Rw2G016440
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013008
Physical Location & Seq
Forward (+)
19476235 .. 19477091
857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G016440.1

Sequence Viewer

Length: 789 bp
ATGGGTGGTAGTTGTGGTTGGAGAAGTAGTAGTAGCAGTAATAGTTTCTGTCATGTCTTTCTTCTAGTTGTGACTCTGTTTTGCGGCGCGGTTTTCCGGCAGAGCCTATCAGATCCGAGCGACGAGGCATGTCTAACCCACCTGAGCCAATCCCTCCAAGACCCGAACCACTCGTTACAGAACTGGACCAAATCCACCTTCGCCAACCCATGTAGCGGCTTCACCTCTTATCTACAAGGCGCCACCTGCAACAATGGCCGAATCTACAAGCTCTCTCTCACCAACCTCTCTCTGCGCGGCTCCATCACACCTTTTCTCGCCAATTGCACCAATCTCCAGTCCCTCGATTTGTCTTCGAACTTCATCGCCGGCCCAATCCCGCCTGAGCTACAGTCCCTGGTCAACCTCGCCGTCCTTAACCTCTCGTCGAACAGGTTATCCGGCCCGATTCCGCCGCAGCTCACATTGTGTGCTTACCTCAACGTCATCGACCTCCACCAGAACCTTCTCACCGGCACGATTCCCCAGCAGCTCGGTCTGCTCGTGAGGCTCTCGGCGTTTGACGTCAGCAACAACAAGCTTTCGGGTCCGATTCCGAGTTCTCTTGGCAACAGGAGCGGGAGCCTGCCGAGATTCAATGCGACGTCGTTTGCCGGGAACAAGGACCTTTATGGTGGGTTAGCGAGCTTGGTGCTTAGCTTCACTGGGGTTTGTATATGGCTGAAAATCACAGAAAGGAAGATGGCTCTTGAAGAAGGCAAGATCACCCAGCTCATGCCTGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8

Protein Analysis

262

Amino Acids

28.19

Weight (kDa)

8.39

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013008)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49750 AT3G49750 AT5G65830
fragaria_vesca FvH4_1g18280
malus_domestica MD02G1190100.v1.1 MD15G1301200.v1.1
prunus_persica Prupe.6G216300_v2.0.a1
pyrus_communis pycom15g26380
rosa_chinensis RchiOBHm_Chr2g0108871
rosa_laevigata RLG00000017693
rosa_multiflora Rmu_sc0004830.1_g000001
rosa_roxburghii Rroxscaffold_2G00134760
rosa_rugosa Rorug02G0156500
rosa_samantha Rh2AG206900 Rh2BG218100 Rh2CG210100 Rh2DG213200
rosa_wichuraiana Rw2G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 254
AatII GACGTC 2 cut(s) 567, 647
Acc36I ACCTGC 1 cut(s) 254
AccB1I GGYRCC 1 cut(s) 239
AccBSI CCGCTC 1 cut(s) 618
AccII CGCG 2 cut(s) 89, 297
AciI CCGC 8 cut(s) 84, 89, 216, 297, 380, 452, 455, 618
AclWI GGATC 1 cut(s) 107
AcoI YGGCCR 1 cut(s) 256
AcyI GRCGYC 3 cut(s) 240, 564, 644
AdeI CACNNNGTG 1 cut(s) 468
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 2 cut(s) 637, 752
AjnI CCWGG 1 cut(s) 396
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AloI GAACNNNNNNTCC 2 cut(s) 422, 454
AluBI AGCT 8 cut(s) 271, 388, 460, 532, 580, 687, 699, 772
AluI AGCT 8 cut(s) 271, 388, 460, 532, 580, 687, 699, 772
AlwI GGATC 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 397
AoxI GGCC 3 cut(s) 256, 370, 442
ApeKI GCWGC 2 cut(s) 457, 529
AspLEI GCGC 3 cut(s) 89, 242, 297
AspS9I GGNCC 5 cut(s) 186, 371, 443, 587, 664
AsuC2I CCSGG 1 cut(s) 655
AsuHPI GGTGA 4 cut(s) 214, 271, 502, 757
AsuII TTCGAA 1 cut(s) 356
AvaII GGWCC 3 cut(s) 186, 587, 664
BanI GGYRCC 1 cut(s) 239
BauI CACGAG 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 469, 541
BccI CCATC 2 cut(s) 311, 736
BceAI ACGGC 1 cut(s) 395
BcgI CGANNNNNNTGC 2 cut(s) 673, 707
BciT130I CCWGG 1 cut(s) 398
BcnI CCSGG 1 cut(s) 655
BfaI CTAG 1 cut(s) 65
BfmI CTRYAG 1 cut(s) 389
BfoI RGCGCY 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 254
BisI GCNGC 6 cut(s) 85, 217, 298, 455, 458, 530
BlpI GCTNAGC 1 cut(s) 695
BlsI GCNGC 6 cut(s) 86, 218, 299, 456, 459, 531
Bme1390I CCNGG 2 cut(s) 398, 655
Bme18I GGWCC 3 cut(s) 186, 587, 664
BmgT120I GGNCC 5 cut(s) 186, 371, 443, 587, 664
BmiI GGNNCC 4 cut(s) 241, 301, 588, 623
BmrFI CCNGG 2 cut(s) 398, 655
BmrI ACTGGG 1 cut(s) 714
BmuI ACTGGG 1 cut(s) 714
BpiI GAAGAC 1 cut(s) 345
BpmI CTGGAG 1 cut(s) 320
Bpu10I CCTNAGC 2 cut(s) 143, 384
Bpu1102I GCTNAGC 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 655
BsaHI GRCGYC 3 cut(s) 240, 564, 644
BsaJI CCNNGG 1 cut(s) 396
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse118I RCCGGY 2 cut(s) 368, 512
Bse1I ACTGG 3 cut(s) 188, 337, 709
BseBI CCWGG 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMII CTCAG 2 cut(s) 134, 375
BseNI ACTGG 3 cut(s) 188, 337, 709
BseXI GCAGC 2 cut(s) 469, 541
BseYI CCCAGC 2 cut(s) 525, 768
Bsh1236I CGCG 2 cut(s) 89, 297
BshFI GGCC 3 cut(s) 258, 372, 444
BshNI GGYRCC 1 cut(s) 239
BsiSI CCGG 5 cut(s) 97, 369, 441, 513, 654
BslFI GGGAC 2 cut(s) 325, 379
BslI CCNNNNNNNGG 1 cut(s) 215
BsmFI GGGAC 2 cut(s) 325, 379
BsnI GGCC 3 cut(s) 258, 372, 444
Bsp119I TTCGAA 1 cut(s) 356
Bsp143I GATC 2 cut(s) 112, 762
Bsp1720I GCTNAGC 1 cut(s) 695
BspACI CCGC 8 cut(s) 84, 89, 216, 297, 380, 452, 455, 618
BspANI GGCC 3 cut(s) 258, 372, 444
BspCNI CTCAG 2 cut(s) 135, 376
BspFNI CGCG 2 cut(s) 89, 297
BspLI GGNNCC 4 cut(s) 241, 301, 588, 623
BspMI ACCTGC 1 cut(s) 254
BspPI GGATC 1 cut(s) 107
BspT104I TTCGAA 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 618
BsrFI RCCGGY 2 cut(s) 368, 512
BsrI ACTGG 3 cut(s) 188, 337, 709
BssAI RCCGGY 2 cut(s) 368, 512
BssECI CCNNGG 1 cut(s) 396
BssMI GATC 2 cut(s) 112, 762
BssNI GRCGYC 3 cut(s) 240, 564, 644
BssSI CACGAG 1 cut(s) 542
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 393
BstACI GRCGYC 3 cut(s) 240, 564, 644
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 3 cut(s) 370, 626, 685
BstDEI CTNAG 3 cut(s) 143, 384, 695
BstFNI CGCG 2 cut(s) 89, 297
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 3 cut(s) 89, 242, 297
BstKTI GATC 2 cut(s) 115, 765
BstMBI GATC 2 cut(s) 112, 762
BstMWI GCNNNNNNNGC 5 cut(s) 246, 255, 538, 547, 615
BstNI CCWGG 1 cut(s) 398
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 396, 653
BstSFI CTRYAG 1 cut(s) 389
BstUI CGCG 2 cut(s) 89, 297
BstV1I GCAGC 2 cut(s) 469, 541
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
BsuRI GGCC 3 cut(s) 258, 372, 444
BtgZI GCGATG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 702
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 3 cut(s) 370, 626, 685
CaiI CAGNNNCTG 1 cut(s) 397
CfoI GCGC 3 cut(s) 89, 242, 297
Cfr10I RCCGGY 2 cut(s) 368, 512
Cfr13I GGNCC 5 cut(s) 186, 371, 443, 587, 664
CviAII CATG 4 cut(s) 53, 129, 210, 775
DdeI CTNAG 3 cut(s) 143, 384, 695
DinI GGCGCC 1 cut(s) 241
DpnI GATC 2 cut(s) 114, 764
DpnII GATC 2 cut(s) 112, 762
DraIII CACNNNGTG 1 cut(s) 468
EaeI YGGCCR 1 cut(s) 256
EciI GGCGGA 1 cut(s) 441
Eco47I GGWCC 3 cut(s) 186, 587, 664
EcoO109I RGGNCCY 1 cut(s) 664
EcoRII CCWGG 1 cut(s) 396
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
FaeI CATG 4 cut(s) 56, 132, 213, 778
FaiI YATR 7 cut(s) 54, 130, 211, 672, 716, 718, 776
FaqI GGGAC 2 cut(s) 325, 379
FatI CATG 4 cut(s) 52, 128, 209, 774
FauI CCCGC 2 cut(s) 387, 611
Fnu4HI GCNGC 6 cut(s) 85, 217, 298, 455, 458, 530
Fsp4HI GCNGC 6 cut(s) 85, 217, 298, 455, 458, 530
FspBI CTAG 1 cut(s) 65
GlaI GCGC 3 cut(s) 88, 241, 296
GluI GCNGC 6 cut(s) 85, 217, 298, 455, 458, 530
GsaI CCCAGC 2 cut(s) 529, 772
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 3 cut(s) 258, 372, 444
HapII CCGG 5 cut(s) 97, 369, 441, 513, 654
HhaI GCGC 3 cut(s) 89, 242, 297
Hin1I GRCGYC 3 cut(s) 240, 564, 644
Hin1II CATG 4 cut(s) 56, 132, 213, 778
Hin6I GCGC 3 cut(s) 87, 240, 295
HinP1I GCGC 3 cut(s) 87, 240, 295
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 6 cut(s) 73, 261, 448, 520, 592, 633
HpaII CCGG 5 cut(s) 97, 369, 441, 513, 654
HphI GGTGA 4 cut(s) 214, 271, 502, 757
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 4 cut(s) 112, 117, 591, 597
Hpy188III TCNNGA 2 cut(s) 544, 749
Hpy8I GTNNAC 1 cut(s) 403
Hpy99I CGWCG 4 cut(s) 125, 430, 646, 649
HpyAV CCTTC 3 cut(s) 208, 515, 749
HpyCH4III ACNGT 1 cut(s) 393
HpyCH4IV ACGT 3 cut(s) 483, 564, 644
HpyCH4V TGCA 2 cut(s) 249, 327
HpyF10VI GCNNNNNNNGC 5 cut(s) 246, 255, 538, 547, 615
HpyF3I CTNAG 3 cut(s) 143, 384, 695
HpySE526I ACGT 3 cut(s) 483, 564, 644
Hsp92I GRCGYC 3 cut(s) 240, 564, 644
Hsp92II CATG 4 cut(s) 56, 132, 213, 778
HspAI GCGC 3 cut(s) 87, 240, 295
KasI GGCGCC 1 cut(s) 239
KroI GCCGGC 1 cut(s) 368
KroNI GCCGGC 1 cut(s) 370
Kzo9I GATC 2 cut(s) 112, 762
LmnI GCTCC 3 cut(s) 305, 615, 621
Lsp1109I GCAGC 2 cut(s) 469, 541
MaeI CTAG 1 cut(s) 65
MaeII ACGT 3 cut(s) 483, 564, 644
MaeIII GTNAC 2 cut(s) 70, 174
MalI GATC 2 cut(s) 114, 764
MbiI CCGCTC 1 cut(s) 618
MboI GATC 2 cut(s) 112, 762
MboII GAAGA 4 cut(s) 53, 345, 751, 764
MfeI CAATTG 1 cut(s) 322
MflI RGATCY 1 cut(s) 112
MluCI AATT 1 cut(s) 322
Mly113I GGCGCC 1 cut(s) 240
MlyI GAGTC 1 cut(s) 67
MroNI GCCGGC 1 cut(s) 368
MseI TTAA 1 cut(s) 417
MspI CCGG 5 cut(s) 97, 369, 441, 513, 654
MspR9I CCNGG 2 cut(s) 398, 655
MunI CAATTG 1 cut(s) 322
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 2 cut(s) 89, 297
MwoI GCNNNNNNNGC 5 cut(s) 246, 255, 538, 547, 615
NaeI GCCGGC 1 cut(s) 370
NarI GGCGCC 1 cut(s) 240
NciI CCSGG 1 cut(s) 655
NdeII GATC 2 cut(s) 112, 762
NgoMIV GCCGGC 1 cut(s) 368
NlaIII CATG 4 cut(s) 56, 132, 213, 778
NlaIV GGNNCC 4 cut(s) 241, 301, 588, 623
NmeAIII GCCGAG 2 cut(s) 533, 654
NmuCI GTSAC 1 cut(s) 70
NspI RCATGY 1 cut(s) 132
NspV TTCGAA 1 cut(s) 356
PaqCI CACCTGC 1 cut(s) 254
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PdiI GCCGGC 1 cut(s) 370
PfeI GAWTC 5 cut(s) 261, 448, 520, 592, 633
PkrI GCNGC 6 cut(s) 86, 218, 299, 456, 459, 531
PleI GAGTC 1 cut(s) 67
PluTI GGCGCC 1 cut(s) 243
PpsI GAGTC 1 cut(s) 67
PpuMI RGGWCCY 1 cut(s) 664
Psp5II RGGWCCY 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 396
PspFI CCCAGC 2 cut(s) 525, 768
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 4 cut(s) 241, 301, 588, 623
PspPI GGNCC 5 cut(s) 186, 371, 443, 587, 664
PspPPI RGGWCCY 1 cut(s) 664
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 1 cut(s) 112
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 6 cut(s) 85, 217, 298, 455, 458, 530
Sau3AI GATC 2 cut(s) 112, 762
Sau96I GGNCC 5 cut(s) 186, 371, 443, 587, 664
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 2 cut(s) 398, 655
SfcI CTRYAG 1 cut(s) 389
SfoI GGCGCC 1 cut(s) 241
SfuI TTCGAA 1 cut(s) 356
SinI GGWCC 3 cut(s) 186, 587, 664
Sse9I AATT 1 cut(s) 322
SsiI CCGC 8 cut(s) 84, 89, 216, 297, 380, 452, 455, 618
SspDI GGCGCC 1 cut(s) 239
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 396, 653
TaaI ACNGT 1 cut(s) 393
TaiI ACGT 3 cut(s) 486, 567, 647
TaqI TCGA 4 cut(s) 345, 356, 428, 489
TaqII GACCGA 1 cut(s) 524
TasI AATT 1 cut(s) 322
TauI GCSGC 4 cut(s) 87, 219, 300, 457
TfiI GAWTC 5 cut(s) 261, 448, 520, 592, 633
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 1 cut(s) 709
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 2 cut(s) 457, 529
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 1 cut(s) 352
TspRI CASTG 1 cut(s) 709
VpaK11BI GGWCC 3 cut(s) 186, 587, 664
XceI RCATGY 1 cut(s) 132
XspI CTAG 1 cut(s) 65
ZraI GACGTC 2 cut(s) 565, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.