AT1G01460

phosphatidylinositol 4-phosphate 5-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006578
Physical Location & Seq
Forward (+)
168559 .. 171386
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G01460.1

Sequence Viewer

Length: 1284 bp
ATGTTTACGCGGGAGATAACAGCAAAGGACGTGAAAGCCACTGAGAAAAACAGGATTCGATACTCTTCCAAACACATAAAACATCTTCCTCCTGGAACCATTACTGAGTTCGAATGGAAGGATTATTGTCCCTTGGGATTCAGACTTATACAGGAACTTGAGGATATCAATCATGATGAGTACATGAAGTCTATCTGTAATGATGAGACCTTAAGGAAGCTTTCTACTAGCAAAGTTGGTAACATGTTTCTTCTTTCCAAAGATGACCGTTTTCTCATTAAGATTCTACGCAAATCCGAAATCAAGGTTATACTAGAGATGTTGCCTGGTTACTTCCGTCATATCCATAAATACAGGTCAACCTTGTTGTCCAAGAATTATGGAGCTCACTCTGTGAAGCCTATTGGAGGTGTTAAGACATATTTTGTCGTCATGTCAAACATACTGCAGTCGGATGTTTTTATGAACAAGGTCTATGATCTAAAGGGTTCATCTCAAGGTCGGACTAACAAGAAGATCAAAGTGAGAGATAAAACCATATTGAAGGATATCGACCTTGATTTTTGTTTCTATGTGGATTCTTTGGCCAGACACCGTCTCATTAAGCAAACGAAGCTAGATTGTGAACTTTTGGAAGATGAAGGCATCATGGATTACAGCTTAATGCTTGGTTTACAAGTGAAAGGCTCGTGTCATGGTTCAATTGATGAATTAATCCCGGTATACGACAGCTTCACATCACGAGGCTCTGTTGACAGCAATAGCTCTAAGTTTATGAAGACAGCTTCAAACTCTCCAGATAGATCATCTTCTACAATGTATTCATGCACTCCCAGCAGAAATTCAGTTGATAGTGAGAACTCAGTGAATATACAATCAGTAGCAAGTATAAGCCCTAGTCCAGCACAAACCAACGCTTCTGATTCTCCATATGAAAGCCTTGTTTCCAAAACAAATCTCACCAACATCTTCCAAAACAGCTCAAGCACTAACTTCGGTATGAAGATACCAGGACGAGCACGACGGGTTGGGAGAGGAGAATCAGGGAGTGTGGTGGGAAAACAGAGCAGAGAAGGAGGAGAAGAATGGTACGATGTGATTTTGTATCTTGGGATTATAGACATTTTCCAAGACTACGGTGTGAGAAAACGCCTTGAACATTGTTACAAGTCAATTCAACACAGCTCTAAGACAATCTCAGCCGTCCATCCCAAGATGTATTCTTCTCGTTTCCAAGACTTTGTCTCTCAGATCTTCTTACCAGACGAAGATCCTTCTCACTGA

Protein Analysis

427

Amino Acids

48.55

Weight (kDa)

8.97

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 66 - 418 2.2e-77 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 723
AccII CGCG 1 cut(s) 10
AciI CCGC 1 cut(s) 10
AclWI GGATC 1 cut(s) 1265
AcoI YGGCCR 1 cut(s) 585
AcsI RAATTY 1 cut(s) 841
AdeI CACNNNGTG 1 cut(s) 394
AfaI GTAC 2 cut(s) 182, 1091
AfiI CCNNNNNNNGG 1 cut(s) 407
AflII CTTAAG 1 cut(s) 211
AflIII ACRYGT 1 cut(s) 243
AgsI TTSAA 5 cut(s) 544, 702, 789, 1157, 1178
AjiI CACGTC 1 cut(s) 31
AjnI CCWGG 3 cut(s) 91, 325, 1009
AluBI AGCT 9 cut(s) 220, 386, 616, 660, 732, 765, 785, 981, 1185
AluI AGCT 9 cut(s) 220, 386, 616, 660, 732, 765, 785, 981, 1185
Alw21I GWGCWC 2 cut(s) 388, 1021
Alw26I GTCTC 3 cut(s) 200, 602, 1249
AlwI GGATC 1 cut(s) 1265
AoxI GGCC 1 cut(s) 585
ApoI RAATTY 1 cut(s) 841
AseI ATTAAT 1 cut(s) 713
AsuC2I CCSGG 1 cut(s) 719
AsuHPI GGTGA 1 cut(s) 952
AsuII TTCGAA 1 cut(s) 111
BalI TGGCCA 1 cut(s) 587
BanII GRGCYC 1 cut(s) 388
BauI CACGAG 2 cut(s) 688, 741
BbsI GAAGAC 1 cut(s) 785
Bbv12I GWGCWC 2 cut(s) 388, 1021
BccI CCATC 1 cut(s) 1215
BceAI ACGGC 1 cut(s) 1187
BcgI CGANNNNNNTGC 2 cut(s) 976, 1010
BciT130I CCWGG 3 cut(s) 93, 327, 1011
BcnI CCSGG 1 cut(s) 719
BcoDI GTCTC 3 cut(s) 200, 602, 1249
BfaI CTAG 4 cut(s) 228, 314, 617, 897
BfmI CTRYAG 1 cut(s) 446
BfrI CTTAAG 1 cut(s) 211
BglII AGATCT 1 cut(s) 1251
Bme1390I CCNGG 4 cut(s) 93, 327, 719, 1011
BmgBI CACGTC 1 cut(s) 31
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 4 cut(s) 93, 327, 719, 1011
BmsI GCATC 1 cut(s) 654
BpiI GAAGAC 1 cut(s) 785
BpmI CTGGAG 1 cut(s) 780
Bpu14I TTCGAA 1 cut(s) 111
BpuEI CTTGAG 3 cut(s) 179, 480, 967
BpuMI CCSGG 1 cut(s) 719
BsaBI GATNNNNATC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 3 cut(s) 93, 327, 1011
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 2 cut(s) 460, 1207
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMII CTCAG 5 cut(s) 33, 96, 876, 1212, 1262
BseRI GAGGAG 2 cut(s) 1050, 1092
BseYI CCCAGC 1 cut(s) 833
Bsh1236I CGCG 1 cut(s) 10
BshFI GGCC 1 cut(s) 587
BsiHKAI GWGCWC 2 cut(s) 388, 1021
BsiSI CCGG 1 cut(s) 719
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 407
BsmAI GTCTC 3 cut(s) 200, 602, 1249
BsmBI CGTCTC 1 cut(s) 602
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 1 cut(s) 587
Bso31I GGTCTC 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 388, 1021
Bsp143I GATC 5 cut(s) 478, 516, 803, 1251, 1270
BspACI CCGC 1 cut(s) 10
BspANI GGCC 1 cut(s) 587
BspCNI CTCAG 5 cut(s) 34, 97, 875, 1211, 1261
BspFNI CGCG 1 cut(s) 10
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 1 cut(s) 97
BspMAI CTGCAG 1 cut(s) 450
BspPI GGATC 1 cut(s) 1265
BspT104I TTCGAA 1 cut(s) 111
BspTI CTTAAG 1 cut(s) 211
BspTNI GGTCTC 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 5 cut(s) 478, 516, 803, 1251, 1270
BssNAI GTATAC 1 cut(s) 724
BssSI CACGAG 2 cut(s) 688, 741
BssT1I CCWWGG 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 724
Bst2BI CACGAG 2 cut(s) 688, 741
Bst2UI CCWGG 3 cut(s) 93, 327, 1011
Bst4CI ACNGT 3 cut(s) 269, 596, 1139
Bst6I CTCTTC 1 cut(s) 70
BstAFI CTTAAG 1 cut(s) 211
BstBI TTCGAA 1 cut(s) 111
BstDEI CTNAG 7 cut(s) 42, 105, 768, 862, 1188, 1198, 1248
BstENI CCTNNNNNAGG 1 cut(s) 405
BstF5I GGATG 2 cut(s) 460, 1207
BstFNI CGCG 1 cut(s) 10
BstKTI GATC 5 cut(s) 481, 519, 806, 1254, 1273
BstMAI GTCTC 3 cut(s) 200, 602, 1249
BstMBI GATC 5 cut(s) 478, 516, 803, 1251, 1270
BstMWI GCNNNNNNNGC 2 cut(s) 613, 834
BstNI CCWGG 3 cut(s) 93, 327, 1011
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 4 cut(s) 91, 325, 717, 1009
BstSFI CTRYAG 1 cut(s) 446
BstUI CGCG 1 cut(s) 10
BstV2I GAAGAC 1 cut(s) 785
BstX2I RGATCY 2 cut(s) 1251, 1270
BstYI RGATCY 2 cut(s) 1251, 1270
BstZ17I GTATAC 1 cut(s) 724
BsuRI GGCC 1 cut(s) 587
BtrI CACGTC 1 cut(s) 31
BtsCI GGATG 2 cut(s) 460, 1207
BtsIMutI CAGTG 3 cut(s) 39, 870, 1279
CciI TCATGA 1 cut(s) 172
Csp6I GTAC 2 cut(s) 181, 1090
CviAII CATG 7 cut(s) 173, 184, 244, 433, 649, 695, 825
CviQI GTAC 2 cut(s) 181, 1090
DdeI CTNAG 7 cut(s) 42, 105, 768, 862, 1188, 1198, 1248
DpnI GATC 5 cut(s) 480, 518, 805, 1253, 1272
DpnII GATC 5 cut(s) 478, 516, 803, 1251, 1270
DraIII CACNNNGTG 1 cut(s) 394
EaeI YGGCCR 1 cut(s) 585
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 386
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 200
Eco32I GATATC 2 cut(s) 166, 550
Eco53kI GAGCTC 1 cut(s) 386
EcoICRI GAGCTC 1 cut(s) 386
EcoNI CCTNNNNNAGG 1 cut(s) 405
EcoRII CCWGG 3 cut(s) 91, 325, 1009
EcoRV GATATC 2 cut(s) 166, 550
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 602
FaeI CATG 7 cut(s) 176, 187, 247, 436, 652, 698, 828
FaqI GGGAC 1 cut(s) 114
FatI CATG 7 cut(s) 172, 183, 243, 432, 648, 694, 824
FauI CCCGC 1 cut(s) 3
FauNDI CATATG 1 cut(s) 931
FblI GTMKAC 1 cut(s) 723
FokI GGATG 2 cut(s) 467, 1194
FriOI GRGCYC 1 cut(s) 388
FspBI CTAG 4 cut(s) 228, 314, 617, 897
GsaI CCCAGC 1 cut(s) 837
GsuI CTGGAG 1 cut(s) 780
HaeIII GGCC 1 cut(s) 587
HapII CCGG 1 cut(s) 719
Hin1II CATG 7 cut(s) 176, 187, 247, 436, 652, 698, 828
HincII GTYRAC 2 cut(s) 360, 754
HindII GTYRAC 2 cut(s) 360, 754
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 6 cut(s) 55, 138, 283, 578, 923, 1040
HpaII CCGG 1 cut(s) 719
HphI GGTGA 1 cut(s) 952
Hpy166II GTNNAC 6 cut(s) 6, 360, 626, 674, 724, 754
Hpy188I TCNGA 6 cut(s) 143, 298, 454, 504, 922, 1251
Hpy188III TCNNGA 3 cut(s) 173, 741, 797
Hpy8I GTNNAC 6 cut(s) 6, 360, 626, 674, 724, 754
Hpy99I CGWCG 1 cut(s) 1026
HpyAV CCTTC 5 cut(s) 112, 538, 635, 1067, 1284
HpyCH4III ACNGT 3 cut(s) 269, 596, 1139
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 2 cut(s) 448, 828
HpyF10VI GCNNNNNNNGC 2 cut(s) 613, 834
HpyF3I CTNAG 7 cut(s) 42, 105, 768, 862, 1188, 1198, 1248
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 7 cut(s) 176, 187, 247, 436, 652, 698, 828
Kzo9I GATC 5 cut(s) 478, 516, 803, 1251, 1270
LmnI GCTCC 1 cut(s) 383
LweI GCATC 1 cut(s) 654
MaeI CTAG 4 cut(s) 228, 314, 617, 897
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 3 cut(s) 239, 329, 1163
MalI GATC 5 cut(s) 480, 518, 805, 1253, 1272
MboI GATC 5 cut(s) 478, 516, 803, 1251, 1270
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 2 cut(s) 1251, 1270
MhlI GDGCHC 2 cut(s) 388, 1021
MlsI TGGCCA 1 cut(s) 587
MluCI AATT 5 cut(s) 376, 702, 710, 841, 1173
MluNI TGGCCA 1 cut(s) 587
MmeI TCCRAC 2 cut(s) 432, 482
MnlI CCTC 6 cut(s) 99, 154, 401, 737, 1028, 1070
Mox20I TGGCCA 1 cut(s) 587
MscI TGGCCA 1 cut(s) 587
MseI TTAA 6 cut(s) 212, 279, 414, 603, 662, 713
Msp20I TGGCCA 1 cut(s) 587
MspCI CTTAAG 1 cut(s) 211
MspI CCGG 1 cut(s) 719
MspR9I CCNGG 4 cut(s) 93, 327, 719, 1011
MunI CAATTG 1 cut(s) 702
MvaI CCWGG 3 cut(s) 93, 327, 1011
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 2 cut(s) 613, 834
NciI CCSGG 1 cut(s) 719
NdeI CATATG 1 cut(s) 931
NdeII GATC 5 cut(s) 478, 516, 803, 1251, 1270
NlaIII CATG 7 cut(s) 176, 187, 247, 436, 652, 698, 828
NlaIV GGNNCC 1 cut(s) 97
NspI RCATGY 1 cut(s) 247
NspV TTCGAA 1 cut(s) 111
PagI TCATGA 1 cut(s) 172
PciI ACATGT 1 cut(s) 243
PfeI GAWTC 6 cut(s) 55, 138, 283, 578, 923, 1040
PflFI GACNNNGTC 2 cut(s) 594, 1241
PfoI TCCNGGA 1 cut(s) 91
PscI ACATGT 1 cut(s) 243
PshBI ATTAAT 1 cut(s) 713
Psp124BI GAGCTC 1 cut(s) 388
Psp6I CCWGG 3 cut(s) 91, 325, 1009
PspFI CCCAGC 1 cut(s) 833
PspGI CCWGG 3 cut(s) 91, 325, 1009
PspN4I GGNNCC 1 cut(s) 97
PstI CTGCAG 1 cut(s) 450
PsuI RGATCY 2 cut(s) 1251, 1270
PsyI GACNNNGTC 2 cut(s) 594, 1241
RsaI GTAC 2 cut(s) 182, 1091
RsaNI GTAC 2 cut(s) 181, 1090
SacI GAGCTC 1 cut(s) 388
SaqAI TTAA 6 cut(s) 212, 279, 414, 603, 662, 713
Sau3AI GATC 5 cut(s) 478, 516, 803, 1251, 1270
ScrFI CCNGG 4 cut(s) 93, 327, 719, 1011
SduI GDGCHC 2 cut(s) 388, 1021
SfaNI GCATC 1 cut(s) 654
SfcI CTRYAG 1 cut(s) 446
SfuI TTCGAA 1 cut(s) 111
SmlI CTYRAG 4 cut(s) 158, 211, 495, 982
SmoI CTYRAG 4 cut(s) 158, 211, 495, 982
Sse9I AATT 5 cut(s) 376, 702, 710, 841, 1173
SsiI CCGC 1 cut(s) 10
SspMI CTAG 4 cut(s) 228, 314, 617, 897
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 4 cut(s) 91, 325, 717, 1009
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 3 cut(s) 269, 596, 1139
TaiI ACGT 1 cut(s) 33
TaqI TCGA 3 cut(s) 58, 111, 552
TasI AATT 5 cut(s) 376, 702, 710, 841, 1173
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 6 cut(s) 55, 138, 283, 578, 923, 1040
Tru1I TTAA 6 cut(s) 212, 279, 414, 603, 662, 713
Tru9I TTAA 6 cut(s) 212, 279, 414, 603, 662, 713
TscAI CASTG 2 cut(s) 46, 870
TspDTI ATGAA 9 cut(s) 200, 479, 480, 654, 723, 791, 813, 948, 1016
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 2 cut(s) 46, 870
Tth111I GACNNNGTC 2 cut(s) 594, 1241
Vha464I CTTAAG 1 cut(s) 211
VspI ATTAAT 1 cut(s) 713
XagI CCTNNNNNAGG 1 cut(s) 405
XapI RAATTY 1 cut(s) 841
XceI RCATGY 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 723
XspI CTAG 4 cut(s) 228, 314, 617, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.