Rh7CG047000

NEP1-interacting protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0007345
Physical Location & Seq
Reverse (-)
3199520 .. 3201755
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG047000.1

Sequence Viewer

Length: 645 bp
ATGGAGAGCGGAGAGATTCAGAGGGTTGGAGTCCTTCAACGCCTTCCACTGCTTGTGATCAACTTCGTTGCTGGGACTCTGGTTCTGCTTTTTGCCCTTGCGGGATTTTTCGCAGGAGGCATTTTTGGTGCTTTAGCCGGTAAGGCTTCTGACAGTGGTATTGTTCGTGGAGCTGGATTGGGTGCTGTTGCTGGGGCTGTTCTCTCTGTGGAGATTTTGGAAGCTTCACGTGAGTTATTTCGTCTTGGTCGGCCTGGCTCACGAAGATCTTCATTGATGGCAGAAATTACAGAGGAGCTTATTTGTTGGAGGTTTGAGGAGGAAAACTTACCACCGCCAGAAGTGCTAAGTGCTAGCCATTGGCAGCAGGTTAACTTTGCTAATCTTAACCTTGATGAGATTCGTGATGGAGTAGTTGAAACCAGAGGGTTGTCAAGGGATTTGTTCAAGAAATTGCCATATCATGAGATTCTGGGGGATACCAAGGCAGCTCGGGTTTCCTGTTGTACGATATGTTTGCAGGATCTTGAAGTGGGAGAAACTGTAAGGACCCTGCCACTCTGTCAGCATACATTTCACTTGGCTTGTGTGGACAAGTGGCTTGTTAGACATGGTTCATGCCCTTTATGCAGACAAGATGTGTGA

Protein Analysis

214

Amino Acids

23.37

Weight (kDa)

5.76

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 167 - 211 1.4e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 168 - 211 1.6e-13 Ring finger domain
zf-RING_11 PF17123 169 - 197 2e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 169 - 210 1.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 169 - 208 9.2e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 358
AccBSI CCGCTC 1 cut(s) 9
AciI CCGC 3 cut(s) 9, 101, 335
AclWI GGATC 1 cut(s) 531
AcvI CACGTG 1 cut(s) 230
AfaI GTAC 1 cut(s) 508
AgsI TTSAA 4 cut(s) 38, 419, 448, 530
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 4 cut(s) 173, 224, 298, 491
AluI AGCT 4 cut(s) 173, 224, 298, 491
AlwI GGATC 1 cut(s) 531
Ama87I CYCGRG 1 cut(s) 492
AoxI GGCC 1 cut(s) 251
ApeKI GCWGC 2 cut(s) 364, 488
Asp700I GAANNNNTTC 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 549
AsuNHI GCTAGC 1 cut(s) 353
AvaI CYCGRG 1 cut(s) 492
AvaII GGWCC 1 cut(s) 549
BbrPI CACGTG 1 cut(s) 230
BbvI GCAGC 2 cut(s) 376, 500
BccI CCATC 2 cut(s) 271, 401
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciT130I CCWGG 1 cut(s) 255
BciVI GTATCC 1 cut(s) 472
BclI TGATCA 1 cut(s) 57
BfaI CTAG 1 cut(s) 354
BfuAI ACCTGC 1 cut(s) 358
BfuI GTATCC 1 cut(s) 472
BglI GCCNNNNNGGC 1 cut(s) 143
BglII AGATCT 1 cut(s) 266
BisI GCNGC 2 cut(s) 365, 489
BlsI GCNGC 2 cut(s) 366, 490
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 1 cut(s) 549
BmeT110I CYCGRG 1 cut(s) 492
BmgT120I GGNCC 1 cut(s) 549
BmiI GGNNCC 1 cut(s) 551
BmrFI CCNGG 1 cut(s) 255
BmtI GCTAGC 1 cut(s) 357
BsaAI YACGTR 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 483
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bse118I RCCGGY 1 cut(s) 137
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 483
BseRI GAGGAG 2 cut(s) 308, 332
BseXI GCAGC 2 cut(s) 376, 500
BseYI CCCAGC 2 cut(s) 71, 191
BshFI GGCC 1 cut(s) 253
BsiHKCI CYCGRG 1 cut(s) 492
BsiSI CCGG 1 cut(s) 138
BslFI GGGAC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 1 cut(s) 253
BsoBI CYCGRG 1 cut(s) 492
Bsp143I GATC 3 cut(s) 57, 266, 523
BspACI CCGC 3 cut(s) 9, 101, 335
BspANI GGCC 1 cut(s) 253
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 1 cut(s) 551
BspMI ACCTGC 1 cut(s) 358
BspOI GCTAGC 1 cut(s) 357
BspPI GGATC 1 cut(s) 531
BsrBI CCGCTC 1 cut(s) 9
BsrFI RCCGGY 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 3 cut(s) 57, 266, 523
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 2 cut(s) 155, 544
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 1 cut(s) 347
BstKTI GATC 3 cut(s) 60, 269, 526
BstMBI GATC 3 cut(s) 57, 266, 523
BstMWI GCNNNNNNNGC 3 cut(s) 143, 343, 627
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
BstV1I GCAGC 2 cut(s) 376, 500
BstX2I RGATCY 2 cut(s) 266, 523
BstYI RGATCY 2 cut(s) 266, 523
BsuI GTATCC 1 cut(s) 472
BsuRI GGCC 1 cut(s) 253
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 2 cut(s) 47, 160
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 1 cut(s) 355
CciI TCATGA 1 cut(s) 463
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 549
Csp6I GTAC 1 cut(s) 507
CviAII CATG 3 cut(s) 464, 611, 618
CviQI GTAC 1 cut(s) 507
DdeI CTNAG 1 cut(s) 347
DpnI GATC 3 cut(s) 59, 268, 525
DpnII GATC 3 cut(s) 57, 266, 523
Eco130I CCWWGG 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 549
Eco72I CACGTG 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 492
EcoO109I RGGNCCY 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 3 cut(s) 467, 614, 621
FaiI YATR 7 cut(s) 460, 465, 514, 570, 612, 619, 628
FaqI GGGAC 1 cut(s) 88
FatI CATG 3 cut(s) 463, 610, 617
FauI CCCGC 1 cut(s) 94
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 2 cut(s) 365, 489
Fsp4HI GCNGC 2 cut(s) 365, 489
FspBI CTAG 1 cut(s) 354
GluI GCNGC 2 cut(s) 365, 489
GsaI CCCAGC 2 cut(s) 75, 195
HaeIII GGCC 1 cut(s) 253
HapII CCGG 1 cut(s) 138
Hin1II CATG 3 cut(s) 467, 614, 621
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 222
HinfI GANTC 5 cut(s) 16, 30, 76, 400, 469
HpaI GTTAAC 1 cut(s) 373
HpaII CCGG 1 cut(s) 138
Hpy166II GTNNAC 2 cut(s) 373, 592
Hpy188I TCNGA 2 cut(s) 21, 151
Hpy188III TCNNGA 5 cut(s) 261, 404, 448, 464, 527
Hpy8I GTNNAC 2 cut(s) 373, 592
HpyAV CCTTC 2 cut(s) 44, 53
HpyCH4III ACNGT 2 cut(s) 155, 544
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 2 cut(s) 520, 630
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 343, 627
HpyF3I CTNAG 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 3 cut(s) 467, 614, 621
Ksp22I TGATCA 1 cut(s) 57
KspAI GTTAAC 1 cut(s) 373
Kzo9I GATC 3 cut(s) 57, 266, 523
LmnI GCTCC 2 cut(s) 170, 295
Lsp1109I GCAGC 2 cut(s) 376, 500
MaeI CTAG 1 cut(s) 354
MaeII ACGT 1 cut(s) 229
MalI GATC 3 cut(s) 59, 268, 525
MbiI CCGCTC 1 cut(s) 9
MboI GATC 3 cut(s) 57, 266, 523
MboII GAAGA 2 cut(s) 261, 276
MflI RGATCY 2 cut(s) 266, 523
MluCI AATT 2 cut(s) 285, 452
MlyI GAGTC 2 cut(s) 39, 70
MmeI TCCRAC 2 cut(s) 7, 287
MnlI CCTC 7 cut(s) 15, 110, 286, 303, 310, 313, 419
MroXI GAANNNNTTC 1 cut(s) 268
MseI TTAA 2 cut(s) 372, 387
MspI CCGG 1 cut(s) 138
MspR9I CCNGG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 3 cut(s) 143, 343, 627
NdeII GATC 3 cut(s) 57, 266, 523
NheI GCTAGC 1 cut(s) 353
NlaIII CATG 3 cut(s) 467, 614, 621
NlaIV GGNNCC 1 cut(s) 551
PagI TCATGA 1 cut(s) 463
PdmI GAANNNNTTC 1 cut(s) 268
PfeI GAWTC 3 cut(s) 16, 400, 469
PkrI GCNGC 2 cut(s) 366, 490
PleI GAGTC 2 cut(s) 38, 70
PmaCI CACGTG 1 cut(s) 230
PmlI CACGTG 1 cut(s) 230
PpsI GAGTC 2 cut(s) 38, 70
Ppu21I YACGTR 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 549
Psp6I CCWGG 1 cut(s) 253
PspCI CACGTG 1 cut(s) 230
PspFI CCCAGC 2 cut(s) 71, 191
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 551
PspPI GGNCC 1 cut(s) 549
PspPPI RGGWCCY 1 cut(s) 549
PsuI RGATCY 2 cut(s) 266, 523
RsaI GTAC 1 cut(s) 508
RsaNI GTAC 1 cut(s) 507
SaqAI TTAA 2 cut(s) 372, 387
SatI GCNGC 2 cut(s) 365, 489
Sau3AI GATC 3 cut(s) 57, 266, 523
Sau96I GGNCC 1 cut(s) 549
SchI GAGTC 2 cut(s) 39, 70
ScrFI CCNGG 1 cut(s) 255
SetI ASST 8 cut(s) 175, 226, 232, 300, 314, 372, 393, 493
SinI GGWCC 1 cut(s) 549
Sse9I AATT 2 cut(s) 285, 452
SsiI CCGC 3 cut(s) 9, 101, 335
SspMI CTAG 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 155, 544
TaiI ACGT 1 cut(s) 232
TasI AATT 2 cut(s) 285, 452
TfiI GAWTC 3 cut(s) 16, 400, 469
Tru1I TTAA 2 cut(s) 372, 387
Tru9I TTAA 2 cut(s) 372, 387
TscAI CASTG 2 cut(s) 54, 160
TseI GCWGC 2 cut(s) 364, 488
TspDTI ATGAA 2 cut(s) 261, 606
TspRI CASTG 2 cut(s) 54, 160
VpaK11BI GGWCC 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 268
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.