Rh6DG444400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0016028
Physical Location & Seq
Forward (+)
61902652 .. 61903680
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG444400.1

Sequence Viewer

Length: 1029 bp
ATGGATGGGCTCCGAGTCCCCGTAGCAGCAGCCCCAGCTGACAATGGCATGTATGCTTCTCTCATCAACGATTGCTCGGACTCCGGCGCAGCTCTCGACTTACAGTCCCACCTTACTAGGAAGGGCCGCCGTAACCCACCACCGCCTCTACACTTGCTCAACCGCCTGCTTCTCCGTCACGTCACTGACGGTGGTTTAGACAATGCTCACCACCTGTTCGATGAAATGCCGCTCAAGGACTTCAACTCCTGGGCCACCTTGATAGTTGCTTATGCTCACAATGCCGACCACCCGGAGGCCTTGAGATTGTTTCTGACCATGCTGCACCAGCAGGAATCTCATGTCGATATGTTTGAGTTTCCTCCATGGATCATGGCTTGCGTTGTCGACGCTTGTGCCTGCACGATGGATATGAGATTAGGGGAGCAACTCCATGGCTGCTGCCTAAAGTTGGGTCATGGTAATCATGATATGTTTGTTGCCACCTCCTTGATCAACTTTTACGGCAGATTGAGGTGCCACGAAGCTGCACACCGCGCTTCTATTGGACTCTCGCAGCCTAATGCCCTCACATGGACGGCCTTAATGATCAATAACTCTAGAGGAGAGAGGTTTTTCGACGTGATCAGTGACTTTAAGGAAATAGGGAGGGCTGGGATAAAGAAGAACACTTGTATCATCTCTTGTGTCCTCAGGGCGTGTGCTAGGATGCATGATGATGGCTTCAGCGGCAGACAAGTTCATGCCAATGCCATCAAACTTGGAGTGGAATCCCATTCTTTTGTGCACTGTGGGTTGGTGGATATGTATGGAAGAAACGGGCTACTTAGAGATGCGAAACTGGTGTTTCAGACGGTCAACGATACAACATCTACTGCGTGTTGGAATGCCATGCTTACAAGTTACTTGCGGAATGGGTTACACATCGAAGCCCTTAAGTTTCTCTATCAGATGAAGGCAGATGGGTTGCAGCCTCAAGAATATCTGCTGGATCAAGTGAGGATTGCTTGCGCTAGTAATGGTCTATAA

Protein Analysis

342

Amino Acids

38.02

Weight (kDa)

6.66

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 294 - 323 4.2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31790
fragaria_vesca FvH4_2g37080 FvH4_2g37240
rosa_chinensis RchiOBHm_Chr6g0304481
rosa_laevigata RLG00000010973
rosa_multiflora Rmu_co8157394.1_g000001 Rmu_co8174882.1_g000001 Rmu_ssc0000218.1_g000054
rosa_roxburghii Rroxscaffold_7G00163710
rosa_rugosa Rorug06G0332100
rosa_samantha Rh6AG444900 Rh6BG457500 Rh6CG457600 Rh6DG444400
rosa_wichuraiana Rw6G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 516
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 387
AccII CGCG 1 cut(s) 537
AciI CCGC 7 cut(s) 127, 143, 163, 230, 535, 729, 910
AclWI GGATC 2 cut(s) 377, 999
AcuI CTGAAG 1 cut(s) 709
AfiI CCNNNNNNNGG 3 cut(s) 295, 451, 573
AflII CTTAAG 1 cut(s) 935
AgsI TTSAA 1 cut(s) 244
AhdI GACNNNNNGTC 1 cut(s) 103
AjiI CACGTC 2 cut(s) 181, 622
AjnI CCWGG 1 cut(s) 248
AluBI AGCT 3 cut(s) 38, 92, 527
AluI AGCT 3 cut(s) 38, 92, 527
Alw21I GWGCWC 1 cut(s) 789
Alw44I GTGCAC 1 cut(s) 785
AlwI GGATC 2 cut(s) 377, 999
AoxI GGCC 4 cut(s) 124, 252, 297, 579
ApaLI GTGCAC 1 cut(s) 785
ApeKI GCWGC 9 cut(s) 26, 29, 89, 322, 438, 441, 527, 556, 970
AspLEI GCGC 3 cut(s) 89, 539, 1013
AspS9I GGNCC 2 cut(s) 124, 252
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 200
AxyI CCTNAGG 1 cut(s) 692
BaeGI GKGCMC 1 cut(s) 789
BaeI ACNNNNGTAYC 2 cut(s) 658, 691
BanI GGYRCC 1 cut(s) 516
BanII GRGCYC 1 cut(s) 12
Bbv12I GWGCWC 1 cut(s) 789
BbvI GCAGC 9 cut(s) 38, 41, 101, 309, 425, 428, 514, 568, 982
BccI CCATC 4 cut(s) 400, 713, 761, 956
BceAI ACGGC 3 cut(s) 114, 520, 594
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BciT130I CCWGG 1 cut(s) 250
BclI TGATCA 3 cut(s) 492, 588, 624
BcnI CCSGG 1 cut(s) 293
BfaI CTAG 4 cut(s) 117, 600, 705, 1014
BfrI CTTAAG 1 cut(s) 935
Bme1390I CCNGG 2 cut(s) 250, 293
BmeRI GACNNNNNGTC 1 cut(s) 103
BmgBI CACGTC 2 cut(s) 181, 622
BmgT120I GGNCC 2 cut(s) 124, 252
BmiI GGNNCC 2 cut(s) 11, 518
BmrFI CCNGG 2 cut(s) 250, 293
BmsI GCATC 2 cut(s) 699, 823
BpuEI CTTGAG 3 cut(s) 218, 322, 960
BpuMI CCSGG 1 cut(s) 293
BsaJI CCNNGG 3 cut(s) 249, 365, 433
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 295, 451, 573
Bse1I ACTGG 1 cut(s) 846
Bse21I CCTNAGG 1 cut(s) 692
BseBI CCWGG 1 cut(s) 250
BseDI CCNNGG 3 cut(s) 249, 365, 433
BseGI GGATG 2 cut(s) 10, 714
BseLI CCNNNNNNNGG 3 cut(s) 295, 451, 573
BseMII CTCAG 1 cut(s) 706
BseNI ACTGG 1 cut(s) 846
BseRI GAGGAG 1 cut(s) 618
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 9 cut(s) 38, 41, 101, 309, 425, 428, 514, 568, 982
BseYI CCCAGC 2 cut(s) 34, 653
BsgI GTGCAG 3 cut(s) 308, 385, 513
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 4 cut(s) 126, 254, 299, 581
BshNI GGYRCC 1 cut(s) 516
BsiHKAI GWGCWC 1 cut(s) 789
BsiSI CCGG 2 cut(s) 84, 293
BslFI GGGAC 2 cut(s) 2, 91
BslI CCNNNNNNNGG 3 cut(s) 295, 451, 573
BsmFI GGGAC 2 cut(s) 2, 91
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 4 cut(s) 126, 254, 299, 581
Bsp1286I GDGCHC 2 cut(s) 12, 789
Bsp143I GATC 5 cut(s) 369, 492, 588, 624, 991
Bsp19I CCATGG 2 cut(s) 365, 433
BspACI CCGC 7 cut(s) 127, 143, 163, 230, 535, 729, 910
BspANI GGCC 4 cut(s) 126, 254, 299, 581
BspCNI CTCAG 1 cut(s) 705
BspFNI CGCG 1 cut(s) 537
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 11, 518
BspPI GGATC 2 cut(s) 377, 999
BspT107I GGYRCC 1 cut(s) 516
BspTI CTTAAG 1 cut(s) 935
BsrBI CCGCTC 1 cut(s) 232
BsrI ACTGG 1 cut(s) 846
BssECI CCNNGG 3 cut(s) 249, 365, 433
BssMI GATC 5 cut(s) 369, 492, 588, 624, 991
BssT1I CCWWGG 2 cut(s) 365, 433
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 4 cut(s) 105, 191, 791, 856
BstAFI CTTAAG 1 cut(s) 935
BstC8I GCNNGC 4 cut(s) 167, 379, 400, 1009
BstDEI CTNAG 2 cut(s) 692, 827
BstDSI CCRYGG 2 cut(s) 365, 433
BstF5I GGATG 2 cut(s) 10, 714
BstFNI CGCG 1 cut(s) 537
BstHHI GCGC 3 cut(s) 89, 539, 1013
BstKTI GATC 5 cut(s) 372, 495, 591, 627, 994
BstMBI GATC 5 cut(s) 369, 492, 588, 624, 991
BstMWI GCNNNNNNNGC 5 cut(s) 35, 281, 328, 536, 729
BstNI CCWGG 1 cut(s) 250
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 248, 291
BstSLI GKGCMC 1 cut(s) 789
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 9 cut(s) 38, 41, 101, 309, 425, 428, 514, 568, 982
Bsu36I CCTNAGG 1 cut(s) 692
BsuRI GGCC 4 cut(s) 126, 254, 299, 581
BtgI CCRYGG 2 cut(s) 365, 433
BtrI CACGTC 2 cut(s) 181, 622
BtsCI GGATG 2 cut(s) 10, 714
BtsIMutI CAGTG 3 cut(s) 183, 634, 787
Cac8I GCNNGC 4 cut(s) 167, 379, 400, 1009
CciI TCATGA 1 cut(s) 466
CfoI GCGC 3 cut(s) 89, 539, 1013
Cfr13I GGNCC 2 cut(s) 124, 252
CseI GACGC 1 cut(s) 398
DdeI CTNAG 2 cut(s) 692, 827
DpnI GATC 5 cut(s) 371, 494, 590, 626, 993
DpnII GATC 5 cut(s) 369, 492, 588, 624, 991
DriI GACNNNNNGTC 1 cut(s) 103
Eam1105I GACNNNNNGTC 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 365, 433
Eco147I AGGCCT 1 cut(s) 299
Eco24I GRGCYC 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 709
Eco81I CCTNAGG 1 cut(s) 692
EcoRII CCWGG 1 cut(s) 248
EcoT14I CCWWGG 2 cut(s) 365, 433
EcoT22I ATGCAT 1 cut(s) 714
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 2 cut(s) 365, 433
FaqI GGGAC 2 cut(s) 2, 91
FbaI TGATCA 3 cut(s) 492, 588, 624
FblI GTMKAC 1 cut(s) 387
FokI GGATG 2 cut(s) 17, 721
FriOI GRGCYC 1 cut(s) 12
FspBI CTAG 4 cut(s) 117, 600, 705, 1014
GlaI GCGC 3 cut(s) 88, 538, 1012
GsaI CCCAGC 2 cut(s) 38, 657
HaeIII GGCC 4 cut(s) 126, 254, 299, 581
HapII CCGG 2 cut(s) 84, 293
HgaI GACGC 1 cut(s) 398
HhaI GCGC 3 cut(s) 89, 539, 1013
Hin6I GCGC 3 cut(s) 87, 537, 1011
HinP1I GCGC 3 cut(s) 87, 537, 1011
HincII GTYRAC 2 cut(s) 388, 859
HindII GTYRAC 2 cut(s) 388, 859
HinfI GANTC 5 cut(s) 15, 80, 335, 549, 770
HpaII CCGG 2 cut(s) 84, 293
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 3 cut(s) 388, 787, 859
Hpy188I TCNGA 5 cut(s) 14, 79, 315, 852, 951
Hpy188III TCNNGA 4 cut(s) 95, 467, 600, 977
Hpy8I GTNNAC 3 cut(s) 388, 787, 859
Hpy99I CGWCG 2 cut(s) 392, 623
HpyAV CCTTC 2 cut(s) 115, 949
HpyCH4III ACNGT 4 cut(s) 105, 191, 791, 856
HpyCH4IV ACGT 2 cut(s) 180, 621
HpyCH4V TGCA 6 cut(s) 325, 402, 530, 712, 787, 970
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 281, 328, 536, 729
HpyF3I CTNAG 2 cut(s) 692, 827
HpySE526I ACGT 2 cut(s) 180, 621
HspAI GCGC 3 cut(s) 87, 537, 1011
Ksp22I TGATCA 3 cut(s) 492, 588, 624
Kzo9I GATC 5 cut(s) 369, 492, 588, 624, 991
LmnI GCTCC 2 cut(s) 15, 424
Lsp1109I GCAGC 9 cut(s) 38, 41, 101, 309, 425, 428, 514, 568, 982
LweI GCATC 2 cut(s) 699, 823
MaeI CTAG 4 cut(s) 117, 600, 705, 1014
MaeII ACGT 2 cut(s) 180, 621
MaeIII GTNAC 6 cut(s) 131, 176, 181, 629, 902, 918
MalI GATC 5 cut(s) 371, 494, 590, 626, 993
MbiI CCGCTC 1 cut(s) 232
MboI GATC 5 cut(s) 369, 492, 588, 624, 991
MboII GAAGA 2 cut(s) 676, 825
MhlI GDGCHC 2 cut(s) 12, 789
MlyI GAGTC 3 cut(s) 24, 74, 543
MmeI TCCRAC 1 cut(s) 863
Mph1103I ATGCAT 1 cut(s) 714
MseI TTAA 3 cut(s) 584, 636, 936
MslI CAYNNNNRTG 2 cut(s) 717, 747
MspA1I CMGCKG 2 cut(s) 38, 729
MspCI CTTAAG 1 cut(s) 935
MspI CCGG 2 cut(s) 84, 293
MspR9I CCNGG 2 cut(s) 250, 293
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 1 cut(s) 250
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 5 cut(s) 35, 281, 328, 536, 729
NciI CCSGG 1 cut(s) 293
NcoI CCATGG 2 cut(s) 365, 433
NdeII GATC 5 cut(s) 369, 492, 588, 624, 991
NlaIV GGNNCC 2 cut(s) 11, 518
NmuCI GTSAC 3 cut(s) 176, 181, 629
NsiI ATGCAT 1 cut(s) 714
NspI RCATGY 1 cut(s) 52
PagI TCATGA 1 cut(s) 466
PceI AGGCCT 1 cut(s) 299
PctI GAATGC 1 cut(s) 892
PfeI GAWTC 2 cut(s) 335, 770
PleI GAGTC 3 cut(s) 23, 74, 543
PpsI GAGTC 3 cut(s) 23, 74, 543
Psp6I CCWGG 1 cut(s) 248
PspFI CCCAGC 2 cut(s) 34, 653
PspGI CCWGG 1 cut(s) 248
PspN4I GGNNCC 2 cut(s) 11, 518
PspPI GGNCC 2 cut(s) 124, 252
PvuII CAGCTG 1 cut(s) 38
RseI CAYNNNNRTG 2 cut(s) 717, 747
SalI GTCGAC 1 cut(s) 386
SaqAI TTAA 3 cut(s) 584, 636, 936
Sau3AI GATC 5 cut(s) 369, 492, 588, 624, 991
Sau96I GGNCC 2 cut(s) 124, 252
SchI GAGTC 3 cut(s) 24, 74, 543
ScrFI CCNGG 2 cut(s) 250, 293
SduI GDGCHC 2 cut(s) 12, 789
SfaNI GCATC 2 cut(s) 699, 823
SmiMI CAYNNNNRTG 2 cut(s) 717, 747
SmlI CTYRAG 4 cut(s) 233, 301, 935, 975
SmoI CTYRAG 4 cut(s) 233, 301, 935, 975
SseBI AGGCCT 1 cut(s) 299
SsiI CCGC 7 cut(s) 127, 143, 163, 230, 535, 729, 910
SspMI CTAG 4 cut(s) 117, 600, 705, 1014
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 248, 291
StyI CCWWGG 2 cut(s) 365, 433
TaaI ACNGT 4 cut(s) 105, 191, 791, 856
TaiI ACGT 2 cut(s) 183, 624
TaqI TCGA 6 cut(s) 96, 219, 345, 387, 618, 927
TauI GCSGC 3 cut(s) 129, 232, 732
TfiI GAWTC 2 cut(s) 335, 770
Tru1I TTAA 3 cut(s) 584, 636, 936
Tru9I TTAA 3 cut(s) 584, 636, 936
TscAI CASTG 3 cut(s) 190, 634, 794
TseFI GTSAC 3 cut(s) 176, 181, 629
TseI GCWGC 9 cut(s) 26, 29, 89, 322, 438, 441, 527, 556, 970
Tsp45I GTSAC 3 cut(s) 176, 181, 629
TspDTI ATGAA 3 cut(s) 237, 731, 968
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 3 cut(s) 190, 634, 794
Vha464I CTTAAG 1 cut(s) 935
VneI GTGCAC 1 cut(s) 785
XbaI TCTAGA 1 cut(s) 599
XceI RCATGY 1 cut(s) 52
XmiI GTMKAC 1 cut(s) 387
XspI CTAG 4 cut(s) 117, 600, 705, 1014
Zsp2I ATGCAT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.