Rw4G030400

Lysine-rich arabinogalactan protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015270
Physical Location & Seq
Reverse (-)
57126059 .. 57127011
953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030400.1

Sequence Viewer

Length: 792 bp
ATGGCTTCGACTCTGATGGCCCTGGTTTTGACCTGTCTCACCTTTCAACTAGTCCTTACCAATGCACAAACACCAGCAGCTGCGCCCGCTACGGCTACAACGCCGCCGCCACCTACCACACCGGTTGCACCAGCTGCGCAACCACCGACTGCTGTAACACCACCTCCTGTAACTGCAGCAGCAACCCCACCCACAACCCCGCCAACAACATCACCACCCCCTAAAGTTGCACCATCCACAAGCCCGACAGTCCCACCCCCAAAAACTCCACCAACACAACCACCAAAGATTTCACCTGTCTCAACTCCATCTCAGCCACCAACACTGCCACCACCACCACCACCAGCTGTTTCACCACCACTACCACCTCCACAAGTAGCACCACAGGTATCCCCAACCCCAGCTCCTGTTAAGAAATCGCCGGCGCCAGCACCAGCCAAGGCAGCACCAGTGCCCTCACCATCACAAGCACCGCCAGTACCAGCGCCAACACCAGTTGTCGCGGAACCAGCTCCAGCCCCTGTGGAAGTGCCGTCACCTGCACCTGCTCCACATAAACACAAGAAAAGGAAGCACAAGCACAGGAGGCATCATCATGCACCAGCACCGGCGCCAACTGTCCAAAGTCCCCCAGCCCCACCTATAGTGACAGATACAGAGGATACAACACCAGCACCATCACCAACTTTGGATTTGAGCGGAGGAAATGCACTGCACCAGAGAGGAGCAAAGTCTGCTATTTGGATTACGACTGGATTAGCTATTGCTATACTGCTGGCAATGACAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020 GO:0031224 GO:0031225 GO:0044425
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

26.3

Weight (kDa)

10.3

Isoelectric Point (pI)

99.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015270)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68725
fragaria_vesca FvH4_4g28430
malus_domestica MD13G1050000.v1.1
prunus_persica Prupe.1G301700_v2.0.a1
pyrus_communis pycom13g04430 pycom16g04470
rosa_chinensis RchiOBHm_Chr4g0436381
rosa_laevigata RLG00000006523
rosa_multiflora Rmu_sc0007205.1_g000001
rosa_roxburghii Rroxscaffold_5G00377550
rosa_rugosa Rorug04G0293400
rosa_samantha Rh4AG347700 Rh4BG356500 Rh4CG371100 Rh4DG350200
rosa_wichuraiana Rw4G030400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 547, 553
Acc16I TGCGCA 1 cut(s) 138
Acc36I ACCTGC 2 cut(s) 547, 553
AccB1I GGYRCC 2 cut(s) 424, 610
AccBSI CCGCTC 1 cut(s) 699
AccII CGCG 1 cut(s) 503
AciI CCGC 7 cut(s) 87, 104, 107, 200, 473, 503, 699
AcyI GRCGYC 2 cut(s) 425, 611
AfaI GTAC 1 cut(s) 480
AgeI ACCGGT 1 cut(s) 121
AgsI TTSAA 1 cut(s) 47
AhlI ACTAGT 1 cut(s) 49
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 7 cut(s) 80, 134, 347, 404, 512, 761, 789
AluI AGCT 7 cut(s) 80, 134, 347, 404, 512, 761, 789
Alw26I GTCTC 2 cut(s) 41, 304
AlwNI CAGNNNCTG 3 cut(s) 80, 407, 521
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 6 cut(s) 77, 80, 134, 176, 179, 443
AsiGI ACCGGT 1 cut(s) 121
AspLEI GCGC 5 cut(s) 85, 139, 427, 487, 613
AspS9I GGNCC 1 cut(s) 19
AsuHPI GGTGA 7 cut(s) 31, 204, 285, 345, 450, 528, 672
BaeGI GKGCMC 1 cut(s) 456
BanI GGYRCC 2 cut(s) 424, 610
BbvI GCAGC 6 cut(s) 67, 89, 121, 188, 191, 455
BccI CCATC 5 cut(s) 10, 241, 316, 469, 685
BceAI ACGGC 2 cut(s) 108, 517
BciT130I CCWGG 1 cut(s) 23
BciVI GTATCC 2 cut(s) 400, 655
BcoDI GTCTC 2 cut(s) 41, 304
BcuI ACTAGT 1 cut(s) 49
BfaI CTAG 2 cut(s) 50, 790
BfmI CTRYAG 2 cut(s) 174, 642
BfoI RGCGCY 3 cut(s) 428, 488, 614
BfuAI ACCTGC 2 cut(s) 547, 553
BfuI GTATCC 2 cut(s) 400, 655
BisI GCNGC 8 cut(s) 78, 81, 104, 107, 135, 177, 180, 444
BlsI GCNGC 8 cut(s) 79, 82, 105, 108, 136, 178, 181, 445
Bme1390I CCNGG 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 19
BmiI GGNNCC 3 cut(s) 426, 507, 612
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 1 cut(s) 598
BpmI CTGGAG 1 cut(s) 498
BsaHI GRCGYC 2 cut(s) 425, 611
BsaJI CCNNGG 2 cut(s) 21, 438
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 4 cut(s) 148, 178, 388, 418
Bse118I RCCGGY 3 cut(s) 121, 421, 607
Bse1I ACTGG 4 cut(s) 449, 476, 494, 757
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 21, 438
BseGI GGATG 1 cut(s) 233
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 1 cut(s) 326
BseNI ACTGG 4 cut(s) 449, 476, 494, 757
BseRI GAGGAG 1 cut(s) 738
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 6 cut(s) 67, 89, 121, 188, 191, 455
BseYI CCCAGC 2 cut(s) 400, 631
BsgI GTGCAG 2 cut(s) 525, 698
Bsh1236I CGCG 1 cut(s) 503
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 2 cut(s) 424, 610
BshTI ACCGGT 1 cut(s) 121
BsiSI CCGG 3 cut(s) 122, 422, 608
BslFI GGGAC 2 cut(s) 236, 612
BsmAI GTCTC 2 cut(s) 41, 304
BsmFI GGGAC 2 cut(s) 236, 612
BsnI GGCC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 456
BspACI CCGC 7 cut(s) 87, 104, 107, 200, 473, 503, 699
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 1 cut(s) 503
BspLI GGNNCC 3 cut(s) 426, 507, 612
BspMAI CTGCAG 1 cut(s) 178
BspMI ACCTGC 2 cut(s) 547, 553
BspT107I GGYRCC 2 cut(s) 424, 610
BsrBI CCGCTC 1 cut(s) 699
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 3 cut(s) 121, 421, 607
BsrI ACTGG 4 cut(s) 449, 476, 494, 757
BssAI RCCGGY 3 cut(s) 121, 421, 607
BssECI CCNNGG 2 cut(s) 21, 438
BssNI GRCGYC 2 cut(s) 425, 611
BssT1I CCWWGG 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 250, 619
BstACI GRCGYC 2 cut(s) 425, 611
BstAPI GCANNNNNTGC 2 cut(s) 134, 734
BstC8I GCNNGC 4 cut(s) 87, 423, 429, 777
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 233
BstFNI CGCG 1 cut(s) 503
BstH2I RGCGCY 3 cut(s) 428, 488, 614
BstHHI GCGC 5 cut(s) 85, 139, 427, 487, 613
BstMAI GTCTC 2 cut(s) 41, 304
BstMWI GCNNNNNNNGC 6 cut(s) 86, 134, 443, 509, 586, 734
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 2 cut(s) 174, 642
BstSLI GKGCMC 1 cut(s) 456
BstUI CGCG 1 cut(s) 503
BstV1I GCAGC 6 cut(s) 67, 89, 121, 188, 191, 455
BsuI GTATCC 2 cut(s) 400, 655
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 1 cut(s) 233
BtsI GCAGTG 2 cut(s) 323, 710
BtsIMutI CAGTG 3 cut(s) 323, 456, 710
BveI ACCTGC 2 cut(s) 547, 553
Cac8I GCNNGC 4 cut(s) 87, 423, 429, 777
CaiI CAGNNNCTG 3 cut(s) 80, 407, 521
CfoI GCGC 5 cut(s) 85, 139, 427, 487, 613
Cfr10I RCCGGY 3 cut(s) 121, 421, 607
Cfr13I GGNCC 1 cut(s) 19
Csp6I GTAC 1 cut(s) 479
CspAI ACCGGT 1 cut(s) 121
CspCI CAANNNNNGTGG 2 cut(s) 106, 141
CviAII CATG 1 cut(s) 596
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 1 cut(s) 312
DinI GGCGCC 2 cut(s) 426, 612
Eco130I CCWWGG 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 438
EgeI GGCGCC 2 cut(s) 426, 612
EheI GGCGCC 2 cut(s) 426, 612
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 1 cut(s) 599
FaiI YATR 4 cut(s) 555, 597, 644, 770
FaqI GGGAC 2 cut(s) 236, 612
FatI CATG 1 cut(s) 595
FauI CCCGC 2 cut(s) 94, 207
Fnu4HI GCNGC 8 cut(s) 78, 81, 104, 107, 135, 177, 180, 444
FokI GGATG 1 cut(s) 220
Fsp4HI GCNGC 8 cut(s) 78, 81, 104, 107, 135, 177, 180, 444
FspBI CTAG 2 cut(s) 50, 790
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 5 cut(s) 84, 138, 426, 486, 612
GluI GCNGC 8 cut(s) 78, 81, 104, 107, 135, 177, 180, 444
GsaI CCCAGC 2 cut(s) 404, 635
GsuI CTGGAG 1 cut(s) 498
HaeII RGCGCY 3 cut(s) 428, 488, 614
HaeIII GGCC 1 cut(s) 20
HapII CCGG 3 cut(s) 122, 422, 608
HhaI GCGC 5 cut(s) 85, 139, 427, 487, 613
Hin1I GRCGYC 2 cut(s) 425, 611
Hin1II CATG 1 cut(s) 599
Hin6I GCGC 5 cut(s) 83, 137, 425, 485, 611
HinP1I GCGC 5 cut(s) 83, 137, 425, 485, 611
HinfI GANTC 1 cut(s) 10
HpaII CCGG 3 cut(s) 122, 422, 608
HphI GGTGA 7 cut(s) 31, 204, 285, 345, 450, 528, 672
Hpy188I TCNGA 1 cut(s) 15
HpyCH4III ACNGT 2 cut(s) 250, 619
HpyCH4V TGCA 8 cut(s) 65, 128, 176, 230, 542, 599, 710, 715
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 134, 443, 509, 586, 734
HpyF3I CTNAG 1 cut(s) 312
Hsp92I GRCGYC 2 cut(s) 425, 611
Hsp92II CATG 1 cut(s) 599
HspAI GCGC 5 cut(s) 83, 137, 425, 485, 611
KasI GGCGCC 2 cut(s) 424, 610
KroI GCCGGC 1 cut(s) 421
KroNI GCCGGC 1 cut(s) 423
LmnI GCTCC 4 cut(s) 409, 517, 553, 725
Lsp1109I GCAGC 6 cut(s) 67, 89, 121, 188, 191, 455
LweI GCATC 1 cut(s) 598
MaeI CTAG 2 cut(s) 50, 790
MaeIII GTNAC 4 cut(s) 154, 169, 534, 646
MbiI CCGCTC 1 cut(s) 699
MhlI GDGCHC 1 cut(s) 456
Mly113I GGCGCC 2 cut(s) 425, 611
MlyI GAGTC 1 cut(s) 4
MnlI CCTC 7 cut(s) 174, 378, 466, 579, 652, 695, 716
MreI CGCCGGCG 1 cut(s) 421
MroNI GCCGGC 1 cut(s) 421
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 594
MspA1I CMGCKG 3 cut(s) 80, 134, 347
MspI CCGG 3 cut(s) 122, 422, 608
MspR9I CCNGG 1 cut(s) 23
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 1 cut(s) 503
MwoI GCNNNNNNNGC 6 cut(s) 86, 134, 443, 509, 586, 734
NaeI GCCGGC 1 cut(s) 423
NarI GGCGCC 2 cut(s) 425, 611
NgoMIV GCCGGC 1 cut(s) 421
NlaIII CATG 1 cut(s) 599
NlaIV GGNNCC 3 cut(s) 426, 507, 612
NmuCI GTSAC 2 cut(s) 534, 646
NsbI TGCGCA 1 cut(s) 138
PaqCI CACCTGC 2 cut(s) 547, 553
PdiI GCCGGC 1 cut(s) 423
PinAI ACCGGT 1 cut(s) 121
PkrI GCNGC 8 cut(s) 79, 82, 105, 108, 136, 178, 181, 445
PleI GAGTC 1 cut(s) 4
PluTI GGCGCC 2 cut(s) 428, 614
PpsI GAGTC 1 cut(s) 4
Psp6I CCWGG 1 cut(s) 21
PspFI CCCAGC 2 cut(s) 400, 631
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 426, 507, 612
PspPI GGNCC 1 cut(s) 19
PstI CTGCAG 1 cut(s) 178
PstNI CAGNNNCTG 3 cut(s) 80, 407, 521
PvuII CAGCTG 3 cut(s) 80, 134, 347
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 594
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 8 cut(s) 78, 81, 104, 107, 135, 177, 180, 444
Sau96I GGNCC 1 cut(s) 19
SchI GAGTC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 1 cut(s) 456
SfaNI GCATC 1 cut(s) 598
SfcI CTRYAG 2 cut(s) 174, 642
SfoI GGCGCC 2 cut(s) 426, 612
SgrAI CRCCGGYG 2 cut(s) 421, 607
SmiMI CAYNNNNRTG 1 cut(s) 594
SpeI ACTAGT 1 cut(s) 49
SsiI CCGC 7 cut(s) 87, 104, 107, 200, 473, 503, 699
SspDI GGCGCC 2 cut(s) 424, 610
SspMI CTAG 2 cut(s) 50, 790
StyD4I CCNGG 1 cut(s) 21
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 250, 619
TaqI TCGA 1 cut(s) 8
TauI GCSGC 2 cut(s) 106, 109
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 3 cut(s) 330, 456, 717
TseFI GTSAC 2 cut(s) 534, 646
TseI GCWGC 6 cut(s) 77, 80, 134, 176, 179, 443
Tsp45I GTSAC 2 cut(s) 534, 646
TspRI CASTG 3 cut(s) 330, 456, 717
XspI CTAG 2 cut(s) 50, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.