Rh6DG052200

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013603
Physical Location & Seq
Forward (+)
5529085 .. 5530595
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG052200.1

Sequence Viewer

Length: 1116 bp
ATGATCAAGTATATCGACAAAGTTGTAAGGTGCAATGTTGAAGAGCTTCATCTTAAGATAAACATACATTCACAGGAACCATTCTACGTGGAATTTCCTGTTTCTCGTTGCGAATCATTAAGGTATCTATCTGTCGAGGGCATGTTTACAAATATCCAAACACCTCTTAGCTCCCCCTCCTTTTCTAATCTTGAATGCCTGAAGTTGAAACTAGTTTGGATACCAGATGAGGGGATTTTCAAATGGATCTCCCATTCTTGCAAATGCATCAAGGAGCTAGTGTTGGAAGACGTATATGGTTTTGGTATTTGGAATATTACCATCGAAAGCTCATCTTTGAAATCGTTTAGTTTTATTTTGAATGGGGGTTCCAGTTACAATCTTATTATCACCTGTGAGAAACTTGAAGATGCATGCATCTATTTGGGGATGCCGTGTCATACAGGTGGAGCGCTATGTTTTTATGCACCAAATCTTAAAAATTTGAAATTGAGTGGACACGTGATGATGTTCCAAAACTTGAGCGAAGTCATGAGTTTAGAAAAAGCTGAGATTGAGCTAGATCCCGGGGTGATTGACTTTAACTATGTATTCAAGCTGCTTCATGGTATTCGAAGTGCTAAAGTTCTGGATTTAGATATATCTGAAAGGACCATGAAGTGTCTGGACGAGGGACGTTTAACGCCAGAACGACTACAACTACATAATGTTTGTTGTTTAAGAATTCGTGGTTTGAGCTTAATGAATGACGATGTCCCCGCTATGGTCTCCCTTTTAAAAAGAATGCCAAACTTGGCTACTTTGCGCATAGAGGCTGACCGGACTGGAAGCAGATATAGATCATCATCCGGACTGGAAGCAGATATAGATCGATCATCTTATTATGGTCATGTGTCAGACTGGGAGTTGCAAAAGCTTGCTTTACATAACCTAAAGGAGGTGACAATACAGCTTTCGACCGGAGATAATGGAATCATCTTTACACAGTATATCATCCATCACGCTCTACAATTGCAGAAAATGGTTATCTATTATTTACCGGAGCAATCTTATAAAGTCAGGAGGTACAAATGGCCAAGGGCTAAAGTAGTCTTTAATTCACGTGCGAGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

371

Amino Acids

42.65

Weight (kDa)

8.17

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 8 - 121 1.4e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1053
Acc16I TGCGCA 1 cut(s) 806
AccIII TCCGGA 1 cut(s) 848
AciI CCGC 1 cut(s) 759
AclWI GGATC 2 cut(s) 254, 557
AcoI YGGCCR 1 cut(s) 1073
AcsI RAATTY 3 cut(s) 92, 481, 723
AcuI CTGAAG 1 cut(s) 221
AcvI CACGTG 2 cut(s) 502, 1103
AfaI GTAC 1 cut(s) 1067
AfeI AGCGCT 1 cut(s) 453
AfiI CCNNNNNNNGG 3 cut(s) 230, 763, 937
AflII CTTAAG 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 499
AgsI TTSAA 9 cut(s) 41, 194, 208, 241, 340, 361, 407, 487, 595
AhlI ACTAGT 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 772
AlwI GGATC 2 cut(s) 254, 557
Ama87I CYCGRG 1 cut(s) 566
Aor13HI TCCGGA 1 cut(s) 848
Aor51HI AGCGCT 1 cut(s) 453
AoxI GGCC 1 cut(s) 1073
ApeKI GCWGC 1 cut(s) 598
ApoI RAATTY 3 cut(s) 92, 481, 723
Asp700I GAANNNNTTC 1 cut(s) 45
AspLEI GCGC 2 cut(s) 454, 807
AspS9I GGNCC 1 cut(s) 651
AsuC2I CCSGG 2 cut(s) 567, 568
AsuHPI GGTGA 3 cut(s) 382, 583, 952
AsuII TTCGAA 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 566
AvaII GGWCC 1 cut(s) 651
BalI TGGCCA 1 cut(s) 1075
BbrPI CACGTG 2 cut(s) 502, 1103
BbsI GAAGAC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 585
BccI CCATC 2 cut(s) 329, 1005
BceAI ACGGC 1 cut(s) 418
BciVI GTATCC 1 cut(s) 213
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 2 cut(s) 567, 568
BcoDI GTCTC 1 cut(s) 772
BcuI ACTAGT 1 cut(s) 211
BfaI CTAG 3 cut(s) 212, 278, 560
BfoI RGCGCY 1 cut(s) 455
BfrI CTTAAG 1 cut(s) 53
BfuI GTATCC 1 cut(s) 213
BisI GCNGC 1 cut(s) 599
BlsI GCNGC 1 cut(s) 600
Bme1390I CCNGG 2 cut(s) 567, 568
Bme18I GGWCC 1 cut(s) 651
BmeT110I CYCGRG 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 651
BmiI GGNNCC 2 cut(s) 78, 370
BmrFI CCNGG 2 cut(s) 567, 568
BmrI ACTGGG 1 cut(s) 910
BmsI GCATC 4 cut(s) 276, 400, 420, 426
BmuI ACTGGG 1 cut(s) 910
BpiI GAAGAC 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 613
BpuEI CTTGAG 1 cut(s) 541
BpuMI CCSGG 2 cut(s) 567, 568
Bsa29I ATCGAT 1 cut(s) 871
BsaAI YACGTR 3 cut(s) 88, 502, 1103
BsaBI GATNNNNATC 3 cut(s) 838, 844, 867
BsaI GGTCTC 1 cut(s) 772
BsaJI CCNNGG 3 cut(s) 566, 567, 1076
BsaWI WCCGGW 4 cut(s) 819, 848, 959, 1039
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 763, 937
Bse1I ACTGG 4 cut(s) 372, 829, 858, 905
Bse3DI GCAATG 1 cut(s) 40
Bse8I GATNNNNATC 3 cut(s) 838, 844, 867
BseAI TCCGGA 1 cut(s) 848
BseCI ATCGAT 1 cut(s) 871
BseDI CCNNGG 3 cut(s) 566, 567, 1076
BseGI GGATG 3 cut(s) 435, 845, 993
BseJI GATNNNNATC 3 cut(s) 838, 844, 867
BseLI CCNNNNNNNGG 3 cut(s) 230, 763, 937
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 4 cut(s) 372, 829, 858, 905
BseXI GCAGC 1 cut(s) 585
Bsh1285I CGRYCG 1 cut(s) 960
BshFI GGCC 1 cut(s) 1075
BshVI ATCGAT 1 cut(s) 871
BsiEI CGRYCG 1 cut(s) 960
BsiHKCI CYCGRG 1 cut(s) 566
BsiSI CCGG 5 cut(s) 567, 820, 849, 960, 1040
BslFI GGGAC 2 cut(s) 687, 740
BslI CCNNNNNNNGG 3 cut(s) 230, 763, 937
BsmAI GTCTC 1 cut(s) 772
BsmFI GGGAC 2 cut(s) 687, 740
BsmI GAATGC 2 cut(s) 200, 789
BsnI GGCC 1 cut(s) 1075
Bso31I GGTCTC 1 cut(s) 772
BsoBI CYCGRG 1 cut(s) 566
Bsp119I TTCGAA 1 cut(s) 613
Bsp13I TCCGGA 1 cut(s) 848
Bsp143I GATC 6 cut(s) 3, 246, 562, 839, 868, 872
BspACI CCGC 1 cut(s) 759
BspANI GGCC 1 cut(s) 1075
BspCNI CTCAG 1 cut(s) 541
BspDI ATCGAT 1 cut(s) 871
BspEI TCCGGA 1 cut(s) 848
BspHI TCATGA 1 cut(s) 531
BspLI GGNNCC 2 cut(s) 78, 370
BspPI GGATC 2 cut(s) 254, 557
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 613
BspTI CTTAAG 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 772
BsrDI GCAATG 1 cut(s) 40
BsrI ACTGG 4 cut(s) 372, 829, 858, 905
BssECI CCNNGG 3 cut(s) 566, 567, 1076
BssMI GATC 6 cut(s) 3, 246, 562, 839, 868, 872
BssT1I CCWWGG 1 cut(s) 1076
Bst4CI ACNGT 1 cut(s) 987
Bst6I CTCTTC 1 cut(s) 36
BstAFI CTTAAG 1 cut(s) 53
BstBAI YACGTR 3 cut(s) 88, 502, 1103
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 2 cut(s) 415, 918
BstDEI CTNAG 2 cut(s) 167, 549
BstENI CCTNNNNNAGG 1 cut(s) 935
BstF5I GGATG 3 cut(s) 435, 845, 993
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 2 cut(s) 454, 807
BstKTI GATC 6 cut(s) 6, 249, 565, 842, 871, 875
BstMAI GTCTC 1 cut(s) 772
BstMBI GATC 6 cut(s) 3, 246, 562, 839, 868, 872
BstMCI CGRYCG 1 cut(s) 960
BstNSI RCATGY 2 cut(s) 145, 417
BstSCI CCNGG 2 cut(s) 565, 566
BstV1I GCAGC 1 cut(s) 585
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 2 cut(s) 246, 562
BstYI RGATCY 2 cut(s) 246, 562
Bsu15I ATCGAT 1 cut(s) 871
BsuI GTATCC 1 cut(s) 213
BsuRI GGCC 1 cut(s) 1075
BsuTUI ATCGAT 1 cut(s) 871
BtsCI GGATG 3 cut(s) 435, 845, 993
Cac8I GCNNGC 2 cut(s) 415, 918
CciI TCATGA 1 cut(s) 531
CfoI GCGC 2 cut(s) 454, 807
Cfr13I GGNCC 1 cut(s) 651
Cfr9I CCCGGG 1 cut(s) 566
ClaI ATCGAT 1 cut(s) 871
Csp6I GTAC 1 cut(s) 1066
CviAII CATG 6 cut(s) 142, 414, 532, 605, 655, 890
CviQI GTAC 1 cut(s) 1066
DdeI CTNAG 2 cut(s) 167, 549
DpnI GATC 6 cut(s) 5, 248, 564, 841, 870, 874
DpnII GATC 6 cut(s) 3, 246, 562, 839, 868, 872
DraI TTTAAA 1 cut(s) 777
EaeI YGGCCR 1 cut(s) 1073
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 1076
Eco31I GGTCTC 1 cut(s) 772
Eco47I GGWCC 1 cut(s) 651
Eco47III AGCGCT 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 221
Eco72I CACGTG 2 cut(s) 502, 1103
Eco88I CYCGRG 1 cut(s) 566
EcoNI CCTNNNNNAGG 1 cut(s) 935
EcoRI GAATTC 1 cut(s) 723
EcoT14I CCWWGG 1 cut(s) 1076
EcoT22I ATGCAT 3 cut(s) 269, 415, 419
ErhI CCWWGG 1 cut(s) 1076
FaeI CATG 6 cut(s) 145, 417, 535, 608, 658, 893
FalI AAGNNNNNCTT 2 cut(s) 319, 351
FaqI GGGAC 2 cut(s) 687, 740
FatI CATG 6 cut(s) 141, 413, 531, 604, 654, 889
FauI CCCGC 1 cut(s) 766
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 599
FokI GGATG 3 cut(s) 442, 832, 980
Fsp4HI GCNGC 1 cut(s) 599
FspBI CTAG 3 cut(s) 212, 278, 560
FspI TGCGCA 1 cut(s) 806
GlaI GCGC 2 cut(s) 453, 806
GluI GCNGC 1 cut(s) 599
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 1 cut(s) 1075
HapII CCGG 5 cut(s) 567, 820, 849, 960, 1040
HhaI GCGC 2 cut(s) 454, 807
Hin1II CATG 6 cut(s) 145, 417, 535, 608, 658, 893
Hin6I GCGC 2 cut(s) 452, 805
HinP1I GCGC 2 cut(s) 452, 805
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 2 cut(s) 113, 972
HpaII CCGG 5 cut(s) 567, 820, 849, 960, 1040
HphI GGTGA 3 cut(s) 382, 583, 952
Hpy166II GTNNAC 2 cut(s) 147, 497
Hpy188I TCNGA 2 cut(s) 646, 898
Hpy188III TCNNGA 6 cut(s) 191, 532, 629, 665, 849, 1060
Hpy8I GTNNAC 2 cut(s) 147, 497
HpyCH4III ACNGT 1 cut(s) 987
HpyCH4IV ACGT 5 cut(s) 87, 291, 501, 676, 1102
HpyCH4V TGCA 8 cut(s) 33, 261, 267, 413, 417, 467, 910, 1015
HpyF3I CTNAG 2 cut(s) 167, 549
HpySE526I ACGT 5 cut(s) 87, 291, 501, 676, 1102
Hsp92II CATG 6 cut(s) 145, 417, 535, 608, 658, 893
HspAI GCGC 2 cut(s) 452, 805
Kpn2I TCCGGA 1 cut(s) 848
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 246, 562, 839, 868, 872
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 4 cut(s) 176, 274, 449, 1042
Lsp1109I GCAGC 1 cut(s) 585
LweI GCATC 4 cut(s) 276, 400, 420, 426
MaeI CTAG 3 cut(s) 212, 278, 560
MaeII ACGT 5 cut(s) 87, 291, 501, 676, 1102
MaeIII GTNAC 2 cut(s) 374, 940
MalI GATC 6 cut(s) 5, 248, 564, 841, 870, 874
MboI GATC 6 cut(s) 3, 246, 562, 839, 868, 872
MboII GAAGA 3 cut(s) 53, 299, 419
MfeI CAATTG 1 cut(s) 1010
MflI RGATCY 2 cut(s) 246, 562
MlsI TGGCCA 1 cut(s) 1075
MluCI AATT 6 cut(s) 92, 481, 488, 723, 1010, 1096
MluNI TGGCCA 1 cut(s) 1075
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 8 cut(s) 130, 174, 187, 223, 664, 805, 931, 1056
Mox20I TGGCCA 1 cut(s) 1075
Mph1103I ATGCAT 3 cut(s) 269, 415, 419
MroI TCCGGA 1 cut(s) 848
MroXI GAANNNNTTC 1 cut(s) 45
MscI TGGCCA 1 cut(s) 1075
MseI TTAA 9 cut(s) 54, 119, 477, 582, 680, 719, 740, 776, 1095
MslI CAYNNNNRTG 1 cut(s) 444
Msp20I TGGCCA 1 cut(s) 1075
MspCI CTTAAG 1 cut(s) 53
MspI CCGG 5 cut(s) 567, 820, 849, 960, 1040
MspR9I CCNGG 2 cut(s) 567, 568
MunI CAATTG 1 cut(s) 1010
Mva1269I GAATGC 2 cut(s) 200, 789
NciI CCSGG 2 cut(s) 567, 568
NdeII GATC 6 cut(s) 3, 246, 562, 839, 868, 872
NlaIII CATG 6 cut(s) 145, 417, 535, 608, 658, 893
NlaIV GGNNCC 2 cut(s) 78, 370
NmuCI GTSAC 1 cut(s) 940
NsbI TGCGCA 1 cut(s) 806
NsiI ATGCAT 3 cut(s) 269, 415, 419
NspI RCATGY 2 cut(s) 145, 417
NspV TTCGAA 1 cut(s) 613
PaeI GCATGC 1 cut(s) 417
PagI TCATGA 1 cut(s) 531
PciSI GCTCTTC 1 cut(s) 36
PctI GAATGC 2 cut(s) 200, 789
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 2 cut(s) 113, 972
PflFI GACNNNGTC 1 cut(s) 752
PkrI GCNGC 1 cut(s) 600
PmaCI CACGTG 2 cut(s) 502, 1103
PmlI CACGTG 2 cut(s) 502, 1103
Ppu21I YACGTR 3 cut(s) 88, 502, 1103
PsiI TTATAA 1 cut(s) 1053
PspCI CACGTG 2 cut(s) 502, 1103
PspN4I GGNNCC 2 cut(s) 78, 370
PspPI GGNCC 1 cut(s) 651
PsuI RGATCY 2 cut(s) 246, 562
PsyI GACNNNGTC 1 cut(s) 752
RsaI GTAC 1 cut(s) 1067
RsaNI GTAC 1 cut(s) 1066
RseI CAYNNNNRTG 1 cut(s) 444
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 9 cut(s) 54, 119, 477, 582, 680, 719, 740, 776, 1095
SatI GCNGC 1 cut(s) 599
Sau3AI GATC 6 cut(s) 3, 246, 562, 839, 868, 872
Sau96I GGNCC 1 cut(s) 651
ScrFI CCNGG 2 cut(s) 567, 568
SfaNI GCATC 4 cut(s) 276, 400, 420, 426
SfuI TTCGAA 1 cut(s) 613
SinI GGWCC 1 cut(s) 651
SmaI CCCGGG 1 cut(s) 568
SmiMI CAYNNNNRTG 1 cut(s) 444
SmlI CTYRAG 2 cut(s) 53, 520
SmoI CTYRAG 2 cut(s) 53, 520
SpeI ACTAGT 1 cut(s) 211
SphI GCATGC 1 cut(s) 417
Sse9I AATT 6 cut(s) 92, 481, 488, 723, 1010, 1096
SsiI CCGC 1 cut(s) 759
SspI AATATT 1 cut(s) 316
SspMI CTAG 3 cut(s) 212, 278, 560
StyD4I CCNGG 2 cut(s) 565, 566
StyI CCWWGG 1 cut(s) 1076
TaaI ACNGT 1 cut(s) 987
TaiI ACGT 5 cut(s) 90, 294, 504, 679, 1105
TaqI TCGA 6 cut(s) 15, 135, 324, 613, 871, 956
TasI AATT 6 cut(s) 92, 481, 488, 723, 1010, 1096
TfiI GAWTC 2 cut(s) 113, 972
Tru1I TTAA 9 cut(s) 54, 119, 477, 582, 680, 719, 740, 776, 1095
Tru9I TTAA 9 cut(s) 54, 119, 477, 582, 680, 719, 740, 776, 1095
TseFI GTSAC 1 cut(s) 940
TseI GCWGC 1 cut(s) 598
Tsp45I GTSAC 1 cut(s) 940
TspDTI ATGAA 4 cut(s) 38, 593, 671, 758
TspMI CCCGGG 1 cut(s) 566
Tth111I GACNNNGTC 1 cut(s) 752
Vha464I CTTAAG 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 651
XagI CCTNNNNNAGG 1 cut(s) 935
XapI RAATTY 3 cut(s) 92, 481, 723
XceI RCATGY 2 cut(s) 145, 417
XcmI CCANNNNNNNNNTGG 1 cut(s) 661
XmaI CCCGGG 1 cut(s) 566
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 3 cut(s) 212, 278, 560
Zsp2I ATGCAT 3 cut(s) 269, 415, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.