGCGATGACGATGAAGATGTAATGGTTTCAGTTAGAACACCTGCACTTGAAGTCCCAGGTTCTGATTTATGGTATTGTATGAGTCCACTTGGTGAAATGAGGGGCCCCTATAAATTGTCATTGCTAAAGCTATGGAGCGTCTCGAGTGGCAATGAATTAAGATACAAGGTTTGGAGAGTGGGTCAGAGCAAAGAGGAGGCAATCTTTCTCAATGATGCCATTCACGGAAATTTTCCAACATGTAGAAATGCTTCCAAATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GYF Plus-3 SWIB

Protein Analysis

787

Amino Acids

89.3

Weight (kDa)

6.47

Isoelectric Point (pI)

52.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SWIB	PF02201	239 - 311	1.4e-12	SWIB/MDM2 domain
SWIB_eIF2D	PF26291	240 - 296	3.1e-07	eIF2D, SWIB domain
Plus-3	PF03126	364 - 463	4.8e-17	Plus-3 domain
NERD_plant	PF25980	486 - 538	1.4e-17	Plant NERD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002885)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63700 AT5G63700
fragaria_vesca	FvH4_2g12310 FvH4_2g12310 FvH4_2g12310 FvH4_2g12310 FvH4_2g12310 FvH4_2g12310
malus_domestica	MD15G1440700.v1.1
prunus_persica	Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1 Prupe.1G582800_v2.0.a1
pyrus_communis	pycom15g38850
rosa_chinensis	RchiOBHm_Chr6g0272281 RchiOBHm_Chr6g0272291
rosa_laevigata	RLG00000013684 RLG00000013685
rosa_multiflora	Rmu_co8463891.1_g000001 Rmu_sc0004424.1_g000005
rosa_roxburghii	Rroxscaffold_7G00196450
rosa_rugosa	Rorug06G0067400
rosa_samantha	Rh6AG184100 Rh6BG187300 Rh6CG185600 Rh6DG177400
rosa_wichuraiana	Rw6G015880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2149
Acc36I	ACCTGC	3 cut(s)	412, 1793, 2149
Acc65I	GGTACC	1 cut(s)	1010
AccB1I	GGYRCC	1 cut(s)	1010
AccI	GTMKAC	1 cut(s)	1447
AccIII	TCCGGA	2 cut(s)	118, 1627
AciI	CCGC	2 cut(s)	128, 246
AclWI	GGATC	4 cut(s)	636, 718, 1652, 1790
AcsI	RAATTY	6 cut(s)	545, 695, 728, 1219, 1257, 2329
AcuI	CTGAAG	4 cut(s)	335, 560, 1130, 2100
AdeI	CACNNNGTG	1 cut(s)	2192
AfaI	GTAC	6 cut(s)	327, 401, 1012, 1047, 1106, 1673
AfiI	CCNNNNNNNGG	1 cut(s)	1020
AflIII	ACRYGT	2 cut(s)	457, 2339
AgsI	TTSAA	8 cut(s)	218, 379, 895, 1430, 1589, 1883, 1908, 2150
AjnI	CCWGG	7 cut(s)	1512, 1683, 1744, 1818, 1830, 1929, 2155
AjuI	GAANNNNNNNTTGG	2 cut(s)	1192, 1224
AleI	CACNNNNGTG	1 cut(s)	788
Alw21I	GWGCWC	1 cut(s)	1892
Alw26I	GTCTC	8 cut(s)	146, 723, 876, 1142, 1821, 1920, 1998, 2245
AlwI	GGATC	4 cut(s)	636, 718, 1652, 1790
AlwNI	CAGNNNCTG	2 cut(s)	1799, 2162
Ama87I	CYCGRG	1 cut(s)	2242
Aor13HI	TCCGGA	2 cut(s)	118, 1627
AoxI	GGCC	2 cut(s)	1396, 2203
ApaI	GGGCCC	1 cut(s)	2207
ApeKI	GCWGC	8 cut(s)	206, 228, 252, 1579, 1640, 1841, 1844, 2018
ApoI	RAATTY	6 cut(s)	545, 695, 728, 1219, 1257, 2329
ArsI	GACNNNNNNTTYG	2 cut(s)	254, 286
AseI	ATTAAT	1 cut(s)	1563
Asp700I	GAANNNNTTC	2 cut(s)	979, 2350
Asp718I	GGTACC	1 cut(s)	1010
AspLEI	GCGC	2 cut(s)	165, 1506
AspS9I	GGNCC	2 cut(s)	2203, 2204
AsuHPI	GGTGA	5 cut(s)	775, 1169, 1217, 1925, 2204
AvaI	CYCGRG	1 cut(s)	2242
BaeGI	GKGCMC	1 cut(s)	2207
BanI	GGYRCC	1 cut(s)	1010
BanII	GRGCYC	3 cut(s)	1040, 1892, 2207
BarI	GAAGNNNNNNTAC	1 cut(s)	2335
BbsI	GAAGAC	1 cut(s)	993
Bbv12I	GWGCWC	1 cut(s)	1892
BbvI	GCAGC	8 cut(s)	193, 215, 239, 1566, 1652, 1853, 1856, 2030
BccI	CCATC	9 cut(s)	59, 587, 699, 1388, 1524, 1603, 1697, 1895, 2014
BceAI	ACGGC	3 cut(s)	176, 1285, 1625
BciT130I	CCWGG	7 cut(s)	1514, 1685, 1746, 1820, 1832, 1931, 2157
BciVI	GTATCC	1 cut(s)	1273
BcoDI	GTCTC	8 cut(s)	146, 723, 876, 1142, 1821, 1920, 1998, 2245
BfaI	CTAG	8 cut(s)	725, 1440, 1757, 1814, 1893, 1953, 2027, 2051
BfmI	CTRYAG	2 cut(s)	450, 1800
BfoI	RGCGCY	1 cut(s)	166
BfuAI	ACCTGC	3 cut(s)	412, 1793, 2149
BfuI	GTATCC	1 cut(s)	1273
BglII	AGATCT	1 cut(s)	1949
BisI	GCNGC	8 cut(s)	207, 229, 253, 1580, 1641, 1842, 1845, 2019
BlsI	GCNGC	8 cut(s)	208, 230, 254, 1581, 1642, 1843, 1846, 2020
BmcAI	AGTACT	2 cut(s)	401, 1673
Bme1390I	CCNGG	7 cut(s)	1514, 1685, 1746, 1820, 1832, 1931, 2157
BmeT110I	CYCGRG	1 cut(s)	2242
BmgT120I	GGNCC	2 cut(s)	2203, 2204
BmiI	GGNNCC	7 cut(s)	1012, 1626, 1768, 2034, 2204, 2205, 2206
BmrFI	CCNGG	7 cut(s)	1514, 1685, 1746, 1820, 1832, 1931, 2157
BmsI	GCATC	5 cut(s)	337, 847, 1387, 1927, 2305
BpiI	GAAGAC	1 cut(s)	993
BplI	GAGNNNNNCTC	2 cut(s)	60, 92
BpmI	CTGGAG	2 cut(s)	1660, 1853
BpuEI	CTTGAG	1 cut(s)	1688
BsaAI	YACGTR	1 cut(s)	502
BsaBI	GATNNNNATC	2 cut(s)	709, 1377
BsaI	GGTCTC	2 cut(s)	1821, 1920
BsaJI	CCNNGG	4 cut(s)	5, 645, 759, 2155
BsaWI	WCCGGW	2 cut(s)	118, 1627
BsaXI	ACNNNNNCTCC	4 cut(s)	1118, 1148, 2266, 2296
Bsc4I	CCNNNNNNNGG	1 cut(s)	1020
Bse1I	ACTGG	2 cut(s)	623, 1475
Bse3DI	GCAATG	3 cut(s)	307, 2219, 2257
Bse8I	GATNNNNATC	2 cut(s)	709, 1377
BseAI	TCCGGA	2 cut(s)	118, 1627
BseBI	CCWGG	7 cut(s)	1514, 1685, 1746, 1820, 1832, 1931, 2157
BseDI	CCNNGG	4 cut(s)	5, 645, 759, 2155
BseGI	GGATG	8 cut(s)	115, 352, 710, 755, 970, 1178, 1535, 2064
BseJI	GATNNNNATC	2 cut(s)	709, 1377
BseLI	CCNNNNNNNGG	1 cut(s)	1020
BseMI	GCAATG	3 cut(s)	307, 2219, 2257
BseMII	CTCAG	2 cut(s)	258, 1137
BseNI	ACTGG	2 cut(s)	623, 1475
BseRI	GAGGAG	6 cut(s)	56, 65, 1129, 1549, 1725, 2309
BseSI	GKGCMC	1 cut(s)	2207
BseXI	GCAGC	8 cut(s)	193, 215, 239, 1566, 1652, 1853, 1856, 2030
BsgI	GTGCAG	5 cut(s)	141, 681, 1076, 1860, 2127
BshFI	GGCC	2 cut(s)	1398, 2205
BshNI	GGYRCC	1 cut(s)	1010
BsiHKAI	GWGCWC	1 cut(s)	1892
BsiHKCI	CYCGRG	1 cut(s)	2242
BsiSI	CCGG	2 cut(s)	119, 1628
BslFI	GGGAC	1 cut(s)	2138
BslI	CCNNNNNNNGG	1 cut(s)	1020
BsmAI	GTCTC	8 cut(s)	146, 723, 876, 1142, 1821, 1920, 1998, 2245
BsmBI	CGTCTC	1 cut(s)	2245
BsmFI	GGGAC	1 cut(s)	2138
BsmI	GAATGC	2 cut(s)	181, 2001
BsnI	GGCC	2 cut(s)	1398, 2205
Bso31I	GGTCTC	2 cut(s)	1821, 1920
BsoBI	CYCGRG	1 cut(s)	2242
Bsp120I	GGGCCC	1 cut(s)	2203
Bsp1286I	GDGCHC	3 cut(s)	1040, 1892, 2207
Bsp13I	TCCGGA	2 cut(s)	118, 1627
Bsp143I	GATC	7 cut(s)	571, 641, 710, 1026, 1657, 1795, 1949
BspACI	CCGC	2 cut(s)	128, 246
BspANI	GGCC	2 cut(s)	1398, 2205
BspCNI	CTCAG	2 cut(s)	259, 1138
BspEI	TCCGGA	2 cut(s)	118, 1627
BspHI	TCATGA	1 cut(s)	1978
BspLI	GGNNCC	7 cut(s)	1012, 1626, 1768, 2034, 2204, 2205, 2206
BspMAI	CTGCAG	2 cut(s)	454, 1804
BspMI	ACCTGC	3 cut(s)	412, 1793, 2149
BspPI	GGATC	4 cut(s)	636, 718, 1652, 1790
BspQI	GCTCTTC	1 cut(s)	1714
BspT107I	GGYRCC	1 cut(s)	1010
BspTNI	GGTCTC	2 cut(s)	1821, 1920
BsrDI	GCAATG	3 cut(s)	307, 2219, 2257
BsrI	ACTGG	2 cut(s)	623, 1475
BssECI	CCNNGG	4 cut(s)	5, 645, 759, 2155
BssMI	GATC	7 cut(s)	571, 641, 710, 1026, 1657, 1795, 1949
BssT1I	CCWWGG	2 cut(s)	645, 759
Bst2UI	CCWGG	7 cut(s)	1514, 1685, 1746, 1820, 1832, 1931, 2157
Bst4CI	ACNGT	3 cut(s)	657, 774, 2066
Bst6I	CTCTTC	6 cut(s)	5, 24, 632, 942, 1106, 1714
BstAPI	GCANNNNNTGC	1 cut(s)	309
BstBAI	YACGTR	1 cut(s)	502
BstC8I	GCNNGC	5 cut(s)	321, 1476, 1779, 1866, 1988
BstDEI	CTNAG	3 cut(s)	267, 1120, 1146
BstEII	GGTNACC	1 cut(s)	1913
BstENI	CCTNNNNNAGG	1 cut(s)	1018
BstF5I	GGATG	8 cut(s)	115, 352, 710, 755, 970, 1178, 1535, 2064
BstH2I	RGCGCY	1 cut(s)	166
BstHHI	GCGC	2 cut(s)	165, 1506
BstKTI	GATC	7 cut(s)	574, 644, 713, 1029, 1660, 1798, 1952
BstMAI	GTCTC	8 cut(s)	146, 723, 876, 1142, 1821, 1920, 1998, 2245
BstMBI	GATC	7 cut(s)	571, 641, 710, 1026, 1657, 1795, 1949
BstMWI	GCNNNNNNNGC	6 cut(s)	57, 171, 195, 228, 252, 309
BstNI	CCWGG	7 cut(s)	1514, 1685, 1746, 1820, 1832, 1931, 2157
BstNSI	RCATGY	3 cut(s)	461, 622, 2343
BstPI	GGTNACC	1 cut(s)	1913
BstSCI	CCNGG	7 cut(s)	1512, 1683, 1744, 1818, 1830, 1929, 2155
BstSFI	CTRYAG	2 cut(s)	450, 1800
BstSLI	GKGCMC	1 cut(s)	2207
BstV1I	GCAGC	8 cut(s)	193, 215, 239, 1566, 1652, 1853, 1856, 2030
BstV2I	GAAGAC	1 cut(s)	993
BstX2I	RGATCY	3 cut(s)	641, 1795, 1949
BstYI	RGATCY	3 cut(s)	641, 1795, 1949
BsuI	GTATCC	1 cut(s)	1273
BsuRI	GGCC	2 cut(s)	1398, 2205
BtgZI	GCGATG	2 cut(s)	870, 2118
BtsCI	GGATG	8 cut(s)	115, 352, 710, 755, 970, 1178, 1535, 2064
BtsI	GCAGTG	3 cut(s)	669, 1749, 1808
BtsIMutI	CAGTG	5 cut(s)	662, 669, 1468, 1749, 1808
BveI	ACCTGC	3 cut(s)	412, 1793, 2149
Cac8I	GCNNGC	5 cut(s)	321, 1476, 1779, 1866, 1988
CaiI	CAGNNNCTG	2 cut(s)	1799, 2162
CciI	TCATGA	1 cut(s)	1978
CfoI	GCGC	2 cut(s)	165, 1506
Cfr13I	GGNCC	2 cut(s)	2203, 2204
CseI	GACGC	2 cut(s)	258, 2227
Csp6I	GTAC	6 cut(s)	326, 400, 1011, 1046, 1105, 1672
CspCI	CAANNNNNGTGG	2 cut(s)	1401, 1436
CviAII	CATG	8 cut(s)	193, 458, 619, 1451, 1773, 1919, 1979, 2340
CviQI	GTAC	6 cut(s)	326, 400, 1011, 1046, 1105, 1672
DdeI	CTNAG	3 cut(s)	267, 1120, 1146
DpnI	GATC	7 cut(s)	573, 643, 712, 1028, 1659, 1797, 1951
DpnII	GATC	7 cut(s)	571, 641, 710, 1026, 1657, 1795, 1949
DraI	TTTAAA	1 cut(s)	1315
DraIII	CACNNNGTG	1 cut(s)	2192
Eam1104I	CTCTTC	6 cut(s)	5, 24, 632, 942, 1106, 1714
EarI	CTCTTC	6 cut(s)	5, 24, 632, 942, 1106, 1714
Ecl136II	GAGCTC	1 cut(s)	1890
Eco130I	CCWWGG	2 cut(s)	645, 759
Eco24I	GRGCYC	3 cut(s)	1040, 1892, 2207
Eco31I	GGTCTC	2 cut(s)	1821, 1920
Eco53kI	GAGCTC	1 cut(s)	1890
Eco57I	CTGAAG	4 cut(s)	335, 560, 1130, 2100
Eco88I	CYCGRG	1 cut(s)	2242
Eco91I	GGTNACC	1 cut(s)	1913
EcoICRI	GAGCTC	1 cut(s)	1890
EcoNI	CCTNNNNNAGG	1 cut(s)	1018
EcoO109I	RGGNCCY	2 cut(s)	2203, 2204
EcoO65I	GGTNACC	1 cut(s)	1913
EcoRII	CCWGG	7 cut(s)	1512, 1683, 1744, 1818, 1830, 1929, 2155
EcoT14I	CCWWGG	2 cut(s)	645, 759
EcoT38I	GRGCYC	3 cut(s)	1040, 1892, 2207
ErhI	CCWWGG	2 cut(s)	645, 759
Esp3I	CGTCTC	1 cut(s)	2245
FaeI	CATG	8 cut(s)	196, 461, 622, 1454, 1776, 1922, 1982, 2343
FalI	AAGNNNNNCTT	2 cut(s)	1103, 1135
FaqI	GGGAC	1 cut(s)	2138
FatI	CATG	8 cut(s)	192, 457, 618, 1450, 1772, 1918, 1978, 2339
FauI	CCCGC	1 cut(s)	121
FblI	GTMKAC	1 cut(s)	1447
Fnu4HI	GCNGC	8 cut(s)	207, 229, 253, 1580, 1641, 1842, 1845, 2019
FokI	GGATG	8 cut(s)	102, 359, 717, 742, 977, 1185, 1542, 2071
FriOI	GRGCYC	3 cut(s)	1040, 1892, 2207
Fsp4HI	GCNGC	8 cut(s)	207, 229, 253, 1580, 1641, 1842, 1845, 2019
FspBI	CTAG	8 cut(s)	725, 1440, 1757, 1814, 1893, 1953, 2027, 2051
GlaI	GCGC	2 cut(s)	164, 1505
GluI	GCNGC	8 cut(s)	207, 229, 253, 1580, 1641, 1842, 1845, 2019
GsuI	CTGGAG	2 cut(s)	1660, 1853
HaeII	RGCGCY	1 cut(s)	166
HaeIII	GGCC	2 cut(s)	1398, 2205
HapII	CCGG	2 cut(s)	119, 1628
HgaI	GACGC	2 cut(s)	258, 2227
HhaI	GCGC	2 cut(s)	165, 1506
Hin1II	CATG	8 cut(s)	196, 461, 622, 1454, 1776, 1922, 1982, 2343
Hin6I	GCGC	2 cut(s)	163, 1504
HinP1I	GCGC	2 cut(s)	163, 1504
HincII	GTYRAC	2 cut(s)	373, 954
HindII	GTYRAC	2 cut(s)	373, 954
HindIII	AAGCTT	4 cut(s)	108, 317, 818, 1097
HpaII	CCGG	2 cut(s)	119, 1628
HphI	GGTGA	5 cut(s)	775, 1169, 1217, 1925, 2204
Hpy166II	GTNNAC	7 cut(s)	373, 463, 954, 1105, 1448, 1808, 2186
Hpy188III	TCNNGA	8 cut(s)	119, 944, 1085, 1628, 1667, 1677, 1979, 2242
Hpy8I	GTNNAC	7 cut(s)	373, 463, 954, 1105, 1448, 1808, 2186
HpyAV	CCTTC	5 cut(s)	19, 481, 926, 1429, 1557
HpyCH4III	ACNGT	3 cut(s)	657, 774, 2066
HpyCH4IV	ACGT	2 cut(s)	501, 1852
HpyF10VI	GCNNNNNNNGC	6 cut(s)	57, 171, 195, 228, 252, 309
HpyF3I	CTNAG	3 cut(s)	267, 1120, 1146
HpySE526I	ACGT	2 cut(s)	501, 1852
Hsp92II	CATG	8 cut(s)	196, 461, 622, 1454, 1776, 1922, 1982, 2343
HspAI	GCGC	2 cut(s)	163, 1504
Kpn2I	TCCGGA	2 cut(s)	118, 1627
KpnI	GGTACC	1 cut(s)	1014
Kzo9I	GATC	7 cut(s)	571, 641, 710, 1026, 1657, 1795, 1949
LguI	GCTCTTC	1 cut(s)	1714
LmnI	GCTCC	3 cut(s)	1116, 1423, 2235
Lsp1109I	GCAGC	8 cut(s)	193, 215, 239, 1566, 1652, 1853, 1856, 2030
LweI	GCATC	5 cut(s)	337, 847, 1387, 1927, 2305
MaeI	CTAG	8 cut(s)	725, 1440, 1757, 1814, 1893, 1953, 2027, 2051
MaeII	ACGT	2 cut(s)	501, 1852
MaeIII	GTNAC	7 cut(s)	260, 506, 768, 781, 793, 1165, 1913
MalI	GATC	7 cut(s)	573, 643, 712, 1028, 1659, 1797, 1951
MboI	GATC	7 cut(s)	571, 641, 710, 1026, 1657, 1795, 1949
MflI	RGATCY	3 cut(s)	641, 1795, 1949
MhlI	GDGCHC	3 cut(s)	1040, 1892, 2207
MlyI	GAGTC	6 cut(s)	66, 134, 257, 959, 1194, 2191
MmeI	TCCRAC	1 cut(s)	2360
MroI	TCCGGA	2 cut(s)	118, 1627
MroXI	GAANNNNTTC	2 cut(s)	979, 2350
MslI	CAYNNNNRTG	1 cut(s)	788
MspI	CCGG	2 cut(s)	119, 1628
MspR9I	CCNGG	7 cut(s)	1514, 1685, 1746, 1820, 1832, 1931, 2157
Mva1269I	GAATGC	2 cut(s)	181, 2001
MvaI	CCWGG	7 cut(s)	1514, 1685, 1746, 1820, 1832, 1931, 2157
MwoI	GCNNNNNNNGC	6 cut(s)	57, 171, 195, 228, 252, 309
NdeII	GATC	7 cut(s)	571, 641, 710, 1026, 1657, 1795, 1949
NlaIII	CATG	8 cut(s)	196, 461, 622, 1454, 1776, 1922, 1982, 2343
NlaIV	GGNNCC	7 cut(s)	1012, 1626, 1768, 2034, 2204, 2205, 2206
NmeAIII	GCCGAG	1 cut(s)	30
NmuCI	GTSAC	5 cut(s)	260, 506, 768, 781, 1913
NspI	RCATGY	3 cut(s)	461, 622, 2343
OliI	CACNNNNGTG	1 cut(s)	788
PacI	TTAATTAA	1 cut(s)	1563
PaeR7I	CTCGAG	1 cut(s)	2242
PagI	TCATGA	1 cut(s)	1978
PaqCI	CACCTGC	1 cut(s)	2149
PciI	ACATGT	2 cut(s)	457, 2339
PciSI	GCTCTTC	1 cut(s)	1714
PctI	GAATGC	2 cut(s)	181, 2001
PdmI	GAANNNNTTC	2 cut(s)	979, 2350
PfeI	GAWTC	7 cut(s)	353, 515, 554, 1489, 1715, 1730, 1975
PkrI	GCNGC	8 cut(s)	208, 230, 254, 1581, 1642, 1843, 1846, 2020
PleI	GAGTC	6 cut(s)	66, 134, 257, 958, 1193, 2190
PpsI	GAGTC	6 cut(s)	66, 134, 257, 958, 1193, 2190
Ppu21I	YACGTR	1 cut(s)	502
PscI	ACATGT	2 cut(s)	457, 2339
PshBI	ATTAAT	1 cut(s)	1563
Psp124BI	GAGCTC	1 cut(s)	1892
Psp6I	CCWGG	7 cut(s)	1512, 1683, 1744, 1818, 1830, 1929, 2155
PspEI	GGTNACC	1 cut(s)	1913
PspGI	CCWGG	7 cut(s)	1512, 1683, 1744, 1818, 1830, 1929, 2155
PspN4I	GGNNCC	7 cut(s)	1012, 1626, 1768, 2034, 2204, 2205, 2206
PspOMI	GGGCCC	1 cut(s)	2203
PspPI	GGNCC	2 cut(s)	2203, 2204
PsrI	GAACNNNNNNTAC	2 cut(s)	1888, 1920
PstI	CTGCAG	2 cut(s)	454, 1804
PstNI	CAGNNNCTG	2 cut(s)	1799, 2162
PsuI	RGATCY	3 cut(s)	641, 1795, 1949
RsaI	GTAC	6 cut(s)	327, 401, 1012, 1047, 1106, 1673
RsaNI	GTAC	6 cut(s)	326, 400, 1011, 1046, 1105, 1672
RseI	CAYNNNNRTG	1 cut(s)	788
SacI	GAGCTC	1 cut(s)	1892
SapI	GCTCTTC	1 cut(s)	1714
SatI	GCNGC	8 cut(s)	207, 229, 253, 1580, 1641, 1842, 1845, 2019
Sau3AI	GATC	7 cut(s)	571, 641, 710, 1026, 1657, 1795, 1949
Sau96I	GGNCC	2 cut(s)	2203, 2204
SbfI	CCTGCAGG	1 cut(s)	1804
ScaI	AGTACT	2 cut(s)	401, 1673
SchI	GAGTC	6 cut(s)	66, 134, 257, 959, 1194, 2191
ScrFI	CCNGG	7 cut(s)	1514, 1685, 1746, 1820, 1832, 1931, 2157
SdaI	CCTGCAGG	1 cut(s)	1804
SduI	GDGCHC	3 cut(s)	1040, 1892, 2207
SfaNI	GCATC	5 cut(s)	337, 847, 1387, 1927, 2305
SfcI	CTRYAG	2 cut(s)	450, 1800
Sfr274I	CTCGAG	1 cut(s)	2242
SlaI	CTCGAG	1 cut(s)	2242
SmiMI	CAYNNNNRTG	1 cut(s)	788
SmlI	CTYRAG	2 cut(s)	1667, 2242
SmoI	CTYRAG	2 cut(s)	1667, 2242
Sse8387I	CCTGCAGG	1 cut(s)	1804
SsiI	CCGC	2 cut(s)	128, 246
SspMI	CTAG	8 cut(s)	725, 1440, 1757, 1814, 1893, 1953, 2027, 2051
SstI	GAGCTC	1 cut(s)	1892
StyD4I	CCNGG	7 cut(s)	1512, 1683, 1744, 1818, 1830, 1929, 2155
StyI	CCWWGG	2 cut(s)	645, 759
TaaI	ACNGT	3 cut(s)	657, 774, 2066
TaiI	ACGT	2 cut(s)	504, 1855
TaqI	TCGA	4 cut(s)	396, 518, 2047, 2243
TatI	WGTACW	3 cut(s)	325, 399, 1671
TfiI	GAWTC	7 cut(s)	353, 515, 554, 1489, 1715, 1730, 1975
TscAI	CASTG	5 cut(s)	662, 669, 1475, 1756, 1815
TseFI	GTSAC	5 cut(s)	260, 506, 768, 781, 1913
TseI	GCWGC	8 cut(s)	206, 228, 252, 1579, 1640, 1841, 1844, 2018
Tsp45I	GTSAC	5 cut(s)	260, 506, 768, 781, 1913
TspGWI	ACGGA	2 cut(s)	971, 2340
TspRI	CASTG	5 cut(s)	662, 669, 1475, 1756, 1815
VspI	ATTAAT	1 cut(s)	1563
XagI	CCTNNNNNAGG	1 cut(s)	1018
XapI	RAATTY	6 cut(s)	545, 695, 728, 1219, 1257, 2329
XceI	RCATGY	3 cut(s)	461, 622, 2343
XhoI	CTCGAG	1 cut(s)	2242
XmiI	GTMKAC	1 cut(s)	1447
XmnI	GAANNNNTTC	2 cut(s)	979, 2350
XspI	CTAG	8 cut(s)	725, 1440, 1757, 1814, 1893, 1953, 2027, 2051
ZrmI	AGTACT	2 cut(s)	401, 1673

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.