Rw6G038620

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016028
Physical Location & Seq
Forward (+)
62693630 .. 62695326
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G038620.1

Sequence Viewer

Length: 1323 bp
ATGGGAACCGCAGCACCTCAACAATACACATACCAATGGCAATGCCACTCTAGATTCTCAGCTACCAACCACAACAACCCGTCCATATCCAAAGTCAGGTTACCCCTCCCGCGCAGCTCAAAATCAAACACTCTTGCCTCCAACAATACTACAATTCATCCAATTCCAACACCGCCGGTTAAACCCCACCGGAATAGGAATAATAATGATGATATTAGGAAGAGGAAGTACAAGAAGAAGAAGAAGAATGGATGTGGGTCCAGCTGCAGCACCTCCGATATTCTGCGGCTGATGGATGGGCTCCGAGTCCCCGTAGCAGCAGCAGCCCCAGCTGACAATGGCATGTATGCTTCTCTCATCAACGATTGCTCGGACTCCGGCGCAGCTCTCGACTTACAGGCCCACCTTACTAGGAAGGGCCGCCGTAACCCACCACCGCCTCTACACTTGCTCAACCGCCTGCTTCTCCGTCACGTCGCTAACGGTCGTTTAGACAATGCTCACCACCTGTTCGATGAAATGCCGCTTAAGGACTTCAACTCCTGGGCCACCTTGATAGTTGCTTATGCTCACAATGGTGACTACCCGGAAGCTTTGAGATTGTTTCTGACCATGCTATACCAGCAGGAATATCATGTCGATATGTTTGAGTTTCCTACATGGATCATGGCTTGCGTTGTCGACGCTTGTGCCTGCACGATGGATATGAGATTAGGGGAGCAACTCCATGGCTGCTGCCTAAAGTTGGGTCATGGTAATCATGATATGTTTGTTGCCACCTCCTTGATCAACTTTTACGGCAGATTGAGGTGCCACGAAGCTGCACACCGCGCTTCTATTGGACTCTCGCAGCCTAATGCCCTCACATGGACGGCTATAATGATCAATAACTCTAGAGGAGAGAGGTTTTTCGACTTGATCAGTGACTTTAAGGAAATAGGGAGGGCTGGGATAAAGAAGAACACTTGTATCATCTCTTGTGTCCTCAGGGCGTGTGCTAGGATGCATGATGATGGCTTCAGCGGCAGACAAGTTCATGCCAATGCCATCAAACTTGGAGTGGAATCCCATTCTTTCGTGCACTGTGGGTTGGTGGATATGTATGGAAGAAACGGGCTACTTAGAGATGCGAAACTGGTGTTTCAGACGGTCAACGATACAACATCTACTGCGTGTTGGAATGCCATGCTTACAAGTTACTTGCGGAATGGGTTACACATCGAAGCCCTTAAGTTTCTGTATCAGATGAAGGCAGATGGGTTGCAGCCTCAAGAATATCTGCTGGATCAAGTGAGGATTGCTTGCGCTAGTAATGGTCTATAA

Protein Analysis

440

Amino Acids

49.25

Weight (kDa)

9.12

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 392 - 421 5.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31790
fragaria_vesca FvH4_2g37080 FvH4_2g37240
rosa_chinensis RchiOBHm_Chr6g0304481
rosa_laevigata RLG00000010973
rosa_multiflora Rmu_co8157394.1_g000001 Rmu_co8174882.1_g000001 Rmu_ssc0000218.1_g000054
rosa_roxburghii Rroxscaffold_7G00163710
rosa_rugosa Rorug06G0332100
rosa_samantha Rh6AG444900 Rh6BG457500 Rh6CG457600 Rh6DG444400
rosa_wichuraiana Rw6G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 810
AccI GTMKAC 1 cut(s) 681
AccII CGCG 2 cut(s) 112, 831
AclWI GGATC 2 cut(s) 671, 1293
AcuI CTGAAG 1 cut(s) 1003
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 3 cut(s) 96, 745, 867
AflII CTTAAG 2 cut(s) 527, 1229
AgsI TTSAA 1 cut(s) 538
AjiI CACGTC 1 cut(s) 475
AjnI CCWGG 1 cut(s) 542
AleI CACNNNNGTG 1 cut(s) 576
AluBI AGCT 7 cut(s) 62, 117, 264, 332, 386, 593, 821
AluI AGCT 7 cut(s) 62, 117, 264, 332, 386, 593, 821
Alw21I GWGCWC 1 cut(s) 1083
Alw44I GTGCAC 1 cut(s) 1079
AlwI GGATC 2 cut(s) 671, 1293
AoxI GGCC 3 cut(s) 399, 418, 546
ApaLI GTGCAC 1 cut(s) 1079
AspLEI GCGC 4 cut(s) 114, 383, 833, 1307
AspS9I GGNCC 4 cut(s) 258, 400, 418, 546
AsuC2I CCSGG 1 cut(s) 587
AsuHPI GGTGA 2 cut(s) 494, 590
AvaII GGWCC 1 cut(s) 258
AxyI CCTNAGG 1 cut(s) 986
BaeGI GKGCMC 1 cut(s) 1083
BaeI ACNNNNGTAYC 2 cut(s) 952, 985
BanI GGYRCC 1 cut(s) 810
BanII GRGCYC 1 cut(s) 303
BarI GAAGNNNNNNTAC 2 cut(s) 212, 244
Bbv12I GWGCWC 1 cut(s) 1083
BccI CCATC 6 cut(s) 286, 290, 694, 1007, 1055, 1250
BceAI ACGGC 3 cut(s) 408, 814, 888
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciT130I CCWGG 1 cut(s) 544
BclI TGATCA 3 cut(s) 786, 882, 918
BcnI CCSGG 1 cut(s) 587
BfaI CTAG 5 cut(s) 51, 411, 894, 999, 1308
BfmI CTRYAG 1 cut(s) 265
BfrI CTTAAG 2 cut(s) 527, 1229
Bme1390I CCNGG 2 cut(s) 544, 587
Bme18I GGWCC 1 cut(s) 258
BmgBI CACGTC 1 cut(s) 475
BmgT120I GGNCC 4 cut(s) 258, 400, 418, 546
BmiI GGNNCC 4 cut(s) 7, 259, 302, 812
BmrFI CCNGG 2 cut(s) 544, 587
BmsI GCATC 2 cut(s) 993, 1117
BpuEI CTTGAG 1 cut(s) 1254
BpuMI CCSGG 1 cut(s) 587
BsaJI CCNNGG 2 cut(s) 543, 727
BsaWI WCCGGW 1 cut(s) 189
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 745, 867
Bse118I RCCGGY 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 1140
Bse21I CCTNAGG 1 cut(s) 986
Bse3DI GCAATG 1 cut(s) 47
BseBI CCWGG 1 cut(s) 544
BseDI CCNNGG 2 cut(s) 543, 727
BseGI GGATG 4 cut(s) 157, 257, 301, 1008
BseLI CCNNNNNNNGG 3 cut(s) 96, 745, 867
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 2 cut(s) 72, 1000
BseNI ACTGG 1 cut(s) 1140
BseRI GAGGAG 1 cut(s) 912
BseSI GKGCMC 1 cut(s) 1083
BseYI CCCAGC 2 cut(s) 328, 947
BsgI GTGCAG 2 cut(s) 679, 807
Bsh1236I CGCG 2 cut(s) 112, 831
Bsh1285I CGRYCG 1 cut(s) 487
BshFI GGCC 3 cut(s) 401, 420, 548
BshNI GGYRCC 1 cut(s) 810
BsiEI CGRYCG 1 cut(s) 487
BsiHKAI GWGCWC 1 cut(s) 1083
BsiSI CCGG 4 cut(s) 176, 190, 378, 587
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 3 cut(s) 96, 745, 867
BsmFI GGGAC 1 cut(s) 293
BsmI GAATGC 1 cut(s) 1186
BsnI GGCC 3 cut(s) 401, 420, 548
Bsp1286I GDGCHC 2 cut(s) 303, 1083
Bsp143I GATC 5 cut(s) 663, 786, 882, 918, 1285
Bsp19I CCATGG 1 cut(s) 727
BspANI GGCC 3 cut(s) 401, 420, 548
BspCNI CTCAG 2 cut(s) 71, 999
BspFNI CGCG 2 cut(s) 112, 831
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 4 cut(s) 7, 259, 302, 812
BspMAI CTGCAG 1 cut(s) 269
BspPI GGATC 2 cut(s) 671, 1293
BspT107I GGYRCC 1 cut(s) 810
BspTI CTTAAG 2 cut(s) 527, 1229
BsrDI GCAATG 1 cut(s) 47
BsrFI RCCGGY 1 cut(s) 175
BsrI ACTGG 1 cut(s) 1140
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 543, 727
BssMI GATC 5 cut(s) 663, 786, 882, 918, 1285
BssT1I CCWWGG 1 cut(s) 727
Bst2UI CCWGG 1 cut(s) 544
Bst4CI ACNGT 3 cut(s) 485, 1085, 1150
Bst6I CTCTTC 1 cut(s) 215
BstAFI CTTAAG 2 cut(s) 527, 1229
BstC8I GCNNGC 4 cut(s) 461, 673, 694, 1303
BstDEI CTNAG 3 cut(s) 58, 986, 1121
BstDSI CCRYGG 1 cut(s) 727
BstEII GGTNACC 1 cut(s) 99
BstF5I GGATG 4 cut(s) 157, 257, 301, 1008
BstFNI CGCG 2 cut(s) 112, 831
BstHHI GCGC 4 cut(s) 114, 383, 833, 1307
BstKTI GATC 5 cut(s) 666, 789, 885, 921, 1288
BstMBI GATC 5 cut(s) 663, 786, 882, 918, 1285
BstMCI CGRYCG 1 cut(s) 487
BstMWI GCNNNNNNNGC 5 cut(s) 323, 329, 622, 830, 1023
BstNI CCWGG 1 cut(s) 544
BstNSI RCATGY 1 cut(s) 346
BstPI GGTNACC 1 cut(s) 99
BstSCI CCNGG 2 cut(s) 542, 585
BstSFI CTRYAG 1 cut(s) 265
BstSLI GKGCMC 1 cut(s) 1083
BstUI CGCG 2 cut(s) 112, 831
Bsu36I CCTNAGG 1 cut(s) 986
BsuRI GGCC 3 cut(s) 401, 420, 548
BtgI CCRYGG 1 cut(s) 727
BtrI CACGTC 1 cut(s) 475
BtsCI GGATG 4 cut(s) 157, 257, 301, 1008
BtsIMutI CAGTG 2 cut(s) 928, 1081
Cac8I GCNNGC 4 cut(s) 461, 673, 694, 1303
CciI TCATGA 1 cut(s) 760
CfoI GCGC 4 cut(s) 114, 383, 833, 1307
Cfr10I RCCGGY 1 cut(s) 175
Cfr13I GGNCC 4 cut(s) 258, 400, 418, 546
CseI GACGC 1 cut(s) 692
Csp6I GTAC 1 cut(s) 229
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 3 cut(s) 58, 986, 1121
DpnI GATC 5 cut(s) 665, 788, 884, 920, 1287
DpnII GATC 5 cut(s) 663, 786, 882, 918, 1285
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 727
Eco24I GRGCYC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 258
Eco57I CTGAAG 1 cut(s) 1003
Eco81I CCTNAGG 1 cut(s) 986
Eco91I GGTNACC 1 cut(s) 99
EcoO65I GGTNACC 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 542
EcoT14I CCWWGG 1 cut(s) 727
EcoT22I ATGCAT 1 cut(s) 1008
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 727
FaqI GGGAC 1 cut(s) 293
FauI CCCGC 1 cut(s) 117
FbaI TGATCA 3 cut(s) 786, 882, 918
FblI GTMKAC 1 cut(s) 681
FokI GGATG 4 cut(s) 144, 264, 308, 1015
FriOI GRGCYC 1 cut(s) 303
FspBI CTAG 5 cut(s) 51, 411, 894, 999, 1308
GlaI GCGC 4 cut(s) 113, 382, 832, 1306
GsaI CCCAGC 2 cut(s) 332, 951
HaeIII GGCC 3 cut(s) 401, 420, 548
HapII CCGG 4 cut(s) 176, 190, 378, 587
HgaI GACGC 1 cut(s) 692
HhaI GCGC 4 cut(s) 114, 383, 833, 1307
Hin6I GCGC 4 cut(s) 112, 381, 831, 1305
HinP1I GCGC 4 cut(s) 112, 381, 831, 1305
HincII GTYRAC 2 cut(s) 682, 1153
HindII GTYRAC 2 cut(s) 682, 1153
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 5 cut(s) 54, 306, 374, 843, 1064
HpaII CCGG 4 cut(s) 176, 190, 378, 587
HphI GGTGA 2 cut(s) 494, 590
Hpy166II GTNNAC 3 cut(s) 682, 1081, 1153
Hpy188I TCNGA 6 cut(s) 277, 305, 373, 609, 1146, 1245
Hpy188III TCNNGA 5 cut(s) 51, 389, 761, 894, 1271
Hpy8I GTNNAC 3 cut(s) 682, 1081, 1153
Hpy99I CGWCG 2 cut(s) 479, 686
HpyAV CCTTC 2 cut(s) 409, 1243
HpyCH4III ACNGT 3 cut(s) 485, 1085, 1150
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 6 cut(s) 267, 696, 824, 1006, 1081, 1264
HpyF10VI GCNNNNNNNGC 5 cut(s) 323, 329, 622, 830, 1023
HpyF3I CTNAG 3 cut(s) 58, 986, 1121
HpySE526I ACGT 1 cut(s) 474
HspAI GCGC 4 cut(s) 112, 381, 831, 1305
Ksp22I TGATCA 3 cut(s) 786, 882, 918
Kzo9I GATC 5 cut(s) 663, 786, 882, 918, 1285
LmnI GCTCC 2 cut(s) 306, 718
LweI GCATC 2 cut(s) 993, 1117
MaeI CTAG 5 cut(s) 51, 411, 894, 999, 1308
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 7 cut(s) 99, 425, 470, 578, 923, 1196, 1212
MalI GATC 5 cut(s) 665, 788, 884, 920, 1287
MboI GATC 5 cut(s) 663, 786, 882, 918, 1285
MboII GAAGA 7 cut(s) 232, 247, 250, 253, 256, 970, 1119
MhlI GDGCHC 2 cut(s) 303, 1083
MluCI AATT 2 cut(s) 153, 162
MlyI GAGTC 3 cut(s) 315, 368, 837
MmeI TCCRAC 3 cut(s) 165, 191, 1157
Mph1103I ATGCAT 1 cut(s) 1008
MseI TTAA 4 cut(s) 180, 528, 930, 1230
MslI CAYNNNNRTG 4 cut(s) 34, 576, 1011, 1041
MspA1I CMGCKG 3 cut(s) 264, 332, 1023
MspCI CTTAAG 2 cut(s) 527, 1229
MspI CCGG 4 cut(s) 176, 190, 378, 587
MspR9I CCNGG 2 cut(s) 544, 587
Mva1269I GAATGC 1 cut(s) 1186
MvaI CCWGG 1 cut(s) 544
MvnI CGCG 2 cut(s) 112, 831
MwoI GCNNNNNNNGC 5 cut(s) 323, 329, 622, 830, 1023
NciI CCSGG 1 cut(s) 587
NcoI CCATGG 1 cut(s) 727
NdeII GATC 5 cut(s) 663, 786, 882, 918, 1285
NlaIV GGNNCC 4 cut(s) 7, 259, 302, 812
NmuCI GTSAC 3 cut(s) 470, 578, 923
NsiI ATGCAT 1 cut(s) 1008
NspI RCATGY 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 576
PagI TCATGA 1 cut(s) 760
PctI GAATGC 1 cut(s) 1186
PfeI GAWTC 2 cut(s) 54, 1064
PleI GAGTC 3 cut(s) 314, 368, 837
PpsI GAGTC 3 cut(s) 314, 368, 837
Psp6I CCWGG 1 cut(s) 542
PspEI GGTNACC 1 cut(s) 99
PspFI CCCAGC 2 cut(s) 328, 947
PspGI CCWGG 1 cut(s) 542
PspN4I GGNNCC 4 cut(s) 7, 259, 302, 812
PspPI GGNCC 4 cut(s) 258, 400, 418, 546
PstI CTGCAG 1 cut(s) 269
PvuII CAGCTG 2 cut(s) 264, 332
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 4 cut(s) 34, 576, 1011, 1041
SalI GTCGAC 1 cut(s) 680
SaqAI TTAA 4 cut(s) 180, 528, 930, 1230
Sau3AI GATC 5 cut(s) 663, 786, 882, 918, 1285
Sau96I GGNCC 4 cut(s) 258, 400, 418, 546
SchI GAGTC 3 cut(s) 315, 368, 837
ScrFI CCNGG 2 cut(s) 544, 587
SduI GDGCHC 2 cut(s) 303, 1083
SfaNI GCATC 2 cut(s) 993, 1117
SfcI CTRYAG 1 cut(s) 265
SinI GGWCC 1 cut(s) 258
SmiMI CAYNNNNRTG 4 cut(s) 34, 576, 1011, 1041
SmlI CTYRAG 3 cut(s) 527, 1229, 1269
SmoI CTYRAG 3 cut(s) 527, 1229, 1269
Sse9I AATT 2 cut(s) 153, 162
SspMI CTAG 5 cut(s) 51, 411, 894, 999, 1308
StyD4I CCNGG 2 cut(s) 542, 585
StyI CCWWGG 1 cut(s) 727
TaaI ACNGT 3 cut(s) 485, 1085, 1150
TaiI ACGT 1 cut(s) 477
TaqI TCGA 6 cut(s) 390, 513, 639, 681, 912, 1221
TasI AATT 2 cut(s) 153, 162
TatI WGTACW 1 cut(s) 228
TauI GCSGC 4 cut(s) 289, 423, 526, 1026
TfiI GAWTC 2 cut(s) 54, 1064
Tru1I TTAA 4 cut(s) 180, 528, 930, 1230
Tru9I TTAA 4 cut(s) 180, 528, 930, 1230
TscAI CASTG 2 cut(s) 928, 1088
TseFI GTSAC 3 cut(s) 470, 578, 923
Tsp45I GTSAC 3 cut(s) 470, 578, 923
TspDTI ATGAA 4 cut(s) 146, 531, 1025, 1262
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 2 cut(s) 928, 1088
Vha464I CTTAAG 2 cut(s) 527, 1229
VneI GTGCAC 1 cut(s) 1079
VpaK11BI GGWCC 1 cut(s) 258
XbaI TCTAGA 2 cut(s) 50, 893
XceI RCATGY 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 681
XspI CTAG 5 cut(s) 51, 411, 894, 999, 1308
Zsp2I ATGCAT 1 cut(s) 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.