RLG00000027957

phosphatidylinositol 4-phosphate 5-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006578
Physical Location & Seq
Reverse (-)
17151930 .. 17156314
4385 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027957

Sequence Viewer

Length: 1215 bp
ATGGAGATTCTGAAGGAGGAGCTTTCACCGGCTGACTTTAAGGCTACTAAAAGTAACCGAATCAGATATTCTAGAAATGACTTTAGACAGCTTCCTTATTTCGCAACTGACTTTGAATGGAAAGATTATAGCCCTGCAGTTTTCAGACGTATACTGGAGCTTGACAGTATTGAATATTCCGATTACCAGTTGTCCGTATGTGGAGATGAGACTCTAAAGGAGGTTACGCTTTCGGGGAAACTTGGTATGGTGTTTTTATTGTCCAACGACAAACGGTTTATTCTCAAAACCCTGCGCAAATCTGAGGTGAAGATCTACCTAGAGATGCTTCCCAACTACTACGACCATCTTATGAAGTATGGATCCTCACTACTAGCCAAACTGTATGGACTTCATGTTGTCAGGCCAGCAGGAGGTGTTAAGGTTTACTTTGTTGTTTTTGGAAACGTAATCCCGTCAGATATTTGCATATTTGAGCATTATGACCTCAAAGGCTCTTCAAAAGGTCGAGCAGAGAACAAAGTCATTATTCAGGAGAGACCCGTACATAAGGATATGGATTTTGATTTTCGCTTTTACCTGGATCCATTGGTTCGAGATCGGCTCTTAGCGCAAATCAAGACTGACTGCGAGTTTTTGGAATCTGAAGGCGTCATGGATTATAGTTTGTTTCTTGCTATACAAATAGAATCACCACATCAAGTTTCAGTTGATAAAAGAACTTCCAGAAGGACTGATAAAGAGATAATAGACGATACTTCAGAAGGAAGTGATGAAAGCTCAGAGTTAACTCTTGCTGATATTTGCGACCTACTTGACAGGCCTGGTTTTAGATTTGGTGCAAGCCTGCCAGCACGTGCTGTCCGGGCCTTCAAGAAAGAAATAGGAAGTTCGAGATCAAATAGGTCCAGAAACTCAAGAGCACAGTCATCAGCGCAGGAATGCTTCAATGTTCTTTTATTCTTTGGAATAGTAGATATTTGTCAGAATTACAATATGAGAAAACGCCTTGAGCATGCTTGCAAGTCCATTAAATATGACTCAAAGTCCATTGCAACTGTGAACCCCAAAGCTTATTCTTCTCGCTTTCAAGAGTTCATGAGCGAAGTGTTTCTACCAGAAGTGTCGGACGACATTTCTGATAGCCGGGTATCAGGCGCATCATCATCTAACAGGGTCTCAGCCGCATCATCATCGAAGATTTCTGTTAGATGA

Protein Analysis

405

Amino Acids

46.19

Weight (kDa)

7.55

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 67 - 369 1.3e-78 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 296
AccI GTMKAC 1 cut(s) 151
AciI CCGC 1 cut(s) 1185
AclWI GGATC 4 cut(s) 357, 370, 578, 591
AcuI CTGAAG 3 cut(s) 32, 666, 744
AcvI CACGTG 1 cut(s) 857
AcyI GRCGYC 1 cut(s) 651
AfaI GTAC 1 cut(s) 546
AfiI CCNNNNNNNGG 1 cut(s) 413
AgsI TTSAA 6 cut(s) 116, 173, 501, 874, 949, 1091
AhdI GACNNNNNGTC 1 cut(s) 1045
AjnI CCWGG 2 cut(s) 579, 823
AluBI AGCT 5 cut(s) 22, 91, 160, 780, 1073
AluI AGCT 5 cut(s) 22, 91, 160, 780, 1073
Alw21I GWGCWC 1 cut(s) 925
Alw26I GTCTC 3 cut(s) 203, 532, 1183
AlwI GGATC 4 cut(s) 357, 370, 578, 591
AoxI GGCC 3 cut(s) 404, 821, 867
Asp700I GAANNNNTTC 1 cut(s) 1110
AspLEI GCGC 4 cut(s) 297, 613, 937, 1160
AspS9I GGNCC 2 cut(s) 867, 906
AsuC2I CCSGG 2 cut(s) 866, 1148
AsuHPI GGTGA 3 cut(s) 18, 319, 684
AvaII GGWCC 1 cut(s) 906
BamHI GGATCC 2 cut(s) 362, 583
BarI GAAGNNNNNNTAC 2 cut(s) 1098, 1130
BbrPI CACGTG 1 cut(s) 857
Bbv12I GWGCWC 1 cut(s) 925
BccI CCATC 1 cut(s) 354
BcgI CGANNNNNNTGC 2 cut(s) 1176, 1210
BciT130I CCWGG 2 cut(s) 581, 825
BcnI CCSGG 2 cut(s) 866, 1148
BcoDI GTCTC 3 cut(s) 203, 532, 1183
BfaI CTAG 3 cut(s) 72, 320, 374
BfmI CTRYAG 1 cut(s) 135
BglII AGATCT 1 cut(s) 312
BisI GCNGC 1 cut(s) 1185
BlsI GCNGC 1 cut(s) 1186
Bme1390I CCNGG 4 cut(s) 581, 825, 866, 1148
Bme18I GGWCC 1 cut(s) 906
BmeRI GACNNNNNGTC 1 cut(s) 1045
BmgT120I GGNCC 2 cut(s) 867, 906
BmiI GGNNCC 2 cut(s) 364, 585
BmrFI CCNGG 4 cut(s) 581, 825, 866, 1148
BmsI GCATC 3 cut(s) 315, 1169, 1196
BplI GAGNNNNNCTC 2 cut(s) 588, 620
BpmI CTGGAG 1 cut(s) 176
BpuEI CTTGAG 2 cut(s) 901, 1031
BpuMI CCSGG 2 cut(s) 866, 1148
BsaAI YACGTR 1 cut(s) 857
BsaHI GRCGYC 1 cut(s) 651
BsaI GGTCTC 2 cut(s) 532, 1183
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 159, 187
Bse3DI GCAATG 1 cut(s) 1050
BseBI CCWGG 2 cut(s) 581, 825
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMI GCAATG 1 cut(s) 1050
BseMII CTCAG 3 cut(s) 294, 795, 1194
BseNI ACTGG 2 cut(s) 159, 187
BseRI GAGGAG 1 cut(s) 32
BshFI GGCC 3 cut(s) 406, 823, 869
BsiHKAI GWGCWC 1 cut(s) 925
BsiSI CCGG 3 cut(s) 29, 865, 1147
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 3 cut(s) 203, 532, 1183
BsmI GAATGC 1 cut(s) 947
BsnI GGCC 3 cut(s) 406, 823, 869
Bso31I GGTCTC 2 cut(s) 532, 1183
Bsp1286I GDGCHC 1 cut(s) 925
Bsp143I GATC 5 cut(s) 312, 362, 583, 598, 896
BspACI CCGC 1 cut(s) 1185
BspANI GGCC 3 cut(s) 406, 823, 869
BspCNI CTCAG 3 cut(s) 295, 794, 1193
BspHI TCATGA 1 cut(s) 1098
BspLI GGNNCC 2 cut(s) 364, 585
BspMAI CTGCAG 1 cut(s) 139
BspPI GGATC 4 cut(s) 357, 370, 578, 591
BspQI GCTCTTC 1 cut(s) 502
BspTNI GGTCTC 2 cut(s) 532, 1183
BsrDI GCAATG 1 cut(s) 1050
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 2 cut(s) 159, 187
BssAI RCCGGY 1 cut(s) 28
BssMI GATC 5 cut(s) 312, 362, 583, 598, 896
BssNAI GTATAC 1 cut(s) 152
BssNI GRCGYC 1 cut(s) 651
Bst1107I GTATAC 1 cut(s) 152
Bst2UI CCWGG 2 cut(s) 581, 825
Bst4CI ACNGT 5 cut(s) 167, 276, 384, 927, 1060
Bst6I CTCTTC 1 cut(s) 502
BstACI GRCGYC 1 cut(s) 651
BstBAI YACGTR 1 cut(s) 857
BstC8I GCNNGC 6 cut(s) 408, 844, 848, 852, 1017, 1021
BstDEI CTNAG 4 cut(s) 303, 607, 781, 1180
BstHHI GCGC 4 cut(s) 297, 613, 937, 1160
BstKTI GATC 5 cut(s) 315, 365, 586, 601, 899
BstMAI GTCTC 3 cut(s) 203, 532, 1183
BstMBI GATC 5 cut(s) 312, 362, 583, 598, 896
BstMWI GCNNNNNNNGC 2 cut(s) 610, 866
BstNI CCWGG 2 cut(s) 581, 825
BstNSI RCATGY 1 cut(s) 1019
BstSCI CCNGG 4 cut(s) 579, 823, 864, 1146
BstSFI CTRYAG 1 cut(s) 135
BstX2I RGATCY 3 cut(s) 312, 362, 583
BstYI RGATCY 3 cut(s) 312, 362, 583
BstZ17I GTATAC 1 cut(s) 152
BsuRI GGCC 3 cut(s) 406, 823, 869
Cac8I GCNNGC 6 cut(s) 408, 844, 848, 852, 1017, 1021
CciI TCATGA 1 cut(s) 1098
CfoI GCGC 4 cut(s) 297, 613, 937, 1160
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 867, 906
CseI GACGC 1 cut(s) 640
Csp6I GTAC 1 cut(s) 545
CviAII CATG 4 cut(s) 395, 655, 1016, 1099
CviQI GTAC 1 cut(s) 545
DdeI CTNAG 4 cut(s) 303, 607, 781, 1180
DpnI GATC 5 cut(s) 314, 364, 585, 600, 898
DpnII GATC 5 cut(s) 312, 362, 583, 598, 896
DriI GACNNNNNGTC 1 cut(s) 1045
Eam1104I CTCTTC 1 cut(s) 502
Eam1105I GACNNNNNGTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 502
Eco147I AGGCCT 1 cut(s) 823
Eco31I GGTCTC 2 cut(s) 532, 1183
Eco47I GGWCC 1 cut(s) 906
Eco57I CTGAAG 3 cut(s) 32, 666, 744
Eco72I CACGTG 1 cut(s) 857
EcoRII CCWGG 2 cut(s) 579, 823
FaeI CATG 4 cut(s) 398, 658, 1019, 1102
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FatI CATG 4 cut(s) 394, 654, 1015, 1098
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 1185
Fsp4HI GCNGC 1 cut(s) 1185
FspBI CTAG 3 cut(s) 72, 320, 374
FspI TGCGCA 1 cut(s) 296
GlaI GCGC 4 cut(s) 296, 612, 936, 1159
GluI GCNGC 1 cut(s) 1185
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 3 cut(s) 406, 823, 869
HapII CCGG 3 cut(s) 29, 865, 1147
HgaI GACGC 1 cut(s) 640
HhaI GCGC 4 cut(s) 297, 613, 937, 1160
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 4 cut(s) 398, 658, 1019, 1102
Hin6I GCGC 4 cut(s) 295, 611, 935, 1158
HinP1I GCGC 4 cut(s) 295, 611, 935, 1158
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HindIII AAGCTT 1 cut(s) 1071
HinfI GANTC 6 cut(s) 7, 60, 211, 641, 689, 1040
HpaI GTTAAC 1 cut(s) 789
HpaII CCGG 3 cut(s) 29, 865, 1147
HphI GGTGA 3 cut(s) 18, 319, 684
Hpy166II GTNNAC 4 cut(s) 152, 427, 789, 1063
Hpy8I GTNNAC 4 cut(s) 152, 427, 789, 1063
HpyAV CCTTC 5 cut(s) 7, 641, 723, 758, 880
HpyCH4III ACNGT 5 cut(s) 167, 276, 384, 927, 1060
HpyCH4IV ACGT 3 cut(s) 148, 447, 856
HpyCH4V TGCA 5 cut(s) 137, 468, 842, 1023, 1055
HpyF10VI GCNNNNNNNGC 2 cut(s) 610, 866
HpyF3I CTNAG 4 cut(s) 303, 607, 781, 1180
HpySE526I ACGT 3 cut(s) 148, 447, 856
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 4 cut(s) 398, 658, 1019, 1102
HspAI GCGC 4 cut(s) 295, 611, 935, 1158
KspAI GTTAAC 1 cut(s) 789
Kzo9I GATC 5 cut(s) 312, 362, 583, 598, 896
LguI GCTCTTC 1 cut(s) 502
LmnI GCTCC 2 cut(s) 19, 157
LweI GCATC 3 cut(s) 315, 1169, 1196
MaeI CTAG 3 cut(s) 72, 320, 374
MaeII ACGT 3 cut(s) 148, 447, 856
MaeIII GTNAC 2 cut(s) 53, 223
MalI GATC 5 cut(s) 314, 364, 585, 600, 898
MboI GATC 5 cut(s) 312, 362, 583, 598, 896
MboII GAAGA 4 cut(s) 322, 489, 1071, 1210
MflI RGATCY 3 cut(s) 312, 362, 583
MhlI GDGCHC 1 cut(s) 925
MluCI AATT 1 cut(s) 988
MlyI GAGTC 2 cut(s) 205, 1034
MmeI TCCRAC 2 cut(s) 288, 1107
MnlI CCTC 6 cut(s) 10, 214, 298, 376, 407, 497
MroXI GAANNNNTTC 1 cut(s) 1110
MseI TTAA 4 cut(s) 39, 420, 788, 1032
MspI CCGG 3 cut(s) 29, 865, 1147
MspR9I CCNGG 4 cut(s) 581, 825, 866, 1148
Mva1269I GAATGC 1 cut(s) 947
MvaI CCWGG 2 cut(s) 581, 825
MwoI GCNNNNNNNGC 2 cut(s) 610, 866
NciI CCSGG 2 cut(s) 866, 1148
NdeII GATC 5 cut(s) 312, 362, 583, 598, 896
NlaIII CATG 4 cut(s) 398, 658, 1019, 1102
NlaIV GGNNCC 2 cut(s) 364, 585
NsbI TGCGCA 1 cut(s) 296
NspI RCATGY 1 cut(s) 1019
PaeI GCATGC 1 cut(s) 1019
PagI TCATGA 1 cut(s) 1098
PceI AGGCCT 1 cut(s) 823
PciSI GCTCTTC 1 cut(s) 502
PctI GAATGC 1 cut(s) 947
PdmI GAANNNNTTC 1 cut(s) 1110
PfeI GAWTC 4 cut(s) 7, 60, 641, 689
PkrI GCNGC 1 cut(s) 1186
PleI GAGTC 2 cut(s) 205, 1034
PmaCI CACGTG 1 cut(s) 857
PmlI CACGTG 1 cut(s) 857
PpsI GAGTC 2 cut(s) 205, 1034
Ppu21I YACGTR 1 cut(s) 857
Psp6I CCWGG 2 cut(s) 579, 823
PspCI CACGTG 1 cut(s) 857
PspGI CCWGG 2 cut(s) 579, 823
PspN4I GGNNCC 2 cut(s) 364, 585
PspPI GGNCC 2 cut(s) 867, 906
PstI CTGCAG 1 cut(s) 139
PsuI RGATCY 3 cut(s) 312, 362, 583
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
SapI GCTCTTC 1 cut(s) 502
SaqAI TTAA 4 cut(s) 39, 420, 788, 1032
SatI GCNGC 1 cut(s) 1185
Sau3AI GATC 5 cut(s) 312, 362, 583, 598, 896
Sau96I GGNCC 2 cut(s) 867, 906
SchI GAGTC 2 cut(s) 205, 1034
ScrFI CCNGG 4 cut(s) 581, 825, 866, 1148
SduI GDGCHC 1 cut(s) 925
SfaNI GCATC 3 cut(s) 315, 1169, 1196
SfcI CTRYAG 1 cut(s) 135
SinI GGWCC 1 cut(s) 906
SmlI CTYRAG 2 cut(s) 916, 1010
SmoI CTYRAG 2 cut(s) 916, 1010
SphI GCATGC 1 cut(s) 1019
Sse9I AATT 1 cut(s) 988
SseBI AGGCCT 1 cut(s) 823
SsiI CCGC 1 cut(s) 1185
SspI AATATT 1 cut(s) 176
SspMI CTAG 3 cut(s) 72, 320, 374
StuI AGGCCT 1 cut(s) 823
StyD4I CCNGG 4 cut(s) 579, 823, 864, 1146
TaaI ACNGT 5 cut(s) 167, 276, 384, 927, 1060
TaiI ACGT 3 cut(s) 151, 450, 859
TaqI TCGA 4 cut(s) 508, 595, 893, 1196
TasI AATT 1 cut(s) 988
TauI GCSGC 1 cut(s) 1187
TfiI GAWTC 4 cut(s) 7, 60, 641, 689
Tru1I TTAA 4 cut(s) 39, 420, 788, 1032
Tru9I TTAA 4 cut(s) 39, 420, 788, 1032
TspDTI ATGAA 4 cut(s) 368, 383, 789, 1087
TspGWI ACGGA 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 906
XbaI TCTAGA 1 cut(s) 71
XceI RCATGY 1 cut(s) 1019
XmiI GTMKAC 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 1110
XspI CTAG 3 cut(s) 72, 320, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.